scholarly journals mcr-1.2, a NewmcrVariant Carried on a Transferable Plasmid from a Colistin-Resistant KPC Carbapenemase-Producing Klebsiella pneumoniae Strain of Sequence Type 512

2016 ◽  
Vol 60 (9) ◽  
pp. 5612-5615 ◽  
Author(s):  
Vincenzo Di Pilato ◽  
Fabio Arena ◽  
Carlo Tascini ◽  
Antonio Cannatelli ◽  
Lucia Henrici De Angelis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Klebsiella Pneumoniae ◽  
Rectal Swab ◽  
Sequence Type ◽  
Content Type ◽  
Functional Variant ◽  
Leukemic Child

ABSTRACTA novelmcrvariant, namedmcr-1.2, encoding a Gln3-to-Leu functional variant of MCR-1, was detected in a KPC-3-producing ST512Klebsiella pneumoniaeisolate collected in Italy from a surveillance rectal swab from a leukemic child. Themcr-1.2gene was carried on a transferable IncX4 plasmid whose structure was very similar to that ofmcr-1-bearing plasmids previously found inEscherichia coliandK. pneumoniaestrains from geographically distant sites (Estonia, China, and South Africa).

scholarly journals Two Hypervirulent Klebsiella pneumoniae Isolates Producing a blaKPC-2 Carbapenemase from a Canadian Patient

2019 ◽  
Vol 63 (7) ◽  
Author(s):  
Laura F. Mataseje ◽  
David A. Boyd ◽  
Michael R. Mulvey ◽  
Yves Longtin
Keyword(s):  
Klebsiella Pneumoniae ◽  
Hospital Admission ◽  
Rectal Swab ◽  
Urine Culture ◽  
Sequence Type ◽  
Content Type ◽  
Canadian Patient

ABSTRACT This report describes two hypervirulent Klebsiella pneumoniae isolates that produced K. pneumoniae carbapenemase (KPC), which were identified from a rectal swab and a urine culture upon hospital admission. The patient had recently traveled to Greece, where he was hospitalized. The isolates were sequence type 86 and contained an IncHI1B IncFIBK hypervirulent plasmid and an IncFIIK plasmid harboring KPC.

scholarly journals Complete Sequence of a Conjugative IncN Plasmid HarboringblaKPC-2,blaSHV-12, andqnrS1from an Escherichia coli Sequence Type 648 Strain

2014 ◽  
Vol 58 (11) ◽  
pp. 6974-6977 ◽  
Author(s):  
Jun-Jie Li ◽  
Chang-Seop Lee ◽  
Ji-Fang Sheng ◽  
Yohei Doi
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Clinical History ◽  
Plasmid Profile ◽  
Complete Sequence ◽  
Sequence Type ◽  
Fluoroquinolone Resistance ◽  
Content Type

ABSTRACTWe sequenced a novel conjugativeblaKPC-2-harboring IncN plasmid, pYD626E, from anEscherichia colisequence type 648 strain previously identified in Pittsburgh, Pennsylvania. pYD626E was 72,800 bp long and carried four β-lactamase genes,blaKPC-2,blaSHV-12,blaLAP-1, andblaTEM-1. In addition, it harboredqnrS1(fluoroquinolone resistance) anddfrA14(trimethoprim resistance). The plasmid profile and clinical history supported thein vivotransfer of this plasmid betweenKlebsiella pneumoniaeandEscherichia coli.

scholarly journals Whole-Genome Sequence of a Novel Sequence Type 3136 Carbapenem-Resistant Klebsiella pneumoniae Strain Isolated from a Hospitalized Patient in Durban, South Africa

2018 ◽  
Vol 7 (23) ◽  
Author(s):  
Yogandree Ramsamy ◽  
Koleka P. Mlisana ◽  
Mushal Allam ◽  
Arshad Ismail ◽  
Ravesh Singh ◽  
...  
Keyword(s):  
South Africa ◽  
Klebsiella Pneumoniae ◽  
Virulence Factors ◽  
Genome Sequence ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Resistance Determinants ◽  
Carbapenem Resistant

Here, we describe the genome sequence of a novel sequence type 3136 (ST3136) Klebsiella pneumoniae strain isolated in South Africa. The 5,574,236-bp genome harbored 23 resistance determinants and 12 virulence factors that are of cardinal importance to infections.

scholarly journals Colistin Resistance Gene mcr-1 and Its Variant in Escherichia coli Isolates from Chickens in China

2017 ◽  
Vol 61 (5) ◽  
Author(s):  
Yong-Qiang Yang ◽  
Yun-Xia Li ◽  
Tao Song ◽  
Yan-Xian Yang ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Fold Increase ◽  
Sequence Type ◽  
Colistin Resistance ◽  
Content Type ◽  
Functional Variant

ABSTRACT The mcr-1 gene was detected in 5.11% (58/1136) of Escherichia coli isolates of chicken origin from 13 provinces in China. A novel mcr-1 variant, named mcr-1.3, encoding an Ile-to-Val functional variant of MCR-1 was identified in a sequence type 155 (ST155) strain. An mcr-1.3-containing IncI2 plasmid, pHeN867 (60,757 bp), was identified. The transfer of pHeN867 led to a 32-fold increase in the MIC of colistin in the recipient, exhibiting an effect on colistin resistance that was similar to that of mcr-1.

scholarly journals Various Sequence Types of Enterobacteriaceae Isolated from Commercial Chicken Farms in China and Carrying the blaNDM-5 Gene

2018 ◽  
Vol 62 (10) ◽  
Author(s):  
Rong Xiang ◽  
An-Yun Zhang ◽  
Xiao-Lan Ye ◽  
Zhuang-Zhuang Kang ◽  
Chang-Wei Lei ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Content Type ◽  
Commercial Chicken ◽  
Rectal Swabs ◽  
Sequence Types

ABSTRACT A total of 108 meropenem-resistant Enterobacteriaceae isolates were obtained from 1,658 rectal swabs collected from 15 unrelated commercial chicken farms in China between 2014 and 2016. These samples yielded 16 Escherichia coli and 2 Klebsiella pneumoniae isolates of diverse sequence types carrying a blaNDM-5-bearing IncX3 plasmid. K. pneumoniae strain sequence type 709 (ST709) has two blaNDM-5-carrying plasmids that were transferred together to E.coli.

scholarly journals blaNDM-5-Bearing IncFII-Type Plasmids of Klebsiella pneumoniae Sequence Type 147 Transmitted by Cross-Border Transfer of a Patient

2016 ◽  
Vol 60 (3) ◽  
pp. 1932-1934 ◽  
Author(s):  
Juyoun Shin ◽  
Jin Yang Baek ◽  
Sun Young Cho ◽  
Hee Jae Huh ◽  
Nam Yong Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
South Korea ◽  
United Arab Emirates ◽  
Sequence Type ◽  
Content Type ◽  
Korean Patient ◽  
Cross Border ◽  
Genetic Structures

The two plasmids extracted fromKlebsiella pneumoniaesequence type 147 (ST147) isolates were analyzed. The first isolate was obtained from a patient transferred from United Arab Emirates to South Korea. The second isolate was obtained from a Korean patient and was suspected to be transmitted from the first patient. Sequences of two plasmids were almost the same, and genetic structures, includingblaNDM-5, of these plasmids were similar to plasmids of NDM-1-producingEscherichia coliST131 isolates found in Europe.

scholarly journals A Plasmid Bearing theblaCTX-M-15Gene and Phage P1-Like Sequences from a Sequence Type 11 Klebsiella pneumoniae Isolate

2015 ◽  
Vol 59 (10) ◽  
pp. 6608-6610 ◽  
Author(s):  
Juyoun Shin ◽  
Kwan Soo Ko
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
South Korea ◽  
Resistance Genes ◽  
Sequence Type ◽  
Content Type ◽  
Plasmid Backbone

ABSTRACTPlasmid pKP12226 was extracted and analyzed from a CTX-M-15-producingKlebsiella pneumoniaesequence type 11 (ST11) isolate collected in South Korea. The plasmid represents chimeric characteristics consisting of a pIP1206-like backbone and lysogenized phage P1-like sequences. It bears a resistance region that includes resistance genes to several antibiotics and is different from previously characterized plasmids from South Korea bearingblaCTX-M-15. It may have resulted from recombination between anEscherichia coliplasmid backbone, ablaCTX-M-15-bearing resistance region, and lysogenized phage P1-like sequences.

scholarly journals Complete Sequences ofmcr-1-Harboring Plasmids from Extended-Spectrum-β-Lactamase- and Carbapenemase-Producing Enterobacteriaceae

2016 ◽  
Vol 60 (7) ◽  
pp. 4351-4354 ◽  
Author(s):  
Aiqing Li ◽  
Yong Yang ◽  
Minhui Miao ◽  
Kalyan D. Chavda ◽  
José R. Mediavilla ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Clinical Isolates ◽  
Incompatibility Group ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Complete Sequences ◽  
Group A

ABSTRACTHere we completely sequenced fourmcr-1-haboring plasmids, isolated from two extended-spectrum-β-lactamase (ESBL)-producingEscherichia coliand two carbapenemase-producingKlebsiella pneumoniaeclinical isolates. Themcr-1-harboring plasmids from anE. colisequence type 2448 (ST2448) isolate and twoK. pneumoniaeST25 isolates were identical (all pMCR1-IncX4), belonging to the IncX4 incompatibility group, while the plasmid from anE. coliST2085 isolate (pMCR1-IncI2) belongs to the IncI2 group. A nearly identical 2.6-kbmcr-1-pap2element was found to be shared by allmcr-1-carrying plasmids.

A multi-institutional outbreak of New Delhi metallo-β-lactamase–producing Escherichia coli with subsequent acquisition of the Klebsiella pneumoniae carbapenemase gene

2020 ◽  
pp. 1-4
Author(s):  
Ester Solter ◽  
Jason C. Kwong ◽  
Aaron Walton ◽  
Norelle Sherry ◽  
Benjamin P. Howden ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Common Ancestor ◽  
Sequence Type ◽  
Second Phase ◽  
New Delhi ◽  
Genetic Sequencing ◽  
Klebsiella Pneumoniae Carbapenemase ◽  
Carbapenemase Gene

Abstract We characterized 57 isolates from a 2-phase clonal outbreak of New Delhi metallo-β-lactamase–producing Eschericha coli, involving 9 Israeli hospitals; all but 1 isolate belonged to sequence-type (ST) 410. Most isolates in the second phase harbored blaKPC-2 in addition to blaNDM-5. Genetic sequencing revealed most dual-carbapenemase–producing isolates to be monophyletically derived from a common ancestor.

scholarly journals Colistin Resistance in Carbapenem-Resistant Klebsiella pneumoniae Mediated by Chromosomal Integration of Plasmid DNA

2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Astrid V. Cienfuegos-Gallet ◽  
Liang Chen ◽  
Barry N. Kreiswirth ◽  
J. Natalia Jiménez
Keyword(s):  
Klebsiella Pneumoniae ◽  
Plasmid Dna ◽  
Clonal Expansion ◽  
Genetic Alterations ◽  
Sequence Type ◽  
Chromosomal Integration ◽  
Colistin Resistance ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Single Locus Variant

ABSTRACT Here we describe the spread of colistin resistance in clinical isolates of carbapenem-resistant Klebsiella pneumoniae in Medellín, Colombia. Among 32 isolates collected between 2012 and 2014, 24 showed genetic alterations in mgrB. Nineteen isolates belonged to sequence type 512 (ST512) (or its single locus variant [SLV]) and harbored an 8.1-kb hsdMSR insertion corresponding to ISKpn25, indicating a clonal expansion of the resistant strain. The insertion region showed 100% identity to several plasmids, suggesting that the colistin resistance is mediated by chromosomal integration of plasmid DNA.

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