scholarly journals The Complete Nucleotide Sequence of the Carbapenem Resistance-Conferring Conjugative Plasmid pLD209 from a Pseudomonas putida Clinical Strain Reveals a Chimeric Design Formed by Modules Derived from Both Environmental and Clinical Bacteria

2014 ◽  
Vol 58 (3) ◽  
pp. 1816-1821 ◽  
Author(s):  
Patricia M. Marchiaro ◽  
Luciano Brambilla ◽  
Jorgelina Morán-Barrio ◽  
Santiago Revale ◽  
Fernando Pasteran ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Carbapenem Resistance ◽  
Complete Sequence ◽  
Conjugative Plasmid ◽  
Clinical Strain ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
Environmental Bacteria ◽  
Replication Module

ABSTRACTThe complete sequence of the carbapenem-resistance-conferring conjugative plasmid pLD209 from aPseudomonas putidaclinical strain is presented. pLD209 is formed by 3 well-defined regions: an adaptability module encompassing a Tn402-like class 1 integron of clinical origin containingblaVIM-2andaacA4gene cassettes, partitioning and transfer modules, and a replication module derived from plasmids of environmental bacteria. pLD209 is thus a mosaic of modules originating in both the clinical and environmental (nonclinical) microbiota.

scholarly journals Complete Genome Sequence of Pseudomonas aeruginosa CMC-097, Isolated from a Ventilator-Associated Pneumonia Patient, Containing a Novel Carbapenem Resistance Class 1 Integron

2021 ◽  
Vol 10 (35) ◽  
Author(s):  
Jayasimha Rao ◽  
Adenike Adenikinju ◽  
Thomas M. Kerkering ◽  
Dorothy C. Garner ◽  
Roderick V. Jensen
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Complete Genome ◽  
Carbapenem Resistance ◽  
Clinical Strain ◽  
Ventilator Associated Pneumonia ◽  
Class 1 Integron ◽  
Circular Chromosome ◽  
Content Type ◽  
Pneumonia Patient ◽  
Class 1

We report the complete genome of a clinical strain of Pseudomonas aeruginosa , CMC-097, which was isolated from a ventilator-associated pneumonia patient with a chronic infection. Illumina sequence reads were assembled using Geneious to yield a 7,044,064-bp circular chromosome containing a carbapenem resistance integron, In 2020 .

scholarly journals Complete Sequence of pOZ176, a 500-Kilobase IncP-2 Plasmid Encoding IMP-9-Mediated Carbapenem Resistance, from Outbreak Isolate Pseudomonas aeruginosa 96

2013 ◽  
Vol 57 (8) ◽  
pp. 3775-3782 ◽  
Author(s):  
Jianhui Xiong ◽  
David C. Alexander ◽  
Jennifer H. Ma ◽  
Maxime Déraspe ◽  
Donald E. Low ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbapenem Resistance ◽  
Complete Sequence ◽  
Open Reading Frames ◽  
Content Type ◽  
Conserved Sequence ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Usage Analysis ◽  
Type 3

ABSTRACTPseudomonas aeruginosa96 (PA96) was isolated during a multicenter surveillance study in Guangzhou, China, in 2000. Whole-genome sequencing of this outbreak strain facilitated analysis of its IncP-2 carbapenem-resistant plasmid, pOZ176. The plasmid had a length of 500,839 bp and an average percent G+C content of 57%. Of the 618 predicted open reading frames, 65% encode hypothetical proteins. The pOZ176 backbone is not closely related to any plasmids thus far sequenced, but some similarity to pQBR103 ofPseudomonas fluorescensSBW25 was observed. Two multiresistant class 1 integrons and several insertion sequences were identified. TheblaIMP-9-carrying integron containedaacA4→blaIMP-9→aacA4, flanked upstream by Tn21 tnpMRAand downstream by a completetnioperon of Tn402and amermodule, named Tn6016. The second integron carriedaacA4→catB8a→blaOXA-10and was flanked by Tn1403-liketnpRAand asul1-type 3′ conserved sequence (3′-CS), named Tn6217. Other features include three resistance genes similar to those of Tn5, a tellurite resistance operon, and twopiloperons. The replication and maintenance systems exhibit similarity to a genomic island ofRalstonia solanacearumGM1000. Codon usage analysis suggests the recent acquisition ofblaIMP-9. The origins of the integrons on pOZ176 indicated separate horizontal gene transfer events driven by antibiotic selection. The novel mosaic structure of pOZ176 suggests that it is derived from environmental bacteria.

scholarly journals First Description of KPC-2-Producing Pseudomonas putida in Brazil

2012 ◽  
Vol 56 (4) ◽  
pp. 2205-2206 ◽  
Author(s):  
Anna C. S. Almeida ◽  
Marinalda A. Vilela ◽  
Felipe L. S. Cavalcanti ◽  
Willames M. B. S. Martins ◽  
Marcos A. Morais ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Pseudomonas Putida ◽  
Polymyxin B ◽  
Class 1 Integron ◽  
Content Type ◽  
First Case ◽  
Class 1

ABSTRACTThis work reports the identification of the first case of a KΡC-2-producingPseudomonas putidaisolate (PP36) in Brazil. The PP36 isolate was resistant to all the antimicrobials tested except polymyxin B. In addition to the discoveredblaKPC-2gene, genetic analysis showed the presence of a class 1 integron containing thedhfrXVb gene and the new allelearr-6, which codes for resistance to rifampin. These elements were found in an IncFI 65-kb plasmid.

scholarly journals First Report ofblaIMP-14on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (8) ◽  
pp. 5068-5071 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert P. Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTheblaIMP-14carbapenem resistance gene has largely previously been observed inPseudomonas aeruginosaandAcinetobacterspp. As part of global surveillance and sequencing of carbapenem-resistantEscherichia coli, we identified a sequence type 131 strain harboringblaIMP-14within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2plasmid. The emergence ofblaIMP-14in this context in the ST131 lineage is of potential clinical concern.

scholarly journals Molecular and Biochemical Characterization of CTX-M-131, a Natural Asp240Gly Variant Derived from CTX-M-2, Produced by a Providencia rettgeri Clinical Strain in São Paulo, Brazil

2015 ◽  
Vol 59 (3) ◽  
pp. 1815-1817 ◽  
Author(s):  
Milena Dropa ◽  
Barbara Ghiglione ◽  
Maria Helena Matté ◽  
Livia Carminato Balsalobre ◽  
Nilton Lincopan ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Clinical Strain ◽  
Class 1 Integron ◽  
Providencia Rettgeri ◽  
Content Type ◽  
Steady State Kinetic ◽  
Class 1 ◽  
Encoding Genes ◽  
State Kinetic

ABSTRACTCTX-M-131 is a natural Asp240Gly variant from the CTX-M-2 group detected in aProvidencia rettgericlinical strain from Brazil. Molecular analysis showed thatblaCTX-M-131was inserted in a complex class 1 integron harbored by a 112-kb plasmid, which has not been previously described as a platform for CTX-M-encoding genes with the Asp240Gly mutation. Steady-state kinetic parameters showed that the enzyme has a typical cefotaximase catalytic profile and an enhanced activity against ceftazidime.

scholarly journals OXA-198, an Acquired Carbapenem-Hydrolyzing Class D β-Lactamase from Pseudomonas aeruginosa

2011 ◽  
Vol 55 (10) ◽  
pp. 4828-4833 ◽  
Author(s):  
Farid El Garch ◽  
Pierre Bogaerts ◽  
Carine Bebrone ◽  
Moreno Galleni ◽  
Youri Glupczynski
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbapenem Resistance ◽  
Amino Acid Identity ◽  
Ventilator Associated Pneumonia ◽  
Class 1 Integron ◽  
Content Type ◽  
New Class ◽  
Carbapenem Resistant ◽  
Class D ◽  
Class 1

ABSTRACTA carbapenem-resistantPseudomonas aeruginosastrain (PA41437) susceptible to expanded-spectrum cephalosporins was recovered from several consecutive lower-respiratory-tract specimens of a patient who developed a ventilator-associated pneumonia while hospitalized in an intensive care unit. Cloning experiments identified OXA-198, a new class D β-lactamase which was weakly related (less than 45% amino acid identity) to other class D β-lactamases. Expression inEscherichia coliTOP10 and inP. aeruginosaPAO1 led to transformants that were resistant to ticarcillin and showed reduced susceptibility to carbapenems and cefepime. TheblaOXA-198gene was harbored by a class 1 integron carried by a ca. 46-kb nontypeable plasmid. This study describes a novel class D β-lactamase involved in carbapenem resistance inP. aeruginosa.

scholarly journals Environmental Microbiota Represents a Natural Reservoir for Dissemination of Clinically Relevant Metallo-β-Lactamases

2011 ◽  
Vol 55 (11) ◽  
pp. 5376-5379 ◽  
Author(s):  
Claudia Scotta ◽  
Carlos Juan ◽  
Gabriel Cabot ◽  
Antonio Oliver ◽  
Jorge Lalucat ◽  
...  
Keyword(s):  
Sewage Water ◽  
Ochrobactrum Anthropi ◽  
Class 1 Integron ◽  
Content Type ◽  
Natural Reservoir ◽  
Brevundimonas Diminuta ◽  
Pseudomonas Monteilii ◽  
Class 1 ◽  
Enterobacter Ludwigii ◽  
Environmental Bacteria

ABSTRACTA total of 10 metallo-β-lactamase-producing isolates of six different species, includingBrevundimonas diminuta(n= 3),Rhizobium radiobacter(n= 2),Pseudomonas monteilii(n= 1),Pseudomonas aeruginosa(n= 2),Ochrobactrum anthropi(n= 1), andEnterobacter ludwigii(n= 1), were detected in the sewage water of a hospital. The presence ofblaVIM-13associated with a Tn1721-class 1 integron structure was detected in all but one of the isolates (E. ludwigii, which produced VIM-2), and in two of them (R. radiobacter), this structure was located on a plasmid, suggesting that environmental bacteria represent a reservoir for the dissemination of clinically relevant metallo-β-lactamase genes.

scholarly journals Complete Genome Sequencing of Acinetobacter baumannii Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Daniel Wibberg ◽  
Ileana P. Salto ◽  
Felix G. Eikmeyer ◽  
Irena Maus ◽  
Anika Winkler ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Genome Sequencing ◽  
Antibiotic Resistance Gene ◽  
Insertion Site ◽  
Multidrug Resistant ◽  
Conjugative Plasmid ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenemase Gene ◽  
Class 1

ABSTRACT Multidrug-resistant (MDR) Acinetobacter baumannii strains appeared as serious emerging nosocomial pathogens in clinical environments and especially in intensive care units (ICUs). A. baumannii strain K50, recovered from a hospitalized patient in Kuwait, exhibited resistance to carbapenems and additionally to ciprofloxacin, chloramphenicol, sulfonamides, amikacin, and gentamicin. Genome sequencing revealed that the strain possesses two plasmids, pK50a (79.6 kb) and pK50b (9.5 kb), and a 3.75-Mb chromosome. A. baumannii K50 exhibits an average nucleotide identity (ANI) of 99.98% to the previously reported Iraqi clinical isolate AA-014, even though the latter strain lacked plasmid pK50a. Strain K50 belongs to sequence type 158 (ST158) (Pasteur scheme) and ST499 (Oxford scheme). Plasmid pK50a is a member of the Aci6 (replication group 6 [RG6]) group of Acinetobacter plasmids and carries a conjugative transfer module and two antibiotic resistance gene regions. The transposon Tn 2008 carries the carbapenemase gene bla OXA-23 , whereas a class 1 integron harbors the resistance genes bla GES-11 , aacA4 , dfrA7 , qacE Δ 1 , and sul1 , conferring resistance to all β-lactams and reduced susceptibility to carbapenems and resistance to aminoglycosides, trimethoprim, quaternary ammonium compounds, and sulfamethoxazole, respectively. The class 1 integron is flanked by MITEs (miniature inverted-repeat transposable elements) delimiting the element at its insertion site.

scholarly journals IMP-29, a Novel IMP-Type Metallo-β-Lactamase in Pseudomonas aeruginosa

2012 ◽  
Vol 56 (4) ◽  
pp. 2187-2190 ◽  
Author(s):  
Katy Jeannot ◽  
Laurent Poirel ◽  
Marjorie Robert-Nicoud ◽  
Pascal Cholley ◽  
Patrice Nordmann ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biochemical Analysis ◽  
Gene Cassette ◽  
Amino Acid Identity ◽  
The Other ◽  
Conjugative Plasmid ◽  
Class 1 Integron ◽  
Content Type ◽  
Acid Identity ◽  
Class 1

ABSTRACTAnalysis of two clonally related multiresistantPseudomonas aeruginosaisolates led to the identification of a novel IMP-type metallo-β-lactamase. IMP-29 was significantly different from the other IMP variants (the closest variant being IMP-5 with 93% amino acid identity). TheblaIMP-29gene cassette was carried by a class 1 integron in strain 10.298, while in strain 10.266 it was located in a rearranged DNA region on a 30-kb conjugative plasmid. Biochemical analysis confirmed that IMP-29 efficiently hydrolyzed carbapenems.

scholarly journals Dissemination of Multidrug-Resistant Proteus mirabilis Clones Carrying a Novel Integron-Borne blaIMP-1 in a Tertiary Hospital

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
Ana Carolina Ramos ◽  
Rodrigo Cayô ◽  
Cecilia Godoy Carvalhaes ◽  
Thomas Jové ◽  
Greice Pereira da Silva ◽  
...  
Keyword(s):  
Infection Control ◽  
Proteus Mirabilis ◽  
Tertiary Hospital ◽  
Multidrug Resistant ◽  
Conjugative Plasmid ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACT This study aimed to characterize multidrug-resistant Proteus mirabilis clones carrying a novel class 1 integron-borne bla IMP-1. In1359 was inserted into a large conjugative plasmid that also carried bla CTX-M-2. The production of carbapenemases in Enterobacteriaceae that are intrinsically resistant to polymyxins and tigecycline is very worrisome, representing a serious challenge to clinicians and infection control teams.

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