scholarly journals Dissemination of Multidrug-Resistant Proteus mirabilis Clones Carrying a Novel Integron-Borne blaIMP-1 in a Tertiary Hospital

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
Ana Carolina Ramos ◽  
Rodrigo Cayô ◽  
Cecilia Godoy Carvalhaes ◽  
Thomas Jové ◽  
Greice Pereira da Silva ◽  
...  
Keyword(s):  
Infection Control ◽  
Proteus Mirabilis ◽  
Tertiary Hospital ◽  
Multidrug Resistant ◽  
Conjugative Plasmid ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACT This study aimed to characterize multidrug-resistant Proteus mirabilis clones carrying a novel class 1 integron-borne bla IMP-1. In1359 was inserted into a large conjugative plasmid that also carried bla CTX-M-2. The production of carbapenemases in Enterobacteriaceae that are intrinsically resistant to polymyxins and tigecycline is very worrisome, representing a serious challenge to clinicians and infection control teams.

scholarly journals Complete Genome Sequencing of Acinetobacter baumannii Strain K50 Discloses the Large Conjugative Plasmid pK50a Encoding Carbapenemase OXA-23 and Extended-Spectrum β-Lactamase GES-11

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Daniel Wibberg ◽  
Ileana P. Salto ◽  
Felix G. Eikmeyer ◽  
Irena Maus ◽  
Anika Winkler ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Genome Sequencing ◽  
Antibiotic Resistance Gene ◽  
Insertion Site ◽  
Multidrug Resistant ◽  
Conjugative Plasmid ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenemase Gene ◽  
Class 1

ABSTRACT Multidrug-resistant (MDR) Acinetobacter baumannii strains appeared as serious emerging nosocomial pathogens in clinical environments and especially in intensive care units (ICUs). A. baumannii strain K50, recovered from a hospitalized patient in Kuwait, exhibited resistance to carbapenems and additionally to ciprofloxacin, chloramphenicol, sulfonamides, amikacin, and gentamicin. Genome sequencing revealed that the strain possesses two plasmids, pK50a (79.6 kb) and pK50b (9.5 kb), and a 3.75-Mb chromosome. A. baumannii K50 exhibits an average nucleotide identity (ANI) of 99.98% to the previously reported Iraqi clinical isolate AA-014, even though the latter strain lacked plasmid pK50a. Strain K50 belongs to sequence type 158 (ST158) (Pasteur scheme) and ST499 (Oxford scheme). Plasmid pK50a is a member of the Aci6 (replication group 6 [RG6]) group of Acinetobacter plasmids and carries a conjugative transfer module and two antibiotic resistance gene regions. The transposon Tn 2008 carries the carbapenemase gene bla OXA-23 , whereas a class 1 integron harbors the resistance genes bla GES-11 , aacA4 , dfrA7 , qacE Δ 1 , and sul1 , conferring resistance to all β-lactams and reduced susceptibility to carbapenems and resistance to aminoglycosides, trimethoprim, quaternary ammonium compounds, and sulfamethoxazole, respectively. The class 1 integron is flanked by MITEs (miniature inverted-repeat transposable elements) delimiting the element at its insertion site.

scholarly journals PGI2 Is a Novel SGI1-Relative Multidrug-Resistant Genomic Island Characterized in Proteus mirabilis

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Chang-Wei Lei ◽  
Yan-Peng Chen ◽  
Ling-Han Kong ◽  
Jin-Xin Zeng ◽  
Yong-Xiang Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Proteus Mirabilis ◽  
Genomic Island ◽  
Multidrug Resistant ◽  
Complex Class ◽  
Helper Plasmid ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACT A novel 61,578-bp genomic island named Proteus genomic island 2 (PGI2) was characterized in Proteus mirabilis of swine origin in China. The 23.85-kb backbone of PGI2 is related to those of Salmonella genomic island 1 and Acinetobacter genomic island 1. The multidrug resistance (MDR) region of PGI2 is a complex class 1 integron containing 14 different resistance genes. PGI2 was conjugally mobilized in trans to Escherichia coli in the presence of a conjugative IncC helper plasmid.

scholarly journals Isolation of VIM-2-Producing Pseudomonas monteilii Clinical Strains Disseminated in a Tertiary Hospital in Northern Spain

2014 ◽  
Vol 59 (2) ◽  
pp. 1334-1336 ◽  
Author(s):  
Alain A. Ocampo-Sosa ◽  
Laura P. Guzmán-Gómez ◽  
Marta Fernández-Martínez ◽  
Elena Román ◽  
Cristina Rodríguez ◽  
...  
Keyword(s):  
Tertiary Hospital ◽  
Multidrug Resistant ◽  
University Hospital ◽  
Clinical Samples ◽  
Northern Spain ◽  
Class 1 Integron ◽  
Content Type ◽  
Resistant Species ◽  
Pseudomonas Monteilii ◽  
Class 1

ABSTRACTWe describe here the occurrence ofblaVIM-2in 10 carbapenem-resistantPseudomonas monteiliistrains isolated from different clinical samples from patients at the University Hospital Marqués de Valdecilla in northern Spain. All theblaVIM-2-harboringP. monteiliiisolates possessed a class 1 integron, with the cassette array [intI1_blaVIM-2_aac(6′)-Ib_qacEΔ1_sul1]. Our results show the emergence of VIM-2-producing multidrug-resistant species other thanPseudomonas aeruginosaorPseudomonas putidain a Spanish hospital.P. monteilii, although sporadically isolated, should also be considered an important metallo-β-lactamase (MBL) reservoir.

scholarly journals Salmonella enterica Serovar Typhi Strains Isolated in Korea Containing a Multidrug Resistance Class 1 Integron

2003 ◽  
Vol 47 (6) ◽  
pp. 2006-2008 ◽  
Author(s):  
Hyunjoo Pai ◽  
Jeong-hum Byeon ◽  
Sunmi Yu ◽  
Bok Kwon Lee ◽  
Shukho Kim
Keyword(s):  
Multidrug Resistance ◽  
Resistance Genes ◽  
Salmonella Enterica ◽  
Multidrug Resistant ◽  
Conjugative Plasmid ◽  
The Novel ◽  
Class 1 Integron ◽  
Salmonella Enterica Serovar Typhi ◽  
Class 1 ◽  
Clonal Spread

ABSTRACT Six strains of Salmonella enterica serovar Typhi which were resistant to ampicillin, chloramphenicol, trimethoprim-sulfamethoxazole, streptomycin, tetracycline, and gentamicin were isolated in Korea. This multidrug resistance was transferred by a conjugative plasmid of about 50 kb. The plasmid harbored a class 1 integron, which included six resistance genes, aacA4b, catB8, aadA1, dfrA1, aac(6′)-IIa, and the novel blaP2, in that order. All of the isolates showed the same-size plasmids and the same ribotyping patterns, which suggests a clonal spread of these multidrug-resistant isolates.

scholarly journals Diversity and Evolution of AbaR Genomic Resistance Islands in Acinetobacter baumannii Strains of European Clone I

2011 ◽  
Vol 55 (7) ◽  
pp. 3201-3206 ◽  
Author(s):  
Lenka Krizova ◽  
Lenie Dijkshoorn ◽  
Alexandr Nemec
Keyword(s):  
Acinetobacter Baumannii ◽  
Selective Advantage ◽  
Multidrug Resistant ◽  
Original Form ◽  
Class 1 Integron ◽  
Content Type ◽  
Atpase Gene ◽  
Class 1 ◽  
Major Clone ◽  
Derivatives Of

ABSTRACTTo assess the diversity of AbaR genomic resistance islands inAcinetobacter baumanniiEuropean clone I (MLST clonal complex 1), we investigated 26 multidrug-resistant strains of this major clone isolated from hospitals in 21 cities of 10 European countries between 1984 and 2005. Each strain harbored an AbaR structure integrated at the same position in the chromosomal ATPase gene. AbaR3, including four subtypes based on variations in class 1 integron cassettes, and AbaR10 were found in 15 and 2 strains, respectively, whereas a new, unique AbaR variant was discovered in each of the other 9 strains. These new variants, designated AbaR11 to AbaR19 (19.8 kb to 57.5 kb), seem to be truncated derivatives of AbaR3, likely resulting from the deletions of its internal parts mediated by either IS26elements (AbaR12 to AbaR19) or homologous recombination (AbaR11). AbaR3 was detected in all 10 strains isolated in 1984 to 1991, while AbaR11 to AbaR19 were carried only by strains isolated since 1997. Our results and those from previous publications suggest that AbaR3 is the original form of AbaR in European clone I, which may have provided strains of the lineage with a selective advantage facilitating their spread in European hospitals in the 1980s or before.

Antibiotic Resistance Conferred by a Conjugative Plasmid and a Class I Integron in Vibrio cholerae O1 El Tor Strains Isolated in Albania and Italy

1999 ◽  
Vol 43 (3) ◽  
pp. 693-696 ◽  
Author(s):  
Vincenzo Falbo ◽  
Alessandra Carattoli ◽  
Fabio Tosini ◽  
Cristina Pezzella ◽  
Anna Maria Dionisi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Cholerae ◽  
Molecular Basis ◽  
Gene Cassette ◽  
Multidrug Resistant ◽  
Conjugative Plasmid ◽  
Class 1 Integron ◽  
Class 1 ◽  
Vibrio Cholerae O1 ◽  
El Tor

ABSTRACT Multidrug-resistant Vibrio cholerae O1 El Tor strains isolated during the 1994 outbreak of cholera in Albania and Italy were characterized for the molecular basis of antibiotic resistance. All strains were found to be resistant to tetracycline, streptomycin, spectinomycin, trimethoprim, sulfathiazole, and the vibriostatic compound O/129 (2,4-diamino-6,7-diisopropylteridine). Resistance genes were self-transferable by a conjugative plasmid of about 60 MDa, with the exception of spectinomycin resistance, which was conferred by theaadA1 gene cassette located in the bacterial chromosome within a class 1 integron. The resistance to trimethoprim and O/129 was conferred by the dfrA1 gene, which was present on the plasmid. Although the dfrA1 gene is known to be borne on an integron cassette, class 1, 2, or 3 intI genes were not detected as part of the plasmid DNA from the strains studied.

scholarly journals First Occurrence of the OXA-198 Carbapenemase in Enterobacterales

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Rémy A. Bonnin ◽  
Agnès B. Jousset ◽  
Lauraine Gauthier ◽  
Cécile Emeraud ◽  
Delphine Girlich ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Routine Screening ◽  
Conjugative Plasmid ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Class 1 Integron ◽  
Carbapenem Resistant ◽  
Carbapenemase Gene ◽  
Class 1 ◽  
First Occurrence

ABSTRACT A carbapenem-resistant Citrobacter sp. was recovered from routine screening of multidrug-resistant bacteria. This isolate coproduced OXA-48 and OXA-198. OXA-48 was carried by the prototypical IncL plasmid, whereas OXA-198 was carried by a peculiar IncHI-type plasmid. This carbapenemase gene was inserted within a class 1 integron located on a conjugative plasmid. This report describes the first occurrence of OXA-198 in Enterobacterales.

scholarly journals Two New SGI1-LK Variants Found in Proteus mirabilis and Evolution of the SGI1-HKL Group of Salmonella Genomic Islands

mSphere ◽  
2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Claire de Curraize ◽  
Eliane Siebor ◽  
Véronique Varin ◽  
Catherine Neuwirth ◽  
Ruth M. Hall
Keyword(s):  
Homologous Recombination ◽  
Proteus Mirabilis ◽  
Salmonella Enterica ◽  
Genomic Islands ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Content Type ◽  
Novel Variants ◽  
Class 1 ◽  
Complex Forms

ABSTRACT Integrative mobilizable elements belonging to the SGI1-H, -K, and -L Salmonella genomic island 1 (SGI1) variant groups are distinguished by the presence of an alteration in the backbone (IS1359 replaces 2.8 kb of the backbone extending from within traN [S005] to within S009). Members of this SGI1-HKL group have been found in Salmonella enterica serovars and in Proteus mirabilis. Two novel variants from this group, designated SGI1-LK1 and SGI1-LK2, were found in the draft genomes of antibiotic-resistant P. mirabilis isolates from two French hospitals. Both variants can be derived from SGI1-PmGUE, a configuration found previously in another P. mirabilis isolate from France. SGI1-LK1 could arise via an IS26-mediated inversion in the complex class 1 integron that duplicated the IS26 element and the target site in IS6100. SGI1-LK1 also has a larger 8.59-kb backbone deletion extending from traN to within S013 and removing traG and traH. However, SGI1-LK1 was mobilized by an IncC plasmid. SGI1-LK2 can be derived from a hypothetical progenitor, SGI1-LK0, that is related to SGI1-PmGUE but lacks the aphA1 gene and one copy of IS26. The integron of SGI1-LK2 could arise via deletion of DNA adjacent to an IS26 and a deletion occurring via homologous recombination between duplicated copies of part of the integron 3′-conserved segment. SGI1-K can also be derived from SGI1-LK0. This would involve an IS26-mediated deletion and an inversion via homologous recombination of a segment between inversely oriented IS26s. Similar events can explain the configuration of the integrons in other SGI1-LK variants. IMPORTANCE Members of the SGI1-HKL subgroup of SGI1-type integrative mobilizable elements have a characteristic alteration in their backbone. They are widely distributed among multiply antibiotic-resistant Salmonella enterica serovars and Proteus mirabilis isolates. The SGI1-K type, found in the globally disseminated multiply antibiotic-resistant Salmonella enterica serovar Kentucky clone ST198 (sequence type 198), and various configurations in the original SGI1-LK group, found in other multiresistant S. enterica serovars and Proteus mirabilis isolates, have complex and highly plastic resistance regions due to the presence of IS26. However, how these complex forms arose and the relationships between them had not been analyzed. Here, a hypothetical progenitor, SGI1-LK0, that can be formed from the simpler SGI1-H is proposed, and the pathways to the formation of new variants, SGI1-LK1 and SGI1-LK2, found in P. mirabilis and other reported configurations via homologous recombination and IS26-mediated events are proposed. This led to a better understanding of the evolution of the SGI1-HKL group.

scholarly journals Molecular Epidemiology and Genetic Characteristics of VariousblaPERGenes in Shanghai, China

2016 ◽  
Vol 60 (6) ◽  
pp. 3849-3853 ◽  
Author(s):  
Lianyan Xie ◽  
Jun Wu ◽  
Fangfang Zhang ◽  
Lizhong Han ◽  
Xiaokui Guo ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Inverted Repeat ◽  
Transmission Mechanism ◽  
The Other ◽  
Complex Class ◽  
Class 1 Integron ◽  
Genetic Characteristics ◽  
Gram Negative ◽  
Content Type ◽  
Class 1

We describe the genetic characteristics and possible transmission mechanism ofblaPERin 25 clinical Gram-negative bacilli in Shanghai.blaPER, includingblaPER-1,blaPER-3, andblaPER-4, was located chromosomally or in different plasmids. Tn1213harboringblaPER-1was first identified in twoProteus mirabilisisolates in China. The otherblaPERvariants were preceded by an ISCR1element inside the complex class 1 integron associated with IS26, Tn21, Tn1696, and a miniature inverted-repeat transposable element.

scholarly journals The Complete Nucleotide Sequence of the Carbapenem Resistance-Conferring Conjugative Plasmid pLD209 from a Pseudomonas putida Clinical Strain Reveals a Chimeric Design Formed by Modules Derived from Both Environmental and Clinical Bacteria

2014 ◽  
Vol 58 (3) ◽  
pp. 1816-1821 ◽  
Author(s):  
Patricia M. Marchiaro ◽  
Luciano Brambilla ◽  
Jorgelina Morán-Barrio ◽  
Santiago Revale ◽  
Fernando Pasteran ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Carbapenem Resistance ◽  
Complete Sequence ◽  
Conjugative Plasmid ◽  
Clinical Strain ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
Environmental Bacteria ◽  
Replication Module

ABSTRACTThe complete sequence of the carbapenem-resistance-conferring conjugative plasmid pLD209 from aPseudomonas putidaclinical strain is presented. pLD209 is formed by 3 well-defined regions: an adaptability module encompassing a Tn402-like class 1 integron of clinical origin containingblaVIM-2andaacA4gene cassettes, partitioning and transfer modules, and a replication module derived from plasmids of environmental bacteria. pLD209 is thus a mosaic of modules originating in both the clinical and environmental (nonclinical) microbiota.

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