scholarly journals Comparison of Simultaneous Splenic Sample PCR with Blood Sample PCR for Diagnosis and Treatment of Experimental Ehrlichia canis Infection

2004 ◽  
Vol 48 (11) ◽  
pp. 4488-4490 ◽  
Author(s):  
Shimon Harrus ◽  
Martin Kenny ◽  
Limor Miara ◽  
Itzhak Aizenberg ◽  
Trevor Waner ◽  
...  
Keyword(s):  
Body Weight ◽  
Early Diagnosis ◽  
Blood Sample ◽  
Ehrlichia Canis ◽  
Diagnosis And Treatment ◽  
Doxycycline Treatment ◽  
Canine Monocytic Ehrlichiosis

ABSTRACT This report presents evidence that dogs recover from acute canine monocytic ehrlichiosis (CME) after 16 days of doxycycline treatment (10 mg/kg of body weight every 24 h). Blood PCR was as valuable as splenic aspirate PCR for early diagnosis of acute CME. Splenic aspirate PCR was, however, superior to blood PCR for the evaluation of ehrlichial elimination.

scholarly journals Therapeutic Effect of Doxycycline in Experimental Subclinical Canine Monocytic Ehrlichiosis: Evaluation of a 6-Week Course

1998 ◽  
Vol 36 (7) ◽  
pp. 2140-2142 ◽  
Author(s):  
Shimon Harrus ◽  
Trevor Waner ◽  
Itzhak Aizenberg ◽  
Hylton Bark
Keyword(s):  
Body Weight ◽  
Platelet Count ◽  
Therapeutic Effect ◽  
Carrier State ◽  
Ehrlichia Canis ◽  
Antibody Assay ◽  
Important Indicator ◽  
Doxycycline Treatment ◽  
Canine Monocytic Ehrlichiosis ◽  
Indirect Immunofluorescent

The efficacy of doxycycline treatment (10 mg/kg of body weight every 24 h for 42 days) in eliminating Ehrlichia canisfrom four subclinically infected dogs was evaluated. One dog remained PCR positive, suggesting that 6 weeks of doxycycline treatment may not be sufficient to clear E. canis parasites from all subclinically infected dogs. Serology (indirect immunofluorescent antibody assay) was shown to be unreliable in assessing recovery from the carrier state, as anti-E. canis antibodies persisted after elimination of the parasite. Our findings suggest that an increase in the platelet count may be an important indicator for dogs that recover from subclinical ehrlichiosis.

A Retrospective Study of Dogs Infected with Ehrlichia canis from 2017-2019 in the Thonburi Area of Bangkok Province, Thailand

2020 ◽  
Keyword(s):  
Body Weight ◽  
Retrospective Study ◽  
Liver Enzymes ◽  
Serum Biochemistry ◽  
Ehrlichia Canis ◽  
Housing System ◽  
Housing Systems ◽  
Mixed Breed ◽  
Canine Monocytic Ehrlichiosis ◽  
Open Housing

Ehrlichia canis is a tick-borne rickettsia. It can cause canine monocytic ehrlichiosis (CME). Infected dogs are often reported to have changes in their blood values, such as anemia, thrombocytopenia, increased liver enzymes, and increased kidney function values. This study aimed to collect data that may be related to infected dogs, including age, gender, breed, weight, close-open housing system, the use of ectoparasiticides products. The sample comprised 57 infected dogs. Collecting hematology and serum biochemistry changes in comparison with the reference values of dogs detected with Ehrlichia canis from 2017-2019, Thonburi District, Bangkok, Thailand was also carried out. In summary, dogs infected with Ehrlichia canis mostly included mixed-breed dogs aged between 1 and 10 years. There were no differences in body weight or housing systems. Dogs that had never used ectoparasiticide products or used them intermittently were infected more often (by 7.14 times) than protected. Clinical hematology and serum biochemistry found anemia, thrombocytopenia, and increased liver enzymes.

scholarly journals Efficacy of a Doxycycline Treatment Regimen Initiated during Three Different Phases of Experimental Ehrlichiosis

2010 ◽  
Vol 54 (12) ◽  
pp. 5012-5020 ◽  
Author(s):  
Jennifer C. McClure ◽  
Michelle L. Crothers ◽  
John J. Schaefer ◽  
Patrick D. Stanley ◽  
Glen R. Needham ◽  
...  
Keyword(s):  
Antibiotic Therapy ◽  
Treatment Regimen ◽  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Clinical Parameters ◽  
Blood Samples ◽  
Persistently Infected ◽  
Doxycycline Treatment ◽  
Canine Monocytic Ehrlichiosis ◽  
Three Phases

ABSTRACT Doxycycline is the treatment of choice for canine monocytic ehrlichiosis (CME), a well-characterized disease and valuable model for tick-borne zoonoses. Conflicting reports of clearance of Ehrlichia canis after treatment with doxycycline suggested that the disease phase during which treatment is initiated influences outcomes of these treatments. The purpose of this study was to evaluate the efficacy of a 28-day doxycycline regimen for clearance of experimental E. canis infections from dogs treated during three phases of the disease. Ten dogs were inoculated with blood from E. canis carriers and treated with doxycycline during acute, subclinical, or chronic phases of CME. Daily rectal temperatures and semiweekly blood samples were monitored from each dog, and Rhipicephalus sanguineus ticks were acquisition fed on each dog for xenodiagnosis. Blood collected from dogs treated during acute or subclinical CME became PCR negative for E. canis as clinical parameters improved, but blood samples collected from dogs treated during chronic CME remained intermittently PCR positive. R. sanguineus ticks fed on dogs after doxycycline treatments became PCR positive for E. canis, regardless of when treatment was initiated. However, fewer ticks became PCR positive after feeding on two persistently infected dogs treated with doxycycline followed by rifampin, suggesting that antibiotic therapy can reduce tick acquisition of E. canis.

Strontium 87m Scanning of Pelvic Bones

1968 ◽  
Vol 07 (01) ◽  
pp. 28-36 ◽  
Author(s):  
Władysław Jasiński ◽  
Janina Malinowska ◽  
Henryk Mackiewicz ◽  
Henryk Siwicki ◽  
Krystyna Lukawska
Keyword(s):  
Body Weight ◽  
Differential Diagnosis ◽  
Early Diagnosis ◽  
Femoral Neck ◽  
Early Period ◽  
Early Recurrence ◽  
Physical Activities ◽  
External Irradiation ◽  
Pelvic Bones ◽  
Strontium 87M

SummaryThe purpose of this investigation was to study the accumulation of 87mSr in the proximal parts of the femoral bones of patients treated previously by external irradiation due to cancer of the uterine cervix. It was assumed that this method may be used in the future for the early diagnosis of postirradiation changes of bone (osteoradionecrosis).The incidence of postirradiation changes of the femoral neck among 5735 patients treated between 1950 and 1961 at the Department of Gynaecology of the Institute, was 0.8%. In the early period of postirradiation changes the patients complain only of pain and limitation of physical activities. If radiological and gynaecological findings were negative, the differential diagnosis between early recurrence and early osteoradionecrosis became impossible.49 selected patients were scanned after intravenous injection of 10—115 μCi of 87mSr per kg of body weight (0.5 up to 6.0 mCi). Illustrative cases of normal pelvic bones as well as postirradiation changes are presented and discussed. The authors conclude that the findings justify further systematic studies on the morphology of accumulation of 87mSr in the bones.

Application of Efficient Nanoparticles for Early Diagnosis and Treatment of Cancer

2015 ◽  
Vol 16 (8) ◽  
pp. 662-675 ◽  
Author(s):  
Athanasios Alexiou ◽  
Charalampos Vairaktarakis ◽  
Vasilis Tsiamis ◽  
Ghulam Ashraf
Keyword(s):  
Early Diagnosis ◽  
Diagnosis And Treatment

scholarly journals Small GTPases in Cancer: Still Signaling the Way

Cancers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1500
Author(s):  
Paulo Matos
Keyword(s):  
Early Diagnosis ◽  
Small Gtpases ◽  
Diagnosis And Treatment ◽  
The Way

In recent decades, many advances in the early diagnosis and treatment of cancer have been witnessed [...]

scholarly journals Characterization of the Major Antigenic Protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and Its Application for Serodiagnosis of Ehrlichiosis

2003 ◽  
Vol 10 (4) ◽  
pp. 520-524 ◽  
Author(s):  
Tamece T. Knowles ◽  
A. Rick Alleman ◽  
Heather L. Sorenson ◽  
David C. Marciano ◽  
Edward B. Breitschwerdt ◽  
...  
Keyword(s):  
Blot Analysis ◽  
Single Copy ◽  
Ehrlichia Canis ◽  
Specific Method ◽  
Ehrlichia Chaffeensis ◽  
Antigenic Protein ◽  
Canine Monocytic Ehrlichiosis ◽  
Copy Gene ◽  
Species Specific

ABSTRACT Canine monocytic ehrlichiosis, caused by Ehrlichia canis or Ehrlichia chaffeensis, can result in clinical disease in naturally infected animals. Coinfections with these agents may be common in certain areas of endemicity. Currently, a species-specific method for serological diagnosis of monocytic ehrlichiosis is not available. Previously, we developed two indirect enzyme-linked immunosorbent assays (ELISAs) using the major antigenic protein 2 (MAP2) of E. chaffeensis and E. canis. In this study, we further characterized the conservation of MAP2 among various geographic isolates of each organism and determined if the recombinant MAP2 (rMAP2) of E. chaffeensis would cross-react with E. canis-infected dog sera. Genomic Southern blot analysis using digoxigenin-labeled species-specific probes suggested that map2 is a single-copy gene in both Ehrlichia species. Sequences of the single map2 genes of seven geographically different isolates of E. chaffeensis and five isolates of E. canis are highly conserved among the various isolates of each respective ehrlichial species. ELISA and Western blot analysis confirmed that the E. chaffeensis rMAP2 failed to serologically differentiate between E. canis and E. chaffeensis infections.

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