scholarly journals Penetration of Piperacillin and Tazobactam into Inflamed Soft Tissue of Patients with Diabetic Foot Infection

2005 ◽  
Vol 49 (10) ◽  
pp. 4368-4371 ◽  
Author(s):  
F. J. Legat ◽  
R. Krause ◽  
P. Zenahlik ◽  
C. Hoffmann ◽  
S. Scholz ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Diabetic Foot ◽  
Extracellular Space ◽  
Soft Tissues ◽  
In Vivo Microdialysis ◽  
Diabetic Foot Infection

ABSTRACT We investigated the pharmacokinetics of piperacillin and tazobactam in the extracellular space fluid of inflamed soft tissues of six patients with diabetic foot infection using in vivo microdialysis and found similar penetration for piperacillin but not for tazobactam into inflamed and noninflamed soft tissue.

scholarly journals Penetration of Fosfomycin into Inflammatory Lesions in Patients with Cellulitis or Diabetic Foot Syndrome

2003 ◽  
Vol 47 (1) ◽  
pp. 371-374 ◽  
Author(s):  
F. J. Legat ◽  
A. Maier ◽  
P. Dittrich ◽  
P. Zenahlik ◽  
T. Kern ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Broad Spectrum ◽  
Diabetic Foot ◽  
Lower Extremities ◽  
In Vivo Microdialysis ◽  
Diabetic Foot Syndrome ◽  
Broad Spectrum Antibiotic ◽  
Inflammatory Lesions ◽  
Diabetic Foot Infection

ABSTRACT We investigated the distribution of the broad-spectrum antibiotic fosfomycin in infected soft tissue of patients with uncomplicated cellulitis of the lower extremities or diabetic foot infection using in vivo microdialysis. Our findings suggest that fosfomycin exhibits good and similar penetration into the fluid in the interstitial space in inflamed and noninflamed soft tissue in patients.

scholarly journals In Vivo Microdialysis Study of the Penetration of Daptomycin into Soft Tissues in Diabetic versus Healthy Volunteers

2008 ◽  
Vol 52 (11) ◽  
pp. 3941-3946 ◽  
Author(s):  
Aryun Kim ◽  
Larry A. Suecof ◽  
Christina A. Sutherland ◽  
Lihong Gao ◽  
Joseph L. Kuti ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Healthy Volunteers ◽  
Healthy Subjects ◽  
Soft Tissues ◽  
In Vivo Microdialysis ◽  
Free Drug ◽  
Target Tissue ◽  
Drug Concentrations ◽  
Complicated Skin

ABSTRACT Daptomycin is approved for the treatment of complicated skin and soft tissue infections, including diabetic wounds of the lower extremities, at a dose of 4 mg/kg of body weight once daily. For such localized tissue infections, drug concentrations in the interstitial space are an important determinant of successful therapy. In the diabetic population, peripheral arterial disease may limit antibiotic penetration into the target tissue. The objective of this study was to describe and compare the pharmacokinetic profiles of daptomycin in the interstitial fluid of soft tissues in diabetic and healthy volunteers by using in vivo microdialysis. Twelve subjects (six diabetic and six healthy) received a single 4-mg/kg dose of daptomycin intravenously. Samples of plasma and tissue were simultaneously collected over 24 h. Diabetic and healthy groups were matched in mean age (±10 years), gender ratio, mean weight (±10 kg), and creatinine clearance rate (±20 ml/min/1.73 m2). Pharmacokinetic parameters for plasma were similar between groups (P > 0.05). The mean peak drug concentrations ± standard deviations in tissue were 4.3 ± 3.3 μg/ml and 3.8 ± 1.4 μg/ml for diabetic and healthy subjects, respectively. The degree of tissue penetration, defined as the ratio of the area under the free drug concentration-time curve for tissue to that for plasma, was 0.93 ± 0.61 for diabetic subjects and 0.74 ± 0.09 for healthy subjects (P = 0.46). Daptomycin at 4 mg/kg penetrated well into the soft tissue, reaching concentrations approximately 70 to 90% of those of the free drug in plasma. Moreover, these free, bioactive concentrations in tissue exceeded the MICs for staphylococci and streptococci over the 24-h dosing interval.

scholarly journals In vivo soft tissue reinforcement with bacterial nanocellulose

2021 ◽  
Author(s):  
Irene Anton-Sales ◽  
Soledad Roig-Sanchez ◽  
Kamelia Anna Traeger ◽  
Christine Weis ◽  
Anna Laromaine ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Soft Tissues ◽  
Hernia Surgery ◽  
Bacterial Nanocellulose ◽  
Surgical Meshes

The use of surgical meshes to reinforce damaged internal soft tissues has been instrumental for successful hernia surgery; a highly prevalent condition affecting yearly more than 20 million patients worldwide....

Reliability Investigation of Tissue Resonator Indenter Device

2010 ◽  
Author(s):  
Ming Jia ◽  
Jean W. Zu ◽  
Alireza Hariri
Keyword(s):  
Mechanical Properties ◽  
Soft Tissue ◽  
Soft Tissues ◽  
Tissue Properties ◽  
University Of Toronto ◽  
In Vivo Measurements ◽  
Disease Diagnostics ◽  
Working Principle ◽  
The University

Knowledge of tissue mechanical properties is widely required by medical applications, such as disease diagnostics, surgery operation, simulation, planning, and training. A new portable device, called Tissue Resonator Indenter Device (TRID), has been developed for measurement of regional viscoelastic properties of soft tissues at the Bio-instrument and Biomechanics Lab of the University of Toronto. As a device for soft tissue properties in-vivo measurements, the reliability of TRID is crucial. This paper presents TRID’s working principle and the experimental study of TRID’s reliability with respect to inter-reliability, intra-reliability, and the indenter misalignment effect as well. The experimental results show that TRID is a reliable device for in-vivo measurements of soft tissue mechanical properties.

Using MR Imaging and X-Ray Fluoroscopy to Quantify the Effects of Soft-Tissue Artifact on Measurement of Knee-Joint Kinematics

2009 ◽  
Author(s):  
Massoud Akbarshahi ◽  
Justin W. Fernandez ◽  
Anthony Schache ◽  
Richard Baker ◽  
Marcus G. Pandy
Keyword(s):  
Soft Tissue ◽  
Knee Joint ◽  
Soft Tissues ◽  
Surgical Techniques ◽  
Joint Kinematics ◽  
Segmental Motion ◽  
Coordinate Systems ◽  
Knee Joint Kinematics ◽  
Soft Tissue Artifact

The ability to accurately measure joint kinematics in vivo is of critical importance to researchers in the field of biomechanics [1]. Applications range from the quantitative evaluation of different surgical techniques, treatment methods and/or implant designs, to the development of computer-based models capable of simulating normal and pathological musculoskeletal conditions [1,2]. Currently, non-invasive marker-based three dimensional (3D) motion analysis is the most commonly used method for quantitative assessment of normal and pathological locomotion. The accuracy of this technique is influenced by movement of the soft tissues relative to the underlying bones, which causes inaccuracies in the determination of segmental anatomical coordinate systems and tracking of segmental motion. The purpose of this study was to quantify the errors in the measurement of knee-joint kinematics due solely to soft-tissue artifact (STA) in healthy subjects. To facilitate valid inter-subject comparisons of the kinematic data, relevant anatomical coordinate systems were defined using 3D bone models generated from magnetic resonance imaging (MRI).

Antibiotic Resistance in Diabetic Foot Soft Tissue Infections: A Series From Greece

2017 ◽  
Vol 16 (4) ◽  
pp. 255-259 ◽  
Author(s):  
Maria Demetriou ◽  
Nikolaos Papanas ◽  
Periklis Panagopoulos ◽  
Maria Panopoulou ◽  
Efstratios Maltezos
Keyword(s):  
Antibiotic Resistance ◽  
Soft Tissue ◽  
Diabetic Foot ◽  
Soft Tissues ◽  
Foot Ulcers ◽  
Diabetic Foot Ulcers ◽  
Soft Tissue Infections ◽  
Diabetic Foot Infections ◽  
Initial Choice ◽  
Gram Positive Cocci

Diabetic foot infections are a common and serious problem for all health systems worldwide. The aim of this study was to examine the resistance to antibiotics of microorganisms isolated from infected soft tissues of diabetic foot ulcers, using tissue cultures. We included 113 consecutive patients (70 men, 43 women) with a mean age of 66.4 ± 11.2 years and a mean diabetes duration of 14.4 ± 7.6 years presenting with diabetic foot soft tissue infections. Generally, no high antibiotic resistance was observed. Piperacillin-tazobactam exhibited the lowest resistance in Pseudomonas, as well as in the other Gram-negative pathogens. In methicillin-resistant Staphylococcus aureus isolates, there was no resistance to anti-Staphylococcus agents. Of note, clindamycin, erythromycin, and amoxycillin/clavulanic acid exhibited high resistance in Gram-positive cocci. These results suggest that antibiotic resistance in infected diabetic foot ulcers in our area is not high and they are anticipated to prove potentially useful in the initial choice of antibiotic regimen.

scholarly journals Penetration of Linezolid into Soft Tissues of Healthy Volunteers after Single and Multiple Doses

2005 ◽  
Vol 49 (6) ◽  
pp. 2367-2371 ◽  
Author(s):  
Pejman Dehghanyar ◽  
Cornelia Bürger ◽  
Markus Zeitlinger ◽  
Florian Islinger ◽  
Florian Kovar ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Soft Tissues ◽  
In Vivo Microdialysis ◽  
Time Curve ◽  
Concentration Time ◽  
Multiple Doses ◽  
Muscle Tissues ◽  
Subcutaneous Adipose ◽  
Free Plasma

ABSTRACT The present study tested the ability of linezolid to penetrate soft tissues in healthy volunteers. Ten healthy volunteers were subjected to linezolid drug intake at a dose of 600 mg twice a day for 3 to 5 days. The first dose was administered intravenously. All following doses were self-administered orally. The tissue penetration of linezolid was assessed by use of in vivo microdialysis. In the single-dose experiments the ratios of the area under the concentration-time curve from 0 to 8 h (AUC0-8) for tissue to the AUC0-8 for free plasma were 1.4 ± 0.3 (mean ± standard deviation) and 1.3 ± 0.4 for subcutaneous adipose and muscle tissue, respectively. After multiple doses, the corresponding mean ratios were 0.9 ± 0.2 and 1.0 ± 0.5, respectively. The ratios of the AUC from 0 to 24 h (AUC0-24) for free linezolid in tissues to the MIC were between 50 and 100 for target pathogens with MICs between 2 and 4 mg/liter. In conclusion, the present study showed that linezolid penetrates rapidly into the interstitial space fluid of subcutaneous adipose and skeletal muscle tissues in healthy volunteers. On the basis of pharmacokinetic-pharmacodynamic calculations, we suggest that linezolid concentrations in soft tissues can be considered sufficient to inhibit the growth of many clinically relevant bacteria.

scholarly journals Distribution and Antimicrobial Activity of Fosfomycin in the Interstitial Fluid of Human Soft Tissues

2000 ◽  
Vol 44 (10) ◽  
pp. 2728-2732 ◽  
Author(s):  
Martin Frossard ◽  
Christian Joukhadar ◽  
Boban M. Erovic ◽  
Peter Dittrich ◽  
Paulus E. Mrass ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Soft Tissues ◽  
Pharmacokinetic Profile ◽  
Interstitial Space ◽  
Soft Tissue Infections ◽  
Time Curve ◽  
Tract Infections ◽  
High Degree

ABSTRACT Fosfomycin is a broad-spectrum antibiotic which is established as therapy for uncomplicated lower urinary tract infections. In addition, preliminary data indicate that fosfomycin has a potential role in the treatment of soft tissue infections. However, the use of fosfomycin has not been established for this condition, and it is unclear whether the level of fosfomycin penetration into human soft tissues is high enough to eradicate relevant pathogens. To better characterize the antibiotic potential of fosfomycin, we applied a combined in vivo pharmacokinetic-in vitro pharmacodynamic model to human volunteers. For this purpose fosfomycin concentrations in vivo in the fluid of the interstitial space of human soft tissues were measured by microdialysis following intravenous infusion of 4 or 8 g of fosfomycin (n = 6). Subsequently, bacterial isolates with relevance for soft tissue infections were exposed to concentrations according to the in vivo pharmacokinetic profile in the interstitial space fluid obtained by microdialysis. Our experiments indicated a high degree of soft tissue penetration for fosfomycin, with ratios of the area under the concentration-time curve from 0 to 8 h for muscle (AUC0–8muscle )/AUC0–8serum of 0.48 ± 0.08 and 0.53 ± 0.04 and ratios of AUC0–8adipose tissue /AUC0–8serum of 0.74 ± 0.12 and 0.71 ± 0.11 following administration of 4 and 8 g, respectively. In corresponding in vitro simulation experiments with selected isolates of Staphylococcus aureus,Enterobacter cloacae, and Serratia marcescensfor which MICs were 16 μg/ml, organisms were undetectable after a single dosing interval. Fosfomycin exhibits a strong ability to penetrate into the fluid of the interstitial space of soft tissues and reaches levels sufficient to substantially inhibit the growth of relevant bacteria at the target site. We therefore conclude that fosfomycin might qualify as an alternative candidate for the therapy of soft tissue infections.

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