scholarly journals Epoxyalkane:Coenzyme M Transferase Gene Diversity and Distribution in Groundwater Samples from Chlorinated-Ethene-Contaminated Sites

2016 ◽  
Vol 82 (11) ◽  
pp. 3269-3279 ◽  
Author(s):  
Xikun Liu ◽  
Timothy E. Mattes
Keyword(s):  
Gene Diversity ◽  
Critical Role ◽  
Aerobic Biodegradation ◽  
Contaminated Sites ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Future Research ◽  
Content Type ◽  
Groundwater Samples ◽  
Degrading Bacteria

ABSTRACTEpoxyalkane:coenzyme M transferase (EaCoMT) plays a critical role in the aerobic biodegradation and assimilation of alkenes, including ethene, propene, and the toxic chloroethene vinyl chloride (VC). To improve our understanding of the diversity and distribution of EaCoMT genes in the environment, novel EaCoMT-specific terminal-restriction fragment length polymorphism (T-RFLP) and nested-PCR methods were developed and applied to groundwater samples from six different contaminated sites. T-RFLP analysis revealed 192 different EaCoMT T-RFs. Using clone libraries, we retrieved 139 EaCoMT gene sequences from these samples. Phylogenetic analysis revealed that a majority of the sequences (78.4%) grouped with EaCoMT genes found in VC- and ethene-assimilatingMycobacteriumstrains andNocardioidessp. strain JS614. The four most-abundant T-RFs were also matched with EaCoMT clone sequences related toMycobacteriumandNocardioidesstrains. The remaining EaCoMT sequences clustered within two emergent EaCoMT gene subgroups represented by sequences found in propene-assimilatingGordonia rubripertinctastrain B-276 andXanthobacter autotrophicusstrain Py2. EaCoMT gene abundance was positively correlated with VC and ethene concentrations at the sites studied.IMPORTANCEThe EaCoMT gene plays a critical role in assimilation of short-chain alkenes, such as ethene, VC, and propene. An improved understanding of EaCoMT gene diversity and distribution is significant to the field of bioremediation in several ways. The expansion of the EaCoMT gene database and identification of incorrectly annotated EaCoMT genes currently in the database will facilitate improved design of environmental molecular diagnostic tools and high-throughput sequencing approaches for future bioremediation studies. Our results further suggest that potentially significant aerobic VC degraders in the environment are not well represented in pure culture. Future research should aim to isolate and characterize aerobic VC-degrading bacteria from these underrepresented groups.

Accessing resources for service innovation – the critical role of network relationships

2014 ◽  
Vol 25 (1) ◽  
pp. 2-29 ◽  
Author(s):  
Helena Rusanen ◽  
Aino Halinen ◽  
Elina Jaakkola
Keyword(s):  
Critical Role ◽  
Service Innovation ◽  
Innovation Process ◽  
Research Strategy ◽  
Future Research ◽  
Theoretical Understanding ◽  
Resource Access ◽  
Content Type ◽  
External Resources ◽  
Different Types

Purpose – This paper aims to explore how companies access resources through network relationships when developing service innovations. The paper identifies the types of resource that companies seek from other actors and examines the nature of relationships and resource access strategies that can be applied to access each type of resource. Design/methodology/approach – A longitudinal, multi-case study is conducted in the field of technical business-to-business (b-to-b) services. An abductive research strategy is applied to create a new theoretical understanding of resource access. Findings – Companies seek a range of resources through different types of network relationships for service innovation. Four types of resource access strategies were identified: absorption, acquisition, sharing, and co-creation. The findings show how easily transferable resources can be accessed through weak relationships and low-intensity collaboration. Access to resources that are difficult to transfer, instead, necessitates strong relationships and high-intensity collaboration. Research limitations/implications – The findings are valid for technical b-to-b services, but should also be tested for other kinds of innovations. Future research should also study how actors integrate the resources gained through networks in the innovation process. Practical implications – Managers should note that key resources for service innovation may be accessible through a variety of actors and relationships ranging from formal arrangements to miscellaneous social contacts. To make use of tacit resources such as knowledge, firms need to engage in intensive collaboration. Originality/value – Despite attention paid to network relationships, innovation collaboration, and external resources, previous research has neither linked these issues nor studied their mutual contingencies. This paper provides a theoretical model that characterizes the service innovation resources accessible through different types of relationships and access strategies.

Healthcare supply chain management: literature review and some issues

2018 ◽  
Vol 15 (3) ◽  
pp. 265-287 ◽  
Author(s):  
Bhavana Mathur ◽  
Sumit Gupta ◽  
Makhan Lal Meena ◽  
G.S. Dangayach
Keyword(s):  
Supply Chain ◽  
Supply Chain Management ◽  
Literature Review ◽  
Organizational Performance ◽  
Critical Role ◽  
Sample Survey ◽  
Future Research ◽  
Chain Management ◽  
Content Type ◽  
Potential Benefits

PurposeThe purpose of this paper is to examine the causal linkages among supply chain practices, effectiveness of supply chain performance (SCP) and organizational performance (OP) in Indian healthcare industries.Design/methodology/approachThis paper is helpful in developing a framework for linking a healthcare supply chain practice to its OP, and thus identifies how such a linkage can be connected to the effectiveness of SCP. Such effort also enables the authors to derive a set of recommended supply chain practices for SC performance.FindingsFrom the literature review, this paper finds that, in the context of Indian healthcare industries, efficient SC performance may play a critical role for overall OP improvement, as there is a close interrelationship between supply chain management (SCM) practices and SCP that may have a more significant effect on OP improvement.Research limitations/implicationsThe principle limitation of the paper is that it is performed only in a particular industry and with a questionnaire survey which could be extended in future for other industries also. Another limitation of the paper is that it is focused only on the SCP of medical device and equipment supply chain which is a small portion of the whole healthcare supply chain, and therefore requires further research covering various other domains of healthcare supply chain. Another limitation of the study is that the sample survey has been taken from only one respondent per company at one point of time which may create biasness in the results. Thus, future research should collect data through multiple members from the organization.Practical implicationsThis study contributes to know the effect of SCM practices on healthcare SCP and provides a practical and useful tool to evaluate the extent of effectiveness of SCP and finally their impact on the healthcare OP. Finally, this study provides conceptual and descriptive literature regarding SCM practices that leads to improvement in healthcare performance.Social implicationsThis study adds to the knowledge on healthcare SCM performance by exploring the relationship between supply chain practices, healthcare SCP and healthcare OP and by developing and testing a research framework thus help in improving patient satisfaction.Originality/valueThis study attempts to show how the potential benefits of supply chain practices can no longer be ignored in healthcare supply chain.

Post-occupancy evaluation of outdoor spaces on the campus of the Federal University of Juiz de Fora, Brazil

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Ana Clara Carvalho Tourinho ◽  
Sabrina Andrade Barbosa ◽  
Özgür Göçer ◽  
Klaus Chaves Alberto
Keyword(s):  
Critical Role ◽  
Future Research ◽  
Content Type ◽  
University Campuses ◽  
Post Occupancy Evaluation ◽  
Outdoor Spaces ◽  
The University ◽  
The Impact ◽  
Practical Implications

PurposeUsing the campus of a Brazilian university as case study, this research aims to identify which aspects of the outdoor spaces are the most significant in attracting people.Design/methodology/approachThis research relies on the application of different post-occupancy evaluation (POE) methods, including user tracking, behavioural mapping and questionnaires, on one plateau of the campus.FindingsThree group of aspects (socialization, proximity and infrastructure) were identified as key elements in explaining the impact of the campus physical characteristics on users’ behaviour. The results indicate that having characteristics of at least one group of aspects in those spaces can guarantee their vitality and, if there is presence of attributes of more than one group, liveliness can be increased.Research limitations/implicationsFurther studies should be conducted on an entire campus to identify other spatial elements in the three groups.Practical implicationsThis research contributes to the planning of future campuses and to solutions to the existed ones, indicating the most relevant spatial characteristics to be considered. Additionally, the combination of different methods may be useful to future research.Originality/valueMost of the investigations on the university campuses focus on the buildings, and little research has investigated the outdoor spaces, although they play a critical role in learning and academic life, where people establish social, cultural and personal relationships. In addition, studies using several POE allowed a consistent and complete diagnostic about the aspects of the campus, giving recommendations for future projects.

Gamblers’ aces in the hole: the effect of erroneous cognitions on dysfunctional gambling

2016 ◽  
Vol 6 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Donata Tania Vergura
Keyword(s):  
Problem Gambling ◽  
Structural Equation ◽  
Online Survey ◽  
Negative Relationship ◽  
Severity Index ◽  
Diagnostic Tools ◽  
Future Research ◽  
Gambling Behaviour ◽  
Content Type ◽  
Gambling Involvement

Purpose – The purpose of this paper is to investigate whether gambler’s cognitive errors affect gambling involvement and addiction. As the popularity of gambling has grown, questions are being raised about its excessive use and factors related to addictive behaviours. Design/methodology/approach – An online survey on 508 gamblers was conducted. Structural equation modelling was used to investigate the relationships among gambling beliefs, gambling involvement and problem gambling. Findings – Among the three sets of erroneous beliefs investigated, luck and superstition were significant predictors of both gambling involvement and the severity of gambling problems (according to the Problem Gambling Severity Index (PGSI)), while the illusion of control showed a negative relationship with the PGSI. Moreover, gambling involvement positively affected the potential risk of disease and mediates the relationship between luck and superstition and PGSI. Research limitations/implications – Because not all cognitive dimensions apparently influence gambling behaviour, future research should extend the analysis to include other variables that may moderate or mediate the causal relationship. Practical implications – The results are useful to marketers in developing social marketing campaigns wishing to discourage gambling. Moreover, factors that influence gambling involvement and addiction may be used as diagnostic tools to correct gamblers behaviour. Originality/value – The paper proposes a deeper exploration of the relationships among beliefs, gambling involvement and dysfunctional gambling and an appropriate scale to capture the entire spectrum of gambler’s beliefs in relation to every form of gambling activity.

scholarly journals Aggressive Emerging Pathovars of Xanthomonas arboricola Represent Widespread Epidemic Clones Distinct from Poorly Pathogenic Strains, as Revealed by Multilocus Sequence Typing

2015 ◽  
Vol 81 (14) ◽  
pp. 4651-4668 ◽  
Author(s):  
Marion Fischer-Le Saux ◽  
Sophie Bonneau ◽  
Salwa Essakhi ◽  
Charles Manceau ◽  
Marie-Agnès Jacques
Keyword(s):  
Genetic Structure ◽  
Xanthomonas Campestris ◽  
Plant Pathogens ◽  
Genetic Relationships ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Phylogenic Tree ◽  
Content Type ◽  
Epidemiological Surveys ◽  
Xanthomonas Arboricola

ABSTRACTDeep and comprehensive knowledge of the genetic structure of pathogenic species is the cornerstone on which the design of precise molecular diagnostic tools is built.Xanthomonas arboricolais divided into pathovars, some of which are classified as quarantine organisms in many countries and are responsible for diseases on nut and stone fruit trees that have emerged worldwide. Recent taxonomic studies of the genusXanthomonasshowed that strains isolated from other hosts should be classified inX. arboricola, extending the host range of the species. To investigate the genetic structure ofX. arboricolaand the genetic relationships between highly pathogenic strains and strains apparently not relevant to plant health, we conducted multilocus sequence analyses on a collection of strains representative of the known diversity of the species. Most of the pathovars were clustered in separate monophyletic groups. The pathovars pruni, corylina, and juglandis, responsible for pandemics in specific hosts, were highly phylogenetically related and clustered in three distinct clonal complexes. In contrast, strains with no or uncertain pathogenicity were represented by numerous unrelated singletons scattered in the phylogenic tree. Depending on the pathovar, intra- and interspecies recombination played contrasting roles in generating nucleotide polymorphism. This work provides a population genetics framework for molecular epidemiological surveys of emerging plant pathogens withinX. arboricola. Based on our results, we propose to reclassify three former pathovars ofXanthomonas campestrisasX. arboricolapv. arracaciae comb. nov.,X. arboricolapv. guizotiae comb. nov., andX. arboricolapv. zantedeschiae comb. nov. An emended description ofX. arboricolaVauterin et al. 1995 is provided.

An examination of ecopreneurs’ incentives through a combination between institutional and resource-based approach

2018 ◽  
Vol 29 (2) ◽  
pp. 195-215 ◽  
Author(s):  
Ioannis E. Nikolaou ◽  
Konstantinos P. Tsagarakis ◽  
Kyriaki Tasopoulou
Keyword(s):  
Environmental Sustainability ◽  
Service Sector ◽  
Research Question ◽  
Critical Role ◽  
Sample Selection ◽  
Future Research ◽  
Key Factors ◽  
Policy Makers ◽  
Content Type ◽  
Selection Of

Purpose The purpose of this paper is to address two research questions: which are the key factors that stimulate entrepreneurs to invest in ecopreneurship, and how ecopreneurhsip contributes to environmental sustainability. Design/methodology/approach To answer these questions, a framework has been developed to identify the incentives that lead entrepreneurs to invest in firms in the ecopreneurship through institutional and resource-based thinking. Findings From a survey of 91 Greek firms from the green service sector, it is shown that some specific institutional and resource-based view factors play a critical role in green entrepreneurs’ decisions, as well as some certain environmental practices that are frequently used by entrepreneurs to address environmental issues. Research limitations/implications First, the answer of the second research question through data collected by a questionnaire survey may be faced with skepticism by some authors, as it could be seen that entrepreneurs and managers of firms could have overstated their company's environmental activities. Second, although the sample selection of 91 firms is a representative sample (response rate 12.35 percent) of the total population of Greek green firms (761) and equal to other relative studies, a higher number of firms and a wider variety of green entrepreneurship ventures is necessary in future research. Practical implications The findings are useful for scholars, practitioners and policy makers since it provide information regarding the behavior of green entrepreneurs. Originality/value The paper analyze the types of green entrepreneurs in relation to the different features and strategies which are emerged from two theories, such as institutional and resource-based theory.

scholarly journals Biobanking for cancer research: Preservation of tissue integrity – Some technical considerations

2012 ◽  
Vol 01 (02) ◽  
pp. 130-138 ◽  
Author(s):  
Anita Mahadevan ◽  
S.K. Shankar
Keyword(s):  
Biomarker Discovery ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Future Research ◽  
Pathology Laboratory ◽  
Tissue Integrity ◽  
Oncology Research ◽  
Genomic Studies ◽  
And Storage

Abstract Biobanking and biomarker discovery have become an integral part of neuro-oncology research. Towards achieving this end, the essential requirement is optimizing methods of tissue preservation of human tissues removed at surgery for diagnostic purposes and banking them for subserving future research. Owing to recent advances in molecular diagnostic tools, this clinical material has become a precious source for proteomic and genomic studies. The advent of biotechnological tools such as microarray, proteomics, and genomics has made it essential to preserve not just morphology but also the quality of nucleic acids and proteins, changing the traditional workflow of a pathology laboratory. It is therefore essential to develop simple technologies for tissue fixation and storage ensure that receptor and molecular integrity is reasonably maintained. Knowledge of the basic chemistry of tissue fixatives, the biochemical changes that take place in biological material by utilizing different techniques of fixation is essential while undertaking molecular, genomic, and proteomic studies on fresh and archival tissues.

scholarly journals Job insecurity and performance over time: the critical role of job insecurity duration

2019 ◽  
Vol 25 (3) ◽  
pp. 325-336 ◽  
Author(s):  
Maike E. Debus ◽  
Dana Unger ◽  
Cornelius J. König
Keyword(s):  
Job Performance ◽  
Job Insecurity ◽  
Critical Role ◽  
Future Research ◽  
Appraisal Theory ◽  
Stress Reactions ◽  
Content Type ◽  
Dynamic Perspective ◽  
Over Time

Purpose Research on the relationship between job insecurity and job performance has thus far yielded inconclusive results. The purpose of this paper is to offer a more dynamic perspective on the effects of job insecurity on job performance. Design/methodology/approach Drawing from cognitive appraisal theory, research on critical life events, and stress reactions as well as more general theorizing around the role of time, this paper proposes that individuals’ job performance reactions to job insecurity will be dynamic over time. Findings Adopting a person-centered perspective, this paper suggests that there are seven subpopulations that differ in their intra-individual job performance change patterns over time. Research limitations/implications This paper presents potential predictors of subpopulation membership and presents an agenda for future research. Originality/value We contribute to the literature by introducing a dynamic perspective to the study of job performance in the context of job insecurity. Delineating a set of open questions that follow from the presented theoretical arguments, the authors also hope to stimulate future research in the context of job insecurity and job performance.

Metaphors we manage and develop quality by

2020 ◽  
Vol 12 (4) ◽  
pp. 405-416
Author(s):  
Johan Lilja ◽  
Pernilla Ingelsson ◽  
Kristen Snyder ◽  
Ingela Bäckström ◽  
Christer Hedlund
Keyword(s):  
Quality Management ◽  
Literature Review ◽  
Design Methodology ◽  
Dynamic Properties ◽  
Critical Role ◽  
Critical Discussion ◽  
Future Research ◽  
Content Type ◽  
Selection Of

Purpose Metaphors are a powerful and human way of understanding and experiencing one kind of thing in terms of another. In quality management (QM), several metaphors are used to describe and bring to life the often-abstract QM concepts and systems. These metaphors are of great importance for how QM is understood, communicated and practiced. However, the metaphors of QM have seldom been systematically screened or put in focus, neither the topic of a critical discussion. The purpose of this paper is hence to contribute with a screening of the metaphors currently used, within QM literature and in practice among QM leaders, and then elaborate on their potential for improvement and development. Design/methodology/approach The paper is based on a literature review combined with interviews of QM leaders. Findings The paper highlights that the current QM metaphors provide intuitive associations to properties such as stability, shelter, and structure, but not to the important dynamic properties of QM, such as learning, or to the critical role of people in QM. What can be seen as core properties of QM are communicated by texts or labels added on to metaphors with properties that often are in sharp contrast to them. The paper also provides suggestions for further improvements and development. Originality/value The paper highlights the area of metaphors within QM as an important area for future research. It also provides insights concerning the successful use and selection of metaphors in future QM practice.

Principals' intentions and anticipated challenges in implementing nutrition education

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Fozia Ahmed Baloch ◽  
Shaik Abdul Malik Mohamed Ismail ◽  
Nazir Ahmed Jogezai
Keyword(s):  
Secondary School ◽  
Nutrition Education ◽  
School Principals ◽  
Secondary School Principals ◽  
Critical Role ◽  
Future Research ◽  
Content Type ◽  
Roles And Responsibilities ◽  
Mixed Method Design

PurposeThis study aimed to know principals' intentions of implementing nutrition education (NE) and explore the challenges they may anticipate while implementing NE in their respective schools.Design/methodology/approachThe study employed a sequential mixed-method design using a questionnaire and interviews to collect data. The quantitative data were collected from 378 secondary school principals, while 16 school principals were interviewed.FindingsThe study found principals with positive intentions towards implementing NE. The results also highlighted challenges related to capacity building, resources (both human and physical), policies and plans, roles, and responsibilities of the implementers (teachers and principals), support from higher authorities, community participation and teachers' unions. This study concludes that principals' intentions remain central to the implementation of NE in schools. However, it is necessary to overcome those challenges before its implementation.Research limitations/implicationsThe study sought to ascertain principals' intentions rather than their actual behaviour of NE implementation and hence remains limited in this area, which future research may consider. Furthermore, the research is limited to the principals' opinions on the anticipated challenges associated with NE implementation. The study did not solicit the opinions of other stakeholders, such as education managers, policymakers, teachers and communities.Originality/valueThe article is significant in terms of NE being implemented in schools to improve students' physical and academic wellbeing. The critical role of principals has been investigated by determining their intentions and the anticipated challenges associated with implementing NE. The authors declare the originality of the data.

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