Random Insertion of a TetR-Inducing Peptide Tag into Escherichia coli Proteins Allows Analysis of Protein Levels by Induction of Reporter Gene Expression

ABSTRACT The insertion element InsTipα was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.

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Serpent/dGATAb regulates Laminin B1 and Laminin B2 expression during Drosophila embryogenesis

Scientific Reports ◽

10.1038/s41598-019-52210-9 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Uwe Töpfer ◽

Maik C. Bischoff ◽

Marek Bartkuhn ◽

Anne Holz

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Transcriptional Regulation ◽

Reporter Gene ◽

Expression Patterns ◽

Reporter Gene Expression ◽

Drosophila Embryogenesis ◽

Gata Transcription Factor ◽

Important Regulator ◽

Direct Binding

Abstract Transcriptional regulation of Laminin expression during embryogenesis is a key step required for proper ECM assembly. We show, that in Drosophila the Laminin B1 and Laminin B2 genes share expression patterns in mesodermal cells as well as in endodermal and ectodermal gut primordia, yolk and amnioserosa. In the absence of the GATA transcription factor Serpent, the spatial extend of Laminin reporter gene expression was strongly limited, indicating that Laminin expression in many tissues depends on Serpent activity. We demonstrate a direct binding of Serpent to the intronic enhancers of Laminin B1 and Laminin B2. In addition, ectopically expressed Serpent activated enhancer elements of Laminin B1 and Laminin B2. Our results reveal Serpent as an important regulator of Laminin expression across tissues.

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Single Cell Quantification of Reporter Gene Expression in Live Adult Caenorhabditis elegans Reveals Reproducible Cell-Specific Expression Patterns and Underlying Biological Variation

PLoS ONE ◽

10.1371/journal.pone.0124289 ◽

2015 ◽

Vol 10 (5) ◽

pp. e0124289 ◽

Cited By ~ 23

Author(s):

Alexander R. Mendenhall ◽

Patricia M. Tedesco ◽

Bryan Sands ◽

Thomas E. Johnson ◽

Roger Brent

Keyword(s):

Gene Expression ◽

Caenorhabditis Elegans ◽

Single Cell ◽

Reporter Gene ◽

Expression Patterns ◽

Biological Variation ◽

Reporter Gene Expression ◽

Specific Expression ◽

Cell Quantification

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Two Novel Adenovirus Vector Systems Permitting Regulated Protein Expression in Gene Transfer Experiments

Journal of Virology ◽

10.1128/jvi.72.10.8358-8361.1998 ◽

1998 ◽

Vol 72 (10) ◽

pp. 8358-8361 ◽

Cited By ~ 48

Author(s):

Magnus Molin ◽

Maria C. Shoshan ◽

Karin Öhman-Forslund ◽

Stig Linder ◽

Göran Akusjärvi

Keyword(s):

Gene Expression ◽

Protein Expression ◽

Reporter Gene ◽

Adenovirus Vector ◽

Cell Types ◽

Reporter Gene Expression ◽

Reporter Protein ◽

Ru 486 ◽

Vector Systems ◽

Wide Range

ABSTRACT Two new adenovirus vector systems based on the tetracycline-regulated Tet-ON- (Gossen, M., et al., Science 268:1766–1769, 1995) and the RU 486-regulated progesterone antagonist (Wang, Y., et al., Proc. Natl. Acad. Sci. USA 91:8180–8184, 1994)-induced gene expression systems are described. We show that both systems permit a tight control of chloramphenicol acetyltransferase reporter gene expression in a variety of cell types, with induction levels of approximately 1,800-fold (Tet-ON system) and 600-fold (RU 486-regulated system), respectively. A significant advantage of our vector systems is that reporter protein expression can be adjusted over a wide range by varying the amount of inducer. The Tet-ON system is also shown to permit an efficient control of reporter gene expression in mice.

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