scholarly journals High-Level Diversity of Dinoflagellates in the Natural Environment, Revealed by Assessment of Mitochondrial cox1 and cob Genes for Dinoflagellate DNA Barcoding

2009 ◽  
Vol 75 (12) ◽  
pp. 4230-4230 ◽  
Author(s):  
Senjie Lin ◽  
Huan Zhang ◽  
Yubo Hou ◽  
Yunyun Zhuang ◽  
Lilibeth Miranda
Keyword(s):  
Dna Barcoding ◽  
Natural Environment ◽  
Mitochondrial Cox1 ◽  
High Level

scholarly journals High-Level Diversity of Dinoflagellates in the Natural Environment, Revealed by Assessment of Mitochondrial cox1 and cob Genes for Dinoflagellate DNA Barcoding

2008 ◽  
Vol 75 (5) ◽  
pp. 1279-1290 ◽  
Author(s):  
Senjie Lin ◽  
Huan Zhang ◽  
Yubo Hou ◽  
Yunyun Zhuang ◽  
Lilibeth Miranda
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Natural Environment ◽  
Diagnostic Technique ◽  
Long Island Sound ◽  
Distance Threshold ◽  
Mirror Lake ◽  
Primer Sets ◽  
The One ◽  
High Level

ABSTRACT DNA barcoding is a diagnostic technique for species identification using a short, standardized DNA. An effective DNA barcoding marker would be very helpful for unraveling the poorly understood species diversity of dinoflagellates in the natural environment. In this study, the potential utility for DNA barcoding of mitochondrial cytochrome c oxidase 1 (cox1) and cytochrome b (cob) was assessed. Among several primer sets examined, the one amplifying a 385-bp cob fragment was most effective for dinoflagellates. This short cob fragment is easy to sequence and yet possess reasonable taxon resolution. While the lack of a uniform gap between interspecific and intraspecific distances poses difficulties in establishing a phylum-wide species-discriminating distance threshold, the variability of cob allows recognition of species within particular lineages. The potential of this cob fragment as a dinoflagellate species marker was further tested by applying it to an analysis of the dinoflagellate assemblages in Long Island Sound (LIS) and Mirror Lake in Connecticut. In LIS, a highly diverse assemblage of dinoflagellates was detected. Some taxa can be identified to the species and some to the genus level, including a taxon distinctly related to the bipolar species Polarella glacialis, and the large number of others cannot be clearly identified, due to the inadequate database. In Mirror Lake, a Ceratium species and an unresolved taxon were detected, exhibiting a temporal transition from one to the other. We demonstrate that this 385-bp cob fragment is promising for lineage-wise dinoflagellate species identification, given an adequate database.

自然环境约束下的农业发展路径探索――以冀东侯家营村为例

Rural China ◽  
2016 ◽  
Vol 13 (1) ◽  
pp. 77-102
Keyword(s):  
Natural Environment ◽  
Sandy Loam ◽  
Underground Water ◽  
Smooth Transition ◽  
Rapid Urbanization ◽  
Agrarian Economy ◽  
Loam Soil ◽  
High Level ◽  
Reform And Opening Up ◽  
Staple Crop

The natural environment—climate, topography, water and the like—influences and shapes rural development and peasant life. At the same time, social and economic development acts on and changes the local natural environment. The development of an agrarian economy is the result of the interactions among environmental, social, and economic factors—it cannot be explained as simply a causal relationship. This article analyzes the village of Houjiaying in eastern Hebei province to show how interactions among the natural environment, social factors, political forces, and economic conditions shaped the village’s development path. Before 1949, in consideration of Houjiaying’s natural constraints—sandy loam soil, a high level of underground water, and a poor irrigation infrastructure—sorghum, which requires little irrigation and is drought tolerant, was planted as the staple crop. During the collectivization period (1950s–1970s), improvement in water conservancy and drainage alleviated some of the constraints imposed by the natural environment. However, additional labor input was needed to achieve a smooth transition to growing different crops. Since China’s reform and opening up (1978), the planting of water-intensive crops and rapid urbanization have led to sinking water tables in North China. Houjiaying has been especially affected since its soil is sandy loam. This, combined with the rising costs of labor resulting from competition with work outside agriculture, has not only restricted Houjiaying’s transformation from “old farming” to “new agriculture,” but has also led to the rapid, although not complete, decline of crop farming and the “forced” development of a risky livestock industry.气候、地形、水利等自然环境影响和支配着农村发展和农民生活，同时，社会经济的发展也作用于、改变着当地的自然环境条件。农业经济是在自然、社会、经济等多种因素的互动下不断发展的，而非简单的因果关系可以解释。本文以侯家营为例，纳入其自然环境约束，探讨自然、社会、政治、经济等各种因素的互动如何影响该村的农业发展路径。解放前，沙壤土质、地下水位高、缺乏灌溉等自然环境约束导致侯家营村民依赖耐涝、低灌溉需要的高粱种植来满足生存需要;集体化时期，农田水利建设一定程度上缓解了自然环境条件的制约，但也必须投入更多劳动力才能实现作物的顺利改种;改革开放以后，高灌溉需求的作物种植和城镇化发展导致华北地下水位下降，侯家营因沙壤土质受影响明显，加之非农就业引致劳动力成本上升，不仅制约了其从“旧农业”到“新农业”的转型，而且在种植业内部也发生了迅速而不彻底的退出，“被迫”从事的养殖业发展曲折、投机性很强。 (This article is in English.)

DNA barcoding reveals a high level of mislabeling in Egyptian fish fillets

Food Control ◽  
2014 ◽  
Vol 46 ◽  
pp. 441-445 ◽  
Author(s):  
Asmaa Galal-Khallaf ◽  
Alba Ardura ◽  
Khaled Mohammed-Geba ◽  
Yaisel J. Borrell ◽  
Eva Garcia-Vazquez
Keyword(s):  
Dna Barcoding ◽  
Fish Fillets ◽  
High Level

scholarly journals THE BASIC PRINCIPLES OF AFFORDABLE LIVING SPACE IN GREEN CONSTRUCTION

2016 ◽  
Vol 1 (12) ◽  
pp. 35-39
Author(s):  
Дегтев ◽  
Ilya Degtev ◽  
Тарасенко ◽  
Viktoriya Tarasenko ◽  
Хуркова ◽  
...  
Keyword(s):  
Energy Source ◽  
Urban Development ◽  
Natural Environment ◽  
Human Activities ◽  
Life Support ◽  
Support Systems ◽  
Basic Principles ◽  
Life Support Systems ◽  
Living Space ◽  
High Level

As a result of human activities can be created a new natural environment with higher comfort indicators for urban development and is at the same time, the energy source for life support systems of buildings. The basic principles of affordable living space with a high level of comfort are a priority for "green" construction.

scholarly journals Assessing the use of the mitochondrial cox1 marker for use in DNA barcoding of red algae (Rhodophyta)

2006 ◽  
Vol 93 (8) ◽  
pp. 1101-1108 ◽  
Author(s):  
L. Robba ◽  
S. J. Russell ◽  
G. L. Barker ◽  
J. Brodie
Keyword(s):  
Dna Barcoding ◽  
Red Algae ◽  
Mitochondrial Cox1

scholarly journals NILAI CITRA KOTA DARI SUDUT PANDANG WISATAWAN (Studi Tentang Citra Kota Bandung Dampaknya Terhadap Kunjungan Ulang)

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Didin Syarifuddin
Keyword(s):  
Natural Environment ◽  
City Image ◽  
Tourist Attractions ◽  
Repeat Visit ◽  
Positive Impression ◽  
High Level ◽  
Local Traditions ◽  
The City ◽  
Repeat Visits ◽  
Determining Factor

ABSTRAKBandung dikenal dengan sebutan Kota Kembang, Paris Van Java, dan Kota Konferensi Asia Afrika. Sebutan tersebut menggambarkan Bandung memiliki citra kota dengan kesan positif dari wisatawannya. Namun  citra tersebut relatif sudah mulai bergeser, sehingga dukungan terhadap citra kota dalam bentuk menurunnya rasa sejuk pada lingkungan alamnya, memudarnya warga Bandung yang “someah hade ka semah”, berdampak pada menurunnya tingkat keramahannya, tingkat kemacetan yang cukup tinggi dengan sarana dan prasarana transportasi yang belum memadai berdampak terhadap tingkat kunjungan ulang dan menjadikan Bandung berbeda dari sebelumnya. Tujuan penelitian ini adalah untuk menjelaskan Citra Kota Bandung dalam dukungan cognitive, unique, dan affective image dampaknya terhadap kunjungan ulang wisatawan, menggunakan analisis jalur dengan ukuran sampel 100 orang. Hasil penelitian menunjukkan bahwa Citra Kota Bandung yang dibangun melalui tingkat variasi atraksi wisata, lingkungan sosial budaya dengan tradisi lokal yang diwujudkan dalam bentuk ramah terhadap tamunya gambaran memuliakan wisatawan, dukungan infrastruktur yang cukup memadai sebagai daya dukung kepariwisataan, aneka jenis hiburan tradisional dapat memberikan kesan positif bagi pengalaman wisatawan sehingga menjadi faktor penentu kunjungan ulang. Lingkungan alam kota Bandung masih cukup memberikan kesejukan dan atraksi budaya lokal yang bernilai Internasional memberikan kesan yang sangat baik sehingga membangkitkan kunjungan ulang. Terbangunnya kunjungan ulang sebagai dampak dari pengalaman wisatawan yang dirangkum dari pengetahuan selama melakukan kunjungan, menumbuhkan ketertarikan sehingga membangkitkan keinginan  wisatawan untuk melakukan kunjungan ulang.Kata kunci: Citra Kota, Cognitive Image, Unique Image, Affective Image, Repeat Visit.  ABSTRACTBandung is known as The Flower City, Paris Van Java, and the City of the Asian-African Conference. The title describes Bandung has a city image with a positive impression from its tourists. However, this image has begun to shift relatively, so that support for the image of the city in the form of a decreased sense of coolness in its natural environment, waning Bandung residents who "someah hade ka semah", have an impact on the decreasing level of hospitality, a high level of congestion with transportation facilities and infrastructure inadequate impact on the level of repeat visits and making Bandung is different from before. The purpose of this study is to explain the city image of Bandung in terms of cognitive, unique, and affective image of its impact on tourist visits, using path analysis with a sample size of 100 people. The results showed that City Image of Bandung, built through a variety of tourist attractions, a socio-cultural environment with local traditions embodied in a friendly form towards its guests, illustrated glorifying tourists, adequate infrastructure support as tourism support, various types of traditional entertainment can give a positive impression for tourists' experience so that it becomes a determining factor for repeat visits. The natural environment of Bandung is still sufficient to provide coolness and local cultural attractions of international value give a very good impression that evokes repeat visits. Rebuilding visits as a result of the experience of tourists summarized from the knowledge during the visit, fostering interest so as to arouse the desire of tourists to make a repeat visit.Keywords: City Image, Cognitive Image, Unique Image, Affective Image, Repeat Visit.

scholarly journals DNA barcodes for soil animal taxonomy

2009 ◽  
Vol 44 (8) ◽  
pp. 789-802 ◽  
Author(s):  
Rodolphe Rougerie ◽  
Thibaud Decaëns ◽  
Louis Deharveng ◽  
David Porco ◽  
Sam W. James ◽  
...  
Keyword(s):  
Biological Sciences ◽  
Dna Barcoding ◽  
Cryptic Diversity ◽  
Dna Barcodes ◽  
Soil Animal ◽  
Soil Animals ◽  
Taxonomic Impediment ◽  
Novel Method ◽  
High Level ◽  
Taxonomic System

The biodiversity of soil communities remains very poorly known and understood. Soil biological sciences are strongly affected by the taxonomic crisis, and most groups of animals in that biota suffer from a strong taxonomic impediment. The objective of this work was to investigate how DNA barcoding - a novel method using a microgenomic tag for species identification and discrimination - permits better evaluation of the taxonomy of soil biota. A total of 1,152 barcode sequences were analyzed for two major groups of animals, collembolans and earthworms, which presented broad taxonomic and geographic sampling. Besides strongly reflecting the taxonomic impediment for both groups, with a large number of species-level divergent lineages remaining unnamed so far, the results also highlight a high level (15%) of cryptic diversity within known species of both earthworms and collembolans. These results are supportive of recent local studies using a similar approach. Within an impeded taxonomic system for soil animals, DNA-assisted identification tools can facilitate and improve biodiversity exploration and description. DNA-barcoding campaigns are rapidly developing in soil animals and the community of soil biologists is urged to embrace these methods.

scholarly journals Spatio-Temporal Land-Use Changes and the Response in Landscape Pattern to Hemeroby in a Resource-Based City

2020 ◽  
Vol 9 (1) ◽  
pp. 20 ◽  
Author(s):  
Yu Tian ◽  
Bingxi Liu ◽  
Yuandong Hu ◽  
Qing Xu ◽  
Ming Qu ◽  
...  
Keyword(s):  
Landscape Pattern ◽  
Natural Environment ◽  
Human Disturbance ◽  
Human Beings ◽  
Resource Exploitation ◽  
Temporal Dimension ◽  
Landscape Pattern Metrics ◽  
Low Level ◽  
High Level ◽  
The Impact

Hemeroby is an integrated indicator used to measure the impact and degree of all human interventions on ecological components or ecosystems. The constant exploitation of resources is a strong interference of human beings to the natural environment. With the depletion of non-renewable resources, some cities with resource exploitation as their main industry—“resource-based cities”—are facing great development pressure. In order to quantify the impact of human disturbance on the natural environment and provide some scientific support for policy makers of the resource-based city, we used remote sensing images and landscape pattern metrics, introduced the synthetic hemeroby index model and analyzed the relationship between human disturbance and landscape pattern during 1990–2017. The results showed that: (1) The hemeroby in Daqing continued to rise during 1990–2017, and the main factor was the continuous expansion of the construction land and the reclamation of farmland. (2) In the areas with different hemeroby, there were significant differences in landscape pattern. In the areas with high-level hemeroby, the heterogeneity of landscape pattern was low, the aggregation among patches was high, and the shape of patches was regular, whereas the landscape pattern in the areas with medium-level hemeroby was just opposite. Although the heterogeneity of landscape pattern and the aggregation among patches were high in the areas with low-level hemeroby, the complexity of landscape was low and the shape of patches was regular. (3) In the temporal dimension, the increase of hemeroby contributed to the complexity of patch shape, the decrease of the aggregation among patches, and the fragmentation of landscape pattern. In the spatial dimension, the response in landscape pattern to human disturbance was relatively insensitive in the areas with low-level hemeroby, and this response was basically same in the high-level hemeroby and the whole study areas.

scholarly journals Conservation genetics of the vulnerable stone crayfish using DNA barcoding and microsatellites

2021 ◽  
Vol 4 ◽  
Author(s):  
Leona Lovrenčić ◽  
Lena Bonassin ◽  
Frederic Grandjean ◽  
Chris Austin ◽  
Ivana Maguire
Keyword(s):  
Genetic Diversity ◽  
Dna Barcoding ◽  
Large Scale ◽  
Hot Spot ◽  
Anthropogenic Pressure ◽  
Freshwater Species ◽  
Term Survival ◽  
Distribution Ranges ◽  
Stone Crayfish ◽  
High Level

The stone crayfish Austropotamobius torrentium is a native European freshwater species with significant population declines caused by anthropogenic pressure onto its habitats, climate change and spreading of non-native invasive crayfish and their pathogens. Large-scale DNA barcoding based on sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene revealed this species represents a highly divergent taxon whose evolutionary heritage is preserved in genetically divergent mtDNA phylogroups, with majority of them having restricted distribution ranges. Guarding in mind vanishing populations trends and potential loss of genetic diversity, effective conservation planning and monitoring are needed for ensuring the persistence and long-term survival of this endangered species. Since genetic data are often critical for defining populations for conservation and management purposes, along with DNA barcoding, we employed microsatellites to examine samples of more than 400 individuals from 17 populations across its entire distribution range in Croatia, known as the stone crayfish diversity hot spot. Microsatellite analyses revealed high level of genetic diversity and differentiation among studied populations that grouped according to their geographical position and mtDNA phylogroup. Almost all sampled populations represent a distinct genetic cluster, showing high level of differentiation and reflecting long periods of isolation. Results of genetic characterisation enabled selection of suitable donor populations for future restocking and reintroduction programs. Combination of DNA barcoding and microsatellites provided good insight into genetic diversity and population structure as well as enabled sound conservation programs for this threatened species in Croatia.

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