scholarly journals Water Buffaloes (Bubalus bubalis) Identified as an Important Reservoir of Shiga Toxin-Producing Escherichia coli in Brazil

2007 ◽  
Vol 73 (18) ◽  
pp. 5945-5948 ◽  
Author(s):  
Murilo G. Oliveira ◽  
José R. Feitosa Brito ◽  
Roberta R. Carvalho ◽  
Beatriz E. C. Guth ◽  
Tânia A. T. Gomes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South America ◽  
Shiga Toxin ◽  
Bubalus Bubalis ◽  
Human Diseases ◽  
Water Buffaloes ◽  
Genetic Profiles ◽  
First Time

ABSTRACT The presence of Shiga toxin-producing Escherichia coli (STEC) in water buffaloes is reported for the first time in South America. The prevalence of STEC ranged from 0 to 64% depending on the farm. STEC isolates exhibiting the genetic profiles stx 1 stx 2 ehxA iha saa and stx 2 ehxA iha saa predominated. Of the 20 distinct serotypes identified, more than 50% corresponded to serotypes associated with human diseases.

scholarly journals Shiga Toxin-Producing Serogroup O91 Escherichia coli Strains Isolated from Food and Environmental Samples

2017 ◽  
Vol 83 (18) ◽  
Author(s):  
Peter C. H. Feng ◽  
Sabine Delannoy ◽  
David W. Lacher ◽  
Joseph M. Bosilevac ◽  
Patrick Fach ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Shiga Toxin ◽  
Severe Illness ◽  
Content Type ◽  
Virulence Potential ◽  
Short Palindromic Repeat ◽  
Uremic Syndrome ◽  
Severe Infections ◽  
Genetic Profiles

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) strains of the O91:H21 serotype have caused severe infections, including hemolytic-uremic syndrome. Strains of the O91 serogroup have been isolated from food, animals, and the environment worldwide but are not well characterized. We used a microarray and other molecular assays to examine 49 serogroup O91 strains (environmental, food, and clinical strains) for their virulence potential and phylogenetic relationships. Most of the isolates were identified to be strains of the O91:H21 and O91:H14 serotypes, with a few O91:H10 strains and one O91:H9 strain being identified. None of the strains had the eae gene, which codes for the intimin adherence protein, and many did not have some of the genetic markers that are common in other STEC strains. The genetic profiles of the strains within each serotype were similar but differed greatly between strains of different serotypes. The genetic profiles of the O91:H21 strains that we tested were identical or nearly identical to those of the clinical O91:H21 strains that have caused severe diseases. Multilocus sequence typing and clustered regularly interspaced short palindromic repeat analyses showed that the O91:H21 strains clustered within the STEC 1 clonal group but the other O91 serotype strains were phylogenetically diverse. IMPORTANCE This study showed that food and environmental O91:H21 strains have similar genotypic profiles and Shiga toxin subtypes and are phylogenetically related to the O91:H21 strains that have caused hemolytic-uremic syndrome, suggesting that these strains may also have the potential to cause severe illness.

scholarly journals Cattle Can Be a Reservoir of Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H−Strains and a Source of Human Diseases

2000 ◽  
Vol 38 (9) ◽  
pp. 3470-3473 ◽  
Author(s):  
Martina Bielaszewska ◽  
Herbert Schmidt ◽  
Almut Liesegang ◽  
Rita Prager ◽  
Wolfgang Rabsch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Immunomagnetic Separation ◽  
Human Diseases ◽  
Escherichia Coli O157 ◽  
Separation Procedure ◽  
The Czech Republic ◽  
Clonal Relatedness ◽  
Uremic Syndrome ◽  
Genotypic Characteristics

Using the immunomagnetic separation procedure, we isolated sorbitol-fermenting (SF) Shiga toxin-producing Escherichia coli (STEC) O157:H− strains from two patients, one with hemolytic-uremic syndrome and the other with diarrhea, and from a dairy cow epidemiologically associated with the patients. The phenotypic and genotypic characteristics of all isolates were identical or closely related. Moreover, the bovine isolate showed a clonal relatedness to SF STEC O157:H− strains isolated from patients in Germany and the Czech Republic from 1988 to 1998. This is the first evidence that cattle can be a reservoir of SF STEC O157:H− and a source of human diseases.

scholarly journals First Characterization of CTX-M-15-Producing Escherichia coli Strains Belonging to Sequence Type (ST) 410, ST224, and ST1284 from Commercial Swine in South America

2016 ◽  
Vol 60 (4) ◽  
pp. 2505-2508 ◽  
Author(s):  
Ketrin C. Silva ◽  
Marina Moreno ◽  
Carlos Cabrera ◽  
Beny Spira ◽  
Louise Cerdeira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South America ◽  
Insertion Sequence ◽  
Sequence Type ◽  
Content Type ◽  
First Time ◽  
Genetic Context ◽  
New Reservoir

ABSTRACTWe report for the first time the isolation of CTX-M-15-producingEscherichia colistrains belonging to sequence type (ST) 410, ST224, and ST1284 in commercial swine in Brazil. TheblaCTX-M-15gene was located on F-::A9::B1 and C1::A9::B1 IncF-type plasmids, surrounded by a new genetic context comprising the IS26insertion sequence truncated with the ISEcp1element upstream ofblaCTX-M-15. These results reveal that commercial swine have become a new reservoir of CTX-M-15-producing bacteria in South America.

Azithromycin resistance in Shiga-toxin Producing Escherichia coli in France between 2004 and 2020 and detection of mef (C)- mph (G) genes.

2021 ◽  
Author(s):  
Etienne Bizot ◽  
Aurélie Cointe ◽  
Philippe Bidet ◽  
Patricia Mariani-Kurkdjian ◽  
Claire Amaris Hobson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
E Coli ◽  
First Time ◽  
Azithromycin Resistance

We described and characterized Shiga-toxin-producing Escherichia coli (STEC) strains with high levels of resistance to azithromycin isolated in France, between 2004 and 2020. Nine of 1715 (0.52%) STEC strains were resistant to azithromycin, with an increase since 2017. One isolate carried a plasmid-borne mef (C)- mph( G) genes association, described here for the first time in E. coli. Azithromycin resistance, although rare, needs consideration as this treatment may be useful in case of STEC infection.

scholarly journals Genomic Epidemiology of Shiga Toxin-Producing Escherichia coli Isolated from the Livestock-Food-Human Interface in South America

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1845
Author(s):  
Nicolás Galarce ◽  
Fernando Sánchez ◽  
Beatriz Escobar ◽  
Lisette Lapierre ◽  
Javiera Cornejo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South America ◽  
Shiga Toxin ◽  
Genomic Data ◽  
Genome Sequences ◽  
Multilateral Trade ◽  
Human Interface ◽  
Genomic Epidemiology ◽  
Food Borne ◽  
Sequence Types

Shiga toxin-producing Escherichia coli (STEC) are zoonotic pathogens responsible for causing food-borne diseases in humans. While South America has the highest incidence of human STEC infections, information about the genomic characteristics of the circulating strains is scarce. The aim of this study was to analyze genomic data of STEC strains isolated in South America from cattle, beef, and humans; predicting the antibiotic resistome, serotypes, sequence types (STs), clonal complexes (CCs) and phylogenomic backgrounds. A total of 130 whole genome sequences of STEC strains were analyzed, where 39.2% were isolated from cattle, 36.9% from beef, and 23.8% from humans. The ST11 was the most predicted (20.8%) and included O-:H7 (10.8%) and O157:H7 (10%) serotypes. The successful expansion of non-O157 clones such as ST16/CC29-O111:H8 and ST21/CC29-O26:H11 is highlighted, suggesting multilateral trade and travel. Virulome analyses showed that the predominant stx subtype was stx2a (54.6%); most strains carried ehaA (96.2%), iha (91.5%) and lpfA (77.7%) genes. We present genomic data that can be used to support the surveillance of STEC strains circulating at the livestock-food-human interface in South America, in order to control the spread of critical clones “from farm to table”.

Identification of the newly identified subtilase cytotoxin-encoding gene (subAB2-2) among clinical Shiga toxin-producing Escherichia coli isolates

2015 ◽  
Vol 61 (12) ◽  
pp. 990-994 ◽  
Author(s):  
Hoang Minh Son ◽  
Hoang Minh Duc ◽  
Ken-ichi Honjoh ◽  
Takahisa Miyamoto
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Virulence Genes ◽  
Important Virulence Factor ◽  
Encoding Gene ◽  
First Time ◽  
Encoding Genes ◽  
Subtilase Cytotoxin ◽  
Meat Sheep ◽  
Reference Strains

Subtilase cytotoxin (SubAB) is an important virulence factor of eae-negative Shiga toxin-producing Escherichia coli (STEC). Three variants of SubAB-encoding genes have been reported in the literature; however, the newly described subAB variant (subAB2-2) was found only in STEC strains from deer meat, sheep, and some wild animals. In this study, subAB variants were detected by PCR and DNA sequencing in 5 out of 12 (41.6%) eae-negative STEC strains isolated from patients. Most subAB-positive STEC strains (80%) harbored the subAB1 gene. The subAB2-2 gene was detected for the first time in the clinical STEC O128:H2 strain. Other virulence genes including stx1a, stx1c, stx2b, ehxA, and tia were also detected in this strain. The DNA sequence analyses of the subAB1 and subAB2-2 genes of the clinical STEC strains showed 99% and 100% identity to those of the reference strains 98NK2 and LM27558stx2, respectively. This is the first report on the detection of the subAB2-2 gene in a clinical STEC isolate.

Risk Factors for Shiga Toxin-Producing Escherichia coli-Associated Human Diseases

2014 ◽  
Vol 2 (5) ◽  
Author(s):  
Marta Rivas ◽  
Isabel Chinen ◽  
Elizabeth Miliwebsky ◽  
Marcelo Masana
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Shiga Toxin ◽  
Human Diseases

scholarly journals Comparison of Genotypes of Escherichia Coli Strains Carrying F18ab and F18ac Fimbriae from Pigs

2009 ◽  
Vol 21 (3) ◽  
pp. 359-364 ◽  
Author(s):  
Chitrita DebRoy ◽  
Elisabeth Roberts ◽  
William Scheuchenzuber ◽  
Subhashinie Kariyawasam ◽  
Bhushan M. Jayarao
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Protein Sequences ◽  
Gene Encoding ◽  
Shiga Toxins ◽  
Edema Disease ◽  
E Coli ◽  
First Time ◽  
Postweaning Diarrhea ◽  
Current Report

Escherichia coli carrying the F18 fimbriae colonize the small intestine of pigs and cause postweaning diarrhea and edema disease. There are 2 closely related antigenic variants of F18, F18ab, and F18ac. While F18ab-positive strains are known to be associated with edema disease, E. coli–carrying F18ac are known to cause diarrhea. One hundred ninety-eight E. coli isolates obtained from cases of diarrhea and edema disease in pigs isolated from feces or intestine were screened for the presence of the fedA gene encoding for F18 fimbriae. To distinguish between F18ab and F18ac, the fedA gene was sequenced in 69 F18-positive isolates/strains. The translated protein sequences of the fedA gene in the 2 variants differ; F18ac protein carries proline at amino acid residue 121, which is substituted or missing in F18ab. The F18ab- and F18ac-positive E. coli strains were compared for the presence of virulence attributes, serotypes of the isolates, and relatedness between the strains. Contrary to earlier reports that E. coli F18ab-positive strains mostly express Shiga toxin and F18ac-positive strains generally express enterotoxins, the current report shows conclusively for the first time that both variant types may carry genes for Shiga toxins and/or enterotoxins. Monoclonal antibodies produced against F18ab or F18ac fimbriae could not distinguish the strains carrying the 2 variants. Therefore, it was concluded that either of the 2 F18 variants, F18ab or F18ac, may be involved in causing postweaning diarrhea or edema disease in pigs.

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