scholarly journals UV Inactivation of SARS-CoV-2 across the UVC spectrum: KrCl* excimer, mercury-vapor, and LED sources

2021 ◽  
Author(s):  
Ben Ma ◽  
Patricia M. Gundy ◽  
Charles P. Gerba ◽  
Mark D. Sobsey ◽  
Karl G. Linden
Keyword(s):  
Hepatitis Virus ◽  
Uv Light ◽  
Mercury Vapor ◽  
Inactivation Rate ◽  
Limited Information ◽  
Murine Hepatitis Virus ◽  
Solution Ph ◽  
Healthcare Settings ◽  
Global Pandemic ◽  
Fundamental Information

Effective disinfection technology to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can help reduce viral transmissions during the on-going COVID-19 global pandemic and in the future. Ultraviolet (UV) devices emitting UVC irradiation (200-280 nm) have proven to be effective for virus disinfection, but limited information is available for SARS-CoV-2 due to the safety requirements of testing, which is limited to biosafety level (BSL) 3 laboratories. In this study, inactivation of SARS-CoV-2 in thin-film buffered aqueous solution (pH 7.4) was determined across UVC irradiation wavelengths (222 nm to 282 nm) from krypton chloride (KrCl*) excimers, a low-pressure mercury-vapor lamp, and two UVC light emitting diodes. Our results show that all tested UVC devices can effectively inactivate SARS-CoV-2, among which the KrCl* excimer had the best disinfection performance (i.e., highest inactivation rate). The inactivation rate constants of SARS-CoV-2 across wavelengths are similar to those for murine hepatitis virus (MHV) from our previous investigation, suggesting that MHV can serve as a reliable surrogate of SARS-CoV-2 with a lower BSL requirement (BSL-2) during UV disinfection tests. This study provides fundamental information for UVC action on SARS-CoV-2 and guidance for achieving reliable disinfection performance of UVC devices. IMPORTANCE UV light is an effective tool to help stem the spread of respiratory viruses and protect public health in commercial, transportation and healthcare settings. For effective use of UV, there is a need to determine the efficiency of different UV wavelengths in killing pathogens, specifically SARS-CoV-2, to support efforts to control the on-going COVID-19 global pandemic and future coronavirus-caused respiratory virus pandemics. We found that SARS-CoV-2 can be inactivated effectively using a broad range of UVC wavelengths, and 222nm provided the best disinfection performance. Interestingly, 222 nm irradiation has been found to be safe for human exposure up to thresholds that are beyond effective for inactivating viruses. Therefore, applying UV light from KrCl* excimers in public spaces can effectively help reduce viral aerosol or surface transmissions.

scholarly journals Remdesivir Metabolite GS-441524 Effectively Inhibits SARS-CoV-2 Infection in Mice Models

2020 ◽  
Author(s):  
Yingjun Li ◽  
Liu Cao ◽  
Ge Li ◽  
Feng Cong ◽  
Yunfeng Li ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Drug Administration ◽  
Half Life ◽  
Hepatitis Virus ◽  
Food And Drug Administration ◽  
Drug Candidate ◽  
Murine Hepatitis Virus ◽  
Global Pandemic ◽  
Plasma Distribution ◽  
Viral Titers

AbstractThe outbreak of coronavirus disease 2019 (COVID-19) rapidly spreads across worldwide and becomes a global pandemic. Remdesivir is the only COVID-19 treatment approved by U.S. Food and Drug Administration (FDA); however, its effectiveness is still under questioning as raised by the results of a large WHO Solidarity Trial. Herein, we report that the parent nucleotide of remdesivir, GS-441524, potently inhibits the replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Vero E6 and other cells. It exhibits good plasma distribution and longer half-life (t1/2=4.8h) in rat PK study. GS-441524 is highly efficacious against SARS-CoV-2 in AAV-hACE2 transduced mice and murine hepatitis virus (MHV) in mice, reducing the viral titers in CoV-attacked organs, without noticeable toxicity. Given that GS-441524 was the predominant metabolite of remdesivir in the plasma, the anti-COVID-19 effect of remdesivir may partly come from the effect of GS-441524. Our results also supported that GS-441524 as a promising and inexpensive drug candidate in the treatment of COVID-19 and future emerging CoVs diseases.

scholarly journals Comparing Wetland Ecosystems Service Provision under Different Management Approaches: Two Cases Study of Tianfu Wetland and Nansha Wetland in China

2021 ◽  
Vol 13 (16) ◽  
pp. 8710
Author(s):  
Yuchao Zhang ◽  
Steven Loiselle ◽  
Yimo Zhang ◽  
Qian Wang ◽  
Xia Sun ◽  
...  
Keyword(s):  
Water Quality ◽  
Ecosystem Services ◽  
Wetland Management ◽  
Nutrient Retention ◽  
Limited Information ◽  
Urban Centers ◽  
Wetland Ecosystems ◽  
Fundamental Information ◽  
Management Approaches ◽  
Study Sites

The largest blue-green infrastructures in industrialized, urbanized and developed regions in China are often multiuse wetlands, located just outside growing urban centers. These areas have multiple development pressures while providing environmental, economic, and social benefits to the local and regional populations. Given the limited information available about the tradeoffs in ecosystem services with respect to competing wetland uses, wetland managers and provincial decision makers face challenges in regulating the use of these important landscapes. In the present study, measurements made by citizen scientists were used to support a comparative study of water quality and wetland functions in two large multiuse wetlands, comparing areas of natural wetland vegetation, tourism-based wetland management and wetland agriculture. The study sites, the Nansha and Tianfu wetlands, are located in two of the most urbanized areas of China: the lower Yangtze River and Pearl River catchments, respectively. Our results indicated that the capacity of wetlands to mitigate water quality is closely related to the quality of the surrounding waters and hydrological conditions. Agricultural areas in both wetlands provided the lowest sediment and nutrient retention. The results show that the delivery of supporting ecosystem services is strongly influenced by the location and use of the wetland. Furthermore, we show that citizen scientist-acquired data can provide fundamental information on quantifying these ecosystem services, providing needed information to wetland park managers and provincial wetland administrators.

scholarly journals Characterization of murine hepatitis virus (JHM) RNA from rats with experimental encephalomyelitis

Virology ◽  
1984 ◽  
Vol 137 (2) ◽  
pp. 297-304 ◽  
Author(s):  
Dennis P. Jackson ◽  
Dean H. Percy ◽  
Vincent L. Morris
Keyword(s):  
Hepatitis Virus ◽  
Murine Hepatitis Virus

scholarly journals Variations in Disparate Regions of the Murine Coronavirus Spike Protein Impact the Initiation of Membrane Fusion

2001 ◽  
Vol 75 (6) ◽  
pp. 2792-2802 ◽  
Author(s):  
Dawn K. Krueger ◽  
Sean M. Kelly ◽  
Daniel N. Lewicki ◽  
Rosanna Ruffolo ◽  
Thomas M. Gallagher
Keyword(s):  
Tissue Culture ◽  
Membrane Fusion ◽  
Cultured Cells ◽  
Hepatitis Virus ◽  
Fusion Reaction ◽  
Spike Protein ◽  
Soluble Form ◽  
Fusion Activity ◽  
Murine Hepatitis Virus

ABSTRACT The prototype JHM strain of murine hepatitis virus (MHV) is an enveloped, RNA-containing coronavirus that has been selected in vivo for extreme neurovirulence. This virus encodes spike (S) glycoproteins that are extraordinarily effective mediators of intercellular membrane fusion, unique in their ability to initiate fusion even without prior interaction with the primary MHV receptor, a murine carcinoembryonic antigen-related cell adhesion molecule (CEACAM). In considering the possible role of this hyperactive membrane fusion activity in neurovirulence, we discovered that the growth of JHM in tissue culture selected for variants that had lost murine CEACAM-independent fusion activity. Among the collection of variants, mutations were identified in regions encoding both the receptor-binding (S1) and fusion-inducing (S2) subunits of the spike protein. Each mutation was separately introduced into cDNA encoding the prototype JHM spike, and the set of cDNAs was expressed using vaccinia virus vectors. The variant spikes were similar to that of JHM in their assembly into oligomers, their proteolysis into S1 and S2 cleavage products, their transport to cell surfaces, and their affinity for a soluble form of murine CEACAM. However, these tissue culture-adapted spikes were significantly stabilized as S1-S2 heteromers, and their entirely CEACAM-dependent fusion activity was delayed or reduced relative to prototype JHM spikes. The mutations that we have identified therefore point to regions of the S protein that specifically regulate the membrane fusion reaction. We suggest that cultured cells, unlike certain in vivo environments, select for S proteins with delayed, CEACAM-dependent fusion activities that may increase the likelihood of virus internalization prior to the irreversible uncoating process.

scholarly journals Virus-Neutralizing Monoclonal Antibody Expressed in Milk of Transgenic Mice Provides Full Protection against Virus-Induced Encephalitis

2001 ◽  
Vol 75 (6) ◽  
pp. 2803-2809 ◽  
Author(s):  
Andreas F. Kolb ◽  
Lecia Pewe ◽  
John Webster ◽  
Stanley Perlman ◽  
C. Bruce A. Whitelaw ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Neutralizing Antibodies ◽  
Defense Mechanism ◽  
Viral Infections ◽  
Hepatitis Virus ◽  
Recombinant Antibody ◽  
Lethal Dose ◽  
Neutralizing Antibody ◽  
Murine Hepatitis Virus

ABSTRACT Neutralizing antibodies represent a major host defense mechanism against viral infections. In mammals, passive immunity is provided by neutralizing antibodies passed to the offspring via the placenta or the milk as immunoglobulin G and secreted immunoglobulin A. With the long-term goal of producing virus-resistant livestock, we have generated mice carrying transgenes that encode the light and heavy chains of an antibody that is able to neutralize the neurotropic JHM strain of murine hepatitis virus (MHV-JHM). MHV-JHM causes acute encephalitis and acute and chronic demyelination in susceptible strains of mice and rats. Transgene expression was targeted to the lactating mammary gland by using the ovine β-lactoglobulin promoter. Milk from these transgenic mice contained up to 0.7 mg of recombinant antibody/ml. In vitro analysis of milk derived from different transgenic lines revealed a linear correlation between antibody expression and virus-neutralizing activity, indicating that the recombinant antibody is the major determinant of MHV-JHM neutralization in murine milk. Offspring of transgenic and control mice were challenged with a lethal dose of MHV-JHM. Litters suckling nontransgenic dams succumbed to fatal encephalitis, whereas litters suckling transgenic dams were fully protected against challenge, irrespective of whether they were transgenic. This demonstrates that a single neutralizing antibody expressed in the milk of transgenic mice is sufficient to completely protect suckling offspring against MHV-JHM-induced encephalitis.

scholarly journals In vitro analysis of the oligodendrocyte lineage in mice during demyelination and remyelination.

1990 ◽  
Vol 111 (3) ◽  
pp. 1183-1195 ◽  
Author(s):  
R Armstrong ◽  
V L Friedrich ◽  
K V Holmes ◽  
M Dubois-Dalcq
Keyword(s):  
Growth Factor ◽  
Glial Cells ◽  
Demyelinating Disease ◽  
Hepatitis Virus ◽  
Relative Proportion ◽  
Biological Properties ◽  
Murine Hepatitis Virus ◽  
Adult Mice ◽  
Igf I

A demyelinating disease induced in C57B1/6N mice by intracranial injection of a coronavirus (murine hepatitis virus strain A59) is followed by functional recovery and efficient CNS myelin repair. To study the biological properties of the cells involved in this repair process, glial cells were isolated and cultured from spinal cords of these young adult mice during demyelination and remyelination. Using three-color immunofluorescence combined with [3H]thymidine autoradiography, we have analyzed the antigenic phenotype and mitotic potential of individual glial cells. We identified oligodendrocytes with an antibody to galactocerebroside, astrocytes with an antibody to glial fibrillary acidic protein, and oligodendrocyte-type 2 astrocyte (O-2A) progenitor cells with the O4 antibody. Cultures from demyelinated tissue differed in several ways from those of age-matched controls: first, the total number of O-2A lineage cells was strikingly increased; second, the O-2A population consisted of a higher proportion of O4-positive astrocytes and cells of mixed oligodendrocyte-astrocyte phenotype; and third, all the cell types within the O-2A lineage showed enhanced proliferation. This proliferation was not further enhanced by adding PDGF, basic fibroblast growth factor (bFGF), or insulin-like growth factor I (IGF-I) to the defined medium. However, bFGF and IGF-I seemed to influence the fate of O-2A lineage cells in cultures of demyelinated tissue. Basic FGF decreased the percentage of cells expressing galactocerebroside. In contrast, IGF-I increased the relative proportion of oligodendrocytes. Thus, O-2A lineage cells from adult mice display greater phenotypic plasticity and enhanced mitotic potential in response to an episode of demyelination. These properties may be linked to the efficient remyelination achieved in this demyelinating disease.

Effect of eicosanoids on induction of procoagulant activity by murine hepatitis virus strain 3 in vitro

1991 ◽  
Vol 42 (6) ◽  
pp. 501-513 ◽  
Author(s):  
S.W. Chung ◽  
S.B. Sinclair ◽  
L.S. Fung ◽  
E.H. Cole ◽  
G.A. Levy
Keyword(s):  
Virus Strain ◽  
Hepatitis Virus ◽  
Procoagulant Activity ◽  
Murine Hepatitis Virus
Sign in / Sign up

Export Citation Format

Share Document