scholarly journals Variation in the Microbiota of Ixodes Ticks with Regard to Geography, Species, and Sex

2015 ◽  
Vol 81 (18) ◽  
pp. 6200-6209 ◽  
Author(s):  
Will Van Treuren ◽  
Loganathan Ponnusamy ◽  
R. Jory Brinkerhoff ◽  
Antonio Gonzalez ◽  
Christian M. Parobek ◽  
...  

ABSTRACTIxodes scapularisis the principal vector of Lyme disease on the East Coast and in the upper Midwest regions of the United States, yet the tick is also present in the Southeast, where Lyme disease is absent or rare. A closely related species,I. affinis, also carries the pathogen in the South but does not seem to transmit it to humans. In order to better understand the geographic diversity of the tick, we analyzed the microbiota of 104 adultI. scapularisand 13 adultI. affinisticks captured in 19 locations in South Carolina, North Carolina, Virginia, Connecticut, and New York. Initially, ticks from 4 sites were analyzed by 454 pyrosequencing. Subsequently, ticks from these sites plus 15 others were analyzed by sequencing with an Illumina MiSeq machine. By both analyses, the microbiomes of female ticks were significantly less diverse than those of male ticks. The dissimilarity between tick microbiomes increased with distance between sites, and the state in which a tick was collected could be inferred from its microbiota. The genusRickettsiawas prominent in all locations.Borreliawas also present in most locations and was present at especially high levels in one site in western Virginia. In contrast, members of the familyEnterobacteriaceaewere very common in North CarolinaI. scapularisticks but uncommon inI. scapularisticks from other sites and in North CarolinaI. affinisticks. These data suggest substantial variations in theIxodesmicrobiota in association with geography, species, and sex.

2011 ◽  
Vol 77 (10) ◽  
pp. 3244-3254 ◽  
Author(s):  
N. H. Ogden ◽  
G. Margos ◽  
D. M. Aanensen ◽  
M. A. Drebot ◽  
E. J. Feil ◽  
...  

ABSTRACTThe genetic diversity ofBorrelia burgdorferisensu stricto, the agent of Lyme disease in North America, has consequences for the performance of serological diagnostic tests and disease severity. To investigateB. burgdorferidiversity in Canada, where Lyme disease is emerging, bacterial DNA in 309 infected adultIxodes scapularisticks collected in surveillance was characterized by multilocus sequence typing (MLST) and analysis of outer surface protein C gene (ospC) alleles. Six ticks carriedBorrelia miyamotoi, and one tick carried the novel speciesBorrelia kurtenbachii. 142 ticks carriedB. burgdorferisequence types (STs) previously described from the United States. Fifty-eight ticks carriedB. burgdorferiof 1 of 19 novel or undescribed STs, which were single-, double-, or triple-locus variants of STs first described in the United States. Clonal complexes with founder STs from the United States were identified. SeventeenospCalleles were identified in 309B. burgdorferi-infected ticks. Positive and negative associations in the occurrence of different alleles in the same tick supported a hypothesis of multiple-niche polymorphism forB. burgdorferiin North America. Geographic analysis of STs andospCalleles were consistent with south-to-north dispersion of infected ticks from U.S. sources on migratory birds. These observations suggest that the genetic diversity ofB. burgdorferiin eastern and central Canada corresponds to that in the United States, but there was evidence for founder events skewing the diversity in emerging tick populations. Further studies are needed to investigate the significance of these observations for the performance of diagnostic tests and clinical presentation of Lyme disease in Canada.


2009 ◽  
Vol 14 (2) ◽  
pp. 13-16
Author(s):  
Christopher R. Brigham ◽  
Jenny Walker

Abstract The AMAGuides to the Evaluation of Permanent Impairment (AMA Guides) is the most widely used basis for determining impairment and is used in state workers’ compensation systems, federal systems, automobile casualty, and personal injury, as well as by the majority of state workers’ compensation jurisdictions. Two tables summarize the edition of the AMA Guides used and provide information by state. The fifth edition (2000) is the most commonly used edition: California, Delaware, Georgia, Hawaii, Kentucky, New Hampshire, Idaho, Indiana, Iowa, Kentucky, Massachusetts, Nevada, North Dakota, Ohio, Vermont, and Washington. Eleven states use the sixth edition (2007): Alaska, Arizona, Louisiana, Mississippi, Montana, New Mexico, Oklahoma, Pennsylvania, Rhode Island, Tennessee, and Wyoming. Eight states still commonly make use of the fourth edition (1993): Alabama, Arkansas, Kansas, Maine, Maryland, South Dakota, Texas, and West Virginia. Two states use the Third Edition, Revised (1990): Colorado and Oregon. Connecticut does not stipulate which edition of the AMA Guides to use. Six states use their own state specific guidelines (Florida, Illinois, Minnesota, New York, North Carolina, and Wisconsin), and six states do not specify a specific guideline (Michigan, Missouri, Nebraska, New Jersey, South Carolina, and Virginia). Statutes may or may not specify which edition of the AMA Guides to use. Some states use their own guidelines for specific problems and use the Guides for other issues.


2017 ◽  
Vol 61 (4) ◽  
Author(s):  
Michael J. Satlin ◽  
Liang Chen ◽  
Gopi Patel ◽  
Angela Gomez-Simmonds ◽  
Gregory Weston ◽  
...  

ABSTRACT Although the New York/New Jersey (NY/NJ) area is an epicenter for carbapenem-resistant Enterobacteriaceae (CRE), there are few multicenter studies of CRE from this region. We characterized patients with CRE bacteremia in 2013 at eight NY/NJ medical centers and determined the prevalence of carbapenem resistance among Enterobacteriaceae bloodstream isolates and CRE resistance mechanisms, genetic backgrounds, capsular types (cps), and antimicrobial susceptibilities. Of 121 patients with CRE bacteremia, 50% had cancer or had undergone transplantation. The prevalences of carbapenem resistance among Klebsiella pneumoniae, Enterobacter spp., and Escherichia coli bacteremias were 9.7%, 2.2%, and 0.1%, respectively. Ninety percent of CRE were K. pneumoniae and 92% produced K. pneumoniae carbapenemase (KPC-3, 48%; KPC-2, 44%). Two CRE produced NDM-1 and OXA-48 carbapenemases. Sequence type 258 (ST258) predominated among KPC-producing K. pneumoniae (KPC-Kp). The wzi154 allele, corresponding to cps-2, was present in 93% of KPC-3-Kp, whereas KPC-2-Kp had greater cps diversity. Ninety-nine percent of CRE were ceftazidime-avibactam (CAZ-AVI)-susceptible, although 42% of KPC-3-Kp had an CAZ-AVI MIC of ≥4/4 μg/ml. There was a median of 47 h from bacteremia onset until active antimicrobial therapy, 38% of patients had septic shock, and 49% died within 30 days. KPC-3-Kp bacteremia (adjusted odds ratio [aOR], 2.58; P = 0.045), cancer (aOR, 3.61, P = 0.01), and bacteremia onset in the intensive care unit (aOR, 3.79; P = 0.03) were independently associated with mortality. Active empirical therapy and combination therapy were not associated with survival. Despite a decade of experience with CRE, patients with CRE bacteremia have protracted delays in appropriate therapies and high mortality rates, highlighting the need for rapid diagnostics and evaluation of new therapeutics.


2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Kristen L. Knapp ◽  
Nancy A. Rice

Borrelia burgdorferi, the causative agent of Lyme disease, andBabesia microti, a causative agent of babesiosis, are increasingly implicated in the growing tick-borne disease burden in the northeastern United States. These pathogens are transmitted via the bite of an infected tick vector,Ixodes scapularis, which is capable of harboring and inoculating a host with multiple pathogens simultaneously. Clinical presentation of the diseases is heterogeneous and ranges from mild flu-like symptoms to near-fatal cardiac arrhythmias. While the reason for the variability is not known, the possibility exists that concomitant infection with bothB. burgdorferiandB. microtimay synergistically increase disease severity. In an effort to clarify the current state of understanding regarding coinfection withB. burgdorferiandB. microti, in this review, we discuss the geographical distribution and pathogenesis of Lyme disease and babesiosis in the United States, the immunological response of humans toB. burgdorferiorB. microtiinfection, the existing knowledge regarding coinfection disease pathology, and critical factors that have led to ambiguity in the literature regarding coinfection, in order to eliminate confusion in future experimental design and investigation.


Author(s):  

Abstract A new distribution map is provided for Xylella fastidiosa Wells et al. Gammaproteobacteria: Xanthomonadales: Xanthomonadaceae. Hosts: many. Information is given on the geographical distribution in Europe (Italy), Asia (Iran and Taiwan), North America (Canada, British Columbia, Ontario, Saskatchewan, USA, Alabama, Arizona, Arkansas, California, Delaware, Dstrict of Columbia, Florida, Georgia, Indiana, Kentucky, Louisiana, Maryland, Mississippi, Missouri, New Jersey, New Mexico, New York, North Carolina, Oklahoma, Oregon, Pennsylvania, South Carolina, Tennessee, Texas, Virginia, Washington and West Virginia), Central America and Caribbean (Costa Rica and Puerto Rico) and South America (Argentina, Brazil, Bahia, Espirito Santo, Goias, Minas Gerais, Para, Parana, Rio de Janeiro, Rio Grande do Sul, Santa Catarina, Sao Paulo, Sergipe, Paraguay and Venezuela).


PEDIATRICS ◽  
1973 ◽  
Vol 51 (6) ◽  
pp. 1095-1099
Author(s):  
Charles U. Lowe ◽  
Gilbert B. Forbes ◽  
Stanley Garn ◽  
George M. Owen ◽  
Nathan J. Smith ◽  
...  

In 1967 the 90th Congress of the United States attached an amendment to the Partnership for Health Act requiring the Secretary of the Department of Health, Education, and Welfare to undertake a survey of "the incidence and location of serious hunger and malnutrition–in the United States." In response to the legislative mandate the Ten-State Nutrition Survey was conducted during the years 1968 through 1970. The sample was selected from urban and rural families living in the following ten states: New York, Massachusetts, Michigan, California, Washington, Kentucky, West Virginia, Louisiana, Texas, and South Carolina. The families selected were those living in some of the census enumeration districts that made up the lowest economic quartiles of their respective states at the time of the 1960 census. During the eight years after the 1960 census the social and economic characteristics found in some of the individual enumeration districts had changed, so that there was a significant numer of families in the surveys with incomes well above the lowest income quartile. Thus, it was possible in analyzing results to make some comparisons on an economic basis. Thirty thousand families were identified in the selection process; 23,846 of these participated in the survey. Data regarding more than 80,000 individuals were obtained through interviews and 40,847 of these individuals were examined. The survey included the following: extensive demographic information on each of the participating families; information regarding food utilization of the family; a 24-hour dietary recall for infants up to 36 months of age, children 10 to 16 years of age, pregnant and lactating women, and individuals over 60 years of age.


Author(s):  

Abstract A new distribution map is provided for Apiosporina morbosa (Schwein.) v. Arx Fungi: Ascomycota: Dothideales Hosts: Stone fruit (Prunus spp.). Information is given on the geographical distribution in NORTH AMERICA, Canada, Alberta, British Columbia, Manitoba, New Brunswick, Newfoundland, Northwest, Territories, Nova Scotia, Ontario, Prince Edward Island, Quebec, Saskatchewan, Mexico, USA, Alabama, California, Colorado, Connecticut, Delaware, Florida, Georgia, Idaho, Illinois, Indiana, Iowa, Kentucky, Maine, Maryland, Massachusetts, Michigan, Mississippi, Missouri, Montana, New Jersey, New York, North Carolina, North Dakota, Ohio, Oklahoma, Oregon, Pennsylvania, South Carolina, South Dakota, Tennessee, Texas, Utah, Vermont, Virginia, Washington, West Virginia, Wisconsin.


Author(s):  

Abstract A new distribution map is provided for Xylosandrus crassiusculus Motschulsky. Coleoptera: Curculionidae. Hosts: polyphagous on many tree and shrub species. Information is given on the geographical distribution in North America (USA, Maryland, Michigan, Mississippi, Missouri, Nebraska, New Hampshire, New Jersey, New York, North Carolina, Ohio, Oklahoma, Oregon, Pennsylvania, South Carolina, Tennessee, Texas, Virginia, Washington, West Virginia), Central America and Caribbean (Costa Rica, Guatemala, Panama and Puerto Rico), South America (Argentina, Brazil, Amapa, Pernambuco, Rio de Janeiro, Sao Paulo, French Guiana and Uruguay) and Oceania (Australia, Queensland, Guam, New Caledonia, New Zealand, Palau, Papua New Guinea and Samoa).


Author(s):  
J. E. M. Mordue

Abstract A description is provided for Ustilago syntherismae. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Digitaria spp., including D. filiformis, D. ischaemum, D. sanguinalis. DISEASE: Loose smut of Digitaria. Infection sometimes dwarfs the host and causes it to branch more profusely than usual (36, 657); it is also reported that vegetative vigour is much prolonged in infected plants compared with uninfected (30, 432). Surface contamination by the large number of ustilospores when shed may result in some discolouration of the entire inflorescence including sheaths and leaf blades. GEOGRAPHICAL DISTRIBUTION: Africa: 'Congo', Gambia, Kenya, Sierra Leone, South Africa, Sudan, Uganda, Zambia, Zimbabwe; Asia: Azerbaijan, China, Republic of Georgia, India, Japan, Pakistan, Russia (central Asia, far east); Australasia: Australia: NSW (31, 225); Europe: Bulgaria, Czechoslovakia, Denmark (39, 284), Germany, Hungary, Italy, Poland, Portugal, Romania, Russia (European region), Ukraine (52, 354); North America: Canada (Ontario; 46, 3383), Mexico, USA (Alabama, Arkansas, California, Connecticut, Washington DC, Delaware, Florida, Georgia, IA, Illinois, Indiana, Kansas, Kentucky, Louisiana, Maine, MD, ME, Michigan, Montana, Missouri, MS, North Carolina, Nebraska, New Hampshire, New Jersey, New York, Ohio, Oklahoma, Pennsylvania, South Carolina, South Dakota, Tennessee, Texas, Virginia; 69, 2765); South America: Argentina, Brazil, Uruguay. (33, 634; 40, 209; 48, 2203; 50, 2756; 64, 4163). TRANSMISSION: No detailed studies have been reported; ustilospores are presumably disseminated by air currents and infection is thought to be systemic.


Author(s):  

Abstract A new distribution map is provided for Anthonomus signatus Say Coleoptera: Curculionidae Hosts: Strawberry (Fragaria ananassa), also Rubus spp. Information is given on the geographical distribution in NORTH AMERICA, Canada, Alberta, British Columbia, Manitoba, New Brunswick, Newfoundland, Nova Scotia, Ontario, Prince Edward Island, Quebec, Saskatchewan, USA, Alabama, Arkansas, Connecticut, Delaware, District of Columbia, Florida, Georgia, Illinois, Indiana, Iowa, Louisiana, Maine, Maryland, Massachusetts, Michigan, Minnesota, Missouri, New Hampshire, New Jersey, New York, North Carolina, North Dakota, Ohio, Pennsylvania, South Carolina, South Dakota, Tennessee, Texas, Virginia, Wisconsin.


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