scholarly journals Mono- and Dialkyl Glycerol Ether Lipids in Anaerobic Bacteria: Biosynthetic Insights from the Mesophilic Sulfate Reducer Desulfatibacillum alkenivorans PF2803T

2015 ◽  
Vol 81 (9) ◽  
pp. 3157-3168 ◽  
Author(s):  
Vincent Grossi ◽  
Damien Mollex ◽  
Arnauld Vinçon-Laugier ◽  
Florence Hakil ◽  
Muriel Pacton ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
Anaerobic Bacteria ◽  
Building Blocks ◽  
Carbon Chain ◽  
Ether Lipids ◽  
Glycerol Ether ◽  
Content Type ◽  
Sulfate Reducing ◽  
Cellular Lipids ◽  
Adverse Environmental Conditions

ABSTRACTBacterial glycerol ether lipids (alkylglycerols) have received increasing attention during the last decades, notably due to their potential role in cell resistance or adaptation to adverse environmental conditions. Major uncertainties remain, however, regarding the origin, biosynthesis, and modes of formation of these uncommon bacterial lipids. We report here the preponderance of monoalkyl- and dialkylglycerols (1-O-alkyl-, 2-O-alkyl-, and 1,2-O-dialkylglycerols) among the hydrolyzed lipids of the marine mesophilic sulfate-reducing proteobacteriumDesulfatibacillum alkenivoransPF2803Tgrown onn-alkenes (pentadec-1-ene or hexadec-1-ene) as the sole carbon and energy source. Alkylglycerols account for one-third to two-thirds of the total cellular lipids (alkylglycerols plus acylglycerols), depending on the growth substrate, with dialkylglycerols contributing to one-fifth to two-fifths of the total ether lipids. The carbon chain distribution of the lipids ofD. alkenivoransalso depends on that of the substrate, but the chain length and methyl-branching patterns of fatty acids and monoalkyl- and dialkylglycerols are systematically congruent, supporting the idea of a biosynthetic link between the three classes of compounds. Vinyl ethers (1-alken-1′-yl-glycerols, known as plasmalogens) are not detected among the lipids of strain PF2803T. Cultures grown on different (per)deuteratedn-alkene,n-alkanol, andn-fatty acid substrates further demonstrate that saturated alkylglycerols are not formed via the reduction of hypothetic alken-1′-yl intermediates. Our results support an unprecedented biosynthetic pathway to monoalkyl/monoacyl- and dialkylglycerols in anaerobic bacteria and suggest thatn-alkyl compounds present in the environment can serve as the substrates for supplying the building blocks of ether phospholipids of heterotrophic bacteria.

scholarly journals Correction for Grossi et al., Mono- and Dialkyl Glycerol Ether Lipids in Anaerobic Bacteria: Biosynthetic Insights from the Mesophilic Sulfate Reducer Desulfatibacillum alkenivorans PF2803T

2015 ◽  
Vol 81 (17) ◽  
pp. 6088-6088
Author(s):  
Vincent Grossi ◽  
Damien Mollex ◽  
Arnauld Vinçon-Laugier ◽  
Florence Hakil ◽  
Muriel Pacton ◽  
...  
Keyword(s):  
Anaerobic Bacteria ◽  
Sulfate Reducer ◽  
Ether Lipids ◽  
Glycerol Ether

Pilot Plant Study on Microaerobic Self-Granulated Sludge Process (Multi-Stage Reversing Flow Bioreactor: MRB)

1991 ◽  
Vol 23 (4-6) ◽  
pp. 973-980 ◽  
Author(s):  
M. Takahashi ◽  
S. Kyosai
Keyword(s):  
Pilot Plant ◽  
High Performance ◽  
Anaerobic Bacteria ◽  
Domestic Wastewater ◽  
Public Works ◽  
Symbiotic Interaction ◽  
Sulfate Reducing ◽  
Pellet Formation ◽  
Multi Stage ◽  
Domestic Wastewater Treatment

A Multi-stage Reversing flow Bioreactor (MRB) was developed by the Public Works Research Institute in 1986. It utilizes the symbiotic interaction between anaerobic bacteria (sulfate reducing bacteria) and microaerobic bacteria (Beggiatoa=filamentous sulfur oxidizing bacteria) for self-granulated pellet formation. A MRB Pilot plant for domestic wastewater treatment (design capacity was 225 m3/day) was constructed in 1988. After several modifications of the initial design, stable pellet formation and high performance were achieved. This paper describes the results of the pilot plant operation.

scholarly journals Lipidomics of facial sebum in the comparison between acne and non-acne adolescents with dark skin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Obumneme Emeka Okoro ◽  
Adebomi Adenle ◽  
Matteo Ludovici ◽  
Mauro Truglio ◽  
Federico Marini ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Building Blocks ◽  
Carbon Chain ◽  
Gas Chromatography Mass Spectrometry ◽  
Lower Percentage ◽  
Void Space ◽  
Straight Chain ◽  
Dark Skin ◽  
Cross Sectional ◽  
Weight Percentage

AbstractLipidomics is advantageous in the study of sebum perturbations occurring in acne. An extended evaluation of the sebum lipid profiles in acne-prone sebaceous areas is lacking in dark skin. Yet, there is a void space in understanding how the building blocks of sebum lipids, i.e. individual fatty acids (FAs), are intertwined with acne-prone skin. We aimed to determine the sebum lipidome in facial areas of adolescents with and without acne in Nigeria. A cross-sectional analytical study was conducted in 60 adolescents/young adults divided in 30 acne patients (15F, 15M) and 30 age and sex-matched controls. Sebum samples obtained from foreheads and cheeks were analysed separately by gas chromatography–mass spectrometry (GCMS) and thin layer chromatography (HPTLC). Distributions of sebum components were investigated with multivariate ANOVA-simultaneous component analysis (ASCA). Sebum incretion in acne was paralleled by significantly higher abundance of triglycerides, wax esters, and squalene together with monounsaturated FAs (MUFAs), and straight chain saturated FAs (SFAs), especially those with odd-carbon chain, i.e. C13:0, C15:0, and C17:0. Profiling weight/weight percentage of individual components revealed that, in acne, the free FAs (FFAs) array was shifted towards higher relative abundance of the SFAs C15:0, C16:0, and C17:0 and lower percentage of the anteiso-branched FFAs with 12, 14, 16, and 18 carbons. In acne patients, MUFAs and PUFAs were quantitatively increased and decreased on foreheads and cheeks, respectively. Relative abundance of fatty alcohols was decreased in acne independent on the site. The results indicated that acne associates with site-specific derangement of the pathways regulating the balance among odd straight-chain and branched-chain SFAs, MUFAs, which included sapienate (C16:1n-10), PUFAs, and squalene.

Diphytanyl and dibiphytanyl glycerol ether lipids of methanogenic archaebacteria

Science ◽  
1979 ◽  
Vol 203 (4375) ◽  
pp. 51-53 ◽  
Author(s):  
T. Tornabene ◽  
T. Langworthy
Keyword(s):  
Ether Lipids ◽  
Glycerol Ether

An experimental study on feature-based SLAM for multi-legged robots with RGB-D sensors

2017 ◽  
Vol 44 (4) ◽  
pp. 428-441 ◽  
Author(s):  
Michał R. Nowicki ◽  
Dominik Belter ◽  
Aleksander Kostusiak ◽  
Petr Cížek ◽  
Jan Faigl ◽  
...  
Keyword(s):  
Performance Metrics ◽  
State Of The Art ◽  
Building Blocks ◽  
Bundle Adjustment ◽  
The State ◽  
Legged Robots ◽  
Evaluation Methodology ◽  
Walking Robots ◽  
Content Type ◽  
Feature Based

Purpose This paper aims to evaluate four different simultaneous localization and mapping (SLAM) systems in the context of localization of multi-legged walking robots equipped with compact RGB-D sensors. This paper identifies problems related to in-motion data acquisition in a legged robot and evaluates the particular building blocks and concepts applied in contemporary SLAM systems against these problems. The SLAM systems are evaluated on two independent experimental set-ups, applying a well-established methodology and performance metrics. Design/methodology/approach Four feature-based SLAM architectures are evaluated with respect to their suitability for localization of multi-legged walking robots. The evaluation methodology is based on the computation of the absolute trajectory error (ATE) and relative pose error (RPE), which are performance metrics well-established in the robotics community. Four sequences of RGB-D frames acquired in two independent experiments using two different six-legged walking robots are used in the evaluation process. Findings The experiments revealed that the predominant problem characteristics of the legged robots as platforms for SLAM are the abrupt and unpredictable sensor motions, as well as oscillations and vibrations, which corrupt the images captured in-motion. The tested adaptive gait allowed the evaluated SLAM systems to reconstruct proper trajectories. The bundle adjustment-based SLAM systems produced best results, thanks to the use of a map, which enables to establish a large number of constraints for the estimated trajectory. Research limitations/implications The evaluation was performed using indoor mockups of terrain. Experiments in more natural and challenging environments are envisioned as part of future research. Practical implications The lack of accurate self-localization methods is considered as one of the most important limitations of walking robots. Thus, the evaluation of the state-of-the-art SLAM methods on legged platforms may be useful for all researchers working on walking robots’ autonomy and their use in various applications, such as search, security, agriculture and mining. Originality/value The main contribution lies in the integration of the state-of-the-art SLAM methods on walking robots and their thorough experimental evaluation using a well-established methodology. Moreover, a SLAM system designed especially for RGB-D sensors and real-world applications is presented in details.

The role of Akkermansia muciniphila in obesity, diabetes and atherosclerosis

2021 ◽  
Vol 70 (10) ◽  
Author(s):  
Alka Hasani ◽  
Saba Ebrahimzadeh ◽  
Fatemeh Hemmati ◽  
Aytak Khabbaz ◽  
Akbar Hasani ◽  
...  
Keyword(s):  
Type Species ◽  
Anaerobic Bacteria ◽  
Regulatory System ◽  
Content Type ◽  
Akkermansia Muciniphila ◽  
Link Type ◽  
Animal Trials ◽  
Obesity And Diabetes ◽  
Underlying Mechanisms

Alteration in the composition of the gut microbiota can lead to a number of chronic clinical diseases. Akkermansia muciniphila is an anaerobic bacteria constituting 3–5% of the gut microbial community in healthy adults. This bacterium is responsible for degenerating mucin in the gut; its scarcity leads to diverse clinical disorders. In this review, we focus on the role of A. muciniphila in diabetes, obesity and atherosclerosis, as well as the use of this bacterium as a next-generation probiotic. In regard to obesity and diabetes, human and animal trials have shown that A. muciniphila controls the essential regulatory system of glucose and energy metabolism. However, the underlying mechanisms by which A. muciniphila alleviates the complications of obesity, diabetes and atherosclerosis are unclear. At the same time, its abundance suggests improved metabolic disorders, such as metabolic endotoxemia, adiposity insulin resistance and glucose tolerance. The role of A. muciniphila is implicated in declining aortic lesions and atherosclerosis. Well-characterized virulence factors, antigens and cell wall extracts of A. muciniphila may act as effector molecules in these diseases. These molecules may provide novel mechanisms and strategies by which this bacterium could be used as a probiotic for the treatment of obesity, diabetes and atherosclerosis.

A logic model of the implementation of a regional workforce strategy in positive behavioural support

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Steve Noone ◽  
Alison Branch ◽  
Melissa Sherring
Keyword(s):  
Large Scale ◽  
Social Care ◽  
Building Blocks ◽  
Logic Model ◽  
Competency Framework ◽  
Health Staff ◽  
Content Type ◽  
Behavioural Support ◽  
Health And Social Care ◽  
Wide Range

Purpose Positive behavioural support (PBS) as a framework for delivering quality services is recognised in important policy documents (CQC, 2020; NICE, 2018), yet there is an absence in the literature on how this could be implemented on a large scale. The purpose of this paper is to describe a recent implementation of a workforce strategy to develop PBS across social care and health staff and family carers, within the footprint of a large integrated care system. Design/methodology/approach A logic model describes how an initial scoping exercise led to the production of a regional workforce strategy based on the PBS Competence Framework (2015). It shows how the creation of a regional steering group was able to coordinate important developmental stages and integrate multiple agencies into a single strategy to implement teaching and education in PBS. It describes the number of people who received teaching and education in PBS and the regional impact of the project in promoting cultural change within services. Findings This paper demonstrates a proof of concept that it is possible to translate the PBS Competency Framework (2015) into accredited courses. Initial scoping work highlighted the ineffectiveness of traditional training in PBS. Using blended learning and competency-based supervision and assessment, it was possible to create a new way to promote large-scale service developments in PBS supported by the governance of a new organisational structure. This also included family training delivered by family trainers. This builds on the ideas by Denne et al. (2020) that many of the necessary building blocks of implementation already exist within a system. Social implications A co-ordinated teaching and education strategy in PBS may help a wide range of carers to become more effective in supporting the people they care for. Originality/value This is the first attempt to describe the implementation of a framework for PBS within a defined geographical location. It describes the collaboration of health and social care planners and a local university to create a suite of courses built around the PBS coalition competency framework.

Click models inspired learning to rank

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Amir Hosein Keyhanipour ◽  
Farhad Oroumchian
Keyword(s):  
Random Forest ◽  
Learning To Rank ◽  
Search Time ◽  
Building Blocks ◽  
Weighted Averaging ◽  
Time Behavior ◽  
Random Forest Algorithm ◽  
Content Type ◽  
Perceived Relevance ◽  
Click Models

Purpose Incorporating users’ behavior patterns could help in the ranking process. Different click models (CMs) are introduced to model the sophisticated search-time behavior of users among which commonly used the triple of attractiveness, examination and satisfaction. Inspired by this fact and considering the psychological definitions of these concepts, this paper aims to propose a novel learning to rank by redefining these concepts. The attractiveness and examination factors could be calculated using a limited subset of information retrieval (IR) features by the random forest algorithm, and then they are combined with each other to predicate the satisfaction factor which is considered as the relevance level. Design/methodology/approach The attractiveness and examination factors of a given document are usually considered as its perceived relevance and the fast scan of its snippet, respectively. Here, attractiveness and examination factors are regarded as the click-count and the investigation rate, respectively. Also, the satisfaction of a document is supposed to be the same as its relevance level for a given query. This idea is supported by the strong correlation between attractiveness-satisfaction and the examination-satisfaction. Applying random forest algorithm, the attractiveness and examination factors are calculated using a very limited set of the primitive features of query-document pairs. Then, by using the ordered weighted averaging operator, these factors are aggregated to estimate the satisfaction. Findings Experimental results on MSLR-WEB10K and WCL2R data sets show the superiority of this algorithm over the state-of-the-art ranking algorithms in terms of P@n and NDCG criteria. The enhancement is more noticeable in top-ranked items which are reviewed more by the users. Originality/value This paper proposes a novel learning to rank based on the redefinition of major building blocks of the CMs which are the attractiveness, examination and satisfactory. It proposes a method to use a very limited number of selected IR features to estimate the attractiveness and examination factors and then combines these factors to predicate the satisfactory which is regarded as the relevance level of a document with respect to a given query.

scholarly journals Cupriavidus necator H16 Uses Flavocytochrome c Sulfide Dehydrogenase To Oxidize Self-Produced and Added Sulfide

2017 ◽  
Vol 83 (22) ◽  
Author(s):  
Chuanjuan Lü ◽  
Yongzhen Xia ◽  
Daixi Liu ◽  
Rui Zhao ◽  
Rui Gao ◽  
...  
Keyword(s):  
Gas Phase ◽  
Heterotrophic Bacteria ◽  
Sulfide Oxidation ◽  
Cupriavidus Necator ◽  
Content Type ◽  
Sulfide:Quinone Oxidoreductase ◽  
Transport Chain ◽  
Genes Encoding ◽  
Cupriavidus Necator H16 ◽  
Chemolithotrophic Bacteria

ABSTRACT Production of sulfide (H2S, HS−, and S2−) by heterotrophic bacteria during aerobic growth is a common phenomenon. Some bacteria with sulfide:quinone oxidoreductase (SQR) and persulfide dioxygenase (PDO) can oxidize self-produced sulfide to sulfite and thiosulfate, but other bacteria without these enzymes release sulfide into the medium, from which H2S can volatilize into the gas phase. Here, we report that Cupriavidus necator H16, with the fccA and fccB genes encoding flavocytochrome c sulfide dehydrogenases (FCSDs), also oxidized self-produced H2S. A mutant in which fccA and fccB were deleted accumulated and released H2S. When fccA and fccB were expressed in Pseudomonas aeruginosa strain Pa3K with deletions of its sqr and pdo genes, the recombinant rapidly oxidized sulfide to sulfane sulfur. When PDO was also cloned into the recombinant, the recombinant with both FCSD and PDO oxidized sulfide to sulfite and thiosulfate. Thus, the proposed pathway is similar to the pathway catalyzed by SQR and PDO, in which FCSD oxidizes sulfide to polysulfide, polysulfide spontaneously reacts with reduced glutathione (GSH) to produce glutathione persulfide (GSSH), and PDO oxidizes GSSH to sulfite, which chemically reacts with polysulfide to produce thiosulfate. About 20.6% of sequenced bacterial genomes contain SQR, and only 3.9% contain FCSD. This is not a surprise, since SQR is more efficient in conserving energy because it passes electrons from sulfide oxidation into the electron transport chain at the quinone level, while FCSD passes electrons to cytochrome c. The transport of electrons from the latter to O2 conserves less energy. FCSDs are grouped into three subgroups, well conserved at the taxonomic level. Thus, our data show the diversity in sulfide oxidation by heterotrophic bacteria. IMPORTANCE Heterotrophic bacteria with SQR and PDO can oxidize self-produced sulfide and do not release H2S into the gas phase. C. necator H16 has FCSD but not SQR, and it does not release H2S. We confirmed that the bacterium used FCSD for the oxidation of self-produced sulfide. The bacterium also oxidized added sulfide. The common presence of SQRs, FCSDs, and PDOs in heterotrophic bacteria suggests the significant role of heterotrophic bacteria in sulfide oxidation, participating in sulfur biogeochemical cycling. Further, FCSDs have been identified in anaerobic photosynthetic bacteria and chemolithotrophic bacteria, but their physiological roles are unknown. We showed that heterotrophic bacteria use FCSDs to oxidize self-produced sulfide and extraneous sulfide, and they may be used for H2S bioremediation.

scholarly journals Streptomycin-Induced Inflammation Enhances Escherichia coli Gut Colonization Through Nitrate Respiration

mBio ◽  
2013 ◽  
Vol 4 (4) ◽  
Author(s):  
Alanna M. Spees ◽  
Tamding Wangdi ◽  
Christopher A. Lopez ◽  
Dawn D. Kingsbury ◽  
Mariana N. Xavier ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Microbial Community ◽  
Intestinal Inflammation ◽  
Anaerobic Bacteria ◽  
Nitrate Respiration ◽  
Colonization Resistance ◽  
Content Type ◽  
E Coli ◽  
Gut Colonization ◽  
Facultative Anaerobic Bacteria

ABSTRACTTreatment with streptomycin enhances the growth of human commensalEscherichia coliisolates in the mouse intestine, suggesting that the resident microbial community (microbiota) can inhibit the growth of invading microbes, a phenomenon known as “colonization resistance.” However, the precise mechanisms by which streptomycin treatment lowers colonization resistance remain obscure. Here we show that streptomycin treatment rendered mice more susceptible to the development of chemically induced colitis, raising the possibility that the antibiotic might lower colonization resistance by changing mucosal immune responses rather than by preventing microbe-microbe interactions. Investigation of the underlying mechanism revealed a mild inflammatory infiltrate in the cecal mucosa of streptomycin-treated mice, which was accompanied by elevated expression ofNos2, the gene that encodes inducible nitric oxide synthase. In turn, this inflammatory response enhanced the luminal growth ofE. coliby nitrate respiration in aNos2-dependent fashion. These data identify low-level intestinal inflammation as one of the factors responsible for the loss of resistance toE. colicolonization after streptomycin treatment.IMPORTANCEOur intestine is host to a complex microbial community that confers benefits by educating the immune system and providing niche protection. Perturbation of intestinal communities by streptomycin treatment lowers “colonization resistance” through unknown mechanisms. Here we show that streptomycin increases the inflammatory tone of the intestinal mucosa, thereby making the bowel more susceptible to dextran sulfate sodium treatment and boosting theNos2-dependent growth of commensalEscherichia coliby nitrate respiration. These data point to the generation of alternative electron acceptors as a by-product of the inflammatory host response as an important factor responsible for lowering resistance to colonization by facultative anaerobic bacteria such asE. coli.

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