scholarly journals Orally Administered ThermostableN-Acyl Homoserine Lactonase from Bacillus sp. Strain AI96 Attenuates Aeromonas hydrophila Infection in Zebrafish

2012 ◽  
Vol 78 (6) ◽  
pp. 1899-1908 ◽  
Author(s):  
Yanan Cao ◽  
Suxu He ◽  
Zhigang Zhou ◽  
Meichao Zhang ◽  
Wei Mao ◽  
...  
Keyword(s):  
Oral Administration ◽  
Aeromonas Hydrophila ◽  
Homoserine Lactone ◽  
Signal Molecules ◽  
Fish Feed ◽  
New Approach ◽  
Content Type ◽  
Efficient Control ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum

ABSTRACTN-Acylated homoserine lactone (AHL) lactonases are capable of degrading signal molecules involved in bacterial quorum sensing and therefore represent a new approach to control bacterial infection. Here a gene responsible for the AHL lactonase activity ofBacillussp. strain AI96, 753 bp in length, was cloned and then expressed inEscherichia coli. The deduced amino acid sequence ofBacillussp. AI96 AiiA (AiiAAI96) is most similar to those of otherBacillussp. AHL lactonases (∼80% sequence identity) and was consequently categorized as a member of the metallo-β-lactamase superfamily. AiiAAI96maintains ∼100% of its activity at 10°C to 40°C at pH 8.0, and it is very stable at 70°C at pH 8.0 for at least 1 h; no otherBacillusAHL lactonase has been found to be stable under these conditions. AiiAAI96resists digestion by proteases and carp intestinal juice, and it has broad-spectrum substrate specificity. The supplementation of AiiAAI96into fish feed by oral administration significantly attenuatedAeromonas hydrophilainfection in zebrafish. This is the first report of the oral administration of an AHL lactonase for the efficient control ofA. hydrophila.

Can plants manipulate bacterial quorum sensing?

2001 ◽  
Vol 28 (9) ◽  
pp. 913 ◽  
Author(s):  
Wolfgang D. Bauer ◽  
Max Teplitski
Keyword(s):  
Higher Plants ◽  
Bacterial Species ◽  
Homoserine Lactone ◽  
Signal Molecules ◽  
Acyl Homoserine Lactone ◽  
Wide Range ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Broad Consequence ◽  
Unknown Signal

This paper originates from an address at the 8th International Symposium on Nitrogen Fixation with Non-Legumes, Sydney, NSW, December 2000 Higher plants have been found to secrete a variety of unknown signal-mimic compounds that can stimulate or inhibit behaviors in bacteria, which are regulated by N-acyl homoserine lactone (AHL) signal molecules. A wide range of bacterial species use AHLs or other signal molecules to regulate the expression of many of their genes in response to changes in population density. Thus, the ability of higher plants to specifically alter AHL-regulated behavior in bacteria by production of AHL signal-mimic compounds could be of broad consequence. We briefly review what is known about AHL signaling in bacteria and the synthesis of AHL signal-mimic compounds by plants, and then consider some of the important questions concerning the roles these plant signal-mimic compounds may play in natural encounters between plants and bacteria.

scholarly journals Host modification of a bacterial quorum-sensing signal induces a phenotypic switch in bacterial symbionts

2017 ◽  
Vol 114 (40) ◽  
pp. E8488-E8497 ◽  
Author(s):  
Cleo Pietschke ◽  
Christian Treitz ◽  
Sylvain Forêt ◽  
Annika Schultze ◽  
Sven Künzel ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Bacterial Communities ◽  
Homoserine Lactone ◽  
Innate Immune ◽  
Expression Data ◽  
Phenotypic Switch ◽  
Host Colonization ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Quorum Sensing Signals

Bacterial communities colonize epithelial surfaces of most animals. Several factors, including the innate immune system, mucus composition, and diet, have been identified as determinants of host-associated bacterial communities. Here we show that the early branching metazoan Hydra is able to modify bacterial quorum-sensing signals. We identified a eukaryotic mechanism that enables Hydra to specifically modify long-chain 3-oxo-homoserine lactones into their 3-hydroxy-HSL counterparts. Expression data revealed that Hydra’s main bacterial colonizer, Curvibacter sp., responds differentially to N-(3-hydroxydodecanoyl)-l-homoserine lactone (3OHC12-HSL) and N-(3-oxododecanoyl)-l-homoserine lactone (3OC12-HSL). Investigating the impacts of the different N-acyl-HSLs on host colonization elucidated that 3OHC12-HSL allows and 3OC12-HSL represses host colonization of Curvibacter sp. These results show that an animal manipulates bacterial quorum-sensing signals and that this modification leads to a phenotypic switch in the bacterial colonizers. This mechanism may enable the host to manipulate the gene expression and thereby the behavior of its bacterial colonizers.

scholarly journals The Bacterial Quorum-Sensing Signal MoleculeN-3-Oxo-Dodecanoyl-l-Homoserine Lactone Reciprocally Modulates Pro- and Anti-Inflammatory Cytokines in Activated Macrophages

2013 ◽  
Vol 191 (1) ◽  
pp. 337-344 ◽  
Author(s):  
Yifat Glucksam-Galnoy ◽  
Roy Sananes ◽  
Nava Silberstein ◽  
Pnina Krief ◽  
Vladimir V. Kravchenko ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Inflammatory Cytokines ◽  
Homoserine Lactone ◽  
Activated Macrophages ◽  
Bacterial Quorum Sensing ◽  
Anti Inflammatory ◽  
Bacterial Quorum

scholarly journals Genetic Differences in Barley Govern the Responsiveness to N-Acyl Homoserine Lactone

2019 ◽  
Vol 3 (3) ◽  
pp. 191-202 ◽  
Author(s):  
Abhishek Shrestha ◽  
Ahmed Elhady ◽  
Shimaa Adss ◽  
Gwendolin Wehner ◽  
Christoph Böttcher ◽  
...  
Keyword(s):  
Map Kinases ◽  
Plant Protection ◽  
Homoserine Lactone ◽  
Subsequent Treatment ◽  
Acyl Homoserine Lactone ◽  
Beneficial Effects ◽  
Enhanced Resistance ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Barley Genotypes

Enhanced resistance in barley (Hordeum vulgare) against pathogens, such as the powdery mildew-causing fungus Blumeria graminis f. sp. hordei, is of high importance. The beneficial effects of bacterial quorum sensing molecules on resistance and plant growth have been shown in different plant species. Here, we present the effects of the N-3-oxotetradecanoyl-l-homoserine lactone (oxo-C14-HSL) on the resistance of different barley genotypes. Genetically diverse accessions of barley were identified and exposed to the beneficial, oxo-C14-HSL-producing bacterium Ensifer meliloti or the pure N-acyl homoserine lactone (AHL) molecule. Metabolic profiling along with expression analysis of selected genes and physiological assays revealed that the capacity to react varies among different barley genotypes. We demonstrate that upon pretreatment with AHL molecule, AHL-primable barley genotype expresses enhanced resistance against B. graminis f. sp. hordei. We further show that pretreatment with AHL correlates with stronger activation of barley MAP kinases and regulation of defense-related PR1 and PR17b genes after a subsequent treatment with chitin. Noticeable was the stronger accumulation of lignin. Our results suggest that appropriate genetic background is required for AHL-induced priming. At the same time, they bear potential to use these genetic features for new breeding and plant protection approaches.

scholarly journals Priming winter wheat seeds with the bacterial quorum sensing signal N-hexanoyl-L-homoserine lactone (C6-HSL) shows potential to improve plant growth and seed yield

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0209460 ◽  
Author(s):  
Olena V. Moshynets ◽  
Lidia M. Babenko ◽  
Sergiy P. Rogalsky ◽  
Olga S. Iungin ◽  
Jessica Foster ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Plant Growth ◽  
Winter Wheat ◽  
Seed Yield ◽  
Homoserine Lactone ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Wheat Seeds

Response of Arabidopsis thaliana to N-hexanoyl-dl-homoserine-lactone, a bacterial quorum sensing molecule produced in the rhizosphere

Planta ◽  
2008 ◽  
Vol 229 (1) ◽  
pp. 73-85 ◽  
Author(s):  
Uta von Rad ◽  
Ilona Klein ◽  
Petre I. Dobrev ◽  
Jana Kottova ◽  
Eva Zazimalova ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Quorum Sensing Molecule

scholarly journals Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing

2014 ◽  
Vol 81 (4) ◽  
pp. 1477-1489 ◽  
Author(s):  
Nancy Weiland-Bräuer ◽  
Nicole Pinnow ◽  
Ruth A. Schmitz
Keyword(s):  
Quorum Sensing ◽  
Vibrio Fischeri ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Signal Molecules ◽  
Lethal Gene ◽  
Reporter Strain ◽  
Content Type ◽  
E Coli ◽  
Autoinducer 2

ABSTRACTTwo reporter strains were established to identify novel biomolecules interfering with bacterial communication (quorum sensing [QS]). The basic design of theseEscherichia coli-based systems comprises a gene encoding a lethal protein fused to promoters induced in the presence of QS signal molecules. Consequently, theseE. colistrains are unable to grow in the presence of the respective QS signal molecules unless a nontoxic QS-interfering compound is present. The first reporter strain designed to detect autoinducer-2 (AI-2)-interfering activities (AI2-QQ.1) contained theE. coliccdBlethal gene under the control of theE. colilsrApromoter. The second reporter strain (AI1-QQ.1) contained theVibrio fischeriluxIpromoter fused to theccdBgene to detect interference with acyl-homoserine lactones. Bacteria isolated from the surfaces of several marine eukarya were screened for quorum-quenching (QQ) activities using the established reporter systems AI1-QQ.1 and AI2-QQ.1. Out of 34 isolates, two interfered with acylated homoserine lactone (AHL) signaling, five interfered with AI-2 QS signaling, and 10 were demonstrated to interfere with both signal molecules. Open reading frames (ORFs) conferring QQ activity were identified for three selected isolates (Photobacteriumsp.,Pseudoalteromonassp., andVibrio parahaemolyticus). Evaluation of the respective heterologously expressed and purified QQ proteins confirmed their ability to interfere with the AHL and AI-2 signaling processes.

scholarly journals N-3-oxo-hexanoyl-homoserine lactone, a bacterial quorum sensing signal, enhances salt tolerance in Arabidopsis and wheat

2020 ◽  
Vol 61 (1) ◽  
Author(s):  
Qian Zhao ◽  
Xiang-Yun Yang ◽  
Yao Li ◽  
Fang Liu ◽  
Xiang-Yu Cao ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum
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