Soil Bacterial Community Shift Correlated with Change from Forest to Pasture Vegetation in a Tropical Soil

ABSTRACT The change in vegetative cover of a Hawaiian soil from forest to pasture led to significant changes in the composition of the soil bacterial community. DNAs were extracted from both soil habitats and compared for the abundance of guanine-plus-cytosine (G+C) content, by analysis of abundance of phylotypes of small-subunit ribosomal DNA (SSU rDNA) amplified from fractions with 63 and 35% G+C contents, and by phylogenetic analysis of the dominant rDNA clones in the 63% G+C content fraction. All three methods showed differences between the forest and pasture habitats, providing evidence that vegetation had a strong influence on microbial community composition at three levels of taxon resolution. The forest soil DNA had a peak in G+C content of 61%, while the DNA of the pasture soil had a peak in G+C content of 67%. None of the dominant phylotypes found in the forest soil were detected in the pasture soil. For the 63% G+C fraction SSU rDNA sequence analysis of the three most dominant members revealed that their phyla changed from Fibrobacter andSyntrophomonas assemblages in the forest soil toBurkholderia and Rhizobium–Agrobacteriumassemblages in the pasture soil.

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Effects of Simulated Nitrogen Deposition on the Bacterial Community of Urban Green Spaces

Applied Sciences ◽

10.3390/app11030918 ◽

2021 ◽

Vol 11 (3) ◽

pp. 918

Author(s):

Lingzi Mo ◽

Augusto Zanella ◽

Xiaohua Chen ◽

Bin Peng ◽

Jiahui Lin ◽

...

Keyword(s):

Bacterial Community ◽

Bacterial Diversity ◽

Negative Impact ◽

Bacterial Community Composition ◽

N Deposition ◽

Soil Bacterial Community ◽

Rrna Gene ◽

Soil Dna ◽

Urban Green ◽

Soil Bacterial

Continuing nitrogen (N) deposition has a wide-ranging impact on terrestrial ecosystems. To test the hypothesis that, under N deposition, bacterial communities could suffer a negative impact, and in a relatively short timeframe, an experiment was carried out for a year in an urban area featuring a cover of Bermuda grass (Cynodon dactylon) and simulating environmental N deposition. NH4NO3 was added as external N source, with four dosages (N0 = 0 kg N ha−2 y−1, N1 = 50 kg N ha−2 y−1, N2 = 100 kg N ha−2 y−1, N3 = 150 kg N ha−2 y−1). We analyzed the bacterial community composition after soil DNA extraction through the pyrosequencing of the 16S rRNA gene amplicons. N deposition resulted in soil bacterial community changes at a clear dosage-dependent rate. Soil bacterial diversity and evenness showed a clear trend of time-dependent decline under repeated N application. Ammonium nitrogen enrichment, either directly or in relation to pH decrease, resulted in the main environmental factor related to the shift of taxa proportions within the urban green space soil bacterial community and qualified as a putative important driver of bacterial diversity abatement. Such an impact on soil life induced by N deposition may pose a serious threat to urban soil ecosystem stability and surrounding areas.

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Taxonomic and nitrogen-cycling microbial community functional profiles of sugarcane and adjacent forest soils in Southeast Brazil

MOJ Ecology & Environmental Sciences ◽

10.15406/mojes.2021.06.00224 ◽

2021 ◽

Vol 6 (4) ◽

pp. 119-125

Author(s):

Acacio Aparecido Navarrete ◽

Eliamar Aparecida Nascimbém Pedrinho ◽

Luciano Takeshi Kishi ◽

Camila Cesário Fernandes ◽

Victoria Romancini Toledo ◽

...

Keyword(s):

Microbial Community ◽

Bacterial Community ◽

Nitrogen Cycling ◽

Forest Soil ◽

Soil Microbial Communities ◽

Soil Bacterial Community ◽

Sugarcane Production ◽

Functional Potential ◽

Southeast Brazil ◽

Soil Bacterial

Nowadays, due to the expansion of agricultural borders, it is highly desirable to increase the sustained productivity of sugarcane cultivars using the knowledge of soil microbial communities. In this study, twelve shotgun metagenomic datasets based on genomic DNA from soil were analyzed using the Metagenomics Rapid Annotation using Subsystem Technology (MG-RAST) and Statistical Analysis of Metagenomic Profiles (STAMP) to assess differential responses for the total soil bacterial community composition and nitrogen-cycling microbial community functional potential in soils from sugarcane field with pre-harvest burning and adjacent forest in dry and wet seasons in Southeast Brazil. The soil bacterial community revealed higher abundance for Actinobacteria in forest soil than sugarcane soil in dry and wet seasons, and an opposite pattern for Proteobacteria and Planctomycetes in these soils in both seasons. The results obtained in this study based on the KEEG map suggest that the forest soil has a higher nitrogen-cycling microbial community functional potential compared to the sugarcane soil, independently of the season. The gene sequences associated with carbohydrate metabolism were the most frequent in all soil metagenomes. Taken together, the results confirm previous findings regarding the effects of forest conversion to sugarcane production area, providing new insights regarding to this conversion through the prism of the seasonality and pre-harvesting method on microbially mediated nitrogen cycle in sugarcane production fields.

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The impact of land-use change on the soil bacterial community in the Loess Plateau, China

Journal of Arid Environments ◽

10.1016/j.jaridenv.2021.104469 ◽

2021 ◽

Vol 188 ◽

pp. 104469

Author(s):

Lei Zhang ◽

Junping Lv

Keyword(s):

Land Use ◽

Bacterial Community ◽

Land Use Change ◽

Loess Plateau ◽

Soil Bacterial Community ◽

The Loess Plateau ◽

Soil Bacterial ◽

The Impact

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Influences of modified biochar on metal bioavailability, metal uptake by wheat seedlings (Triticum aestivum L.) and the soil bacterial community

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2021.112370 ◽

2021 ◽

Vol 220 ◽

pp. 112370

Author(s):

Yangyang Wang ◽

Qiang Ren ◽

Tao Li ◽

Wenhao Zhan ◽

Kaixuan Zheng ◽

...

Keyword(s):

Triticum Aestivum ◽

Bacterial Community ◽

Metal Uptake ◽

Triticum Aestivum L ◽

Soil Bacterial Community ◽

Metal Bioavailability ◽

Wheat Seedlings ◽

Modified Biochar ◽

Soil Bacterial

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Effects of novel bioorganic fertilizer application on soil enzymes and bacterial community in multi-site rice paddies in China

AMB Express ◽

10.1186/s13568-021-01241-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Zuren Li ◽

Jincai Han ◽

Haodong Bai ◽

Di Peng ◽

Lifeng Wang ◽

...

Keyword(s):

Bacterial Community ◽

Field Experiments ◽

Chemical Properties ◽

Soil Bacterial Community ◽

General Function ◽

Rice Paddies ◽

Phylogenetic Groups ◽

Total N ◽

Bioorganic Fertilizer ◽

Soil Bacterial

AbstractApplication of a novel bioorganic fertilizer (BIO) has been effectively used to inhibit weeds in rice paddies. To identify changes in soil bacterial community and enzymes in response to BIO treatments, field experiments were carried out in five major rice-growing areas in China. The dominant phylogenetic groups recorded included Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Acidobacteria. Anaeromyxobacter, Bacteroides, Bifidobacterium, Escherichia- Shigella, Geobacter and Haliangium were significantly different between BIO-treatment and untreated control and aided in general function (R), amino acid transport, metabolism (E) and transcription (K) clusters. The soil chemical properties and enzyme activities were less affected by BIO at these study sites. RDA analysis showed that soil bacterial community had a significant positive correlations among northern latitude, eastern longitude, exchangeable K, total K, total P, soil pH, and total N, except for organic matter, hydrolytic N and extractable P. Overall, our work showed that application of BIO does not alter the main community structure and functional diversity of soil bacteria in rice paddies and should be encouraged for use as a sustainable weed management strategy.

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