scholarly journals Increase in Bacterial Community Diversity in Subsurface Aquifers Receiving Livestock Wastewater Input

2000 ◽  
Vol 66 (3) ◽  
pp. 956-965 ◽  
Author(s):  
Jang-Cheon Cho ◽  
Sang-Jong Kim
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
Community Diversity ◽  
Similarity Coefficients ◽  
Livestock Wastewater ◽  
Contaminated Aquifer ◽  
Bacterial Community Diversity ◽  
Rdna Sequences ◽  
Wastewater Samples ◽  
The Impact

ABSTRACT Despite intensive studies of microbial-community diversity, the questions of which kinds of microbial populations are associated with changes in community diversity have not yet been fully solved by molecular approaches. In this study, to investigate the impact of livestock wastewater on changes in the bacterial communities in groundwater, bacterial communities in subsurface aquifers were analyzed by characterizing their 16S rDNA sequences. The similarity coefficients of restriction fragment length polymorphism (RFLP) patterns of the cloned 16S ribosomal DNAs showed that the bacterial communities in livestock wastewater samples were more closely related to those in contaminated aquifer samples. In addition, calculations of community diversity clearly showed that bacterial communities in the livestock wastewater and the contaminated aquifer were much more diverse than those in the uncontaminated aquifer. Thus, the increase in bacterial-community diversity in the contaminated aquifer was assumed to be due to the infiltration of livestock wastewater, containing high concentrations of diverse microbial flora, into the aquifer. Phylogenetic analysis of the sequences from a subset of the RFLP patterns showed that the Cytophaga-Flexibacter-Bacteroidesand low-G+C gram-positive groups originating from livestock wastewater were responsible for the change in the bacterial community in groundwater. This was evidenced by the occurrence of rumen-related sequences not only in the livestock wastewater samples but also in the contaminated-groundwater samples. Rumen-related sequences, therefore, can be used as indicator sequences for fecal contamination of groundwater, particularly from livestock.

scholarly journals Diversity of Soil Bacterial Community Is Influenced by Spatial Location and Time but Not Potato Cultivar

2020 ◽  
Vol 4 (3) ◽  
pp. 225-238
Author(s):  
Kamrun Nahar ◽  
Jean-Baptiste Floc’h ◽  
Claudia Goyer ◽  
Bernie J. Zebarth ◽  
Sean Whitney
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
Common Scab ◽  
Potato Cultivar ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Streptomyces Spp ◽  
Β Diversity ◽  
Soil Bacterial ◽  
Spatial Locations

Potato cultivars susceptible to common scab were previously reported to harbor five to six times more abundant pathogenic Streptomyces spp. in the rhizosphere soils compared with tolerant cultivars. It is still unclear if the diversity of soil bacterial communities is related to the abundance of pathogenic Streptomyces spp. This study evaluated the effects of potato cultivar on the diversity of bacterial communities in three spatial locations (soil located close to the plant [SCP], in the rhizosphere soil [RS], and in the geocaulosphere soil [GS]) in 2013 and 2014. Common scab tolerant (Goldrush and Hindenburg) and susceptible cultivars (Green Mountain and Agria) were planted in a field infested with pathogenic Streptomyces spp. causing common scab. The β-diversity of the bacterial community was significantly different between years and on dates within each year according to a permutational multivariate analysis of variance. The β-diversity also varied significantly among spatial locations (i.e., SCP, RS, and GS), probably due to changes in soil properties, but did not change significantly among potato cultivars. The architecture of the bacterial network in RS in 2014 was more complex compared with 2013 with a 2.5-fold increase in the number of bacteria included according to a co-occurrence analysis. These results indicated that the soil bacterial community diversity changed temporally and spatially. However, bacterial community diversity and richness were not affected by potato cultivar, suggesting that there were no relationships between bacterial community diversity or richness and the abundance of pathogenic Streptomyces spp.

Effects of inoculation with organic-phosphorus-mineralizing bacteria on soybean (Glycine max) growth and indigenous bacterial community diversity

2017 ◽  
Vol 63 (5) ◽  
pp. 392-401 ◽  
Author(s):  
Wei Sun ◽  
Xun Qian ◽  
Jie Gu ◽  
Xiao-Juan Wang ◽  
Yang Li ◽  
...  
Keyword(s):  
Glycine Max ◽  
Carbon Source ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Organic Phosphorus ◽  
Community Diversity ◽  
Carbon Source Utilization ◽  
Soil Bacterial Communities ◽  
Bacterial Community Diversity ◽  
Soil Bacterial

Three different organic-phosphorus-mineralizing bacteria (OPMB) strains were inoculated to soil planted with soybean (Glycine max), and their effects on soybean growth and indigenous bacterial community diversity were investigated. Inoculation with Pseudomonas fluorescens Z4-1 and Brevibacillus agri L7-1 increased organic phosphorus degradation by 22% and 30%, respectively, compared with the control at the mature stage. Strains P. fluorescens Z4-1 and B. agri L7-1 significantly improved the soil alkaline phosphatase activity, average well color development, and the soybean root activity. Terminal restriction fragment length polymorphism analysis demonstrated that P. fluorescens Z4-1 and B. agri L7-1 could persist in the soil at relative abundances of 2.0%–6.4% throughout soybean growth. Thus, P. fluorescens Z4-1 and B. agri L7-1 could potentially be used in organic-phosphorus-mineralizing biofertilizers. OPMB inoculation altered the genetic structure of the soil bacterial communities but had no apparent influence on the carbon source utilization profiles of the soil bacterial communities. Principal components analysis showed that the changes in the carbon source utilization profiles of bacterial community depended mainly on the plant growth stages rather than inoculation with OPMB. The results help to understand the evolution of the soil bacterial community after OPMB inoculation.

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Author(s):  
Zhang tao ◽  
Wang Zhongke ◽  
Lv Xinhua ◽  
Dang Hanli ◽  
Zhuang Li
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Ferula sinkiangensis is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, Ferula sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of Ferula sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and variation trends of Ferula sinkiangensis rhizosphere bacterial community diversity and abundance on a fine spatial scale (Slope position and soil depth) and Found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in Ferula sinkiangensi s rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in Ferula sinkiangensis . In addition, Soil physicochemical factors jointly explained 24.28% of variation in Ferula sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of Ferula sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Tao Zhang ◽  
Zhongke Wang ◽  
Xinhua Lv ◽  
Hanli Dang ◽  
Li Zhuang
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Abstract Ferula sinkiangensis (F. sinkiangensis) is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, F. sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of F. sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and changed trend of F. sinkiangensis rhizosphere bacterial community diversity and abundance on slope position and soil depth and found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in F. sinkiangensis rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in F. sinkiangensis. In addition, Soil physicochemical factors jointly explained 24.28% of variation in F. sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of F. sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

scholarly journals Uterine and vaginal bacterial community diversity prior to artificial insemination between pregnant and nonpregnant postpartum cows1

2019 ◽  
Vol 97 (10) ◽  
pp. 4298-4304 ◽  
Author(s):  
Taylor B Ault ◽  
Brooke A Clemmons ◽  
Sydney T Reese ◽  
Felipe G Dantas ◽  
Gessica A Franco ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Artificial Insemination ◽  
Bacterial Communities ◽  
Bacterial Species ◽  
Beef Cows ◽  
Community Diversity ◽  
Rrna Gene ◽  
Bacterial Community Diversity ◽  
Hypervariable Regions ◽  
Time Of Breeding

Abstract The present study evaluated the bovine vaginal and uterine bacterial community diversity and its relationship to fertility. Postpartum beef cows (n = 68) were synchronized beginning on day −21 and ending with timed artificial insemination (TAI) on day 0. Pregnancy was diagnosed 30 d after TAI. Uterine and vaginal flushes were collected on day −21, −9, and −2 for bacterial DNA extraction to sequence the V1 to V3 hypervariable regions of the 16S rRNA gene. Results indicated a decrease in the number of bacterial species over time in the uterus of resulting pregnant and nonpregnant beef cows (P < 0.0001). Principal coordinate analyses (PCoA) depicted clustering of samples, indicating closely related bacterial communities, by day in the uterus and vagina (P < 0.0001). At day −2, uterine samples from nonpregnant and pregnant animals clustered separately (P < 0.0001), with nonpregnant animal samples clustering tightly together. Overall, the current study suggests the shift in the reproductive bacterial communities’ diversity and phylogenetic relationship leading up to the time of breeding may contribute to successful pregnancy establishment.

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Author(s):  
Zhang tao ◽  
Wang Zhongke ◽  
Lv Xinhua ◽  
Dang Hanli ◽  
Zhuang Li
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Ferula sinkiangensis is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, Ferula sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of Ferula sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and variation trends of Ferula sinkiangensis rhizosphere bacterial community diversity and abundance on a fine spatial scale (Slope position and soil depth) and Found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in Ferula sinkiangensi s rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in Ferula sinkiangensis . In addition, Soil physicochemical factors jointly explained 24.28% of variation in Ferula sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of Ferula sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

scholarly journals Soil Bacterial Community Shifts Are Driven by Soil Nutrient Availability along a Teak Plantation Chronosequence in Tropical Forests in China

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1329
Author(s):  
Zhi Yu ◽  
Kunnan Liang ◽  
Guihua Huang ◽  
Xianbang Wang ◽  
Mingping Lin ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
Soil Bacterial Community ◽  
Community Diversity ◽  
Soil Bacterial Communities ◽  
Bacterial Community Diversity ◽  
Teak Plantation ◽  
Soil Bacterial ◽  
Total Potassium ◽  
Significant Factors

Soil bacterial communities play crucial roles in ecosystem functions and biogeochemical cycles of fundamental elements and are sensitive to environmental changes. However, the response of soil bacterial communities to chronosequence in tropical ecosystems is still poorly understood. This study characterized the structures and co-occurrence patterns of soil bacterial communities in rhizosphere and bulk soils along a chronosequence of teak plantations and adjacent native grassland as control. Stand ages significantly shifted the structure of soil bacterial communities but had no significant impact on bacterial community diversity. Bacterial community diversity in bulk soils was significantly higher than that in rhizosphere soils. The number of nodes and edges in the bacterial co-occurrence network first increased and then decreased with the chronosequence. The number of strongly positive correlations per network was much higher than negative correlations. Available potassium, total potassium, and available phosphorus were significant factors influencing the structure of the bacterial community in bulk soils. In contrast, urease, total potassium, pH, and total phosphorus were significant factors affecting the structure of the bacterial community in the rhizosphere soils. These results indicate that available nutrients in the soil are the main drivers regulating soil bacterial community variation along a teak plantation chronosequence.

scholarly journals Effects of Graphene on Bacterial Community Diversity and Soil Environments of Haplic Cambisols in Northeast China

Forests ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 677 ◽  
Author(s):  
Jinfeng Song ◽  
Chengwei Duan ◽  
Ying Sang ◽  
Shaoping Wu ◽  
Jiaxin Ru ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Incubation Time ◽  
Metabolic Regulation ◽  
Diversity Index ◽  
Rank Order ◽  
Community Diversity ◽  
Regulation Mechanism ◽  
Bacterial Community Diversity ◽  
Abundance And Diversity ◽  
The Impact

Graphene is the thinnest and toughest two-dimensional nanomaterial yet discovered. However, it inevitably enters the biosphere, where it may pose potential risks to ecosystems. We investigated the impact of applied graphene concentrations on bacterial community diversity, physicochemical properties, and enzyme activities of Haplic Cambisols, the zonal soil of Northeastern China. Soils receiving 0, 10, 100, or 1000 mg kg−1 of graphene were incubated for 7, 15, 30, 60, or 90 days. Adding graphene significantly increased the community richness and diversity index of the bacterial community in Haplic Cambisols, as well as their abundances, but this impact varied with graphene concentration and incubation time. Compared with 0 mg kg−1 of graphene applied, soil bacteria abundance and diversity increased significantly during early stages of incubation (i.e., 7 and 15 days) under different concentrations of graphene, and was inhibited or remained unchanged by a longer incubation time, reaching a minima at 60 days but then following an upward trend. Graphene treatments influenced the bacterial community structure and metabolic function in Haplic Cambisols, and the bacterial community’s metabolic regulation mechanism varied with both incubation time and graphene concentration. The rank order of bacterial similarity in soils treated with graphene was 15 > 7 > 30 > 60 > 90 days. Throughout the incubation periods, except for a few unidentified bacteria, the relative abundances of Proteobacteria and Acidobacteria in the soil samples were the highest, with the number of Pseudomonas of Proteobacteria being particularly large. The rank order of bacterial abundance at the phylum level in Haplic Cambisols was 15 > 7 > 30 > 90 > 60 days. Graphene also influenced bacterial community diversity by affecting several key soil environmental factors, such as organic matter and hydrolytic nitrogen contents, as well as urease and catalase activities.

scholarly journals Spatiotemporal variation of bacterial communities in three cascade reservoirs in a southern city of China

2021 ◽  
Author(s):  
Guangqiang Cai ◽  
Tonghzou Liu ◽  
Jinsong Zhang ◽  
Haoran Song ◽  
Xiaoyan Lu ◽  
...  
Keyword(s):  
Drinking Water ◽  
Bacterial Community ◽  
Relative Abundance ◽  
Bacterial Communities ◽  
Water Sources ◽  
Spatiotemporal Variation ◽  
Community Diversity ◽  
Water Current ◽  
Cascade Reservoirs ◽  
Bacterial Community Diversity

Abstract Reservoirs are important water sources of urban drinking water supply. Bacteria play an important role in the removal of organics in reservoirs, but part of them can pose heath risk to consumers. Knowledge of bacterial community composition in drinking water sources can favour the drinking water security safeguards. This study investigated the composition and diversity of bacterial communities in the three cascade reservoirs by high-throughput Illumina HiSeq sequencing in a whole hydrological year monthly. The diversity and structure of bacterial community showed distinct spatiotemporal variation. Mycobacterium, Acinetobacter, Sphingomonas, Sphingobacterium and Pseudomonas were the genera resistant to chlorine. In addition, Acinetobacter, Arcobacter, Mycobacterium, Pseudomonas and Staphylococcus were the main potential pathogenic genera. The bacterial community diversity and the average relative abundance of potential pathogenic genera detected in wet season were higher than those in dry season. Besides, water temperature was found the main factor for the temporal variation. The spatial variation of bacterial community diversity and the average relative abundance of potential pathogenic genera were found related to water current and the degree of water pollution, respectively. The results of this study can conduce to the beneficial management of the drinking water treatment processes.

scholarly journals Distribution diversity of fungi and bacteria communities in the pure plantation of Larix gmelinii and Fraxinus mandshurica

2020 ◽  
Author(s):  
Qing Yu Wei ◽  
Wei Li ◽  
Yulong Zhang ◽  
Xing Wei
Keyword(s):  
Bacterial Community ◽  
Relative Abundance ◽  
Fungal Community ◽  
Tree Species ◽  
Bacterial Communities ◽  
Rhizosphere Soil ◽  
Larch Forest ◽  
Community Diversity ◽  
Fraxinus Mandshurica ◽  
Bacterial Community Diversity

Abstract BackgroundLarix gmelinii （larch）and Fraxinus mandshurica （ash）are two important tree species in northeast China and are infected by Ectomycorrhizal fungi and arbuscular mycorrhizal fungi, respectively.MethodUsing the high-throughput sequencing method, we analyzed the composition of Fungi and bacterial communities in the roots, Rhizosphere, and Non-rhizosphere soil of 21-year-old larch and ash pure plantations. Furthermore, we also analyzed the impact of soil environmental factors on the Fungi and bacterial community diversity according to soil nutrition. ResultsThere were differences in the fungal community diversity between larch and ash. Ascomycota increased gradually from the larch root to non-rhizosphere soil, whereas Streplophyta decreased sharply from the larch root to non-rhizosphere soil. However, the trend of Basidiomycota and Streplophyta under the ash forest was opposite to that of the larch. At the same time, it was found that Larix , Pyronemataceae _Unclassified, Cenococcum , and Ulmus were endemic to larch, whereas Anemone and Monographella were endemic to ash. The bacteria were similar under larch and ash forest. Proteobacteria decreased gradually from rhizosphere to non-rhizosphere soil, and the relative abundance of Acidobacteria , Actinobacteria , Chloroflexi , Rokubacteria , Gemmatimonadetes , Firmicutes, and Nitrospirae were the lowest in the roots of the two species. Pseudomonas , one of the Plant Growth-Promoting Rhizobacteria（PGPR）, had high relative abundance in the roots of the two tree species. The fungal and bacterial communities in the root, rhizosphere soil, and non-rhizosphere soil of the same tree species were different. The distribution diversity of the fungal and bacterial community of larch was non-rhizosphere soil > rhizosphere soil > root. The bacterial community diversity of the ash rhizosphere soil was the highest, whereas the fungal community diversity in the root was the highest. The Larix , the special fungus in the larch, were mainly distributed in the root and decreased sharply outside the root. The Pyronemataceae _Unclassified, Cenococcum, and Ulmus were mainly distributed in the rhizosphere soil. The special fungi of ash were mainly distributed in the rhizosphere. Burkholderiaceae -Unclassified, one of the PGPR, was mainly distributed in the roots of larch, but it was the opposite in the ash. Bacillus and Paenibacillus existed widely in the rhizosphere soil of ash. However, the abundance of Paenibacillus in larch was low, and it gradually increased from the root to the outside. The relative abundance of Streptomycetaceae _Unclassified was slightly high in the larch non-rhizosphere soil and ash rhizosphere soil. There was a correlation between PGPR and some fungi under the two tree species. Among them, Bacillus had a significant synergistic effect with Mortierella and Mucor under larch forest. There was a positive correlation between total nitrogen and bacteria in rhizosphere soil under larch forest, and its content was significantly higher than that of other treatments. There was a positive correlation between total phosphorus and fungi in ash rhizosphere soil, and the content was significantly lower than that in non-rhizosphere soil. However, the relationship between soil fungi and bacteria to soil nutrients was not significant.ConclusionTherefore, compared with the bacterial community, endomycorrhizal tree species have greater differences in the fungal community. The diversity of fungal and bacterial communities in ectomycorrhizal trees increase from rhizosphere soil to non-rhizosphere soil, while the diversity of fungal communities in endomycorrhizal trees is the highest in roots.

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