Effect of Direct Electric Current on the Cell Surface Properties of Phenol-Degrading Bacteria

ABSTRACT The change in cell surface properties in the presence of electric currents is of critical concern when the potential to manipulate bacterial movement with electric fields is evaluated. In this study, the effects of different direct electric currents on the cell surface properties involved in bacterial adhesion were investigated by using a mixed phenol-degrading bacterial culture in the exponential growth phase. The traits investigated were surface hydrophobicity (measured by adherence to n-octane), net surface electrostatic charge (determined by measurement of the zeta potential), and the cell surface shape and polymers (determined by scanning electron microscope analysis). The results showed that a lower current (less than 20 mA) induced no significant changes in the surface properties of phenol-degrading bacteria, that an electric current of 20 mA could increase the surface hydrophobicity and flatten the cell shape, and that a higher current (40 mA) could increase the surface extracellular substances and the net negative surface electrostatic charge. The results also revealed that the electric current effects on cell hydrophobicity varied with the suspending medium. We suggest that an electric current greater than 20 mA is not suitable for use in manipulation of the movement of the phenol-degrading bacteria, although such a current might favor the electrophoretic movement of the bacterial species.

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Cell-Surface Properties of Enterotoxigenic and CytotoxicSalmonella enteritidisandSalmonella typhimurium: Studies on Hemagglutination, Cell-Surface Hydrophobicity, Attachment to Human Intestinal Cells and Fibronectin-Binding

Microbiology and Immunology ◽

10.1111/j.1348-0421.1988.tb01405.x ◽

1988 ◽

Vol 32 (5) ◽

pp. 447-459 ◽

Cited By ~ 13

Author(s):

Suraj B. Baloda ◽

Ahmed Faris ◽

Karel Krovacek

Keyword(s):

Cell Surface ◽

Surface Properties ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Intestinal Cells ◽

Cell Surface Properties ◽

Fibronectin Binding ◽

Human Intestinal Cells

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Influence of oxygen limitation on the cell surface properties of bacteria from activated sludge

Water Science & Technology ◽

10.2166/wst.1998.0663 ◽

1998 ◽

Vol 37 (4-5) ◽

pp. 349-352 ◽

Cited By ~ 5

Author(s):

R. Palmgren ◽

F. Jorand ◽

P. H. Nielsen ◽

J. C. Block

Keyword(s):

Cell Surface ◽

Activated Sludge ◽

Surface Properties ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Growth Conditions ◽

Oxygen Limitation ◽

Cell Surface Properties ◽

Number Of Factors ◽

Math Test

Cell surface hydrophobicity is believed to be important to flocculation in activated sludge and biofilm systems. Optimization of these processes includes changes in the growth conditions of the bacteria. A number of factors influence cell surface hydrophobicity. The influence of oxygen on the cell surface hydrophobicity of 4 bacteria isolated from activated sludge was tested. The bacteria were grown in batch cultures with and without oxygen limitation. It was found that oxygen limitation generally caused a lowering of the cell surface hydrophobicity. The study also showed that there are many difficulties in measuring cell surface hydrophobicity since other cell surface properties, such as surface charge, influence the measurement methods. The MATH test was employed to establish how assay conditions influenced the results.

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Bifidobacterium longum and Bifidobacterium breve isolates from preterm and full term neonates: Comparison of cell surface properties

Anaerobe ◽

10.1016/j.anaerobe.2014.07.002 ◽

2014 ◽

Vol 28 ◽

pp. 212-215 ◽

Cited By ~ 8

Author(s):

Valérie Andriantsoanirina ◽

Anne-Claire Teolis ◽

Liu Xin Xin ◽

Marie Jose Butel ◽

Julio Aires

Keyword(s):

Cell Surface ◽

Surface Properties ◽

Bifidobacterium Longum ◽

Full Term ◽

Term Neonates ◽

Bifidobacterium Breve ◽

Cell Surface Properties

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The post‐translational modification of the C lostridium difficile flagellin affects motility, cell surface properties and virulence

Molecular Microbiology ◽

10.1111/mmi.12755 ◽

2014 ◽

Vol 94 (2) ◽

pp. 272-289 ◽

Cited By ~ 20

Author(s):

Alexandra Faulds‐Pain ◽

Susan M. Twine ◽

Evgeny Vinogradov ◽

Philippa C. R. Strong ◽

Anne Dell ◽

...

Keyword(s):

Cell Surface ◽

Surface Properties ◽

Post Translational Modification ◽

Cell Surface Properties

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Biosorption performance and cell surface properties of a fungal-based sorbent in azo dye removal coupled with textile wastewater

International Journal of Environmental Science and Technology ◽

10.1007/s13762-020-03011-5 ◽

2020 ◽

Author(s):

Hoda Nouri ◽

Ehsan Azin ◽

Aliyeh Kamyabi ◽

Hamid Moghimi

Keyword(s):

Cell Surface ◽

Surface Properties ◽

Azo Dye ◽

Dye Removal ◽

Textile Wastewater ◽

Cell Surface Properties

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Extracellular polymeric substances alter cell surface properties, toxicity, and accumulation of arsenic in Synechocystis PCC6803

Environmental Pollution ◽

10.1016/j.envpol.2020.114233 ◽

2020 ◽

Vol 261 ◽

pp. 114233 ◽

Cited By ~ 1

Author(s):

Sadiq Naveed ◽

Qingnan Yu ◽

Chunhua Zhang ◽

Ying Ge

Keyword(s):

Cell Surface ◽

Surface Properties ◽

Extracellular Polymeric Substances ◽

Cell Surface Properties ◽

Synechocystis Pcc6803

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Changes in Cell Surface Properties of Shiga Toxigenic Escherichia coli by Quercus infectoria G. Olivier

Journal of Food Protection ◽

10.4315/0362-028x-72.8.1699 ◽

2009 ◽

Vol 72 (8) ◽

pp. 1699-1704 ◽

Cited By ~ 7

Author(s):

SUPAYANG PIYAWAN VORAVUTHIKUNCHAI ◽

SAKOL SUWALAK

Keyword(s):

Escherichia Coli ◽

Cell Surface ◽

Surface Properties ◽

Cell Surface Hydrophobicity ◽

Ethanolic Extract ◽

Bacterial Cells ◽

Electron Microscopic ◽

Cell Surface Properties ◽

E Coli ◽

Quercus Infectoria

The effects of Quercus infectoria (family Fagaceae) nutgalls on cell surface properties of Shiga toxigenic Escherichia coli (STEC) were investigated with an assay of microbial adhesion to hydrocarbon. The surface of bacterial cells treated with Q. infectoria exhibited a higher level of cell surface hydrophobicity (CSH) toward toluene than did the surface of untreated cells. With 50% ethanolic extract, the CSH of the three strains of STEC O157:H7 treated with 4× MIC of the extract resulted in moderate or strong hydrophobicity, whereas at 2× MIC and MIC, the CSH of only one strain of E. coli O157:H7 was significantly affected. The 95% ethanolic extract had a significant effect on CSH of all three strains at both 4× MIC and 2× MIC but not at the MIC. The effect on bacterial CSH was less pronounced with the other STEC strains. At 4× MIC, the 50% ethanolic extract increased the CSH of all non-O157 STEC strains significantly. At 2× MIC and 4× MIC, the 95% ethanolic extract affected the CSH of E. coli O26:H11 significantly but did not affect E. coli O111:NM or E. coli O22. Electron microscopic examination revealed the loss of pili in the treated cells. The ability of Q. infectoria extract to modify hydrophobic domains enables this extract to partition the lipids of the bacterial cell membrane, rendering the membrane more permeable and allowing leakage of ions and other cell contents, which leads to cell death. Further studies are required to evaluate the effects of Q. infectoria extract in food systems or in vivo and provide support for the use of this extract as a food additive for control of these STEC pathogens.

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