scholarly journals Effects of Sample Collection and Storage Methods on Antipneumococcal Immunoglobulin A in Saliva

2003 ◽  
Vol 10 (3) ◽  
pp. 357-361 ◽  
Author(s):  
A. Nurkka ◽  
J. Obiero ◽  
H. Käyhty ◽  
J. A. G. Scott
Keyword(s):  
Liquid Nitrogen ◽  
Enzyme Inhibitors ◽  
Immunoglobulin A ◽  
Sample Collection ◽  
Protease Enzyme ◽  
Storage Methods ◽  
Commercial Kits ◽  
Capsular Antigens ◽  
And Storage ◽  
Collection Methods

ABSTRACT Saliva contains components of both the mucosal and systemic immune systems. Variable flow rates, immunoglobulin proteases, and variation in collection and storage methods all introduce differences in the estimated concentrations of antibodies. We evaluated the effect of four collection methods and three storage protocols on the concentrations of immunoglobulin A (IgA) antibodies to pneumococcal capsular antigens 1, 5, 6B, and 14 and to pneumococcal surface adhesin A (PsaA) in saliva. Specimens were collected from 30 healthy Kenyan adults by collecting drool, by pipette suction, and with two commercial kits, OraSure and Oracol. Aliquots from each specimen were snap-frozen with glycerol in liquid nitrogen or stored for 4 to 8 h at +4°C either with or without the addition of protease enzyme inhibitors prior to storage at −70°C. Anticapsular IgA concentrations were not significantly different with different collection methods, but snap-freezing the specimens in liquid nitrogen led to concentrations 41 to 47% higher than those of specimens stored by the other methods (P < 0.0005).

Comparing different sample collection and storage methods for field‐based skin microbiome research

2021 ◽  
Author(s):  
Melissa B. Manus ◽  
Sahana Kuthyar ◽  
Ana Gabriela Perroni‐Marañón ◽  
Alejandra Núñez‐ Mora ◽  
Katherine R. Amato
Keyword(s):  
Skin Microbiome ◽  
Sample Collection ◽  
Microbiome Research ◽  
Storage Methods ◽  
And Storage

Assessment of sample collection and storage methods for multicenter immunologic research in children

2008 ◽  
Vol 339 (1) ◽  
pp. 82-89 ◽  
Author(s):  
Loren A. Matheson ◽  
Trang T. Duong ◽  
Alan M. Rosenberg ◽  
Rae S.M. Yeung
Keyword(s):  
Sample Collection ◽  
Immunologic Research ◽  
Storage Methods ◽  
And Storage

scholarly journals Evaluation of sample preservation and storage methods for metaproteomics analysis of intestinal microbiomes

2021 ◽  
Author(s):  
Angie Mordant ◽  
Manuel Kleiner
Keyword(s):  
Gene Expression ◽  
Liquid Nitrogen ◽  
Room Temperature ◽  
The Other ◽  
Intestinal Microbiome ◽  
Protein Abundance ◽  
Fecal Microbiome ◽  
Storage Methods ◽  
And Storage ◽  
Measure Gene Expression

A critical step in studies of the intestinal microbiome using meta-omics approaches is the preservation of samples before analysis. Preservation is essential for approaches that measure gene expression, such as metaproteomics, which is used to identify and quantify proteins in microbiomes. Intestinal microbiome samples are typically stored by flash freezing and storage at -80 oC, but some experimental set-ups do not allow for immediate freezing of samples. In this study, we evaluated methods to preserve fecal microbiome samples for metaproteomics analyses when flash freezing is not possible. We collected fecal samples from C57BL/6 mice and stored them for 1 and 4 weeks using the following methods: flash-freezing in liquid nitrogen, immersion in RNAlater™, immersion in 95% ethanol, immersion in a RNAlater-like buffer, and combinations of these methods. After storage we extracted protein and prepared peptides for LC-MS/MS analysis to identify and quantify peptides and proteins. All samples produced highly similar metaproteomes, except for ethanol-preserved samples that were distinct from all other samples in terms of protein identifications and protein abundance profiles. Flash-freezing and RNAlater™ (or RNAlater-like treatments) produced metaproteomes that differed only slightly, with less than 0.7% of identified proteins differing in abundance. In contrast, ethanol preservation resulted in an average of 9.5% of the identified proteins differing in abundance between ethanol and the other treatments. Our results suggest that preservation at room temperature in RNAlater™,or an RNAlater-like solution, performs as well as freezing for the preservation of intestinal microbiome samples before metaproteomics analyses.

PREDIKSI PERINGKAT OBLIGASI SYARIAH DI INDONESIA

2014 ◽  
Vol 11 (3) ◽  
pp. 1-15
Author(s):  
Ike Arisanti ◽  
Isti Fadah ◽  
Novi Puspitasari
Keyword(s):  
Profit Sharing ◽  
Sample Collection ◽  
Purposive Sampling ◽  
Financial Factors ◽  
Financial Factor ◽  
Independent Variable ◽  
Rating Prediction ◽  
Collection Methods

This study purposes to analyze the influence of financial and non financial factors to prediction of the rating islamic bond in indonesia. The study used independent variable,that is financial factor (growth, size, profit sharing/fee, liquidity) and non financial factor ( secure and maturity) and dependent variable that is the rating of islamic bond. This study applied logistic regresion analysis with sample collection methods using purposive sampling. After selecting fixed criterias, there were 25 islamic bonds chosen with the numbers of 75 investigation from periods of 2010-2012. The result of this study showed that significantly effect the variable growth (X1) , size(X2), profit sharing/ fee (X3), liquidity (X4), secure (X5), maturity (X6) simultaneously to the rating prediction of islamic bond in indonesia. Partially, variable variables of growth (X1) , size (X2), profit sharing/ fee (X3) which referred not significant affecting to the rating prediction of islamic bond in indonesia. Meanwhile, variables of liquidity (X4), secure (X5), maturity ( X6) referred significant affecting to the rating prediction of islamic bond in indonesia.

Comparison of Procedures for Isolation of Monoterpene Hydrocarbons from Fresh Needles of Picea abies and Picea omorica

2000 ◽  
Vol 65 (7) ◽  
pp. 1073-1081 ◽  
Author(s):  
Valerie Holubová ◽  
Iva Chvílíčková ◽  
Vlastimil Kubáň
Keyword(s):  
Picea Abies ◽  
Liquid Nitrogen ◽  
Steam Distillation ◽  
Extraction Efficiency ◽  
Total Content ◽  
Storage Conditions ◽  
Fresh Weight ◽  
Hexane Extraction ◽  
Monoterpene Hydrocarbons ◽  
And Storage

Extraction procedures (steam distillation, supercritical fluid extraction and solvent extraction) for isolation of monoterpene hydrocarbons from fresh needles of Picea abies and Picea omorica were optimised. The procedures were compared with the aim of minimizing consumption of needles and improving the extraction efficiency and repeatability. An influence of homogenisation procedures and storage conditions (liquid nitrogen, -18 and 4 °C) on the total content and composition of essential oils was studied. Cryogenic grinding (liquid nitrogen) combined with the extraction with cold hexane (extraction time 2 h) and subsequent GC-MS determination in freshly homogenised needles gives the best results (1.5-4 times better extraction efficiency, RSD < 10% for P. abies and < 25% for P. omorica). Limits of detections (3 S/N) for individual monoterpene hydrocarbons from units to tens of ng/g and recoveries 97.2-101.4% were found in fresh needles (calculated to fresh weight). While cooling to 4 °C is unacceptable, freezing at -18 °C for the period of 18 days in the dark gives also good results.

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