scholarly journals Role of Macrophage Migration Inhibitory Factor in Otitis Media with Effusion in Adults

2003 ◽  
Vol 10 (3) ◽  
pp. 417-422 ◽  
Author(s):  
Shin Kariya ◽  
Mitsuhiro Okano ◽  
Katsuya Aoji ◽  
Michiya Kosaka ◽  
Emiko Chikumoto ◽  
...  
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Otitis Media With Effusion ◽  
Fluid Collection ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Tnf Α

ABSTRACT Otitis media with effusion (OME) is one of the most common ear diseases. Bacterial endotoxins and several inflammatory cytokines appear to be involved in the pathogenesis of OME in children; however, little is known of the immunological aspects of the onset of OME in adults. We sought to determine the presence of macrophage migration inhibitory factor (MIF) as well as interleukin 1β (IL-1β), tumor necrosis factor alpha (TNF-α), RANTES (regulated upon activation, normal T-cell expressed and presumably secreted), and endotoxin in middle ear effusions (MEEs) from adult patients with OME. In addition, the levels of MIF in MEEs from adults and children were compared. MEE was obtained from 95 adults and 11 children. The levels of MIF, IL-1β, TNF-α, and RANTES were determined by enzyme-linked immunosorbent assay, and the concentrations of endotoxin and total protein were determined by the Endospec assay and bicinchoninic acid assay, respectively. MIF was detected in 97.9% of the MEEs from adults, while endotoxin, IL-1β, TNF-α, and RANTES were detected in 96.8, 12.6, 5.3, and 43.9%, respectively. In addition, the level of MIF was significantly higher than those of endotoxin, IL-1β, and TNF-α. A positive correlation between the levels of MIF and endotoxin was observed. MIF and endotoxin were detected in 81.8 and 72.7%, respectively, of the MEEs from the children. The level of MIF was significantly higher in the children, and conversely that of endotoxin was significantly higher in the adults. These results suggest that the interaction between MIF and endotoxin may promote fluid collection in the middle ear, particularly in adults.

Up-regulation of macrophage migration inhibitory factor induced by endotoxin in experimental otitis media with effusion in mice

2008 ◽  
Vol 128 (7) ◽  
pp. 750-755 ◽  
Author(s):  
Shin Kariya ◽  
Patricia A. Schachern ◽  
Sebahattin Cureoglu ◽  
Vladimir Tsuprun ◽  
Mitsuhiro Okano ◽  
...  
Keyword(s):  
Otitis Media ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Otitis Media With Effusion ◽  
Inhibitory Factor ◽  
Macrophage Migration

scholarly journals Macrophage Migration Inhibitory Factor as a Novel Biomarker of Portopulmonary Hypertension

2016 ◽  
Vol 6 (4) ◽  
pp. 498-507 ◽  
Author(s):  
Hilary M. DuBrock ◽  
Josanna M. Rodriguez-Lopez ◽  
Barbara L. LeVarge ◽  
Michael P. Curry ◽  
Paul A. VanderLaan ◽  
...  
Keyword(s):  
Liver Disease ◽  
Migration Inhibitory Factor ◽  
Predictive Value ◽  
Pulmonary Circulation ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Heart Catheterization ◽  
Macrophage Migration ◽  
Portopulmonary Hypertension

Portopulmonary hypertension (POPH) is a poorly understood complication of liver disease associated with significant morbidity and mortality. We sought to identify novel biomarkers of POPH disease presence and severity. We performed a prospective, multicenter, case-control study involving patients with liver disease undergoing right heart catheterization. POPH cases were defined as a mean pulmonary arterial pressure (mPAP) ≥25 mmHg and pulmonary vascular resistance (PVR) >240 dynes·s·cm−5. Plasma samples were collected from the systemic and pulmonary circulation, and antibody microarray was used to identify biomarkers. Characterization and validation of a candidate cytokine, macrophage migration inhibitory factor (MIF), was performed using enzyme-linked immunosorbent assay. Continuous variables were compared using a Mann-Whitney U test and correlated with disease severity using Spearman correlation. MIF levels were elevated in both the systemic and pulmonary circulation in patients with POPH compared with controls (median MIF level [interquartile range] in systemic circulation: 46.68 ng/mL [32.31–76.04] vs. 31.19 ng/mL [26.92–42.17], P = 0.009; in pulmonary circulation: 49.59 ng/mL [35.90–108.80] vs. 37.78 [21.78–45.53], P = 0.002). In patients with POPH, MIF levels were positively correlated with PVR ( r = 0.58, P = 0.006) and inversely correlated with cardiac output ( r = −0.57, P = 0.007). MIF >60 ng/mL or tricuspid regurgitation gradient >50 mmHg had a 92% sensitivity and specificity for the diagnosis of POPH, with a positive predictive value of 86% and a negative predictive value of 96%. MIF is a promising novel biomarker of POPH disease presence and severity in patients with liver disease and portal hypertension.

scholarly journals Adrenomedullin Is Both Proinflammatory and Antiinflammatory: Its Effects on Gene Expression and Secretion of Cytokines and Macrophage Migration Inhibitory Factor in NR8383 Macrophage Cell Line

Endocrinology ◽  
2005 ◽  
Vol 146 (3) ◽  
pp. 1321-1327 ◽  
Author(s):  
Louisa Y. F. Wong ◽  
Bernard M. Y. Cheung ◽  
Yuk-Yin Li ◽  
Fai Tang
Keyword(s):  
Inflammatory Response ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Culture Media ◽  
Inhibitory Factor ◽  
Macrophage Migration ◽  
Macrophage Cell ◽  
Gene Expressions ◽  
Initiation And Propagation ◽  
Tnf Α

Adrenomedullin (ADM) is a potent vasorelaxant peptide that plays important roles in cardiovascular homeostasis and inflammatory response. ADM derived from macrophages is one of the major sources of ADM that is produced in the inflammatory process. To assess the functions of ADM in inflammation, we studied the temporal changes in ADM production and its effect on secretion of macrophage migration inhibitory factor (MIF) and cytokine response of NR8383 rat macrophages activated by lipopolysaccharide (LPS). NR8383 cells were stimulated by LPS in the absence and presence of exogenous ADM, and the concentrations of ADM, MIF, and proinflammatory cytokines (IL-6, TNF-α, and IL-1β) in the culture media and gene expressions of the cells were measured. We confirmed that the secretion and mRNA expression of ADM in the macrophages were markedly increased by LPS. ADM increased initial secretion of MIF and IL-1β from both nonstimulated and LPS-stimulated cells, and it also increased basal and LPS-induced IL-6 secretion of the cells by 2- to 15-fold. However, it reduced secretion of TNF-α from LPS-stimulated cells by 34–56%. Our results suggest that ADM modulates MIF secretion and cytokine production and plays important roles in both the initiation and propagation of the inflammatory response.

scholarly journals Efficacy of Sertraline Plus Placebo or Add-On Celecoxib in Major Depressive Disorder: Macrophage Migration Inhibitory Factor as a Promising Biomarker for Remission After Sertraline—Results From a Randomized Controlled Clinical Trial

2021 ◽  
Vol 12 ◽  
Author(s):  
Maria S. Simon ◽  
Bianka Burger ◽  
Elif Weidinger ◽  
Gara Arteaga-Henríquez ◽  
Peter Zill ◽  
...  
Keyword(s):  
Major Depressive Disorder ◽  
Depressive Disorder ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Depression Symptoms ◽  
Macrophage Migration ◽  
Major Depressive ◽  
Baseline Levels

Introduction: Previous research delivers strong indications that inflammatory activation leads to treatment resistance in a subgroup of patients with Major Depressive Disorder (MDD). Thus, tailored interventions are needed. The present study aimed to find potential biomarkers that may enable patients to be stratified according to immune activation.Methods: A phase IIa randomized placebo-controlled trial was performed to assess levels of inflammatory compounds in responders/remitters and non-responders/non-remitters to sertraline plus celecoxib (n = 20) and sertraline plus placebo (n = 23). Levels of macrophage migration inhibitory factor, neopterin, and tumor necrosis factor alpha were determined by enzyme-linked immunosorbent assay; response and remission were measured by reduction of the Montgomery Åsberg Depression Rating Scale score.Results: Both treatment groups showed a significant decline in depression symptoms, but no difference was found between groups. A clear pattern emerged only for macrophage migration inhibitory factor: placebo remitters showed significantly lower baseline levels than non-remitters (a similar trend was seen in responders and non-responders) while celecoxib responders showed a trend for higher baseline levels than non-responders.Conclusion: Small subsample sizes are a notable limitation, wherefore results are preliminary. However, the present study provides novel insights by suggesting macrophage migration inhibitory factor as a promising biomarker for treatment choice.The trial was registered in EU Clinical Trials Register (EU-CTR): https://www.clinicaltrialsregister.eu/ctr-search/trial/2009-011990-34/DE, EudraCT-No.: 2009-011990-34.

scholarly journals A genetic role for macrophage migration inhibitory factor (MIF) in Epinephelus awoara infected with Vibrio parahaemolyticus

2016 ◽  
Vol 14 (3) ◽  
pp. 184-189
Author(s):  
Xiaojin Xu ◽  
Benhong Sang ◽  
Gang Luo
Keyword(s):  
Migration Inhibitory Factor ◽  
Vibrio Parahaemolyticus ◽  
Macrophage Migration Inhibitory Factor ◽  
Head Kidney ◽  
Inhibitory Factor ◽  
Macrophage Migration ◽  
Mrna Levels ◽  
Tissue Levels ◽  
Tnf Α ◽  
Epinephelus Awoara

Macrophage migration inhibitory factor (MIF) is a key pro-inflammatory cytokine in immuno-inflammatory diseases. For the first time, we examined the expression of MIF in Epinephelus awoara ( E. awoara). MIF expressions have been detected in the head kidney, spleen, liver, brain, intestine, gill, heart, stomach, and muscle of E. awoara infected with Vibrio parahaemolyticus. The mRNA levels observed in infected groupers were higher than those in healthy groupers. MIF, tumor necrosis factor-α (TNF-α), and interleukin-1 (IL-1) tissue levels have been measured by ELISA. A significant increase in MIF, TNF-α, and IL-1 tissue levels have been found in the treatment groups compared with those in controls. MIF, TNF-α and IL-1 tissue levels in the spleen, head kidney, intestine, and liver of E. awoara during the challenge trial with V. parahaemolyticus were significantly higher than those in controls. There was evidence of functions of MIF in a positive feedback loop with TNF-α and IL-1 that could perpetuate the inflammatory process in grouper infected with V. parahaemolyticus. In conclusion, these results indicated that MIF was related to pathogen-induced immune response.

scholarly journals Macrophage Migration Inhibitory Factor Levels in Gingival Crevicular Fluid, Saliva, and Serum of Chronic Periodontitis Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Yveth Marlene Ortiz-García ◽  
Trinidad García-Iglesias ◽  
Gabriela Morales-Velazquez ◽  
Blanca Patricia Lazalde-Ramos ◽  
Guillermo Moisés Zúñiga-González ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Chronic Periodontitis ◽  
Macrophage Migration Inhibitory Factor ◽  
Gingival Crevicular Fluid ◽  
Clinical Signs ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Cross Sectional ◽  
Crevicular Fluid

Chronic periodontitis (CP) is an infection that affects the teeth supporting structure. Macrophage migration inhibitory factor (MIF) is an important effector cytokine of the innate immune system. Due to its functional characteristics, MIF may be involved in the immunopathology of CP. The aim of the present study was to evaluate MIF levels in gingival crevicular fluid (GCF), saliva, and serum of CP patients. A cross-sectional study was conducted on 60 subjects divided into two groups: subjects with CP (n= 30) and periodontally healthy subjects without CP (n=30). MIF was quantified in GCF, saliva, and serum of all participants by enzyme-linked immunosorbent assay. MIF concentrations were higher in GCF, saliva, and serum in the group with CP compared with the group without CP and a higher MIF concentration was observed in GCF (p=0.001) and saliva (p=0.009) in the group with CP. MIF intragroup comparisons between fluids demonstrated significant high levels of MIF in saliva compared with GCF and serum in both study groups (p<0.05). A positive correlation was found between clinical signs and MIF concentration in GCF (p<0.05). There is an association between the MIF and the clinical signs of the disease. Therefore, MIF could have an important role in the pathology and progression of CP.

scholarly journals Factors Associated With Macrophage Migration Inhibitory Factor in Neonates: a Pilot Study

2021 ◽  
Author(s):  
Ji Sook Park ◽  
Jin Su Jun ◽  
Ji-Hyun Seo ◽  
Jae Young Lim ◽  
Chan-Hoo Park ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Associated Factors ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Preterm Neonates ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Clinical Factors ◽  
Term Neonates ◽  
Factors Associated

Abstract Objective: The study was to conduct an investigation of associated factor with macrophage migration inhibitory factor (MIF) in neonates.Results: MIF was measured by Enzyme-linked Immunosorbent Assay (ELISA) using sera obtained from sick neonates on the median postnatal 2.0nd day. Clinical details were reviewed from medical record and grouped into preterm and near or full-term neonates based on 34 weeks of gestation at birth. Statistical analyses were performed between MIF concentration and clinical factors. In total, 77 neonates consisted of preterm (n = 42) and term neonates (n = 35) were included. The median value of plasma MIF was higher in preterm neonates (7,037.6 pg/mL, IQR: 3,285.2-12,267.4) than in the others (3,968.8 pg/mL, IQR: 2,566.4-6,995.2, P = 0.013). Among 42 preterm neonates, those with necrotizing enterocolitis (NEC) in later had higher MIF concentration (12,170.9 pg/mL, IQR: 8,353.3-23,537.7, n = 9) compared with the others without NEC (5,189.6 pg/mL, IQR: 3,220.2-9,097.9, n = 33, P = 0.016). Associated factors of higher MIF in neonates were preterm neonates and NEC.

Role of Macrophage Migration Inhibitory Factor in Paranasal Sinus Mucocele

2005 ◽  
Vol 19 (6) ◽  
pp. 554-559 ◽  
Author(s):  
Shin Kariya ◽  
Mitsuhiro Okano ◽  
Katsuya Aoji ◽  
Tomoko Nakashima ◽  
Norio Kasai ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Paranasal Sinus ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Tumor Necrosis ◽  
Inhibitory Factor ◽  
Interleukin 1Β ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Necrosis Factor

Background Little is known about the immunologic aspects and the pathogenesis of the paranasal sinus mucocele. Methods The fluids of paranasal sinus mucoceles were obtained from 12 subjects. The concentration of macrophage migration inhibitory factor (MIF), interleukin 1β, tumor necrosis factor a, and regulated on activation normal T cell expressed and secreted (RANTES) were determined by enzyme-linked immunosorbent assay, and the levels of endotoxin were detected with kinetic Turbidimetric Assay. Results MIF and endotoxin were detected in the fluid of all samples, whereas interleukin-1β and RANTES were detected in 1 and 3 subjects out of 12 samples. Tumor necrosis factor a was not detected in any of the samples. A significant positive correlation between the levels of MIF and the period with symptoms such as pain, swelling of face, and visual disturbance was observed. Conclusion These findings suggest that MIF and endotoxin may play an important role in the pathogenesis of paranasal sinus mucocele. MIF may be an important factor causing the development and exacerbation of the disease.

scholarly journals The Macrophage Migration Inhibitory Factor Homolog of Entamoeba histolytica Binds to and Immunomodulates Host Macrophages

2014 ◽  
Vol 82 (9) ◽  
pp. 3523-3530 ◽  
Author(s):  
Shannon N. Moonah ◽  
Mayuresh M. Abhyankar ◽  
Rashidul Haque ◽  
William A. Petri
Keyword(s):  
Entamoeba Histolytica ◽  
Migration Inhibitory Factor ◽  
Tissue Damage ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Macrophage Migration ◽  
Content Type ◽  
Amebic Colitis ◽  
Tnf Α ◽  
Wide Range

ABSTRACTThe host inflammatory response contributes to the tissue damage that occurs during amebic colitis, with tumor necrosis factor alpha (TNF-α) being a key mediator of the gut inflammation observed. Mammalian macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that plays an important role in the exacerbation of a wide range of inflammatory diseases, including colitis. We identified a MIF gene homolog in theEntamoeba histolyticagenome, raising the question of whetherE. histolyticaMIF (EhMIF) has proinflammatory activity similar to that of mammalian MIF. In this report, we describe the first functional characterization ofEhMIF. Antibodies were prepared against recombinantly expressedEhMIF and used to demonstrate thatEhMIF is expressed as a 12-kDa protein localized to the cytoplasm of trophozoites. In a manner similar to that of mammalian MIF,EhMIF interacted with the MIF receptor CD74 and bound to macrophages.EhMIF induced interleukin-6 (IL-6) production. In addition,EhMIF enhanced TNF-α secretion by amplifying TNF-α production by lipopolysaccharide (LPS)-stimulated macrophages and by inhibiting the glucocorticoid-mediated suppression of TNF-α secretion.EhMIF was expressed during human infection, as evidenced by the presence of anti-EhMIF antibodies in the sera of children living in an area whereE. histolyticainfection is endemic. Anti-EhMIF antibodies did not cross-react with human MIF. The ability ofEhMIF to modulate host macrophage function may promote an exaggerated proinflammatory immune response and contribute to the tissue damage seen in amebic colitis.

Sign in / Sign up

Export Citation Format

Share Document