PrPSc Is Not Detected in Peripheral Blood Leukocytes of Scrapie-Infected Sheep: Determining the Limit of Sensitivity by Immunohistochemistry
2002 ◽
Vol 9
(2)
◽
pp. 499-502
◽
Keyword(s):
ABSTRACT Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrPSc by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrPSc-positive cells were detected in 2.05% ± 0.28% of 3 × 106 DRLN cells, but were not detected in 3 × 106 PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrPSc-positive cells in 3 × 106 PBLs.
2001 ◽
Vol 82
(6)
◽
pp. 1355-1358
◽
Keyword(s):
Keyword(s):
2018 ◽
Vol 12(21)
(4)
◽
pp. 680-682
Keyword(s):