scholarly journals Jaagsiekte sheep retrovirus can be detected in the peripheral blood during the pre-clinical period of sheep pulmonary adenomatosis

2001 ◽  
Vol 82 (6) ◽  
pp. 1355-1358 ◽  
Author(s):  
L. González ◽  
M. García-Goti ◽  
C. Cousens ◽  
P. Dewar ◽  
N. Cortabarría ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Infected Sheep ◽  
Pathological Examination ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Jaagsiekte Sheep Retrovirus ◽  
Tissue Samples ◽  
The Status ◽  
Pulmonary Adenomatosis ◽  
First Time

Peripheral blood leukocytes (PBLs) and tissue samples from 36 sheep were examined for jaagsiekte sheep retrovirus (JSRV) by hemi-nested PCR. Animals were classified according to the status of sheep pulmonary adenomatosis (SPA), which was confirmed by pathological examination, as follows: (i) sheep with classical SPA (cSPA, n=10), (ii) sheep with atypical SPA (aSPA, n=6), (iii) non-affected sheep from SPA-affected flocks (in-contact, n=10) and (iv) non-affected sheep from SPA-free flocks (control, n=10). JSRV proviral DNA was detected in the PBLs of 10/10 cSPA, 5/6 aSPA, 4/10 in-contact and 0/10 control sheep. Lung tumours and lymphoid organs were also found to be JSRV-positive. The number of positive PCR results was greater for sheep in the cSPA group than for those in the aSPA and in-contact groups. For the first time, it is concluded that JSRV can be detected in naturally infected sheep before the onset of clinical disease and even before the development of discernible tumours.

scholarly journals PrPSc Is Not Detected in Peripheral Blood Leukocytes of Scrapie-Infected Sheep: Determining the Limit of Sensitivity by Immunohistochemistry

2002 ◽  
Vol 9 (2) ◽  
pp. 499-502 ◽  
Author(s):  
Lynn M. Herrmann ◽  
Timothy V. Baszler ◽  
Donald P. Knowles ◽  
William P. Cheevers
Keyword(s):  
Lymph Node ◽  
Peripheral Blood ◽  
Infected Sheep ◽  
Peripheral Blood Leukocytes ◽  
Retropharyngeal Lymph Node ◽  
Blood Leukocytes

ABSTRACT Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrPSc by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrPSc-positive cells were detected in 2.05% ± 0.28% of 3 × 106 DRLN cells, but were not detected in 3 × 106 PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrPSc-positive cells in 3 × 106 PBLs.

scholarly journals Quantification of Global DNA Methylation in Canine Melanotic and Amelanotic Oral Mucosal Melanomas and Peripheral Blood Leukocytes From the Same Patients With OMM: First Study

2021 ◽  
Vol 8 ◽  
Author(s):  
Nayra Villar Scattone ◽  
Tatiane Moreno Ferrarias Epiphanio ◽  
Karine Germano Caddrobi ◽  
Juliana Shimara Pires Ferrão ◽  
Francisco Javier Hernandez-Blazquez ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Peripheral Blood ◽  
High Performance ◽  
Global Dna Methylation ◽  
Image Analysis System ◽  
Peripheral Blood Leukocytes ◽  
Dna Hypomethylation ◽  
Blood Leukocytes ◽  
Tissue Samples ◽  
Analysis System

Oral mucosal melanomas (OMMs) are aggressive and resistant cancers of high importance in veterinary oncology. Amelanotic OMM produces comparatively less melanin and is considered to be more aggressive than melanotic OMM. Global DNA methylation profiles with hypomethylated or hypermethylated patterns have both been associated with aggressive neoplasms; however, global DNA hypomethylation seems to correlate to higher aggressiveness. Accordingly, global DNA methylation in peripheral blood leukocytes has been investigated to understand the role of systemic or environmental factors in cancer development. This study aimed to quantify global DNA methylation in canine melanotic and amelanotic OMM samples and in the peripheral blood leukocytes of the same dogs. Tumor tissue samples were collected from 38 dogs, of which 19 were melanotic and 19 were amelanotic OMM. These were submitted to immunohistochemistry (IHC) with anti-5-methylcytosine (5mC) and anti-Ki67 primary antibodies. Ki67- and 5mC-positive nuclei were manually scored with the help of an image analysis system. Peripheral blood samples were collected from 18 among the 38 OMM-bearing dogs and from 7 additional healthy control dogs. Peripheral blood leukocytes were isolated from the 25 dogs, and DNA was extracted and analyzed by high-performance liquid chromatography (HPLC) for global DNA methylation. The pattern of global DNA methylation in both canine melanotic and amelanotic OMM indicated higher percentages of weakly or negatively stained nuclei in most of the OMM cells, presuming predominant global DNA hypomethylation. In addition, Ki67 counts in amelanotic OMM were significantly higher than those in melanotic OMM (p < 0.001). Global DNA methylation different immunostaining patterns (strong, weak or negative) correlated with Ki67 scores. Global DNA methylation in circulating leukocytes did not differ between the 9 melanotic and 9 amelanotic OMM or between the 18 OMM-bearing dogs and the 7 healthy dogs. This study provides new information on canine melanotic and amelanotic OMM based on global DNA methylation and cell proliferation.

FUNCTIONING OF NO-SYNTHASE IN THE PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH ACNE VULGARIS

2018 ◽  
Vol 14 (66) ◽  
pp. 075
Author(s):  
H. S. Lavryk ◽  
O. P. Korniychuk ◽  
Z. Ya. Fedorovych ◽  
Z. D. Vorobets
Keyword(s):  
Peripheral Blood ◽  
Acne Vulgaris ◽  
No Synthase ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

A Dinucleotide Deletion in the CD24 Gene Is a Potential Risk Factor for Colorectal Cancer

2020 ◽  
Vol 86 (5) ◽  
pp. 480-485
Author(s):  
Lior Segev ◽  
Ilana Naboishchikov ◽  
Diana Kazanov ◽  
Ezra Bernstein ◽  
Meital Shaked ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Peripheral Blood ◽  
Intracellular Signaling ◽  
Genetic Variant ◽  
Colorectal Carcinogenesis ◽  
Polymorphism Analysis ◽  
Peripheral Blood Leukocytes ◽  
Clinical Value ◽  
Blood Leukocytes ◽  
Multistep Process

Background CD24 is a sialoglycoprotein anchored to the cell surface via glycosylphosphatidylinositol and is involved in intracellular signaling processes. It plays an important role in the early stages of the multistep process of colorectal carcinogenesis. Several single nucleotide polymorphisms in the CD24 gene are reported to exert a diverse effect on cancer risk. We aimed to elucidate whether CD24 TG/del genetic variants are associated with susceptibility to colorectal cancer (CRC). Methods The study included 179 subjects, 36 with CRC (prior to surgery) and 143 healthy control subjects. Deoxyribonucleic acid was purified from peripheral blood leukocytes, and by using restriction fragment length polymorphism analysis, the CD24 gene was genotyped for the specific genetic variant, TG deletion. Additionally, CD24 protein expression levels were determined by Western blotting analysis. Results The incidence of the TG/del was higher among the CRC patients compared with healthy controls, 14% and 10%, respectively ( P = .54). CD24 protein levels were significantly higher among CRC patients. There were no significant differences in CD24 expression between CRC patients at different stages of the disease or between patients who carry the mutation and those who did not. Conclusions CD24 genetic variant might be of clinical value for risk assessment as part of cancer prevention programs. Further study on larger populations is needed to validate the importance of this dinucleotide deletion in CRC development. Overexpression of CD24 protein occurs early along the multistep process of CRC carcinogenesis, and a simple blood sample based on CD24 expression on peripheral blood leukocytes can contribute to early diagnosis.

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