scholarly journals Comparative Study of the Standard Fluorescent Antibody to Membrane Antigen (FAMA) Assay and a Flow Cytometry-Adapted FAMA Assay To Assess Immunity to Varicella-Zoster Virus

2011 ◽  
Vol 18 (7) ◽  
pp. 1194-1197 ◽  
Author(s):  
M. M. Lafer ◽  
L. Y. Weckx ◽  
M. I. de Moraes-Pinto ◽  
A. Garretson ◽  
S. P. Steinberg ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Confidence Interval ◽  
Comparative Study ◽  
Varicella Zoster Virus ◽  
Roc Curve ◽  
Operating Characteristic ◽  
Fluorescent Antibody ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Varicella Zoster

ABSTRACTA flow cytometry-adapted fluorescent antibody to membrane antigen (FAMA) assay to detect IgG antibodies against varicella-zoster virus (VZV) was developed and tested in 62 serum samples, showing 90.32% accuracy obtained from a receiver operating characteristic (ROC) curve with a 0.9125 (95% confidence interval [CI], 0.829 to 1.00) area below the curve compared to the result with standard FAMA.

scholarly journals Evaluation of Three Commercial Varicella-Zoster Virus IgG Enzyme-Linked Immunosorbent Assays in Comparison to the Fluorescent-Antibody-to-Membrane-Antigen Test

2012 ◽  
Vol 19 (8) ◽  
pp. 1261-1268 ◽  
Author(s):  
Andreas Sauerbrei ◽  
Anna Schäfler ◽  
Jörg Hofmann ◽  
Michael Schacke ◽  
Bernd Gruhn ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Fluorescent Antibody ◽  
Marrow Transplant ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lower Sensitivity ◽  
Serum Samples ◽  
Reliable Determination ◽  
Whole Cell ◽  
Varicella Zoster ◽  
Cell Enzyme

ABSTRACTCommercial serologic assays for varicella-zoster virus (VZV), which enable reliable determination of VZV immune status and are amenable to automation, are needed. The present study compares the automated performance of the VZV whole-cell enzyme-linked immunosorbent assay (ELISA) Enzygnost anti-VZV/IgG, the Euroimmun anti-VZV ELISA (IgG) based on highly purified viral proteins, and the VZV glycoprotein (gp)-based Serion ELISA Classic VZV IgG. The fluorescent-antibody-to-membrane-antibody (FAMA) test was used as a reference. A total of 638 serum samples from VZV-negative children, blood donors, varicella vaccinees, and bone marrow transplant recipients were included. The Enzygnost anti-VZV/IgG and the Serion ELISA Classic VZV IgG showed sensitivities of 99.6% and 99.2%, respectively, and the Euroimmun anti-VZV ELISA (IgG) had a significantly lower sensitivity of 90.5%. Specificity was calculated as 100% for both the Euroimmun anti-VZV ELISA (IgG) and for the Enzygnost anti-VZV/IgG, and the Serion ELISA Classic VZV IgG had a significantly lower specificity of 89.4%. Quantitative results of all ELISAs correlated well, but there was a poor quantitative correlation between the ELISAs and FAMA. In conclusion, this study does not show any superiority of a gp- and a protein-based ELISA compared to a whole-cell ELISA for the automated detection of VZV-specific IgG. The automated performance of the Enzygnost anti-VZV/IgG assay correlated best with the FAMA reference assay.

scholarly journals Evaluation of Varicella-Zoster Virus Immunoglobulin G in Children With Malignancies on Chemotherapy

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Shiva Nazari ◽  
Mohammad Naderisorki ◽  
Shahnaz Armin ◽  
Zari Gholinejad
Keyword(s):  
Varicella Zoster Virus ◽  
Varicella Vaccine ◽  
Cross Sectional Study ◽  
Lymphocytic Leukemia ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Igg Antibody ◽  
Cross Sectional ◽  
Varicella Zoster ◽  
Underlying Malignancy

Background: Varicella-Zoster Virus (VZV) infection in children with different malignancies on chemotherapy has become an alarming problem. There are insufficient data about VZV seroprevalence among patients in Iran. Objectives: The current study was conducted to assess the seroprevalence of VZV serum IgG antibodies in children with malignancies on chemotherapy. Methods: The current single-center cross-sectional study was conducted from January 2018 to December 2019 at Mofid children’s hospital, Tehran, Iran. Five milliliters of blood samples were collected from the patients. Serum samples were then tested in duplicate for the IgG antibody against VZV using Varicella-Zoster ELISA IgG/IgM kit. Results: A total of 54 children with different malignancies were included. Overall, 24 and 30 serum samples were collected from females and males, respectively. The children were five months to 15-years-old with a mean age of 5.5 years. The overall seroprevalence of VZV IgG in the children was 21.1% (n = 13/54). Of patients with positive VZV IgG antibody, 9 (69.2%) were male, and four (30.8%) were female. Besides, Acute Lymphocytic Leukemia (ALL) with 61.1% (n = 33/54) was the most common underlying malignancy among these patients. The prevalence of anti-VZV IgG antibodies was 21.2% (n = 7/33) in the group of children with ALL. The highest seroprevalence of the VZV IgG antibody was seen in 11 to 15-year-old children (n = 3/7; 42.9%). The prevalence rates of IgG antibodies against VZV among children aged 0-2 years, 3-5 years, and 6-10 years were 28.6%, 25.9%, and 7.7%, respectively. Conclusions: The results showed that children with different malignancies on chemotherapy are quite susceptible to chickenpox infection. It is suggested that a safe and effective live attenuated varicella vaccine in line with the WHO recommendations be incorporated into Iran’s national immunization program for children with various malignancies.

scholarly journals Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Antibody Test for AssayingLeishmania infantumAntibodies in Dogs

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Daniela Proverbio ◽  
Eva Spada ◽  
Luciana Baggiani ◽  
Giada Bagnagatti De Giorgi ◽  
Roberta Perego
Keyword(s):  
Roc Curve ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Elisa Test ◽  
Standard Test ◽  
Confirmatory Test ◽  
Canine Leishmaniosis ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Test Kit

This study compares a rapid Immunospecific Kalazar Canine Rapid Spot IF with the gold standard test (indirect fluorescent antibody test (IFAT)) for detection ofLeishmania infantumspecific IgG serum antibodies in naturally exposed dogs. Serum samples were obtained from 89 healthy dogs and dogs affected by canine leishmaniosis (CanL). IgG-IFAT titers ≥80 were considered positive. Anti-L. infantumIgG antibodies were found in 54 samples with titers ranging from 1 : 80 to 1 : 5120. The performance of the rapid Immunospecific Kalazar was evaluated using a ROC curve. The area under the ROC curve of 0.957 was significantly different from 0.5 (), and therefore it can be concluded that the rapid Immunospecific Kalazar has the ability to distinguish canine sera with and withoutL. infantumIgG. The best performance of the test was at a cutoff >0 (sensitivity 92.6%, specificity 97%). The test can be used for disease screening if the cutoff is >0 (highest sensitivity, 92.6%) and is recommended as confirmatory test for the presence ofL. infantumIgG antibodies if the cutoff is set >2 (highest specificity, 100%).

scholarly journals Intratracheal inoculation of human varicella zoster virus (VZV; MAV strain) vaccine successfully induced VZV IgG antibodies in rhesus monkeys

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Jong-Min Kim ◽  
Chung-Gyu Park
Keyword(s):  
Rhesus Monkey ◽  
Varicella Zoster Virus ◽  
Humoral Immunity ◽  
Antibody Production ◽  
Rhesus Monkeys ◽  
Igg Antibodies ◽  
Simian Varicella Virus ◽  
Varicella Zoster ◽  
Varicella Virus ◽  
Strain Vaccine

Abstract Background The objective of this study was to investigate whether the use of live attenuated varicella zoster virus (VZV) MAV vaccination can efficiently induce VZV antibody production in naive rhesus monkeys as an approach to prevent simian varicella virus (SVV) reactivation in animals immunosuppressed for transplantation studies. Results Clinically available human VZV vaccine was used to induce the production of anti-VZV antibodies in rhesus monkeys. A vial of the vaccine was subcutaneously injected at 0 week, and the second and third vaccination was performed at 5 and 6 weeks by intratracheal inoculation. The titer of anti-VZV IgG was assessed at 0, 2, 4, 6, and 7 weeks. At 2 weeks, 3/16 were seropositive for VZV IgG. At 6 weeks, 9/16 were shown to be seropositive. At 7 weeks, 16/16 were found to be seropositive. Conclusions The VZV vaccine via intratrachael inoculation was shown to induce VZV IgG humoral immunity in rhesus monkeys and may be important immunosuppressed macaques for transplantation studies. Although the humoral immunity produced is an important finding, further studies will be necessary to confirm possible protection and it could protect probably against SVV infection in rhesus monkey.

scholarly journals Herpes zoster recurrence within 1 month: A case report

2021 ◽  
Vol 19 ◽  
pp. 205873922110212
Author(s):  
Nan Zhao ◽  
Yulan Geng ◽  
Yexian Li ◽  
Lijuan Liu ◽  
Yanjia Li ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Case Report ◽  
Herpes Zoster ◽  
B Cells ◽  
Varicella Zoster Virus ◽  
Lower Limit ◽  
Skin Disease ◽  
Clinical Manifestations ◽  
Varicella Zoster ◽  
Humoral Immune

Herpes zoster (HZ), caused by the varicella-zoster virus, is an infectious skin disease that rarely recurs after initial presentation. The mechanism underlying HZ recurrence is currently under investigation. In this article, we report a case of HZ relapse within 1 month. Analysis of patient’s clinical manifestations, histopathological features, and flow cytometry results indicated that the absolute and percentage values of B cells were below the lower limit. We hypothesized that the patient had abnormal humoral immune function, which may be one reason leading to the HZ relapse within 1 month. The findings of this case will serve as useful reference for HZ recurrence for clinicians. This case was impactful and added to the literature on HZ recurrence.

Avoiding a lumbar puncture may be a rash decision: a case report of varicella-zoster virus-associated radiculopathy in advanced HIV infection

2019 ◽  
Vol 30 (10) ◽  
pp. 1031-1033
Author(s):  
Emma Wallis ◽  
Bahij Al-Hakim ◽  
Paul Holmes ◽  
Sam Douthwaite ◽  
Ranjababu Kulasegaram
Keyword(s):  
Varicella Zoster Virus ◽  
Lumbar Puncture ◽  
Case Reports ◽  
Response To Treatment ◽  
High Dose ◽  
Serum Samples ◽  
Cd4 Cell Count ◽  
Varicella Zoster ◽  
Intravenous Acyclovir ◽  
History Of

A 34-year-old man recently diagnosed with advanced human immunodeficiency virus infection (CD4 cell count of 139 cells/mm3), not yet started on antiretroviral medications, presented to hospital with a ten-day history of left leg weakness and difficulty walking. He described a childhood history of chickenpox with previous shingles over his buttock over three years ago. Examination revealed reduced power in the left hip and knee flexors and absent knee and adductor reflexes. Lumbar punctures were performed and polymerase chain reaction (PCR) detected varicella-zoster virus (VZV) DNA. Concurrent serum samples for VZV PCR were negative. The patient was diagnosed with VZV radiculopathy and treated with high-dose intravenous acyclovir. Within two days, neurological signs improved. Previous case reports define VZV radiculopathy by a temporal and geographical relationship with a zoster rash. Our diagnosis was based on a clinical picture of radiculopathy with virological evidence in CSF and confirmed by a dramatic clinical response to treatment. We propose that lumbar puncture and detection of VZV DNA by PCR in the cerebrospinal fluid (CSF) is an invaluable investigation that should be considered in the workup of immunosuppressed patients presenting with a radiculopathy.

scholarly journals Genotypic Analysis of Varicella-Zoster Virus and Its Seroprevalence in Finland

2007 ◽  
Vol 14 (9) ◽  
pp. 1057-1061 ◽  
Author(s):  
Marjaleena Koskiniemi ◽  
Maija Lappalainen ◽  
D. Scott Schmid ◽  
Elena Rubtcova ◽  
Vladimir N. Loparev
Keyword(s):  
Varicella Zoster Virus ◽  
Immunoglobulin G ◽  
Pediatric Patients ◽  
Age Groups ◽  
Wild Type ◽  
Finnish Population ◽  
Serum Samples ◽  
Varicella Zoster ◽  
Genotypic Analysis ◽  
Specific Immunoglobulin

ABSTRACT We evaluated the seroprevalence of varicella-zoster virus (VZV) in the Finnish population among various age groups and genetically characterized VZV strains from documented cases of varicella and zoster. VZV-specific immunoglobulin G was measured in 2,842 serum samples that had been submitted for virological studies to the Department of Virology, University of Helsinki, from 1995 to 1996. Specimens for VZV genotyping were obtained from vesicular lesions from two pediatric patients and 26 adult patients. Seroprevalence to VZV varied markedly by age: 45% in children aged ≤2 months, 12.5% in children aged 6 to 8 months, and >90% in children near 10 years of age, plateauing thereafter into advanced age. The seroprevalence rates indicate that in Finland, as in other countries with temperate climates, primary VZV infection usually occurs during the first decade of life. Twenty-eight VZV DNA-positive specimens were analyzed to identify VZV vaccine and wild-type genotypes. All analyzed specimens were wild type and the European (E) genotype.

scholarly journals Varicella zoster virus in American Samoa: seroprevalence and predictive value of varicella disease history in elementary and college students

2013 ◽  
Vol 142 (5) ◽  
pp. 1002-1007 ◽  
Author(s):  
A. MAHAMUD ◽  
J. LEUNG ◽  
Y. MASUNU-FALEAFAGA ◽  
E. TESHALE ◽  
R. WILLIAMS ◽  
...  
Keyword(s):  
College Students ◽  
Elementary Students ◽  
Varicella Zoster Virus ◽  
American Samoa ◽  
Varicella Vaccination ◽  
Igg Antibodies ◽  
Predictive Values ◽  
Varicella Zoster ◽  
The Us ◽  
History Of

SUMMARYThe epidemiology of varicella is believed to differ between temperate and tropical countries. We conducted a varicella seroprevalence study in elementary and college students in the US territory of American Samoa before introduction of a routine varicella vaccination programme. Sera from 515 elementary and 208 college students were tested for the presence of varicella-zoster virus (VZV) IgG antibodies. VZV seroprevalence increased with age from 76·0% in the 4–6 years group to 97·7% in those aged ⩾23 years. Reported history of varicella disease for elementary students was significantly associated with VZV seropositivity. The positive and negative predictive values of varicella disease history were 93·4% and 36·4%, respectively, in elementary students and 97·6% and 3·0%, respectively, in college students. VZV seroprevalence in this Pacific island appears to be similar to that in temperate countries and suggests endemic VZV circulation.

scholarly journals Evaluation of a Multiplex Bead Immunoassay for Determination of Immune Status to Varicella-Zoster Virus in Medical Center Students and Employees

2015 ◽  
Vol 22 (3) ◽  
pp. 351-353 ◽  
Author(s):  
Michael J. Loeffelholz ◽  
Harry E. Prince
Keyword(s):  
Varicella Zoster Virus ◽  
Enzyme Immunoassay ◽  
Predictive Value ◽  
Immune Status ◽  
Medical Center ◽  
Igg Antibodies ◽  
Varicella Zoster ◽  
Multiplex Bead ◽  
Bead Immunoassay

ABSTRACTThis study evaluated an enzyme immunoassay, a multiplex bead immunoassay (MBIA), and the anticomplement immunofluorescence (ACIF) test for detecting varicella-zoster virus IgG antibodies in sera from medical center students and employees. The agreement between methods was ≥95%. The MBIA was less sensitive than was the ACIF test, with a negative predictive value of 66.7%.

scholarly journals Investigation of an outbreak of varicella in a village of Goalpara District, India

2016 ◽  
Vol 6 (1) ◽  
pp. 93
Author(s):  
Mridul Malakar ◽  
Choudhury Manjit ◽  
Thinsin Sibarani ◽  
Bose Roy Susmita
Keyword(s):  
Varicella Zoster Virus ◽  
Folk Medicine ◽  
Viral Disease ◽  
Health Awareness ◽  
Serum Samples ◽  
District Health ◽  
Blood Samples ◽  
Varicella Zoster ◽  
Traditional Remedies ◽  
Igm Elisa

Background: Chickenpox or varicella is a viral disease caused by varicella-zoster virus (VZV) with different complications. Due to traditional belief peoples/villagers avoids treatment and follows some traditional remedies and folk medicine. To change this thinking of villager’s health awareness is mandatory. The main aim of the study was control the outbreak and minimizes the future transmission.Methods: An outbreak was investigated in a village of Goalpara district of Assam, India after getting the permission from DHS (District Health Society). Total 42 (Forty two) numbers of patients affected out of 953 (Nine hundred fifty three) numbers of villagers. Blood samples were collected and serum obtained from the samples.Results: The serum samples were tested for VZV IgM ELISA and found 88.09 % patients positive for VZV. Hygiene was observed very poor during our investigation.Conclusions: Health awareness given to the all villagers and patients were treated symptomatically. Immunization, Proper nutrition, Improve living behavior etc can reduce the rate of outbreak.

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