Whole-Genome Sequence of the Nitrogen-Fixing Symbiotic Rhizobium
                    Mesorhizobium loti
                    Strain TONO

Mesorhizobium loti is the nitrogen-fixing microsymbiont for legumes of the genus Lotus . Here, we report the whole-genome sequence of a Mesorhizobium loti strain, TONO, which is used as a symbiont for the model legume Lotus japonicus . The whole-genome sequence of the strain TONO will be a solid platform for comparative genomics analyses and for the identification of genes responsible for the symbiotic properties of Mesorhizobium species.

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Draft Genome Sequence of the Nitrogen-Fixing and Hormogonia-Inducing CyanobacteriumNostoc cycadaeStrain WK-1, Isolated from the Coralloid Roots ofCycas revoluta

Genome Announcements ◽

10.1128/genomea.00021-18 ◽

2018 ◽

Vol 6 (7) ◽

Cited By ~ 6

Author(s):

Yu Kanesaki ◽

Masaki Hirose ◽

Yuu Hirose ◽

Takatomo Fujisawa ◽

Yasukazu Nakamura ◽

...

Keyword(s):

Host Plant ◽

Genome Sequence ◽

Draft Genome ◽

Draft Genome Sequence ◽

Whole Genome Sequence ◽

Whole Genome ◽

Nitrogen Fixing ◽

Coralloid Roots ◽

Cycas Revoluta

ABSTRACTWe report here the whole-genome sequence ofNostoc cycadaestrain WK-1, which was isolated from cyanobacterial colonies growing in the coralloid roots of the gymnospermCycas revoluta. It can provide valuable resources to study the mutualistic relationships and the syntrophic metabolisms between the cyanobacterial symbiont and the host plant,C. revoluta.

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Whole-genome sequence, functional annotation, and comparative genomics of the high biofilm-producing multidrug-resistant Pseudomonas aeruginosa MZ4A isolated from clinical waste

Gene Reports ◽

10.1016/j.genrep.2020.100999 ◽

2021 ◽

Vol 22 ◽

pp. 100999

Author(s):

Zulkar Nain ◽

Mohammad Minnatul Karim

Keyword(s):

Pseudomonas Aeruginosa ◽

Comparative Genomics ◽

Genome Sequence ◽

Functional Annotation ◽

Multidrug Resistant ◽

Whole Genome Sequence ◽

Whole Genome

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Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant EnvironmentalStaphylococcus epidermidisST59

G3 Genes|Genome|Genetics ◽

10.1534/g3.118.200314 ◽

2018 ◽

Vol 8 (7) ◽

pp. 2225-2230 ◽

Cited By ~ 7

Author(s):

Zhen Xu ◽

Raju Misra ◽

Dorota Jamrozy ◽

Gavin K. Paterson ◽

Ronald R. Cutler ◽

...

Keyword(s):

Comparative Genomics ◽

Genome Sequence ◽

Whole Genome Sequence ◽

Whole Genome ◽

Drug Resistant ◽

Comparative Genomics Analysis

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Genome Sequence Data of the Soybean Pathogen Stagonosporopsis vannaccii: A Resource for Studies on Didymellaceae Evolution

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-01-20-0016-a ◽

2020 ◽

Vol 33 (8) ◽

pp. 1022-1024

Author(s):

Giovanni Cafà ◽

Thaís Regina Boufleur ◽

Renata Rebellato Linhares de Castro ◽

Nelson Sidnei Massola ◽

Riccardo Baroncelli

Keyword(s):

Comparative Genomics ◽

Genome Sequence ◽

Plant Pathogens ◽

Sequence Data ◽

Gene Prediction ◽

Whole Genome Sequence ◽

Future Research ◽

Whole Genome ◽

Genome Sequence Data ◽

Associated Species

The genus Stagonosporopsis is classified within the Didymellaceae family and has around 40 associated species. Among them, several species are important plant pathogens responsible for significant losses in economically important crops worldwide. Stagonosporopsis vannaccii is a newly described species pathogenic to soybean. Here, we present the draft whole-genome sequence, gene prediction, and annotation of S. vannaccii isolate LFN0148 (also known as IMI 507030). To our knowledge, this is the first genome sequenced of this species and represents a new useful source for future research on fungal comparative genomics studies.

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Genome Sequence of Fusarium graminearum ITEM 124 (ATCC 56091), a Mycotoxigenic Plant Pathogen

Genome Announcements ◽

10.1128/genomea.01209-17 ◽

2017 ◽

Vol 5 (45) ◽

Cited By ~ 5

Author(s):

Antonio Zapparata ◽

Daniele Da Lio ◽

Stefania Somma ◽

Isabel Vicente Muñoz ◽

Luca Malfatti ◽

...

Keyword(s):

Comparative Genomics ◽

Fusarium Graminearum ◽

Crop Yield ◽

Genome Sequence ◽

Whole Genome Sequence ◽

Economic Losses ◽

Whole Genome ◽

Head Blight ◽

Content Type ◽

Yield And Quality

ABSTRACT Fusarium graminearum is among the main causal agents of Fusarium head blight (FHB), or scab, of wheat and other cereals, caused by a complex of Fusarium species, worldwide. Besides causing economic losses in terms of crop yield and quality, F. graminearum poses a severe threat to animal and human health. Here, we present the first draft whole-genome sequence of the mycotoxigenic Fusarium graminearum strain ITEM 124, also providing useful information for comparative genomics studies.

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A Novel Fix¯ Symbiotic Mutant of Lotus japonicus, Ljsym105, Shows Impaired Development and Premature Deterioration of Nodule Infected Cells and Symbiosomes

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-19-0780 ◽

2006 ◽

Vol 19 (7) ◽

pp. 780-788 ◽

Cited By ~ 17

Author(s):

Md. Shakhawat Hossain ◽

Yosuke Umehara ◽

Hiroshi Kouchi

Keyword(s):

Nitrogen Deficiency ◽

Lotus Japonicus ◽

Nodule Development ◽

Acetylene Reduction Activity ◽

Nitrogen Fixing ◽

Wild Type ◽

Electron Microscopic ◽

Model Legume ◽

Mesorhizobium Loti ◽

Infected Cells

Nitrogen-fixing symbiosis between legume plants and rhizobia is established through complex interactions between two symbiotic partners. To identify the host legume genes that play crucial roles in such interactions, we isolated a novel Fix¯ mutant, Ljsym105, from a model legume Lotus japonicus MG-20. The Ljsym105 plants displayed nitrogen-deficiency symptoms after inoculation with Mesorhizobium loti under nitrogen-free conditions, but their growth recovered when supplied with nitrogen-rich nutrients. Ljsym105 was recessive and monogenic and mapped on the upper portion of chromosome 4. The mutant Ljsym105 formed an increased number of small and pale-pink nodules. Nitrogenase (acetylene reduction) activity per nodule fresh weight was low but retained more than 50% of that of the wild-type nodules. Light and electron microscopic observations revealed that the Ljsym105 nodule infected cells were significantly smaller than those of wild-type plants, contained enlarged symbiosomes with multiple bacteroids, and underwent deterioration of the symbiosomes prematurely as well as disintegration of the whole infected cell cytoplasm. These results indicate that the ineffectiveness of the Ljsym105 nodules is primarily due to impaired growth of infected cells accompanied with the premature senescence induced at relatively early stages of nodule development. These symbiotic phenotypes are discussed in respect to possible functions of the LjSym105 locus in the symbiotic interactions required for establishment of the nitrogen-fixing symbiosis.

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A potential genomic recombination site upstream of the rfb locus in Leptospira interrogans is associated with serogroup Serjoe and serovar Hardjo classification

10.1101/771931 ◽

2019 ◽

Author(s):

Maria Raquel V. Cosate ◽

Tetsu Sakamoto ◽

Élvio C. Moreira ◽

José Miguel Ortega ◽

Rômulo C. Leite ◽

...

Keyword(s):

Comparative Genomics ◽

Genome Sequence ◽

Livestock Production ◽

Whole Genome Sequence ◽

Leptospira Interrogans ◽

Whole Genome ◽

Bacterial Strains ◽

Recombination Site ◽

Reproductive Disease ◽

Genomic Recombination

AbstractLeptospirosis is a zoonotic disease caused by pathogenic spirochetes of the genus Leptospira. It has a global distribution and affects domestic animals, including cattle. In livestock production, Leptospira interrogans serogroup Sejroe serovar Hardjo is the major reproductive disease leading to economic losses. The whole-genome sequence of the first Brazilian clinical isolate classified as L. interrogans serogroup Sejroe serovar Hardjo strain Norma enabled the evaluation of its genomic features. Here, we investigated particularities of this isolate, obtained from a leptospirosis outbreak. Bioinformatic analysis using the L. interrogans serovar Hardjo str. Norma was applied as a reference for genomic evaluation and comparative analysis among L. interrogans and L. borgpetersenii serovars. Our data suggest the occurrence of genomic recombination in L. interrogans serovar Hardjo str. Norma encompassing 45 Kb located upstream of the rfb locus. A hallmark of genetic evolution was predicted through an orthologue analysis that identified that sugar enzymes associated with carbohydrate and lipid biosynthesis and metabolism composed this genetic module. Comparative genomics revealed a wide range of relatedness among the bacterial strains of serogroup Sejroe that are classified as L. interrogans and L. borgpetersenii species. Furthermore, identification of an IS3 family suggests a genetic recombination site in L. interrogans serovar Hardjo str. Norma that is distinct among L. interrogans serovars and may contribute to clarify the taxonomic classification of Leptospira spp.Impact StatementLeptospirosis remains an important neglected disease with worldwide distribution. This zoonotic disease impacts in the livestock production and the bovine infection is currently associated to species L. borgpetersenii and L. interrogans serovar Hardjo. L.interrogans serovar Hardjo infection is recognized as reproductive disease associated with abortion and economic lost. In this context, we studied a unique whole genome sequence of L. interrogans serovar Hardjo subtype Hardjo-prajitno isolated from bovine leptospirosis outbreaks in Brazilian dairy farm, one of the greatest country of milk production in world. We compared L. interrogans and L. borgpetersenii genomes with L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjo-prajitno focusing on rfb locus and sugars biosynthesis. Leptospira spp. taxonomy and serology information are strictly associated with rfb locus and we found high correlation among bacterial strains classified in serogroup Sejroe. Although L. interrogans and L. borgpetersenii classified in serogroup Sejroe possess a greater genetic correlation, we uniquely identified that serovar Hardjo strains often possess identical loci carrying predicted sugar biossinthesis genes and mobile elements. The Sru (Sejroe specific Rfb Upstream locus) locus associated to rfb locus probably contribute to Leptospira spp. genetic information concerning serogroup and serovar degrees of taxonomic and serology in this microbiology field.Data SummaryAll Leptospira spp. genome sequences used in this study were retrieved from National Center for Biotechnology Information (NCBI) (Table 1) with NCBI ID: NZ_CP006723.1, NZ_CP012603.1, NC_004342.2, NZ_CP011934.1, NZ_AKXA02000040.1, NZ_CP013147.1, NZ_CP012029.1, NZ_CP015048.1, NC_008508.1, GCA_000216175.3,GCA_000244115.3, NC005823.1, GCA_000244395.3, GCA_000346975.1.HighlightsThe study identified potential molecular features associated with serovar Hardjo subtype Hardjo-prajitno, present in both L. interrogans and L. borgpetersenii, by comparative genomics.A new potential recombinant site found upstream of the rfb locus contains proteins that correlate with serogroup taxonomy in the Leptospira genus.The proteins encoded in the recombinant locus are associated with the synthesis of serological surface determinants such as carbohydrates and lipopolysaccharides.

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