scholarly journals Genome Sequence of a Tomato-Infecting Tomato Mosaic Virus Isolate from Zimbabwe

2018 ◽  
Vol 6 (11) ◽  
Author(s):  
Charles Karavina ◽  
Jacques D. Ibaba ◽  
Augustine Gubba
Keyword(s):  
Next Generation Sequencing ◽  
Mosaic Virus ◽  
Genome Sequence ◽  
Immunosorbent Assay ◽  
Virus Isolate ◽  
De Novo ◽  
Lateral Flow ◽  
Tomato Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Generation Sequencing

ABSTRACT A tomato-infecting tomato mosaic virus (ToMV) isolate was detected in Zimbabwe using lateral flow kits and double-antibody sandwich enzyme-linked immunosorbent assay. Next-generation sequencing and de novo assembly were subsequently performed to determine its genome sequence. The ToMV genome of the Zimbabwe isolate is the second to be reported in Africa.

scholarly journals Genome Sequence and Phylogeny of a Bean Yellow Mosaic Virus Isolate Obtained from a 14-Year-Old Australian Lentil Sample

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
S. Maina ◽  
L. Zheng ◽  
S. King ◽  
M. Aftab ◽  
N. Nancarrow ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Genome Sequence ◽  
Virus Isolate ◽  
De Novo ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Nucleotide Identity ◽  
Australian Isolate ◽  
Content Type ◽  
Western Australian

Using RNA strand-specific sequencing followed by de novo assembly, a Bean yellow mosaic virus (BYMV) genome was obtained from a lentil sample (Aus14BY) collected in Victoria, Australia, in 2005. When compared with 51 BYMV genomes, it closely resembled the Western Australian isolate PN83A (Lupinus angustifolius), with 98.4% nucleotide identity.

Infection of red spruce, black spruce, and balsam fir seedlings with tomato mosaic virus

1992 ◽  
Vol 22 (7) ◽  
pp. 919-924 ◽  
Author(s):  
Volker Jacobi ◽  
John D. Castello
Keyword(s):  
Mosaic Virus ◽  
Immunoelectron Microscopy ◽  
Immunosorbent Assay ◽  
Black Spruce ◽  
Red Spruce ◽  
Balsam Fir ◽  
Tomato Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Spread ◽  
Root Extracts

Red spruce (Picearubens Sarg.), black spruce (Piceamariana (Mill.) B.S.P.), and balsam fir (Abiesbalsamea (L.) Mill.) seedlings were root inoculated with tomato mosaic virus, potted, and maintained in a cold frame. The virus was detected by enzyme-linked immunosorbent assay in root extracts of several inoculated seedlings of each species 6–12 months postinoculation, and in root extracts of approximately 35–40% of both inoculated and noninoculated seedlings of each species 12–18 months postinoculation. Virus spread apparently occurred from the roots of infected to noninfected seedlings within the cold frame. The presence of virus in root extracts was confirmed by immunoelectron microscopy. The virus was not detected in the needles of any seedling at any time by either enzyme-linked immunosorbent assay or immunoelectron microscopy. A transitory needle chlorosis was observed in approximately 14% of the inoculated black spruce and 30% of the inoculated red spruce seedlings, but tomato mosaic virus was not detected in all plants with symptoms.

Complete Genome Sequence of a Novel Wild tomato mosaic virus Isolate Infecting Nicotiana tabacum in China

2015 ◽  
Vol 164 (9) ◽  
pp. 686-690 ◽  
Author(s):  
Hangjun Sun ◽  
Wei Liu ◽  
Jinguang Yang ◽  
Fenglong Wang ◽  
Lili Shen ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virus Isolate ◽  
Tomato Mosaic Virus ◽  
Wild Tomato

scholarly journals Three highly sensitive monoclonal antibody-based serological assays for the detection of tomato mottle mosaic virus

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Xue Li ◽  
Liqian Guo ◽  
Mengmeng Guo ◽  
Duo Qi ◽  
Xueping Zhou ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Large Scale ◽  
Sensitivity Analyses ◽  
Detection Methods ◽  
Tomato Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plant Samples ◽  
Tissue Print ◽  
Dot Elisa

AbstractIn recent years, tomato mottle mosaic virus (ToMMV) has become one of the most important viral pathogens affecting solanaceous crop production in Yunnan, Hainan, and Shandong provinces of China, often causing huge yield reductions. To provide farmers and vegetable industry with reliable and easy-to-use ToMMV detection methods, we immunized BALB/c mice with purified ToMMV and obtained six hybridoma cell lines (i.e., 2D6, 9C12, 26A10, 3A4, 23A4 and 17B11) that secrete anti-ToMMV monoclonal antibodies (MAbs) through the hybridoma technology. Using these MAbs as the detection antibody, we developed three serological assays: antigen-coated-plate enzyme-linked immunosorbent assay (ACP-ELISA), dot enzyme-linked immunosorbent assay (dot-ELISA) and tissue print enzyme-linked immunosorbent assay (tissue print-ELISA) for ToMMV detection. Our test results showed that these three newly developed serological methods can be used to specifically detect ToMMV infection in plant samples, but not tobacco mosaic virus, tomato mosaic virus, cucumber green mottle mosaic virus and cucumber mosaic virus. Sensitivity analyses further showed that ACP-ELISA and dot-ELISA can be used to detect ToMMV infection in plant crude extracts diluted at 1:81,920 and 1:40,960 (weight/volume, g/mL), respectively. Surprisingly, the detection limit of the developed dot-ELISA was 26 times higher than that of traditional RT-PCR. Using field-collected plant samples, we have demonstrated that these three new serological methods are accurate and easy-to-use for large-scale detection of ToMMV in fields.

scholarly journals Susceptibility of Vegetatively Propagated Petunia to Tobamovirus Infection and Its Possible Control

HortScience ◽  
1999 ◽  
Vol 34 (2) ◽  
pp. 292-293 ◽  
Author(s):  
J. Cohen ◽  
Noga Sikron ◽  
S. Shuval ◽  
A. Gera
Keyword(s):  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Phosphate Buffer ◽  
Sodium Phosphate ◽  
Tomato Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Post Inoculation ◽  
Sodium Phosphate Buffer ◽  
Local Lesions ◽  
Systemic Symptoms

In this study, 18 Petunia ×hybrida Hort. Volm.-Andr. cultivars were mechanically inoculated with the tobamoviruses tobacco mosaic (TMV) or tomato mosaic virus (ToMV) (20 μg·L-1 in 0.05 m sodium phosphate buffer). One and 2 weeks post-inoculation (PI), inoculated and noninoculated upper leaves were harvested and assayed for TMV infection using enzyme-linked immunosorbent assay (ELISA). Local lesions developed on inoculated leaves of 16 cultivars 3-5 days PI. A total of 11 and 16 of the cultivars developed systemic symptoms characteristic of tobamovirus infection 2 weeks after inoculation with TMV and ToMV, respectively. All cultivars were positive in ELISA tests. Large amounts of virus were recovered from the upper, noninoculated leaves of all cultivars, including symptomless plants. Up to 95% infection by TMV occurred when a sterilized knife was passed through an infected shoot of petunia prior to its being used to remove cuttings from healthy petunia plants. Heat sterilization of knives and/or treatment with 2.8 g·L-1 sodium troclosene was very effective in controlling TMV transmission.

scholarly journals Complete Genome Sequence of Ornithogalum Mosaic Virus Infecting Gladiolus spp. in South Korea

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Sang-Yun Cho ◽  
Seungmo Lim ◽  
Hongsup Kim ◽  
Seung-In Yi ◽  
Jae Sun Moon
Keyword(s):  
Next Generation Sequencing ◽  
South Korea ◽  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Sanger Sequencing ◽  
Sequence Information ◽  
Next Generation ◽  
Generation Sequencing

We report here the first complete genome sequence of Ornithogalum mosaic virus (OrMV) isolated from Taean, South Korea, in 2011, which was obtained by next-generation sequencing and Sanger sequencing. The sequence information provided here may serve as a potential reference for other OrMV isolates.

Microsatellite loci and the complete mitochondrial DNA sequence characterised through next-generation sequencing and de novo genome assembly, and a preliminary assessment of population genetic structure for the Australian crane, Antigone rubicunda

2019 ◽  
Vol 12 (2) ◽  
pp. 49-58
Author(s):  
Adam D Miller ◽  
Inka Veltheim ◽  
Timothy Nevard ◽  
Han Ming Gan ◽  
Martin Haase
Keyword(s):  
Gene Flow ◽  
Next Generation Sequencing ◽  
Genetic Structure ◽  
Mitochondrial Genome ◽  
Microsatellite Markers ◽  
Genome Sequence ◽  
Population Genetic ◽  
De Novo ◽  
South Eastern ◽  
Generation Sequencing

The Brolga ( Antigone rubicunda) is a large Australian crane species with a broad distribution spanning from the tropical north to the south-eastern regions of the continent. Brolga populations throughout New South Wales, Victoria and South Australia have been in decline since the early twentieth century, with the species being listed as vulnerable in each state. To aid future conservation of the species, its taxonomic status needs to be validated, and patterns of gene flow and population connectivity across the species distribution need to be understood. To assist future genetic studies, we developed a suite of polymorphic microsatellite markers and the complete mitochondrial genome sequence by next-generation sequencing. A total of 18 polymorphic loci were characterised using DNA extractions from 47 individuals, comprising 30 and 17 individuals from Victoria and northern Australia, respectively. We observed moderate genetic variation across loci with only a single locus deviating significantly from Hardy–Weinberg equilibrium. De novo and reference-based genome assemblies were used to assemble the A. rubicunda mitochondrial genome sequence, which consists of 16,700 base pairs, and a typical metazoan mitochondrial gene content and arrangement. We test these new markers by conducting a preliminary analysis of genetic structure between south-eastern and northern Australian Brolga populations. Mitochondrial analyses provided evidence of shared haplotypes across the species range supporting the conspecific status of extant populations, while microsatellite markers indicated weak but significant genetic differentiation suggesting gene flow is limited. We discuss the implications of these findings and the benefits that these genetic markers will provide for future population genetic research on this iconic Australian bird species.

scholarly journals Next-Generation Sequencing Reveals the First Complete Genome Sequence of Cowpea aphid-borne mosaic virus from Uganda

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
E. K. Mbeyagala ◽  
P. Tukamuhabwa ◽  
J. Bisikwa ◽  
T. Holton ◽  
S. B. Mukasa
Keyword(s):  
Next Generation Sequencing ◽  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Indian Isolate ◽  
Next Generation ◽  
Genome Sequences ◽  
Cowpea Aphid ◽  
Generation Sequencing

ABSTRACT We present here the first complete genome sequence of Cowpea aphid-borne mosaic virus (CABMV) isolated from cowpea in Uganda and compare it with five CABMV complete genome sequences from Brazil (2), India (2), and Zimbabwe (1). It most resembled the genomes of two Brazilian isolates (MG-Avr and BR1) and one Indian isolate (RR3).

scholarly journals Several viral diseases of Lycopersicon esculentum circulating in Ukraine

2014 ◽  
Vol 68 (3) ◽  
pp. 96-98
Author(s):  
Al Dalain Emad ◽  
A. Bysov ◽  
O. Shevchenko ◽  
T. Shevchenko ◽  
V. Polischuk
Keyword(s):  
Lycopersicon Esculentum ◽  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Plant Viruses ◽  
Tomato Mosaic Virus ◽  
Viral Diseases ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tomato Plants ◽  
Lycopersicon Esculentum Mill

This paper describes detection of some typical plant viruses infecting Lycopersicon esculentum Mill. plants in Ukraine. Diagnostics using enzyme-linked immunosorbent assay (ELISA) confirmed presence of antigens of viruses belonging to Tobamovirus (PMMoV, ToMV), Cucumovirus (CMV) and Tobravirus (TRV) genera in sap of tomato plants. When studying viral diseases of tomatoes, monoinfection was shown to be prevalent. Tomato mosaic virus (ToMV) was most common.

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