scholarly journals Toward a Complete North American Borrelia miyamotoi Genome

2017 ◽  
Vol 5 (5) ◽  
Author(s):  
Luke C. Kingry ◽  
Adam Replogle ◽  
Dhwani Batra ◽  
Lori A. Rowe ◽  
Christopher Sexton ◽  
...  
Keyword(s):  
Northern Hemisphere ◽  
North American ◽  
Linear Plasmid ◽  
Borrelia Miyamotoi ◽  
Linear Plasmids ◽  
Relapsing Fever ◽  
Draft Sequence ◽  
Content Type ◽  
Human Illness

ABSTRACT Borrelia miyamotoi, of the relapsing-fever spirochete group, is an emerging tick-borne pathogen causing human illness in the northern hemisphere. Here, we present the chromosome, eight extrachromosomal linear plasmids, and a draft sequence for five circular and one linear plasmid of a Borrelia miyamotoi strain isolated from an Ixodes sp. tick from Connecticut, USA.

scholarly journals Blood Smears Have Poor Sensitivity for ConfirmingBorrelia miyamotoiDisease

2019 ◽  
Vol 57 (3) ◽  
Author(s):  
Sam R. Telford ◽  
Heidi K. Goethert ◽  
Philip J. Molloy ◽  
Victor Berardi
Keyword(s):  
Lyme Disease ◽  
Morphological Evidence ◽  
Hard Tick ◽  
Borrelia Miyamotoi ◽  
Relapsing Fever ◽  
Main Mode ◽  
Blood Samples ◽  
Content Type ◽  
Blood Smears ◽  
Thick Smear

ABSTRACTBorrelia miyamotoidisease (BMD) is a newly recognized borreliosis that is cotransmitted by ticks wherever Lyme disease is zoonotic. UnlikeBorrelia burgdorferisensu lato, the agent of Lyme disease,B. miyamotoiis closely related to relapsing fever spirochetes, such asBorrelia hermsii. Some authors have suggested that the disease caused byB. miyamotoishould be considered a hard-tick-transmitted relapsing fever, and thus, the main mode of confirming a diagnosis for that infection, microscopy to analyze a blood smear, may have clinical utility. To determine whether blood smears may detectB. miyamotoiin the blood of acute BMD patients, we made standard malariological thick smears from anticoagulated blood samples that were previously determined to contain this agent (by PCR) and analyzed them for morphological evidence of spirochetes. Spirochetes were not detected in the blood smears from 20 PCR positive patient blood samples after examination of 100 thick smear fields and only 2 of 20 demonstrated spirochetes when the examination was extended to 300 thick smear fields. Inoculation of severe combined immunodeficient (SCID) mice yielded isolates from 5 of 5 samples, but 0 of 3 BALB/c mice became infected. We conclude that in strong contrast to the diagnosis of typical relapsing fever, microscopy of blood smears is not sensitive enough for confirming a diagnosis of BMD but that SCID mouse inoculation could be a useful complement to PCR.

scholarly journals Chromosome and Linear Plasmid Sequences of a 2015 Human Isolate of the Tick-Borne Relapsing Fever Spirochete, Borrelia turicatae

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Luke C. Kingry ◽  
Dhwani Batra ◽  
Adam Replogle ◽  
Christopher Sexton ◽  
Lori Rowe ◽  
...  
Keyword(s):  
Human Isolate ◽  
Open Reading Frames ◽  
Linear Plasmid ◽  
Linear Plasmids ◽  
Relapsing Fever ◽  
Linear Chromosome ◽  
Plasmid Sequence ◽  
Reading Frames

The sequences of the complete linear chromosome and 7 linear plasmids of the relapsing fever spirochete Borrelia turicatae are presented in this report. The 925,547 bp of chromosome and 380,211 bp of plasmid sequence were predicted to contain a total of 1,131 open reading frames, with an average G+C content of 29.7%.

scholarly journals In Vitro Susceptibility of the Relapsing-Fever Spirochete Borrelia miyamotoi to Antimicrobial Agents

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Joris Koetsveld ◽  
Ronald O. P. Draga ◽  
Alex Wagemakers ◽  
Annemijn Manger ◽  
Anneke Oei ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Minimal Bactericidal Concentration ◽  
Hard Tick ◽  
Borrelia Miyamotoi ◽  
Relapsing Fever ◽  
In Vitro Susceptibility ◽  
Content Type ◽  
Microtiter Plates ◽  
Microdilution Method

ABSTRACT Hard-tick-borne relapsing fever (HTBRF) is an emerging infectious disease throughout the temperate zone caused by the relapsing-fever spirochete Borrelia miyamotoi. Antibiotic treatment of HTBRF is empirically based on the treatment of Lyme borreliosis; however, the antibiotic susceptibility of B. miyamotoi has not been studied to date. Thus, we set out to determine the in vitro antimicrobial susceptibility of B. miyamotoi. A microdilution method with 96-well microtiter plates was used to determine the antibiotic susceptibilities of two B. miyamotoi strains isolated on two different continents (Asia and North America), two Borrelia burgdorferi sensu lato strains, and one Borrelia hermsii isolate for purposes of comparison. The MIC and minimal bactericidal concentration (MBC) were determined by both microscopy and colorimetric assays. We were able to show that relative to the B. burgdorferi sensu lato isolates, both B. miyamotoi strains and B. hermsii demonstrated greater susceptibility to doxycycline and azithromycin, equal susceptibility to ceftriaxone, and resistance to amoxicillin in vitro. The MIC and MBC of amoxicillin for B. miyamotoi evaluated by microscopy were 16 to 32 mg/liter and 32 to 128 mg/liter, respectively. Since B. miyamotoi is susceptible to doxycycline, azithromycin, and ceftriaxone in vitro, our data suggest that these antibiotics can be used for the treatment of HTBRF. Oral amoxicillin is currently used as an alternative for the treatment of HTBRF; however, since we found that the B. miyamotoi strains tested were resistant to amoxicillin in vitro, this issue warrants further study.

scholarly journals Chromosome and Large Linear Plasmid Sequences of a Borrelia miyamotoi Strain Isolated from Ixodes pacificus Ticks from California

2017 ◽  
Vol 5 (37) ◽  
Author(s):  
Luke C. Kingry ◽  
Adam Replogle ◽  
Marc Dolan ◽  
Christopher Sexton ◽  
Kerry A. Padgett ◽  
...  
Keyword(s):  
United States ◽  
West Coast ◽  
The United States ◽  
Ixodid Ticks ◽  
Linear Plasmid ◽  
Borrelia Miyamotoi ◽  
Ixodes Pacificus ◽  
The West ◽  
Content Type ◽  
Plasmid Sequence

ABSTRACT Borrelia miyamotoi, a relapsing fever group spirochete, is an emerging tick-borne pathogen. It has been identified in ixodid ticks across the Northern Hemisphere, including the West Coast of the United States. We describe the chromosome and large linear plasmid sequence of a B. miyamotoi isolate cultured from a California field-collected Ixodes pacificus tick.

Foreign aid’s impact on domestic business climates

2016 ◽  
Vol 5 (3) ◽  
pp. 365-382 ◽  
Author(s):  
Nabamita Dutta ◽  
Russell S. Sobel ◽  
Sanjukta Roy
Keyword(s):  
Middle East ◽  
Foreign Aid ◽  
Political Institutions ◽  
North American ◽  
Negative Impact ◽  
Political Elite ◽  
Economic Institutions ◽  
Business Climate ◽  
Government Regulations ◽  
Content Type

Purpose Existing literature has expressed significant pessimism about the outcomes of foreign aid received by developing nations. Foreign aid can lead to negative outcomes by generating greater rent-seeking opportunities and creating aid dependence. While aid’s negative impact has been explored in the context of growth, political institutions, and economic institutions, the literature has not investigated the effect of aid on business climate of recipient nations. The purpose of this paper is to explore foreign aid’s impact on government regulations on the business climate in Sub-Saharan African (SSA) and Middle East and North American countries. Design/methodology/approach The authors consider a panel of 64 countries over six years. Since foreign aid is most likely to be endogenous, as identified in most studies, the identification strategy follows two methodologies – system GMM estimator, that creates its own instruments via moment generating conditions and instrumental variable approach that relies on an external instrument. Findings The authors find that aid worsens the business climate by increasing government restrictions. Foreign aid provides the recipient governments and the political elite resources to strengthen their power and reinforce predatory policies that are harmful for the business climate. The results further show that in the presence of long-lasting and sustainable democratic regimes, the negative impact of foreign aid on business climate mitigates to a certain extent. Originality/value While aid’s negative impact has been explored in the context of growth, political institutions, and economic institutions, the literature has not investigated the effect of aid on business climate of recipient nations. The authors explore the impact of foreign aid on government regulations on the business climate in SSA and Middle East and North American countries.

Dynamic pricing usage in sports for revenue management

2016 ◽  
Vol 42 (9) ◽  
pp. 913-921 ◽  
Author(s):  
Adrien Bouchet ◽  
Michael Troilo ◽  
Brian R. Walkup
Keyword(s):  
North American ◽  
Dynamic Pricing ◽  
Professional Sports ◽  
Business Analytics ◽  
Content Type ◽  
Sports Organizations ◽  
Decision Making Processes ◽  
Sports Leagues ◽  
Job Title ◽  
Travel Services

Purpose The purpose of this paper is to examine the extent to which dynamic pricing is utilized in North American professional sports. While industries such as airlines and travel services have employed dynamic pricing for decades, professional sports is only now starting to adopt it. Design/methodology/approach The authors survey and interview high ranking executives and managers in North American sports organizations. A total of 72 managers and executives from the four major North American professional sports leagues as well as other sport properties were surveyed. Descriptive statistics and a basic regression provide insight into perceptions v. actual practice among sports organizations. Findings While most sports organizations perceive high usage of dynamic pricing within their organization, current procedures lag. Nearly 70 percent of respondents believe that their organizations frequently or always apply business analytics to dynamic pricing, but only 30 percent update their prices daily. Fully 50 percent of organizations do not automate decision-making processes, which is a hallmark of dynamic pricing. The perception of constant use of analytics in dynamic pricing intensifies as job title increases. Originality/value As one of the initial surveys looking at the usage of dynamic pricing in North American professional sports, this study provides a glimpse into both the perception and the reality. It suggests that there is still ample room for improvement.

Linear plasmids in Klebsiella and other Enterobacteriaceae

2021 ◽  
Author(s):  
Jane Hawkey ◽  
Hugh Cottingham ◽  
Alex Tokolyi ◽  
Ryan R Wick ◽  
Louise M Judd ◽  
...  
Keyword(s):  
Plasmid Stability ◽  
Bacterial Species ◽  
Salmonella Typhi ◽  
Multidrug Resistant ◽  
Linear Plasmid ◽  
Extrachromosomal Dna ◽  
Linear Plasmids ◽  
Future Studies ◽  
And Function

Linear plasmids are extrachromosomal DNA that have been found in a small number of bacterial species. To date, the only linear plasmids described in the Enterobacteriaceae family belong to Salmonella, first found in Salmonella Typhi. Here, we describe a collection of 12 isolates of the Klebsiella pneumoniae species complex in which we identified linear plasmids. We used this collection to search public sequence databases and discovered an additional 74 linear plasmid sequences in a variety of Enterobacteriaceae species. Gene content analysis divided these plasmids into five distinct phylogroups, with very few genes shared across more than two phylogroups. The majority of linear plasmid-encoded genes are of unknown function, however each phylogroup carried its own unique toxin-antitoxin system and genes with homology to those encoding the ParAB plasmid stability system. Passage in vitro of the 12 linear plasmid-carrying Klebsiella isolates in our collection (which include representatives of all five phylogroups) indicated that these linear plasmids can be stably maintained, and our data suggest they can transmit between K. pneumoniae strains (including members of globally disseminated multidrug resistant clones) and also between diverse Enterobacteriaceae species. The linear plasmid sequences, and representative isolates harbouring them, are made available as a resource to facilitate future studies on the evolution and function of these novel plasmids.

scholarly journals First Insights into the Genome Sequence of Pseudomonas oleovorans DSM 1045

2017 ◽  
Vol 5 (32) ◽  
Author(s):  
Anja Poehlein ◽  
Rolf Daniel ◽  
Andrea Thürmer ◽  
Alexander Bollinger ◽  
Stephan Thies ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Pseudomonas Oleovorans ◽  
Draft Sequence ◽  
Gram Negative ◽  
Content Type ◽  
Promising Source

ABSTRACT The Gram-negative proteobacterium Pseudomonas oleovorans DSM 1045 is considered a promising source for enzymes of biotechnological interest, e.g., hydrolases and transaminases. Here, we present a draft sequence of its 4.86-Mb genome, enabling the identification of novel biocatalysts.

scholarly journals Detection of Borrelia burgdorferi Sensu Lato and Relapsing Fever Borrelia in Feeding Ixodes Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of Borrelia yangtzensis and Borrelia miyamotoi

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 846
Author(s):  
Alice C. C. Lau ◽  
Yongjin Qiu ◽  
Mohamed Abdallah Mohamed Moustafa ◽  
Ryo Nakao ◽  
Michito Shimozuru ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Primary Forest ◽  
Borrelia Miyamotoi ◽  
Relapsing Fever ◽  
Ixodes Ticks ◽  
Ixodes Granulatus ◽  
New Geographical Record ◽  
Etiological Agents ◽  
Malaysian Borneo

Members of the Borrelia burgdorferi sensu lato (Bbsl) complex are etiological agents of Lyme disease (LD), and Borrelia miyamotoi is one of the relapsing fever Borrelia (RFB). Despite the serological evidence of LD in Malaysia, there has been no report from Sarawak, Malaysian Borneo. Thus, this study aimed to detect and characterize Borrelia in rodents and Ixodes ticks from primary forests and an oil palm (OP) plantation in Sarawak. Borrelia yangtzensis (a member of the Bbsl complex) was detected in 43.8% (14/32) of Ixodes granulatus; most of the positive ticks were from the OP plantation (13/14). Out of 56 rodents, B. yangtzensis was detected in four Rattus spp. from the OP plantation and B. miyamotoi was detected in one rodent, Sundamys muelleri, from the primary forest. Further, the positive samples of B. yangtzensis were randomly selected for multilocus sequence analysis (MLSA). The MLSA results of successfully amplified tick samples revealed a clustering with the sequences isolated from Japan and China. This study is the first evidence of B. miyamotoi, a known human pathogen in Malaysia, and B. yangtzensis, which is circulating in ticks and rodents in Sarawak, Malaysian Borneo, and presenting a new geographical record of the Borrelia spp.

scholarly journals Draft Genome Sequence of an Indian Marine Cyanobacterial Strain with Fast Growth and High Polyglucan Content

2017 ◽  
Vol 5 (47) ◽  
Author(s):  
Ruchi Pathania ◽  
Ahmad Ahmad ◽  
Shireesh Srivastava
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Fast Growth ◽  
Marine Cyanobacteria ◽  
Cyanobacterial Strain ◽  
Draft Sequence ◽  
Marine Cyanobacterium ◽  
Content Type ◽  
Potential Source ◽  
Global Carbon

ABSTRACT Marine cyanobacteria play an important role in global carbon cycling and are a potential source of polyglucans for biotechnological purposes. This report provides the draft sequence of an Indian marine cyanobacterium, Synechococcus elongatus BDU 130192, which shows fast growth and high polyglucan content. The genome sequence will help in understanding the unique properties of this organism.

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