scholarly journals Effect of sucrose in culture media on the location of glucosyltransferase of Streptococcus mutans and cell adherence to glass surfaces.

1978 ◽  
Vol 20 (3) ◽  
pp. 592-599 ◽  
Author(s):  
S Hamada ◽  
M Torii
Keyword(s):  
Streptococcus Mutans ◽  
Culture Media ◽  
Cell Adherence ◽  
Glass Surfaces

Inhibition of Adherence of Streptococcus mutans to Glass Surfaces

1977 ◽  
Vol 11 (3) ◽  
pp. 153-159 ◽  
Author(s):  
E. Newbrun ◽  
F. Finzen ◽  
M. Sharma
Keyword(s):  
Streptococcus Mutans ◽  
Glass Surfaces

scholarly journals CLONAL DIVERSITY OF STREPTOCOCCUS MUTANS CLARKE (1924) IN CARIES-FREE ADULTS

2005 ◽  
Vol 27 (58) ◽  
Author(s):  
Rosimeire Takaki Rosa ◽  
Marcelo Henrique Napimoga ◽  
José Francisco Höfling ◽  
Reginaldo Bruno Gonçalves ◽  
Edvaldo Antonio Ribeiro Rosa
Keyword(s):  
Streptococcus Mutans ◽  
Protein Extraction ◽  
Culture Media ◽  
Clonal Diversity ◽  
Mutans Streptococci ◽  
Cell Protein ◽  
Enzyme Electrophoresis ◽  
Dental Biofilm ◽  
Whole Cell Protein ◽  
Microbial Samples

Very few information about the clonal profile of Streptococcus mutans in caries-free adults is available. Microbial samples of saliva, tongue dorsum, and dental biofilm were taken from eight caries-free volunteers (DMFT = 0). Presumptive colonies of mutans streptococci were identified until species level. After confirmation, S. mutans strains were grown in liquid culture media and harvested for whole-cell protein extraction. Multilocus Enzyme Electrophoresis (MLEE) separated protein extracts and the bands’ patterns were assessed for six enzymes. Results showed that two or more distinct clonal types (avg = 4.75) were found cocolonizing all the enrolled subjects, confirming the premise that caries-free subjects are prone to be colonized by more than one strain of S. mutans.

scholarly journals Effect of Streptococcus mutans on surface-topography, microhardness, and mechanical properties of contemporary resin composites

2021 ◽  
Vol 19 ◽  
pp. 228080002110652
Author(s):  
América Monserrat Rios-Madrigal ◽  
Dulce Carolina Orea-Vega ◽  
Marina Vega-González ◽  
León Francisco Espinosa-Cristóbal ◽  
Ma. Concepción Arenas-Arrocena ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Streptococcus Mutans ◽  
Culture Media ◽  
Oral Bacteria ◽  
Resin Composites ◽  
Flexure Strength ◽  
Prevalent Disease ◽  
Diametral Tensile Strength ◽  
Scanning Electron ◽  
Surface Changes

Objective: Dental caries is the most prevalent disease globally, and Streptococcus mutans ( S. mutans) is a common associated oral bacteria. Additionally, S. mutans possess esterase activity capable of degrading resin composites (RC). However, the effect of degradation on the physical-mechanical properties of the RC has not been extensively studied. We evaluated the flexure strength (FS), the diametral tensile strength (DTS), the modulus of elasticity (ME), and the microhardness of three contemporary RC to establish if S. mutans could affect them. Methods: One hundred thirty-eight bar-shaped and 276 disc-shaped specimens were fabricated with Enamel Plus HRi, IPS Empress Direct, and Clearfil AP-X, and physical-mechanical testing was done after been incubated during 30 and 60 days in culture media with or without S. mutans. Also, a scanning electron microscope was used to identify surface changes. Results: None of the tested RC were affected in their mechanical properties (FS, ME, and DTS). However, Clearfil AP-X and Enamel Plus HRI showed eroded surfaces and a decreased microhardness after 30 and 60 days S. mutans incubation. IPS Empress Direct presented the lowest values in all the tests, but its physical-mechanical features and surface were not affected by bacteria’s exposure. Conclusions: Exposure to S. mutans could affect some contemporary RC; however, the effect seems superficial since its mechanical features were not affected.

scholarly journals Antibodies against dextransucrase from Streptococcus mutans display anti-biofilm and growth suppressing activities

2021 ◽  
Author(s):  
Shabeer Ahmad Rather ◽  
Akhtar Mahmood ◽  
Lakhvinder Singh ◽  
Alka Bhatia ◽  
Sukesh Chander Sharma ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Membrane Fraction ◽  
Inhibitory Effect ◽  
Cross Reactivity ◽  
Plaque Formation ◽  
Hydrophobic Properties ◽  
Experimental Conditions ◽  
Glass Surfaces ◽  
Reduced Adherence

Abstract Dextransucrase produced by Streptococcus mutans play an essential role in the formation of dental caries by synthesizing exopolysaccharides from sucrose, an important metabolite of the organism. In this study we report the location of dextransucrase in Streptococcus mutans cells and describe that antibodies raised against dextransucrase inhibited biofilm formation and reduced the adherence and hydrophobic properties of Streptococcus mutans. Western blot analysis and immunoelectron microscopy revealed that dextransucrase is located abundantly in the membrane fraction in S. mutans cells. Scanning electron microscopy and fluorescence microscopy revealed reduced cell density, impaired bioflim (plaque) formation in presence of dextransucrase antibodies. Genes associated with bioflim formation in S. mutans such as GtfB, GtfC, BrpA, relA, Smu630, vicK were down regulated (50–97%) in presence of the enzyme antibody. Presence of enzyme antibodies reduced adherence of S. mutans cells to glass surfaces by 58% and hydrophobicity by 55.2%. However dextransucrase antibodies did not affect acid production by S. mutans, under the experimental conditions. Immunohistochemistry studies with certain human samples displayed no cross reactivity with dextransucrase antibody. These findings suggest that antibodies against dextransucrase exhibit a profound inhibitory effect on the vital cariogenic factors of S. mutans and have no cross reactivity with human tissues tested, thus implying that dextransucrase could be a promising antigen to study its anticariogenic potential.

scholarly journals Antimicrobial Photodynamic Therapy Protocols on Streptococcus mutans with Different Combinations of Wavelengths and Photosensitizing Dyes

2019 ◽  
Vol 6 (2) ◽  
pp. 42
Author(s):  
Elisabetta Merigo ◽  
Stefania Conti ◽  
Tecla Ciociola ◽  
Maddalena Manfredi ◽  
Paolo Vescovi ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Diode Laser ◽  
Streptococcus Mutans ◽  
Toluidine Blue ◽  
Culture Media ◽  
Bactericidal Effect ◽  
In Vivo Studies ◽  
Blue Dye ◽  
532 Nm

The aim of the study is to test the application of different laser wavelengths, with and without different photosensitizing dyes on different types of cultures. Laser irradiation was realized on Streptococcus mutans in both solid and liquid culture media in continuous mode at three different fluences (10, 20, and 30 J/cm2) with a red diode (650 nm) with toluidine blue dye, a blue-violet diode (405 nm) with curcumin dye, and a green diode (532 nm) with erythrosine dye. Without a photosensitizer, no growth inhibition was obtained with the red diode at any fluence value. Inhibition rates of 40.7% and 40.2% were obtained with the blue diode and green diode. The blue diode laser used with curcumin obtained results in terms of growth inhibition up to 99.26% at a fluence of 30 J/cm2. The red diode laser used with toluidine blue obtained results in terms of growth inhibition up to 100% at fluences of 20 and 30 J/cm2. The KTP (potassium-titanyl-phosphate) laser used with erythrosine was able to determine a complete growth inhibition (100%) at the different fluence values. The combination of a laser and its proper color may dramatically change the results in terms of bactericidal effect. It will be interesting to confirm these data by further in vivo studies.

Effect of Trace Elements on Dissolution of Hydroxyapatite by Cariogenic Streptococci

1975 ◽  
Vol 54 (6) ◽  
pp. 1107-1114 ◽  
Author(s):  
Richard J. Herbison ◽  
Stanley L. Handelman
Keyword(s):  
Trace Elements ◽  
Streptococcus Mutans ◽  
Bacterial Growth ◽  
Acid Production ◽  
Culture Media ◽  
Broth Culture ◽  
Synthetic Hydroxyapatite ◽  
Low Levels

The effect of low levels of strontium, boron, lithium, molybdenum, and fluorine, alone and in combination, on hydroxyapatite solubility, bacterial growth, and acid production in five antigenic types of Streptococcus mutans was investigated. Pour Plates containing synthetic hydroxyapatite were used to compare dissolution of hydroxyapatite. The colonies of the five antigenic types of S mutans produced zones of dissolution that were measured. Acid production and growth were studied in broth culture media. The results show that low levels of strontium and fluorine can significantly reduce demineralization of synthetic hydroxyapatite by S mutans in vitro.

Effect of the Probiotic Lactobacillus rhamnosus LB21 on the Cariogenicity of Streptococcus mutans UA159 in a Dual-Species Biofilm Model

2015 ◽  
Vol 49 (6) ◽  
pp. 583-590 ◽  
Author(s):  
Constanza E. Fernández ◽  
Rodrigo A. Giacaman ◽  
Livia M. Tenuta ◽  
Jaime A. Cury
Keyword(s):  
Streptococcus Mutans ◽  
Culture Media ◽  
Lactobacillus Rhamnosus ◽  
Surface Hardness ◽  
Single Species ◽  
Initial Inoculum ◽  
Biofilm Model ◽  
Caries Model ◽  
Bovine Enamel

Despite promising results using probiotics, evidence of the preventive effect on enamel demineralization is insufficient and the cariogenic potential of probiotics is still controversial. Probiotics could affect biofilm formation and interfere with adherence, growth or coaggregation with Streptococcus mutans in biofilms. However, most of the studies have been conducted using planktonic bacteria. Hence, the aim of the study was to assess the effect of probiotic bacteria on the cariogenicity of S. mutans using an in vitro biofilm caries model on enamel. Single-species biofilms (S. mutans UA159, SM or Lactobacillus rhamnosus LB21, LB) or dual-species biofilms simultaneously inoculated (SM + LB) or LB inoculated 8 h after SM (SM → LB) were grown for 96 h. Biofilms were formed on bovine enamel saliva-coated slabs of known surface hardness (SH) and immersed in culture media. Biofilms were exposed 8 times per day to 10% sucrose. Medium pH was monitored twice daily as a biofilm acidogenicity indicator. After 96 h, biofilms were collected to determine biomass and bacteria viability. Slab demineralization was calculated as percentage of SH loss (%SHL). Additionally, the model was tested with different concentrations of the initial inoculum (103, 106, 108 cells/ml) and different adhesion times (2 or 8 h). The dual-species biofilm revealed no LB effects on SM cariogenicity, without changes in acidogenicity or %SHL among groups (p > 0.05, n = 12). Lack of activity of LB on SM cariogenicity persisted even when 105 times higher concentration of the probiotic was tested. Coaggregation was not observed. In conclusion, findings suggest that LB does not reduce cariogenicity of SM in a validated experimental caries model.

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