Modification of Sucrose Dependent Cell Adherence by Deletion and Reintroduction of the gtf Genes in Streptococcus mutans

1997 ◽  
pp. 665-668 ◽  
Author(s):  
Makoto Tamesada ◽  
Shigetada Kawabata ◽  
Zhuan Bian ◽  
Taku Fujiwara ◽  
Shigenobu Kimura ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Cell Adherence ◽  
Dependent Cell

scholarly journals Sucrose-dependent Cell Adherence and Cariogenicity of Serotype c Streptococcus mutans

Microbiology ◽  
1986 ◽  
Vol 132 (10) ◽  
pp. 2873-2883 ◽  
Author(s):  
T. KOGA ◽  
H. ASAKAWA ◽  
N. OKAHASHI ◽  
S. HAMADA
Keyword(s):  
Streptococcus Mutans ◽  
Cell Adherence ◽  
Dependent Cell

Identification and characterization of a polysaccharide-containing antigen fromSchistosoma mansonieggs which cross-reacts with the surface of schistosomula

Parasitology ◽  
1987 ◽  
Vol 94 (2) ◽  
pp. 255-268 ◽  
Author(s):  
D. W. Dunne ◽  
Q. D. Bickle
Keyword(s):  
Molecular Weight ◽  
Schistosoma Mansoni ◽  
High Molecular Weight ◽  
Concanavalin A ◽  
Trichloroacetic Acid ◽  
Soluble Fraction ◽  
Cell Adherence ◽  
Dependent Cell ◽  
Identification And Characterization

SUMMARYAntisera were produced by immunizing rabbits with either a trichloroacetic acid-soluble fraction, or a high molecular weight (Mr) fraction ofSchistosoma mansoniSEA (a salinesoluble fraction of homogenized egg). Both of these sera reacted monospecifically in immunoelectrophoresis against unfractionated SEA, recognizing a cathodally migrating antigen. This antigen had been identified previously as being responded to byS. mansoni-infected mouse sera, and has been designated K3(Kappa 3). The rabbit antisera were used to partially characterize antigen K3as havingMrin the range > 750 – 70 K, and being resistant to boiling, resistant to the action of proteases, but sensitive to periodate. It partially binds to Concanavalin A. In addition to SEA, the antigen was present in homogenized cercariae and schistosomula, but not adult worms, and it was also present in detergent extracts of intact cercariae and schistosomula. Using an antibody-dependent cell adherence assay, anti-K3serum was found to react with the surface of live cercariae and with the surface of schistosomula recovered from the skin of mice infected up to 48 h previously. Anti-K3serum also reacted with the surface ofS. bovis, S. haematobiumand to a lesser extentS. japonicumschistosomula.

scholarly journals Isolation and Characterisation of Probiotics for Antagonising Cariogenic Bacterium Streptococcus mutans and Preventing Biofilm Formation

2016 ◽  
Author(s):  
Pei-Pei Lin ◽  
You-Miin Hsieh ◽  
Cheng-Chih Tsai
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Antimicrobial Properties ◽  
Antagonistic Activity ◽  
Lactobacillus Pentosus ◽  
Chewing Gums ◽  
Dependent Cell ◽  
Cariogenic Bacterium

Consumption of refined foods and beverages high in sugar make the teeth susceptible to the formation of biofilm, and lead to dental caries and diseases of the oral cavity such as periodontitis and periodontal disease. The aim of the present study was to determine the ability of selected probiotics to inhibit growth and biofilm formation by the cariogenic bacterium Streptococcus mutans in vitro. We screened strains of latic acid bacteria (LAB) (n=120) from the Bioresources Collection and Research Center (BCRC), saliva of healthy adults and infant stool. The antimicrobial activity of LAB in vitro was evaluated by agar spot culture and co-culture of the S. mutans strains. We determined the effect of heating and dilution factors (2- and 4-fold) on the antagonistic activity of LAB. Antagonistic substances in the spent culture suspensions (SCS) of LAB were precipitated by extraction with ammonium sulphate and chloroform to characterise the protein and lipophilic fractions. Results of co-culturing show that the SCS of the three LAB strains (Lactobacillus pentosus 13-1, 13-4 and Lactobacillus crispatus BCRC 14618) subjected to heat treatment showed significantly high antimicrobial activity. We found that substances produced by L. pentosus 13-4 which have the potential to exhibit antimicrobial properties might be lipophilic proteins. Additionally, we infer that the mechanism of reducing biofilm formation by Lactobacillus strains is associated with sucrose-dependent cell–cell adhesion and the gtfC level of glucosyltransferases (Gtfs) in the biofilm. Native LAB strains screened in our study may be used in chewing gums and other processed foods for preventing tooth decay.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

1971 ◽  
Vol 29 ◽  
pp. 338-339
Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall
Keyword(s):  
Cell Wall ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Genetic Heterogeneity ◽  
Morphological Characteristics ◽  
Electron Microscopic ◽  
Dna Reassociation ◽  
Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

The role of intracanal medicaments in inhibition of bacteria isolated from root canals of infected primary molars

2019 ◽  
Vol 14 (1) ◽  
pp. 92
Author(s):  
Dr. Maha Abdul- Kareem Mahmood ◽  
Dr. Huda Elias Ali ◽  
Dr. Haraa Khairi Abdul-Kadher
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Normal Saline ◽  
Root Canal ◽  
Primary Teeth ◽  
Primary Molars ◽  
Root Canals ◽  
Etiologic Agents ◽  
Micro Organisms

Microbes are considered as the primary etiologic agents in endodontic diseases.Disinfection of the root canal is obtained by the combined effect of biomechanicalpreparation, irrigation and intra canal medicament. The aim of the present study wasto assess the antimicrobial activity of intracanal medicaments (formocresol andEndosepton) against two micro organisms (Streptococcus mutans and staphylococcusaureus) isolated from 15 necrotic pulps of primary molars indicated for pulpectomyprocedure. The samples were cultured, and purified using microbiological evaluation.Broth dilution test was performed in our study by preparing test tubes containing10 ml of BHI broth (pH. 7) which then inoculated with strains of the tested bacteriaand incubated at 37 C° for 24 h. After over night incubaction, ten fold dilution weremade in test tubes containing 9 ml of normal saline by adding 1 ml of the inoculum tothe first tube . Then from dilution 10-1 , 0.1 ml of cell suspension was added to 9.9 mlof formocresol and endosepton, then 0.1 ml was taken and spread on duplicates ofBHI agar plates at different intervals and incubated aerobically for 24 h. at 37 C°.Colonies on the plates were counted after incubation and CFU/mL (colony formingunit) was calculated. Our results indicating that there were no significant differencesbetween the intracanal medicaments, but there were high significant differencesbetween the intervals time of the study. We concluded that both materials had greatantibacterial effect against the pathogens commonly isolated from necrotic pulpaltissue of primary teeth.

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