scholarly journals Comparison of the Novel Partec Rapid Malaria Test to the Conventional Giemsa Stain and the Gold Standard Real-Time PCR

2010 ◽  
Vol 48 (8) ◽  
pp. 2925-2928 ◽  
Author(s):  
B. Nkrumah ◽  
A. Agyekum ◽  
S. E. K. Acquah ◽  
J. May ◽  
E. Tannich ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Gold Standard ◽  
Giemsa Stain ◽  
The Novel ◽  
Malaria Test

Real-time PCR versus viral culture on urine as a gold standard in the diagnosis of congenital cytomegalovirus infection

2012 ◽  
Vol 53 (2) ◽  
pp. 167-170 ◽  
Author(s):  
Jutte J.C. de Vries ◽  
Annemiek A. van der Eijk ◽  
Katja C. Wolthers ◽  
Lisette G. Rusman ◽  
Suzan D. Pas ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Gold Standard ◽  
Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
Viral Culture

Latent class analysis for the diagnosis of Clostridioides difficile infection

2020 ◽  
Author(s):  
Cody P Doolan ◽  
Thomas Louie ◽  
Christopher Lata ◽  
Oscar E Larios ◽  
William Stokes ◽  
...  
Keyword(s):  
Latent Class Analysis ◽  
Real Time ◽  
Clinical Diagnosis ◽  
Real Time Pcr ◽  
Gold Standard ◽  
Latent Class ◽  
Fecal Calprotectin ◽  
Clostridioides Difficile ◽  
Toxigenic Culture ◽  
Clostridioides Difficile Infection

Abstract Background Clostridioides difficile infection (CDI) is an opportunistic disease that lacks a gold standard test. Nucleic acid amplification tests (NAATs) such as real-time PCR demonstrate excellent an limit of detection (LOD) whereas antigenic methods are able to detect free toxin. Latent class analysis (LCA) provides an unbiased statistical approach to resolving true disease. Methods A cross-sectional study was conducted with suspected CDI patients (n=96). Four commercial real-time PCR tests, toxin antigen detection by enzyme immunoassay (EIA), toxigenic culture, and fecal calprotectin were performed. CDI clinical diagnosis was determined by consensus majority of three experts. LCA was performed using laboratory and clinical variables independent of any gold standard. Results Six LCA models were generated to determine CDI probability using four variables including toxin EIA, toxigenic culture, clinical diagnosis, and fecal calprotectin levels. Three defined zones as a function of real-time PCR cycle threshold (Ct) were identified using LCA: CDI likely (>90% probability), equivocal (<90% and >10%), CDI unlikely (<10%). A single model comprising toxigenic culture, clinical diagnosis, and toxin EIA showed the best fitness. The following Ct cut-offs for four commercial test platforms were obtained using this model to delineate three CDI probability zones: [GeneXpert ® : 24.00, 33.61], [Simplexa ® 28.97, 36.85], [Elite MGB ® 30.18, 37.43], and [BD Max ™ 27.60, 34.26]. Conclusion The clinical implication of applying LCA to CDI is to report Ct values assigned to probability zones based on the commercial real-time PCR platform. A broad range of equivocation suggests clinical judgement is essential to the confirmation of CDI.

scholarly journals Test for hepatitis B virus infection with radical immunoassay and real-time PCR: Which method is the gold standard?

2009 ◽  
Vol 50 (1) ◽  
pp. 211-212
Author(s):  
Shi-Ying Xuan ◽  
Yong-Ning Xin ◽  
Yan-Dan Zhong ◽  
Ming-Hua Zheng ◽  
Hua-Shi Guan
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Gold Standard ◽  
Hepatitis B Virus Infection ◽  
B Virus

scholarly journals Real-time PCR to supplement gold-standard culture-based detection of Legionella in environmental samples

2015 ◽  
Vol 119 (4) ◽  
pp. 1158-1169 ◽  
Author(s):  
S. Collins ◽  
F. Jorgensen ◽  
C. Willis ◽  
J. Walker
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Gold Standard ◽  
Environmental Samples ◽  
Standard Culture

scholarly journals Confirmation of multi-parallel quantitative real-time PCR as the gold standard for detecting soil-transmitted helminths in stool

2021 ◽  
Author(s):  
Marina Papaiakovou ◽  
Nils Pilotte ◽  
Julia Dunn ◽  
David TJ Littlewood ◽  
Rubén O Cimino ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Gold Standard ◽  
Low Cost ◽  
Strongyloides Stercoralis ◽  
Diagnostic Methods ◽  
Quantitative Real Time Pcr ◽  
Soil Transmitted Helminths ◽  
Stool Samples ◽  
Kappa Agreement

AbstractDue to its simplicity and cost-effectiveness, microscopy has seen extensive field-use as the diagnostic standard for the detection of soil-transmitted helminths (STH) in stool samples. However, the sensitivity of microscopy-based detection is inadequate in reduced-transmission settings where worm burden is oftentimes low. Equally problematic, eggs of closely related species oftentimes have indistinguishable morphologies, leading to species misidentification. In light of these shortcomings, the purpose of this study was to demonstrate multi-parallel quantitative real-time PCR (qPCR) as the new “gold standard” for STH detection. Accordingly, stool samples from non-endemic participants were spiked with limited numbers of eggs or larvae (1 to 40) of five different species of STH. DNA extracts were tested using two unique multi-parallel real-time PCR-based diagnostic methods. These methods employed different target sequences (ribosomal internal transcribed spacer, or highly repetitive non-coding regions), to evaluate the detection of DNA from as little as one egg per sample. There was a statistically significant kendall correlation between egg/larvae counts and qPCR from both methods for Trichuris trichiura (0.86 and 0.872 for NHM and Baylor assays) and a strong correlation (0.602 and 0.631 for NHM and Baylor assays, respectively) for Ascaris lumbricoides. Less strong but still significant was the Kendall Tau-b value for A. duodenale (0.408 for both) and for S. stercoralis (0.483 and 0.653, respectively). In addition, using field stool samples from rural Argentina both assays had fair to moderate kappa agreement (0.329-0.454), except for Strongyloides stercoralis (0.121) that both assays had slight agreement. In spite of the small cohort of samples, both qPCR assays, targeting of two independent genomic regions, provided reproducible results and we believe that, low cost multi-parallel quantitative real-time PCR-based diagnostics should supplant microscopy as the new gold standard for stool-based detection of soil transmitted helminths in public-health and community settings.

scholarly journals Performance comparison of two malaria rapid diagnostic test with real time polymerase chain reaction and gold standard of microscopy detection method

2020 ◽  
Author(s):  
Puspa Wardhani ◽  
Trieva Verawaty Butarbutar ◽  
Christophorus Oetama Adiatmaja ◽  
Amarensi Milka Betaubun ◽  
Nur Hamidah ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Diagnostic Test ◽  
Real Time Pcr ◽  
Predictive Value ◽  
Gold Standard ◽  
Detection Method ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Background: The diagnostic test for malaria is mostly based on Rapid Diagnostic Test (RDT) and detection by microscopy. Polymerase Chain Reaction (PCR) is also a sensitive detection method that can be considered as a diagnostic tool. The outcome of malaria microscopy detection depends on the examiner's ability and experience. Some RDT has been distributed in Indonesia, which needs to be evaluated for their results. Objective: This study aimed to compare the performance of RightSign RDT and ScreenPlus RDT for detection of Plasmodium in human blood. We used specific real-time polymerase chain reaction abTESTMMalaria qPCRII) and gold standard of microscopy detection method to measure diagnostic efficiency. Methods: Blood specimens were evaluated using RightSign RDT, ScreenPlus RDT, Microscopy detection, and RT-PCR as the protocol described. The differences on specificity (Sp), sensitivity (Sn), positive predictive value (PPV), and negative predictive value (NPV) were analyzed using McNemar and Kruskal Wallis analysis. Results: A total of 105 subjects were recruited. Based on microscopy test, RightSign RDT had sensitivity, Specificity, PPV, NPV, 100%, 98%, 98.2%, 100%, respectively. ScreenPlus showed 100% sensitivity, 98% specificity, 98.2% PPV, 100% NPV. The sensitivity of both RDTs became lower (75%) and the specificity higher (100 %) when using real-time PCR. Both RDTs showed a 100% agreement. RT-PCR detected higher mix infection when compared to microscopy and RDTs. Conclusion: RightSign and ScreenPlus RDT have excellent performance when using microscopy detection as a gold standard. Real-time PCR method can be considered as a confirmation tool for malaria diagnosis.

scholarly journals VP06.04: The novel screening method based on real‐time PCR for common fetal trisomies

2020 ◽  
Vol 56 (S1) ◽  
pp. 76-76
Author(s):  
J. Park ◽  
S. Kim ◽  
S. Lee ◽  
B. Kim ◽  
J. Koo ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Screening Method ◽  
The Novel
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