Primary culture media for routine urine processing.

1982 ◽  
Vol 16 (4) ◽  
pp. 632-636 ◽  
Author(s):  
J C Fung ◽  
B Lucia ◽  
E Clark ◽  
M Berman ◽  
J Goldstein ◽  
...  
Keyword(s):  
Primary Culture ◽  
Culture Media ◽  
Urine Processing

Isolated osteoclasts in primary culture: first observations on structure and survival in culture media

1982 ◽  
Vol 165 (3) ◽  
pp. 405-413 ◽  
Author(s):  
Alberta Zambonin Zallone ◽  
Anna Teti ◽  
Maria Vittoria Primavera
Keyword(s):  
Primary Culture ◽  
Culture Media

scholarly journals Misinformation from sputum cultures without microscopic examination

1977 ◽  
Vol 6 (5) ◽  
pp. 518-527
Author(s):  
H S Heineman ◽  
J K Chawla ◽  
W M Lopton
Keyword(s):  
Primary Culture ◽  
Microscopic Examination ◽  
Culture Media ◽  
Respiratory Pathogens ◽  
Gram Stain ◽  
Cell Composition ◽  
Routine Manner ◽  
Hospital Study ◽  
Microscopic Findings

Only 13 of 38 hospital laboratories surveyed include a Gram stain routinely in microbiological sputum examination. In a prospective three-hospital study, 60% of over 1,200 "sputum" specimens consisted predominantly of saliva, as judged by cell composition. Compared with the results of cultures in which microorganisms presumptively identified on sputum smears were specifically sought ("directed cultures"), cultures of the same specimens processed in the routine manner missed pneumococci 61%, haemophili 23%, and yeasts 44% of the time. The findings were similar in all three hospitals despite differences in administration, staffing, primary culture media, and workload. Unless microscopic examination is routinely included, half of all microbiological information rendered on sputum specimens is meaningless and subject to dangerous misapplication. Furthermore, culture must be guided by microscopic findings, or respiratory pathogens will frequently be missed. Finally, when routine culture and smear disagree, the culture cannot be assumed to be correct. Microscopic examination should be mandatory in sputum microbiology, both for specimen evaluation and as a guide to what to look for in culture.

scholarly journals Evaluation of Chromogenic agar media for rapid identification of Candida species.

2017 ◽  
Vol 9 (1) ◽  
pp. 22-26
Author(s):  
Ismet Nigar ◽  
Shirin Tarafdar ◽  
Rehana Razzak Khan ◽  
S.M. Ali Ahmed ◽  
Ahmed Abu Saleh
Keyword(s):  
Primary Culture ◽  
Sensitivity And Specificity ◽  
Candida Species ◽  
Culture Media ◽  
Rapid Identification ◽  
Vaginal Swab ◽  
Clinical Specimens ◽  
Identification Of Species ◽  
Chromogenic Agar ◽  
Agar Media

Rapid identification of Candida isolates to the species level is essential in order to optimize the antifungal treatment. This study aimed to isolate and identify different species of Candida from various clinical specimens and to evaluate the use of chromogenic agar media as primary culture media for culture of Candida as well as for rapid identification of Candida species. A total of 100 different clinical specimens were studied (oral swab 35, high vaginal swab 28, catheterized urine 15, nail 14, bronchoalveolar lavage 04 and peritoneal fluid 04). Isolation of Candida species was done by primary culture in Sabouraud Dextrose Agar (SDA). Subsequent identification of species was performed by germ tube test, carbohydrate assimilation test (with commonly used twelve sugars) and subculture in chromogenic agar medium. Out of 64 isolated Candida, C. albicans 33 (51.53%) was the most predominant Candida species followed by C. tropicalis 17 (26.56%). The species of C. glabrata was 4 (6.25%), C. parapsilosis 4 (6.25%), C. krusei 3 (4.68%) and C.guilliermondii 2 (3.2%). One of the isolated Candida species was unidentified. The sensitivity and specificity of chromogenic agar media for C. albicans were as 96.97% and 96.87% respectively. The sensitivity and specificity for C. tropicalis were 94.12% and 97.87% respectively. C. krusei and C. glabrata both showed 100% sensitivity and specificity on chromogenic agar media. Efficacy of chromogenic agar media is nearly similar to carbohydrate assimilation method in species identification of Candida.Bangladesh J Med Microbiol 2015; 9 (1): 22-26

Microscopy and culture of fungal disease

2017 ◽  
Author(s):  
Gillian S. Shankland
Keyword(s):  
Fungal Infection ◽  
Primary Culture ◽  
Culture Media ◽  
Clinical Information ◽  
Fungal Disease ◽  
Rapid Diagnosis ◽  
Clinical Samples ◽  
Presumptive Diagnosis ◽  
Selection Of ◽  
Mycological Laboratory

The clinical mycological laboratory strives to provide an accurate and rapid diagnosis of suspected fungal disease, or exclude its possibility. The most common and most traditional methods for demonstrating fungi in tissue and body fluids are microscopy and culture. The techniques discussed in this chapter are not exhaustive but have proved to be efficient for the visualization and recovery of fungi from clinical samples. In general, these methods do not require expensive or specialist equipment. The clinician’s presumptive diagnosis may help with the selection of the most appropriate specimen and aid the laboratory in the method of processing and selection of the primary culture media. However, all too often there is scant clinical information provided to the laboratory, and indeed a fungal infection may initially not have been suspected.

scholarly journals Döderlein's Vaginal Bacillus: A Contribution to the Study of the Lacto-Bacilli

1931 ◽  
Vol 31 (3) ◽  
pp. 375-381 ◽  
Author(s):  
Robert Cruickshank
Keyword(s):  
Primary Culture ◽  
Culture Media ◽  
Morphological Type ◽  
Vaginal Flora ◽  
Blood Agar ◽  
Serological Relationship ◽  
Artificial Culture ◽  
Serological Type ◽  
Subsequent Propagation

It is concluded from a study of the morphological and cultural characters of 60 strains of Döderlein's vaginal bacillus that the organism belongs to the lacto-bacillus group of bacteria.Primary culture of Döderlein's bacillus may be readily obtained on lactose blood agar incubated aerobically provided the vaginal flora is of “Grade A” type, but its subsequent propagation on artificial culture media is less likely to be successful.Three morphological types of Döderlein's bacillus are recognised. Cultural and fermentative tests show that these types are all biologically similar.There is no serological homogeneity among the strains of Döderlein's bacillus, although there was some evidence of a serological relationship among the members of the commonest morphological type. The same serological type tends to persist in the vagina throughout pregnancy. Döderlein's bacillus and lacto-bacilli derived from other sources are not serologically identical.

High-pressure freezing of cells in suspension for low-voltage scanning electron microscopy (LVSEM)

1991 ◽  
Vol 49 ◽  
pp. 64-65
Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Low Voltage ◽  
Culture Media ◽  
Cell Structure ◽  
Freeze Substitution ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Cell Culture Media ◽  
Voltage Scanning

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.

Kalinin: A new epithelial basement membrane adhesion molecule

1991 ◽  
Vol 49 ◽  
pp. 178-179
Author(s):  
Douglas R. Keene ◽  
Gregory P. Lunstrum ◽  
Patricia Rousselle ◽  
Robert E. Burgeson
Keyword(s):  
Basement Membrane ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Human Keratinocytes ◽  
Positive Staining ◽  
Human Amnion ◽  
Epithelial Basement Membrane ◽  
Type Vii Collagen ◽  
Keratinocyte Cell ◽  
Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

Sign in / Sign up

Export Citation Format

Share Document