Disk diffusion interpretive criteria for extended-spectrum cephalosporins with Haemophilus influenzae.

1988 ◽  
Vol 26 (9) ◽  
pp. 1887-1889 ◽  
Author(s):  
J H Jorgensen ◽  
L A Maher ◽  
J S Redding
Keyword(s):  
Haemophilus Influenzae ◽  
Disk Diffusion ◽  
Extended Spectrum

scholarly journals Evaluation of extended spectrum beta lactamase enzymes prevalence in clinical isolates of Escherichia coli

2011 ◽  
Vol 2 (1) ◽  
pp. 8
Author(s):  
Ronak Bakhtiari ◽  
Jalil Fallah Mehrabadi ◽  
Hedroosha Molla Agamirzaei ◽  
Ailar Sabbaghi ◽  
Mohammad Mehdi Soltan Dallal
Keyword(s):  
Escherichia Coli ◽  
Disk Diffusion ◽  
Confirmatory Test ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Beta Lactamase ◽  
Disk Diffusion Method ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

Resistance to b-lactam antibiotics by gramnegative bacteria, especially <em>Escherichia coli (E. coli)</em>, is a major public health issue worldwide. The predominant resistance mechanism in gram negative bacteria particularly <em>E. coli </em>is via the production of extended spectrum beta lactamase (ESBLs) enzymes. In recent years, the prevalence of b-lactamase producing organisms is increased and identification of these isolates by using disk diffusion method and no-one else is not satisfactory. So, this investigation focused on evaluating the prevalence of ESBL enzymes by disk diffusion method and confirmatory test (Combined Disk). Five hundred clinical samples were collected and 200 <em>E. coli </em>isolates were detected by standard biochemical tests. To performing initial screening of ESBLs was used from Disk diffusion method on <em>E. coli </em>isolates. A confirmation test (Combined Disk method) was performed on isolates of resistant to cephalosporin's indicators. Up to 70% isolates exhibited the Multi Drug Resistance phenotype. In Disk diffusion method, 128(64%) <em>E. coli </em>isolates which resistant to ceftazidime and cefotaxime while in Combined Disk, among 128 screened isolates, 115 (89.8%) isolates were detected as ESBLs producers. This survey indicate beta lactamase enzymes are playing a significant role in antibiotic resistance and correct detection of them in phenotypic test by using disk diffusion and combined Disk is essential for accurate recognition of ESBLs.

scholarly journals In Vitro Activity of Essential Oils Against Planktonic and Biofilm Cells of Extended-Spectrum β-Lactamase (ESBL)/Carbapenamase-Producing Gram-Negative Bacteria Involved in Human Nosocomial Infections

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 272 ◽  
Author(s):  
Ramona Iseppi ◽  
Alessandro Di Cerbo ◽  
Piero Aloisi ◽  
Mattia Manelli ◽  
Veronica Pellesi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Essential Oils ◽  
Disk Diffusion ◽  
Mentha Piperita ◽  
Thymus Vulgaris ◽  
Extended Spectrum ◽  
Disk Diffusion Assay ◽  
Diffusion Assay

The aim of this study was to analyze the antibacterial activity of four essential oils (EOs), Melaleuca alternifolia, Eucalyptus globulus, Mentha piperita, and Thymus vulgaris, in preventing the development and spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae, metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa and carbapenemase (KPC)-producing Klebsiella pneumoniae. A total of 60 strains were obtained from the stock collection from the Microbiology Laboratory of Hesperia Hospital, Modena, Italy. Twenty ESBL-producing E. coli, 5 K. pneumoniae, 13 KPC-producing K. pneumoniae, and 20 MBL-producing P. aeruginosa were cultured and reconfirmed as ESBL and carbapenamase producers. Polymerase chain reaction was used for the detection of genes responsible for antibiotic resistance (ESBL and KPC/MBL). Antibacterial activity of the EOs was determined using the agar disk diffusion assay, and minimal inhibitory concentrations (MICs) were also evaluated. Lastly, adhesion capability and biofilm formation on polystyrene and glass surfaces were studied in 24 randomly selected strains. M. alternifolia and T. vulgaris EOs showed the best antibacterial activity against all tested strains and, as revealed by agar disk diffusion assay, M. alternifolia was the most effective, even at low concentrations. This effect was also confirmed by MICs, with values ranging from 0.5 to 16 µg/mL and from 1 to 16 µg/mL, for M. alternifolia and T. vulgaris EOs, respectively. The EOs’ antibacterial activity compared to antibiotics confirmed M. alternifolia EO as the best antibacterial agent. T. vulgaris EO also showed a good antibacterial activity with MICs lower than both reference antibiotics. Lastly, a significant anti-biofilm activity was observed for the two EOs (*P < 0.05 and **P < 0.01 for M. alternifolia and T. vulgaris EOs, respectively). A good antibacterial and anti-biofilm activity of M. alternifolia and T. vulgaris EOs against all selected strains was observed, thus demonstrating a future possible use of these EOs to treat infections caused by ESBL/carbapenemase-producing strains, even in association with antibiotics.

Shortening the incubation time for the combination disk diffusion extended-spectrum β-lactamase (ESBL) confirmation test: how far can we go?

2017 ◽  
Vol 50 (3) ◽  
pp. 473-476
Author(s):  
Wouter van den Bijllaardt ◽  
Patricia C.M. Voermans ◽  
Anton G. Buiting ◽  
Johan W. Mouton ◽  
Anouk E. Muller
Keyword(s):  
Incubation Time ◽  
Disk Diffusion ◽  
Extended Spectrum ◽  
Confirmation Test

Evaluation of disk diffusion methods to detect low-level β-lactamase-negative ampicillin-resistant Haemophilus influenzae

Apmis ◽  
2011 ◽  
Vol 119 (6) ◽  
pp. 385-392 ◽  
Author(s):  
NIELS NØRSKOV-LAURITSEN ◽  
WINNIE RIDDERBERG ◽  
LISE T. ERIKSTRUP ◽  
KURT FUURSTED
Keyword(s):  
Haemophilus Influenzae ◽  
Disk Diffusion ◽  
Low Level

scholarly journals Extended-Spectrum β-Lactamases in Ireland, Including a Novel Enzyme, TEM-102

2003 ◽  
Vol 47 (8) ◽  
pp. 2572-2578 ◽  
Author(s):  
Dearbháile Morris ◽  
Colette O'Hare ◽  
Maura Glennon ◽  
Majella Maher ◽  
Geraldine Corbett-Feeney ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Clinical Laboratory ◽  
Enterobacter Cloacae ◽  
Disk Diffusion ◽  
National Committee ◽  
Esbl Production ◽  
Esbl Gene ◽  
Extended Spectrum ◽  
The Family ◽  
Esbl Producers

ABSTRACT Organisms producing extended-spectrum β-lactamases (ESBLs) have been reported in many countries, but there is no information on the prevalence of ESBL-producing members of the family Enterobacteriaceae in Ireland. A total of 925 isolates of ampicillin-resistant members of the Enterobacteriaceae were received from six hospitals in Ireland over a 3-year period from September 1996 to September 1999. Isolates were screened for ESBL production by the double-disk diffusion (DDD) method. DDD-positive isolates that were (i) confirmed as ESBL producers by National Committee for Clinical Laboratory Standards (NCCLS) confirmatory testing and (ii) susceptible to cefoxitin by disk diffusion were considered ESBL producers. By these criteria, 27 (3%) of the ampicillin-resistant members of the Enterobacteriaceae studied were categorized as ESBL producers. Molecular typing suggested that some intra- and interhospital spread of ESBL-producing isolates had occurred. DNA sequencing of amplified bla TEM and bla SHV genes resulted in the detection of a novel bla TEM ESBL gene, bla TEM-102 in two isolates (Klebsiella pneumoniae and Enterobacter cloacae) received from the same hospital but isolated from different patients. The study suggests dissemination of ESBL-producing bacteria within the health care system in Ireland and emphasizes the need for measures to control such spread.

Development of interpretive criteria for tebipenem disk diffusion susceptibility testing with Staphylococcus spp. and Haemophilus influenzae

2011 ◽  
Vol 17 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Momoko Fujisaki ◽  
Shinya Sadamoto ◽  
Masanari Ikedo ◽  
Kyoichi Totsuka ◽  
Mitsuo Kaku ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Susceptibility Testing ◽  
Disk Diffusion ◽  
Staphylococcus Spp
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