scholarly journals Multiplex PCR assay specific for the multidrug-resistant strain W of Mycobacterium tuberculosis.

1994 ◽  
Vol 32 (6) ◽  
pp. 1542-1546 ◽  
Author(s):  
B B Plikaytis ◽  
J L Marden ◽  
J T Crawford ◽  
C L Woodley ◽  
W R Butler ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Resistant Strain ◽  
Multidrug Resistant ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Multidrug Resistant Strain

scholarly journals Molecular detection of antibiotic resistance genes in multidrug-resistant Staphylococcus aureus isolates from dog dental plaque

2019 ◽  
Vol 22 (4) ◽  
pp. 419-427
Author(s):  
S. Nouri Gharajalar ◽  
M. Onsori
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Multiplex Pcr ◽  
Resistance Genes ◽  
Dental Plaque ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Multidrug resistant Staphylococcus aureus strains are a major health care problem both in humans and animals. In this work we described three multiplex PCR assays for detection of clinically relevant antibiotic resistance genes in S. aureus isolated from dog dental plaques. Thirty dental plaque samples were collected; then cultural, biochemical and molecular tests performed for isolation and identification of S. aureus from samples. The antibiotic susceptibility of the isolates were checked by Kirby Bauer disc diffusion method and the prevalence of antibiotic resistance genes determined using multiplex PCR assay. As a result S. aureus was isolated from 18 dog plaque samples. Fifteen of these isolates were resistant to penicillin. The mecA gene was more prevalent than blaZ among penicillin-resistant bacteria. Ten of the isolates were resistant to tetracycline. The percentage of tetM was higher than tetK among them. Also, 10 of the isolates were resistant to cefazolin among them bla TEM detected in higher rate than blaSHV and blaOXA-1. Hence multiplex PCR assay is a suitable method for detection of antibiotic resistance patterns of S. aureus isolates.

Selection of Medicinal Plants Suppressing Growth of Multidrug-Resistant Strain of Tuberculosis Causative Agent

2021 ◽  
pp. 2115-2118
Author(s):  
Irina Alexandrovna Ratnikova ◽  
Amankeldi Kurbanovich Sadanov ◽  
Nina Nicolaevna Gavrilova ◽  
Saltanat Emilkyzy Orazymbet ◽  
Raushan Zhumabekkyzy Kaptagai
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Medicinal Plants ◽  
Aqueous Extract ◽  
Causative Agent ◽  
Resistant Strain ◽  
Multidrug Resistant ◽  
Alcohol Extract ◽  
Selection Of ◽  
Multidrug Resistant Strain

The article describes selection of medicinal plants active against multidrug-resistant strain of tuberculosis causative agent. It has been discovered that all tested extracts of medicinal plants in 1:20 dilutions were active regarding multidrug-resistant strain of Mycobacterium tuberculosis T-320 except for hackberry aqueous extract. The most active was alcohol extract of parmelia, which completely suppressed growth of mycobacteria in 1:100 dilution on the 21st day of cultivation.

scholarly journals Mycobacterium tuberculosis Multidrug Resistant Strain M Induces an Altered Activation of Cytotoxic CD8+ T Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e97837 ◽  
Author(s):  
Laura Geffner ◽  
Juan Ignacio Basile ◽  
Noemí Yokobori ◽  
Denise Kviatcovsky ◽  
Carmen Sabio y García ◽  
...  
Keyword(s):  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
Cd8 T Cells ◽  
Resistant Strain ◽  
Multidrug Resistant ◽  
Multidrug Resistant Strain

Multiplex PCR assay to aid in the indentification of the highly transmissible Mycobacterium tuberculosis strain CDC1551

1999 ◽  
Vol 79 (5) ◽  
pp. 273-278 ◽  
Author(s):  
B.B. Plikaytis ◽  
N. Kurepina ◽  
C.L. Woodley ◽  
R. Fleischmann ◽  
B. Kreiswirth ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Pcr Assay ◽  
Tuberculosis Strain ◽  
Multiplex Pcr Assay ◽  
Mycobacterium Tuberculosis Strain

scholarly journals Molecular Characterization of BacillusStrains Involved in Outbreaks of Anthrax in France in 1997

1998 ◽  
Vol 36 (11) ◽  
pp. 3412-3414 ◽  
Author(s):  
Guy Patra ◽  
Josée Vaissaire ◽  
Martine Weber-Levy ◽  
Claudine Le Doujet ◽  
Michèle Mock
Keyword(s):  
Multiplex Pcr ◽  
Tandem Repeat ◽  
Resistant Strain ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Soil Samples ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Biological Methods

Outbreaks of anthrax zoonose occurred in two regions of France in 1997. Ninety-four animals died, and there were three nonfatal cases in humans. The diagnosis of anthrax was rapidly confirmed by bacteriological and molecular biological methods. The strains ofBacillus anthracis in animal and soil samples were identified by a multiplex PCR assay. They all belonged to the variable-number tandem repeat (VNTR) group (VNTR)3. A penicillin-resistant strain was detected. Nonvirulent bacilli related to B. anthracis, of all VNTR types, were also found in the soil.

Mixed-strain infection with a drug-sensitive and multidrug-resistant strain of Mycobacterium tuberculosis

The Lancet ◽  
1995 ◽  
Vol 345 (8963) ◽  
pp. 1512-1513 ◽  
Author(s):  
A. Theisen ◽  
C. Reichel ◽  
S. Rüsch-Gerdes ◽  
W.H. Haas ◽  
J.K. Rockstroh ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Resistant Strain ◽  
Multidrug Resistant ◽  
Multidrug Resistant Strain
Sign in / Sign up

Export Citation Format

Share Document