Comparison of the Quantitative Formalin Ethyl Acetate Concentration Technique and Agar Plate Culture for Diagnosis of Human Strongyloidiasis

Abstract Background This study reports the prevalence of Ov/minute intestinal fluke (MIF) and Taenia infections among inhabitants of the Kenethao district, northern Lao PDR. Methods Fecal samples from 580 inhabitants were examined using the formalin-ethyl acetate concentration technique. Results The prevalence of Ov/MIF, Taenia spp. and coinfection was 45.3, 11.9 and 6.1%, respectively. There was no significant difference between males and females for Ov/MIF (p=0.813) and Taenia infection (p=0.759). The prevalence of Ov/MIF was significantly associated with age (p=0.005), but not for Taenia infection (p=0.836). Consumption of raw fish (p=0.001) and raw meat (p=0.046) was significantly associated with parasitic infections. Conclusions The results suggest that Ov/MIF and Taenia spp. are highly endemic in this area and there is a need for projects to eliminate these parasites.

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Research on the Catalytic Activity of MnyCezOx/TiO2 for Ethyl Acetate Oxidation

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.308 ◽

2013 ◽

Vol 781-784 ◽

pp. 308-311 ◽

Cited By ~ 1

Author(s):

Xin Li ◽

Wei Su ◽

Qi Bin Xia ◽

Zhi Meng Liu

Keyword(s):

Catalytic Activity ◽

Ethyl Acetate ◽

High Activity ◽

Fixed Bed ◽

Space Velocity ◽

Fixed Bed Reactor ◽

Impregnation Method ◽

Acetate Oxidation ◽

Acetate Concentration ◽

Molar Ratios

Manganese and cerium based catalysts with different Mn/Ce molar ratios prepared by impregnation method for ethyl acetate oxidation. The activity tests of the samples were performed in a fixed-bed reactor. The effect of gas hourly space velocity (GHSV) and ethyl acetate concentration on the catalytic activity of the catalyst were also investigated. The results showed that these catalysts had high activity for the catalytic oxidation of ethyl acetate, of which the catalyst Mn0.9Ce0.1Ox/TiO2exhibitedthe bestactivity, and the temperature required for 90% conversion of ethyl acetate was at 216 °C. The catalyst Mn0.9Ce0.1Ox/TiO2still maintained high activity in the range of GHSV (16,500 to 48,500 h-1) and ethyl acetate concentration (4526 to 7092 mg/m3). In additional, experiments for measuring stability of Mn0.9Ce0.1Ox/TiO2were carried out, and experimental results showed that the good stability of Mn0.9Ce0.1Ox/TiO2was kept after it has run for 25 hours.

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Low Sensitivity of the Formol-Ethyl Acetate Sedimentation Concentration Technique in Low-Intensity Schistosoma japonicum Infections

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0000386 ◽

2009 ◽

Vol 3 (2) ◽

pp. e386 ◽

Cited By ~ 12

Author(s):

Tore Lier ◽

Gunnar S. Simonsen ◽

Tianping Wang ◽

Dabing Lu ◽

Hanne H. Haukland ◽

...

Keyword(s):

Ethyl Acetate ◽

Schistosoma Japonicum ◽

Low Intensity ◽

Concentration Technique ◽

Low Sensitivity

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Chromatographic behaviour of some porphyrins and their complexes with Zn, Pd(II) and Cu(II)

Journal of Porphyrins and Phthalocyanines ◽

10.1002/(sici)1099-1409(200003)4:2<209::aid-jpp197>3.0.co;2-f ◽

2000 ◽

Vol 04 (02) ◽

pp. 209-214 ◽

Cited By ~ 3

Author(s):

M. I. UVAROVA ◽

G. D. BRYKINA ◽

O. A. SHPIGUN

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Ethyl Acetate ◽

Mobile Phase ◽

High Performance ◽

Reversed Phase ◽

Zinc Complexes ◽

Acetate Concentration ◽

Hydrophobic Compounds ◽

Retention Parameters

In this work the influence of the porphyrin structure and of the nature of the mobile phase upon retention parameters is examined by means of reversed phase high-performance liquid chromatography (HPLC). Acetonitrile–ethyl acetate and other mixtures were used as eluents. An increase in the retention of azo- and benzosubstituted porphyrins as well as of those containing a large number of carbon atoms as substituents of macrocycles may be noted. A variation in the polarity of the mobile phase affects the retention of the ligands more than that of their zinc complexes. The retention of the most hydrophobic compounds may be well described in coordinates lg k'– lg M. For less hydrophobic porphyrins these dependences are close to linear only within limited intervals of mobile phase ethyl acetate concentration. The best separation of zinc complexes was achieved with acetonitrile as the eluent. The detection limit of porphyrin ligands and complexes with metals is n × 10-8 M.

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Evaluation OfFormol-ethyl acetate Concentration Method In The Diagnosis Of IntestinalParasitic Infections

Sohag Medical Journal ◽

10.21608/smj.2019.40607 ◽

2019 ◽

Vol 23 (1) ◽

pp. 1-5

Author(s):

Nada El-Nadi ◽

Amany A. Abd el laah ◽

Amal Ahmed ◽

Noha Ahmed

Keyword(s):

Ethyl Acetate ◽

Acetate Concentration ◽

Concentration Method

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Formalin-ethyl acetate concentration, FLOTAC Pellet and anal swab techniques for the diagnosis of intestinal parasites

Parasitology Research ◽

10.1007/s00436-018-6054-9 ◽

2018 ◽

Vol 117 (11) ◽

pp. 3567-3573 ◽

Cited By ~ 7

Author(s):

Paola Cociancic ◽

Laura Rinaldi ◽

María Lorena Zonta ◽

Graciela Teresa Navone

Keyword(s):

Ethyl Acetate ◽

Intestinal Parasites ◽

Acetate Concentration ◽

Anal Swab

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Agar Plate Culture: An Alternative Sensitive Routine Laboratory Detection Method for Strongyloides stercoralis and Hookworm Parasites

Iranian Journal of Parasitology ◽

10.18502/ijpa.v16i1.5514 ◽

2021 ◽

Author(s):

Tadesse HAILU ◽

Endalkachew NIBRET ◽

Arancha AMOR ◽

Abaineh MUNSHEA ◽

Melaku ANEGAGRIE

Keyword(s):

Detection Rate ◽

Agar Plate ◽

Strongyloides Stercoralis ◽

Human Infection ◽

Diagnostic Methods ◽

Diagnostic Agreement ◽

Chi Square ◽

Concentration Technique ◽

Kappa Value ◽

Stool Samples

Background: Human infection with Strongyloides stercoralis and hookworm parasites is usually under reported due to less sensitive diagnostic methods. Agar plate culture (APC) is the most sensitive technique for parasites having larval stage. However, using APC in routine diagnosis is uncommon. This study aimed to determine the detection rate and sensitivity of APC in comparison with formal ether concentration technique (FECT) and spontaneous tube sedimentation techniques (STSTs) for S. stercoralis and hookworm larvae. Methods: Stool samples collected from 844 schoolchildren in Amhara Regional State, northwestern Ethiopia in 2019, transported to nearby health institutions and processed by APC, FECT and STSTs. The prevalence of S. stercoralis and hookworm was computed by descriptive statistics and Chi-square. The diagnostic agreement among the three techniques was evaluated using Kappa value. Results: The overall prevalence of S. stercoralis and hookworm infections by combining the three methods was 13.2% (111/844) and 33.8% (277/844), respectively. Using APC alone, the prevalence of S. stercoralis and hookworm were found to be 10.9% (92/844) and 24.5% (207/844), respectively. Agar plate culture was 5.4 and 2.7 times respectively more sensitive than FECT and STST, with slight and fair agreement in the detection of S. stercoralis. Hookworm diagnostic agreement was moderate between APC and FECT, and APC and STST. The Kappa value between STST and FECT diagnostic methods was substantial. Conclusion: APC has a better detection rate of S stercoralis and hookworm larvae. Therefore, APC can be used as an alternative routine diagnostic method to S. stercoralis and hookworm co-endemic countries.

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