scholarly journals Rapid slide coagglutination test for identifying and typing group B streptococci

1977 ◽  
Vol 6 (3) ◽  
pp. 266-270
Author(s):  
M K Kirkegaard ◽  
C R Field
Keyword(s):  
Staphylococcus Aureus ◽  
Blind Study ◽  
Blood Agar ◽  
Acid Extract ◽  
Group B Streptococci ◽  
Type Group ◽  
Precipitin Test ◽  
Group B

A Cowan I strain of Staphylococcus aureus was labeled with either group B streptococcal grouping or typing antiserum. These antibody-labeled reagent cells (ARC) were used in a slide coagglutination test to identify and type group B streptococci from blood agar plates. All streptococci were also identified by the standard Lancefield capillary precipitin test. In a blind study, all 141 group B streptococci were correctly identified by the coagglutination grouping test. None of the 148 non-group B streptococci caused agglutination of ARC. The coagglutination grouping test required an acid extract prepared from only four colonies and could be completed less than 30 min after colonies were removed from plates. The coagglutination typing test correctly identified 98.6% of the types of the 141 group B streptococcal strains tested. At least 88.6% of these streptococci could be typed directly from blood agar plates within 5 min by the coagglutination typing test. The remaining 11.4% of the group B streptococci were acid extracted (less than a 30-min procedure), and the extract was used for coagglutination typing. Coagglutination typing can be performed with only four colonies. The coagglutination grouping and typing tests are inexpensive, rapid, reliable, and easy to perform.

scholarly journals Identification of Staphylococcus aureus and Group B Streptococci from Bovine Mastitis Cases by Latex Agglutination

1995 ◽  
Vol 48 (1) ◽  
pp. 13-17
Author(s):  
Ichiro NAKAYA ◽  
Etsuji HAKOGI ◽  
Yoshiyuki MARUO ◽  
Yuji OGURA ◽  
Susumu NOMA ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Mastitis ◽  
Latex Agglutination ◽  
Group B Streptococci ◽  
Group B

scholarly journals Synergistic hemolysis phenomenon shown by an alpha-toxin-producing Clostridium perfingens and streptococcal CAMP factor in presumptive streptococcal grouping

1978 ◽  
Vol 8 (5) ◽  
pp. 480-488
Author(s):  
S M Gubash
Keyword(s):  
Human Blood ◽  
Test Group ◽  
Blood Agar ◽  
Group A Streptococci ◽  
Alpha Toxin ◽  
Group B Streptococci ◽  
Camp Factor ◽  
Group A ◽  
Camp Test ◽  
Group B

A new phenomenon of synergistic hemolysis by Clostridium perfringens alpha-toxin and the streptococcal CAMP factor on human and guinea pig erythrocytes is described. A possible mode of action of the CAMP factors is suggested. On human blood agar all of the tested isolates of group B streptococci gave an arrowhead-shaped zone of hemolysis; 74% of group A gave a crescent-shaped lytic zone, whereas all isolates of groups C and G and the remaining 26% of group A streptococci gave a bullet-shaped lytic zone. By comparison, in the CAMP test incubated aerobically and anaerobically, 70 and 91%, respectively, of streptococci other than group B gave positive, arrowhead-shaped lytic zones. If all intermediate positive reactions in the CAMP tests were read as negative after aerobic incubation, only 89% of group B streptococci would be properly identified. The synergistic hemolysis phenomenon, using an alpha-toxin-producing C. perfringens and human blood agar, provided a reliable test for presumptive identification of group B streptococci, with promising potential to differentiate in the same test group A streptococci from other groups.

CAMP test v1

2021 ◽  
Author(s):  
lydiariver not provided
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Blood Agar ◽  
Camp Factor ◽  
Camp Test ◽  
Group B

Group B Streptococcus agalactiae has CAMP factor which allow it to hemolized zones when it is grown on blood agar plates near to Staphylococcus aureus ATCC 25293 colonies, this effect is brought about by Staphylococcus aureus sphingomyelinase.

Cross protection in mice with the Smith diffuse strain of Staphylococcus aureus versus a type Ia strain of group B streptococci

1982 ◽  
Vol 28 (7) ◽  
pp. 726-732 ◽  
Author(s):  
Yoshitoshi Ichiman ◽  
Kosaku Yoshida
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Surface ◽  
Active Immunization ◽  
Cell Vaccine ◽  
Group B Streptococci ◽  
Type Ia ◽  
Whole Cell Vaccine ◽  
Surface Polysaccharides ◽  
Surface Polysaccharide ◽  
Group B

Active immunization of mice with whole cell vaccine or cell surface polysaccharide from either the Smith diffuse strain of Staphylococcus aureus or SS-615 (type Ia of group B streptococci) protected against challenge by either the homologous or heterologous strains. In the peritoneal cavity of mice immunized with either of these organisms rapid phagocytosis and reduction of the viable cells was observed at 6 h after the challenge. Cell surface polysaccharides extracted from strains Smith diffuse and SS-615, both prepared by the same procedure as that of the Smith surface antigen, were capable of absorbing the protective antibody in rabbit hyperimmune sera prepared with homologous or heterologous strains.

scholarly journals Analysis of the microbial flora in breast abscess: a retrospective cohort study conducted in the emergency department

2017 ◽  
Vol 4 (7) ◽  
pp. 2143 ◽  
Author(s):  
Ramya Ramakrishnan ◽  
Ramakrishnan V. Trichur ◽  
Sowmya Murugesan ◽  
Srihari Cattamanchi
Keyword(s):  
Staphylococcus Aureus ◽  
Cohort Study ◽  
Retrospective Cohort Study ◽  
Retrospective Cohort ◽  
Microbial Flora ◽  
Breast Abscess ◽  
Group B Streptococci ◽  
Culture Sensitivity ◽  
Group B ◽  
Age Range

Background: Breast abscess develops as a complication of lactational mastitis. Recently, there is an increase in the incidence of non-lactational breast abscess. The aim of the study was to analyse the microbial flora in the lactational and non-lactational breast abscess in the ED and to elucidate the susceptibility of flora to different antibiotics.Methods: This is a retrospective cohort study of breast abscess patients registered in the ED over two years. The case records of these patients were retrieved, and the details of their age, clinical presentation, investigation, and treatment were recorded. Specifically, the pus culture sensitivity and antibiotics used in the management of these patients were noted, and the pattern of microbiological flora analyzed.Results: A total of 124 patients were included in the study, with 97 women were categorized into lactational breast abscess, and 27 women were classified as a non-lactational breast abscess. Mean age was 24 years of age (Range 18 to 56 years). The majority of the women were young between 18 and 34 years of age (83%). The culture was grown in 92% of the patient pus specimens. Staphylococcus aureus (83%) was common organisms cultured in lactational breast abscess. Mixed Flora including Staphylococcus aureus and Group B Streptococci were grown in non-lactational breast abscess.Conclusions: Appropriate antibiotic choices are of immense importance in the management of breast abscess. Mixed flora is common in non - lactational breast abscess when compared with a lactational breast abscess. Staphylococcus Aureus is the most common isolate in both groups.

scholarly journals Rapid isolation and identification of group B streptococci from selective broth medium by slide co-agglutination test

1978 ◽  
Vol 8 (4) ◽  
pp. 410-412
Author(s):  
G Szilagyi ◽  
E Mayer ◽  
A I Eidelman
Keyword(s):  
Early Detection ◽  
False Positive ◽  
Agglutination Test ◽  
Clinical Isolates ◽  
Blood Agar ◽  
Group B Streptococci ◽  
Isolation And Identification ◽  
Direct Identification ◽  
Rapid Isolation ◽  
Group B

Direct identification of group B streptococci from a selective broth medium was performed with the Phadebact streptococcus test to determine the feasibility of this technique for early detection of streptococcal colonization. Of 124 clinical isolates, 122 (98.4%) were correctly identified in less than 24 h from the selective broth medium, whereas standard cultures from blood agar plates identified, after 48 h, only 66 (53.2%). The presence of group B streptococci in mixed cultures was always detected by the Phadebact test, and no false-positive co-agglutination tests were observed in 372 cultures from which organisms other than group B streptococci were isolated.

scholarly journals Genital Flora during Pregnancy and Colonization of the Newborn

1980 ◽  
Vol 73 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Jean M Ross ◽  
J R Needham
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pregnant Women ◽  
Isolation Rate ◽  
Group B Streptococci ◽  
Vaginal Microflora ◽  
Postnatal Mothers ◽  
Similar Range ◽  
Group B ◽  
And Staphylococcus Aureus

A longitudinal study of the cervical and vaginal microflora of 131 pregnant women showed a similar range of organisms at first visit (up to 16 weeks), at 28 weeks and at 36 weeks of pregnancy. Twenty different groups or genera of microorganisms were recovered, predominantly lactobacilli. There was a fall in the isolation rate of organisms in the mothers 6 to 8 weeks after delivery. Lactobacilli and yeasts including Candida albicans were recovered less frequently whereas the incidence of Escherichia coli and group B streptococci increased. Infants bom to these mothers were swabbed within 24 hours of delivery and yielded organisms from the umbilicus, ear and mouth in 24%, 33% and 38% of cases respectively. Alpha haemolytic streptococci and Staphylococcus aureus were the predominant organisms. Sixteen different groups or genera were isolated. The isolation rates in non-pregnant women attending a ‘well-women’ clinic were similar to those in the postnatal mothers; few women of 60 years or more were colonized.

A novel pcr format for screening of methicillin-resistant Staphylococcus aureus and group b streptococci in swabs

Pathology ◽  
2019 ◽  
Vol 51 ◽  
pp. S138-S139
Author(s):  
Christopher J. McIver ◽  
Ranapanada Nilushika D. De Silva ◽  
Noel Er ◽  
Ryanbi Pratama ◽  
Chinmoy Mukerjee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Group B Streptococci ◽  
Methicillin Resistant ◽  
Group B
Sign in / Sign up

Export Citation Format

Share Document