Cross protection in mice with the Smith diffuse strain of Staphylococcus aureus versus a type Ia strain of group B streptococci

1982 ◽  
Vol 28 (7) ◽  
pp. 726-732 ◽  
Author(s):  
Yoshitoshi Ichiman ◽  
Kosaku Yoshida
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Surface ◽  
Active Immunization ◽  
Cell Vaccine ◽  
Group B Streptococci ◽  
Type Ia ◽  
Whole Cell Vaccine ◽  
Surface Polysaccharides ◽  
Surface Polysaccharide ◽  
Group B

Active immunization of mice with whole cell vaccine or cell surface polysaccharide from either the Smith diffuse strain of Staphylococcus aureus or SS-615 (type Ia of group B streptococci) protected against challenge by either the homologous or heterologous strains. In the peritoneal cavity of mice immunized with either of these organisms rapid phagocytosis and reduction of the viable cells was observed at 6 h after the challenge. Cell surface polysaccharides extracted from strains Smith diffuse and SS-615, both prepared by the same procedure as that of the Smith surface antigen, were capable of absorbing the protective antibody in rabbit hyperimmune sera prepared with homologous or heterologous strains.

scholarly journals Identification of Staphylococcus aureus and Group B Streptococci from Bovine Mastitis Cases by Latex Agglutination

1995 ◽  
Vol 48 (1) ◽  
pp. 13-17
Author(s):  
Ichiro NAKAYA ◽  
Etsuji HAKOGI ◽  
Yoshiyuki MARUO ◽  
Yuji OGURA ◽  
Susumu NOMA ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Mastitis ◽  
Latex Agglutination ◽  
Group B Streptococci ◽  
Group B

scholarly journals Isolation, chemical composition, and molecular size of extracellular type II and type Ia polysaccharides of group B streptococci.

1983 ◽  
Vol 41 (2) ◽  
pp. 527-534 ◽  
Author(s):  
B J De Cueninck ◽  
T F Greber ◽  
T K Eisenstein ◽  
R M Swenson ◽  
G D Shockman
Keyword(s):  
Chemical Composition ◽  
Molecular Size ◽  
Type Ii ◽  
Group B Streptococci ◽  
Type Ia ◽  
Group B

scholarly journals Heterogeneity of penicillin-non-susceptible group B streptococci isolated from a single patient in Germany

2019 ◽  
Vol 75 (2) ◽  
pp. 296-299 ◽  
Author(s):  
Mark van der Linden ◽  
Rafael Mamede ◽  
Natascha Levina ◽  
Peter Helwig ◽  
Pedro Vila-Cerqueira ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Genomic Analysis ◽  
Amino Acid Sequences ◽  
Capsular Type ◽  
Group B Streptococci ◽  
The Us ◽  
Type Ia ◽  
The Usa ◽  
Group B ◽  
Host Evolution

Abstract Objectives Streptococcus agalactiae [group B streptococci (GBS)] have been considered uniformly susceptible to penicillin. However, increasing reports from Asia and North America are documenting penicillin-non-susceptible GBS (PRGBS) with mutations in pbp genes. Here we report, to the best of our knowledge, the first two PRGBS isolates recovered in Europe (AC-13238-1 and AC-13238-2), isolated from the same patient. Methods Two different colony morphologies of GBS were noted from a surgical abscess drainage sample. Both were serotyped and antimicrobial susceptibility testing was performed by different methodologies. High-throughput sequencing was done to compare the isolates at the genomic level, to identify their capsular type and ST, to evaluate mutations in the pbp genes and to compare the isolates with the genomes of other PRGBS isolates sharing the same serotype and ST. Results Isolates AC-13238-1 and AC-13238-2 presented MICs above the EUCAST and CLSI breakpoints for penicillin susceptibility. Both shared the capsular type Ia operon and ST23. Genomic analysis uncovered differences between the two isolates in seven genes, including altered pbp genes. Deduced amino acid sequences revealed critical substitutions in PBP2X in both isolates. Comparison with serotype Ia clonal complex 23 PRGBS from the USA reinforced the similarity between AC-13238-1 and AC-13238-2, and their divergence from the US strains. Conclusions Our results support the in-host evolution of β-lactam-resistant GBS, with two PRGBS variants being isolated from one patient.

Binding of staphylococcal cell surface polysaccharide to human fibrinogen

1990 ◽  
Vol 36 (3) ◽  
pp. 206-210
Author(s):  
Toshichika Ohtomo ◽  
Tsugiaki Kobayashi ◽  
Yukio Ohshima ◽  
Yukio Usui ◽  
Masaru Suganuma ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Surface ◽  
Binding Site ◽  
Key Words ◽  
Active Substance ◽  
Soft Agar ◽  
Alkaline Ph ◽  
Human Fibrinogen ◽  
Surface Polysaccharide ◽  
Alkaline Range

The interaction between the binding site of a polysaccharide (called compact colony forming active substance (CCFAS)), obtained from the cell surface of a strain of Staphylococcus, and human fibrinogen (HF) was investigated. The CCFAS was found to bind specifically to both the Bβ and γ chains of HF at pH 7.0 and 8.0, and the Aα chain at pH 5.0. The binding of CCFAS with fibrinogen fragments obtained by digestion with plasmin were also investigated. Fragments with Mr of 55 000, 24 000, and 19 000 were the major bands precipitated by CCFAS at pH 7.0 and 8.0. Fragments with Mr of 85 000 and 75 000 bound to CCFAS at pH 5.0. Binding of CCFAS (7 μg) with fibrinogen could be inhibited by 1.2 μg of Bβ chain and 1.5 μg γ chain at alkaline pH or 6.2 μg of the Aα chain at pH 5.0. CCFAS was, therefore, assumed to be specifically bonded with HF molecules, in the alkaline range at least, resulting in compact colony forming activity in serum soft agar and paracoagulation. Key words: cell surface, polysaccharide, Staphylococcus aureus, fibrinogen.

scholarly journals Sialic acid content and surface hydrophobicity of group B streptococci

1993 ◽  
Vol 110 (1) ◽  
pp. 87-94 ◽  
Author(s):  
L. A. Teixeira ◽  
A. M. S. Figueiredo ◽  
B. T. Ferreira ◽  
V. M. L. Alves ◽  
P. E. Nagao ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Acid Content ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Gas Liquid Chromatography ◽  
Group B Streptococci ◽  
Sialic Acid Content ◽  
Asymptomatic Patients ◽  
Group B

SUMMARYThe sialic acid content and the cell-surface hydrophobicity index of 40 group B streptococci (GBS) strains were assessed. GBS isolated from invasive infections (virulent strains) presented an increased level of sialic acid content (1.4%) when compared with GBS isolated from asymptomatic patients (0.53%). Treatment of GBS strain 85634 with neuraminidase resulted in a decrease (about 25%) in the net negative surface charge as assessed by cell electrophoresis. This finding suggests that sialic acid residues are important anionogenic groups exposed on GBS cell surface.N-acetylneuraminic acid was the only sialic acid derivative characterized in the strain 85634 as evaluated by gas-liquid chromatography. GBS from different serotypes presented a hydrophobic index mean value of 0.9. Even though the sialic acid contributed effectively to the negative charge on GBS cell surface, no difference was observed in the hydrophobic index when virulent and avirulent strains were compared.

scholarly journals Disruption of the Putative Cell Surface Polysaccharide Biosynthesis Gene SO3177 in Shewanella oneidensis MR-1 Enhances Adhesion to Electrodes and Current Generation in Microbial Fuel Cells

2010 ◽  
Vol 76 (13) ◽  
pp. 4151-4157 ◽  
Author(s):  
Atsushi Kouzuma ◽  
Xian-Ying Meng ◽  
Nobutada Kimura ◽  
Kazuhito Hashimoto ◽  
Kazuya Watanabe
Keyword(s):  
Cell Surface ◽  
Shewanella Oneidensis ◽  
Insertion Mutant ◽  
Wild Type ◽  
Current Generation ◽  
Polysaccharide Biosynthesis ◽  
Transposon Insertion ◽  
Biosynthesis Gene ◽  
Surface Polysaccharides ◽  
Surface Polysaccharide

ABSTRACT A microbial fuel cell (MFC) was inoculated with a random transposon insertion mutant library of Shewanella oneidensis MR-1 and operated with lactate as the sole fuel to select for mutants that preferentially grew in it. Agar plate cultivation of the resultant MFC enrichment culture detected an increased number of colonies exhibiting rough morphology. One such isolate, strain 4A, generated 50% more current in an MFC than wild-type MR-1. Determination of the transposon insertion site in strain 4A followed by deletion and complementation experiments revealed that the SO3177 gene, encoding a putative formyltransferase and situated in a cell surface polysaccharide biosynthesis gene cluster, was responsible for the increased current. Transmission electron microscopy showed that a layered structure at the cell surface, stainable with ruthenium red, was impaired in the SO3177 mutant (ΔSO3177), confirming that SO3177 is involved in the biosynthesis of cell surface polysaccharides. Compared to the wild type, ΔSO3177 cells preferentially attached to graphite felt anodes in MFCs, while physicochemical analyses revealed that the cell surface of ΔSO3177 was more hydrophobic. These results demonstrate that cell surface polysaccharides affect not only the cell adhesion to graphite anodes but also the current generation in MFCs.

Antibody-mediated protection against Staphylococcus aureus dermonecrosis and sepsis by a whole cell vaccine

Vaccine ◽  
2017 ◽  
Vol 35 (31) ◽  
pp. 3834-3843 ◽  
Author(s):  
Fan Zhang ◽  
Maria Jun ◽  
Olivia Ledue ◽  
Muriel Herd ◽  
Richard Malley ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Vaccine ◽  
Whole Cell ◽  
Whole Cell Vaccine

scholarly journals Analysis of the microbial flora in breast abscess: a retrospective cohort study conducted in the emergency department

2017 ◽  
Vol 4 (7) ◽  
pp. 2143 ◽  
Author(s):  
Ramya Ramakrishnan ◽  
Ramakrishnan V. Trichur ◽  
Sowmya Murugesan ◽  
Srihari Cattamanchi
Keyword(s):  
Staphylococcus Aureus ◽  
Cohort Study ◽  
Retrospective Cohort Study ◽  
Retrospective Cohort ◽  
Microbial Flora ◽  
Breast Abscess ◽  
Group B Streptococci ◽  
Culture Sensitivity ◽  
Group B ◽  
Age Range

Background: Breast abscess develops as a complication of lactational mastitis. Recently, there is an increase in the incidence of non-lactational breast abscess. The aim of the study was to analyse the microbial flora in the lactational and non-lactational breast abscess in the ED and to elucidate the susceptibility of flora to different antibiotics.Methods: This is a retrospective cohort study of breast abscess patients registered in the ED over two years. The case records of these patients were retrieved, and the details of their age, clinical presentation, investigation, and treatment were recorded. Specifically, the pus culture sensitivity and antibiotics used in the management of these patients were noted, and the pattern of microbiological flora analyzed.Results: A total of 124 patients were included in the study, with 97 women were categorized into lactational breast abscess, and 27 women were classified as a non-lactational breast abscess. Mean age was 24 years of age (Range 18 to 56 years). The majority of the women were young between 18 and 34 years of age (83%). The culture was grown in 92% of the patient pus specimens. Staphylococcus aureus (83%) was common organisms cultured in lactational breast abscess. Mixed Flora including Staphylococcus aureus and Group B Streptococci were grown in non-lactational breast abscess.Conclusions: Appropriate antibiotic choices are of immense importance in the management of breast abscess. Mixed flora is common in non - lactational breast abscess when compared with a lactational breast abscess. Staphylococcus Aureus is the most common isolate in both groups.

scholarly journals Lethal synergism induced in mice by influenza type A virus and type Ia group B streptococci.

1983 ◽  
Vol 41 (2) ◽  
pp. 618-623 ◽  
Author(s):  
W T Jones ◽  
J H Menna ◽  
D E Wennerstrom
Keyword(s):  
Type A ◽  
Group B Streptococci ◽  
Influenza Type ◽  
Type Ia ◽  
Group B

scholarly journals TWO SEROLOGICAL TYPES OF GROUP B HEMOLYTIC STREPTOCOCCI WITH RELATED, BUT NOT IDENTICAL, TYPE-SPECIFIC SUBSTANCES

1938 ◽  
Vol 67 (1) ◽  
pp. 25-40 ◽  
Author(s):  
Rebecca C. Lancefield
Keyword(s):  
Group B Streptococci ◽  
Passive Protection ◽  
Cross Reactions ◽  
Precipitin Reaction ◽  
Specific Substance ◽  
Type Ia ◽  
Preliminary Study ◽  
The Cross ◽  
Group B ◽  
Identical Type

1. Among group B hemolytic streptococci one serological type previously described as homogeneous has been shown, instead, to contain two closely related types, distinguishable by reciprocal absorption experiments. These streptococci are designated Types Ia and Ib. 2. Homologous organisms in each case absorb all antibodies from their respective antisera, while organisms of the heterologous related type absorb only the antibody responsible for the cross reactions. Group B streptococci of other types do not absorb the antibodies responsible for the cross reactions between these two related types. The precipitin reaction and passive protection tests in mice were employed in this analysis. 3. The type-specific substance of Type Ia is a polysaccharide. Preliminary study indicates that this is also true of Type Ib. While no data are available concerning the chemical relationships of these substances, it seems probable that the two types elaborate polysaccharides, related chemically as well as serologically.

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