scholarly journals Evaluation of Twenty-Three Blood Culture Media

1978 ◽  
Vol 8 (3) ◽  
pp. 288-292
Author(s):  
Jegdish P. Babu ◽  
Ronald F. Schell ◽  
Jack L. Le Frock
Keyword(s):  
Quality Control ◽  
Blood Culture ◽  
Recovery Rate ◽  
Culture Medium ◽  
Culture Media ◽  
Control Measures ◽  
Rate Of Recovery ◽  
The Difference

Several investigators have evaluated clinically a variety of commercially available blood culture media. No agreement has been reached as to which of these media is optimal for detection of bacteremia. The purpose of this study was to determine the rate of recovery of microorganisms from various blood culture media. A total of 23 blood culture media were inoculated with 7 to 15 microorganisms per bottle in the presence or absence of an erythrocyte-serum mixture. The results demonstrated that blood culture media differed in their ability to support the growth of microorganisms. At 4 days after inoculation, only 10 of the 23 blood culture media supported the growth of 91% (10 of the 11) or more of the test microorganisms. The recovery rate of microorganisms depended not only upon the type of medium but also upon the manufacturer of the type of blood culture medium. The addition of an erythrocyte-serum mixture to the blood culture media did not influence the difference in the recovery rate of microorganisms among media and the same type of medium prepared by different manufacturers. The majority (15 of the 23) of the blood culture media supplemented with the erythrocyte-serum mixture failed to support the growth of 91% or more of the test microorganisms at 4 days after inoculation. These results have demonstrated that blood culture media need to be improved. Better quality control measures should also be implemented to evaluate commercial blood culture media.

scholarly journals Evaluation of Analytical Performance of Variant II Turbo HbA1c Analyzer According to Sigma Metrics

2019 ◽  
Vol 38 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Giray Bozkaya ◽  
Nuriye Uzuncan ◽  
Sibel Bilgili ◽  
Ozlem Demirezen
Keyword(s):  
Quality Control ◽  
Control Measures ◽  
Analytical Performance ◽  
Major Constituent ◽  
General Evaluation ◽  
Quality Control Materials ◽  
Allowable Error ◽  
The Difference ◽  
Analytical Variation

Summary Background: Hemoglobin A1c, (HbA1c) which is the major constituent of glycated hemoglobin, has been used in the follow-up of retrospective glycemia for years and in the diagnosis of diabetes mellitus nowadays. Since the analytical performance of HbA1c should be high likewise all laboratory tests, various quality control measures are used. Sigma metrics is one of these measures and it is the combination of bias, precision and total allowable error that ensures a general evaluation of analytical quality. The aim of our study was to evaluate the analytical performance of Bio-Rad’s Variant Turbo II HbA1c analyzer according to sigma metrics. Methods: Sigma levels were calculated using the data obtained from two levels of internal and 12 external quality control materials (Bio-Rad) of Variant II Turbo HbA1c analyzer according to s= (TEa% - Bias%) / CV% formula. Results: The mean sigma levels for low and high quality control materials were found to be 3.0 and 4.1, respectively. Conclusions: The annual mean analytical performance of Variant II Turbo HbA1c analyzer was found to be acceptable according to sigma metrics. In order to be sure of the difference in HbA1c results indicating the success or failure in treatment but not arise from analytical variation, it is thought that more stringent quality control measures should be applied to reach higher sigma levels.

Recovery of Haemophilus influenzae from twenty-three blood culture media

1979 ◽  
Vol 9 (1) ◽  
pp. 84-87
Author(s):  
R F Schell ◽  
J L Le Frock ◽  
J P Babu ◽  
D B Robinson
Keyword(s):  
Blood Culture ◽  
Haemophilus Influenzae ◽  
Culture Medium ◽  
Culture Media ◽  
Optimal Recovery ◽  
Becton Dickinson ◽  
Recovery Rates ◽  
Growth Requirements

Haemophilus influenzae is an important agent of bacteremia and has fastidious growth requirements. The purpose of this investigation was to determine the ability of commercial blood culture media to support the growth of this fastidious microorganism. Twenty-three types of blood culture media were inoculated with individual suspensions of eight strains of H. influenzae in the presence or absence of an erythrocyte-serum mixture. The rates of recovery of the H. influenzae strains from the various types of blood culture media were compared. The results demonstrated that the type of medium, the manufacturer, the erythrocyte-serum mixture, and the strain of H. influenzae influenced the recovery rates of H. influenzae. Optimal recovery of the strains of H. influenzae was obtained from brain heart infustion blood culture medium (GIBCO). Trypic soy broth (GIBCO) and supplemental peptone of Becton, Dickinson and Co. also were found to be superior to the remaining types of media tested for the recovery of H. influenzae.

Improved blood culture medium for radiometric detection of yeasts

1979 ◽  
Vol 9 (3) ◽  
pp. 448-449
Author(s):  
R L Hopfer ◽  
K Mills ◽  
D Gröschel
Keyword(s):  
Blood Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Medium Composition ◽  
Co2 Production ◽  
Yeast Nitrogen Base ◽  
Nitrogen Base ◽  
System A ◽  
Radiometric Detection ◽  
Triton X

The influence of medium composition on the rate of CO2 production by yeasts in simulated blood cultures was determined in the Bactec radiometric system. A yeast nitrogen base broth containing [14C]glucose and Triton X-100 allowed the detection of positive cultures earlier than the regular Bactec blood culture media.

Turbidimetric Method: A New, Ecological, and Fast Way to Evaluate of Vancomycin Potency

2020 ◽  
Vol 103 (6) ◽  
pp. 1582-1587
Author(s):  
Patrícia Aleixa do Nascimento ◽  
Ana Carolina Kogawa ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Analytical Chemistry ◽  
Quality Control ◽  
Culture Medium ◽  
Culture Media ◽  
Pharmaceutical Product ◽  
Green Analytical Chemistry ◽  
Microbiological Method ◽  
Turbidimetric Method ◽  
Microbiological Methods ◽  
Routine Quality Control

Abstract Background Vancomycin, an antimicrobial, has many microbiological methods in literature, but it was not found any that follows the green chemistry principles. Objective The aim of this work was to develop and validate a new microbiological analytical method with a green view to determine the vancomycin potency in lyophilized powder using less quantity of diluents and culture medium, minimizing the costs and reducing the time of analysis. Methods The objective will be achieved using the microbiological method by turbidimetry. Results Water was used as the diluent to prepare the vancomycin solution. BHI broth as used as culture media for the growth of the S. aureus ATCC 25923. The method was linear in the range of 30, 39 and 50.7 µg/mL. It was selective, with vancomycin reference and sample absorbance values very similar. The precision of the method was proved at intraday (RSD 4.42 %), interday (RSD 3.56 %) and intermediate levels (RSD 2.03%). It was accurate with mean recovery of 100.71 % and robust when changes were performed in three parameters of the method and analyzed by the F-Test and t-Test. Conclusions The method for evaluating the potency of vancomycin in pharmaceutical product was successfully developed and validated. Highlights The method can be applied to routine quality control of vancomycin product as an alternative that contemplates the green analytical chemistry and the current pharmaceutical analyzes.

scholarly journals Potential Of Microalgae Chlorella vulgaris As Bioremediation Agents of Heavy Metal Pb (Lead) On Culture Media

2018 ◽  
Vol 31 ◽  
pp. 05010 ◽  
Author(s):  
Endah Rita Sulistya Dewi ◽  
Riza Nuravivah
Keyword(s):  
Heavy Metal ◽  
Experimental Study ◽  
Chlorella Vulgaris ◽  
Metal Content ◽  
Culture Medium ◽  
Culture Media ◽  
Average Growth ◽  
Aas Method ◽  
The Difference ◽  
Pb Concentration

The purpose of this study to determine the ability of Chlorella vulgaris in absorbing Pb (lead) and the effect of the variation of Pb metal concentration on the growth of Chlorella vulgaris.This study using an experimental study with complete random design with 4 treatments, namely control (without the addition of metal), Pb1 (addition of metal 1 mg / l), Pb3 (3 mg / l) and Pb5 (5 mg / l), respectively 3 replications. Exposure Pb ion in Chlorella vulgaris for 7 days. Analysis of the metal content of Pb concentration performed on culture media after exposure it at 3 hours after dispersion Chlorella vulgaris and on day 7 of culture using the AAS method. Do also counting the growth of cells each day. The results of the analysis of the average metal content of Pb in the culture medium at the end of the study was the control (0.1980), Pb1 (0.1453), Pb3 (0.4144) and Pb5 (0.5305). While the average growth of Chlorella vulgaris at the end of the study were control (630.1116 x 104), Pb1 (829.0012 x 104), Pb3 (1069.9446 x 104) and Pb 5 (808.94450 x 104). The results of the analysis of the content of Pb in the F test shown that the difference in concentration of water Pb given real influence on the ability of Chlorella vulgaris in absorbing Pb and growth. The conclusion of this study was Chlorella vulgaris has the ability to absorb metals in the waters, and the provision of various concentrations of Pb can affect the growth of Chlorella vulgaris.

scholarly journals Effectiveness of Resins in Neutralizing Antibiotic Activities in Bactec Plus Aerobic/F Culture Medium

1998 ◽  
Vol 36 (12) ◽  
pp. 3731-3733 ◽  
Author(s):  
J. Spaargaren ◽  
C. P. A. van Boven ◽  
G. P. Voorn
Keyword(s):  
Blood Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Detection Time

Incorporating resins in blood culture media can effectively reduce the activities of several antibiotics. It was shown that the activities of some generally used antibiotics decreased by 80 to 90% within 2 h in Bactec Plus Aerobic/F resin-containing culture medium. Bactec vials containing resins were still found to be positive for bacteria when antibiotics were present. The addition of β-lactamase shortened the detection time irrespective of the presence of resins.

scholarly journals Ways to Improve Quality Control of Culture Medium Used for Mycoplasma Detection

2019 ◽  
Vol 19 (3) ◽  
pp. 161-168
Author(s):  
S. M. Sukhanova ◽  
Z. E. Berdnikova ◽  
A. S. Tikhonova
Keyword(s):  
Quality Control ◽  
Russian Federation ◽  
Culture Medium ◽  
Culture Media ◽  
Growth Promotion ◽  
The State ◽  
Test Strain ◽  
Animal Blood ◽  
Mycoplasma Detection ◽  
The Russian Federation

An urgent safety concern associated with biological products is contamination with mycoplasmas, which may originate from donor tissues and organs, virus harvests, culture medium components, trypsin, animal blood serum, as well as be transmitted by personnel involved in the manufacture of medicines. Currently, due to an increase in the range of biologicals available, there is a need for more sensitive and specific test methods. In the Russian practice, microbiological (culture-based) testing of finished pharmaceutical products for mycoplasma contamination is performed using complex culture media whose sensitivity depends on the quality of proteins, ingredients, and reagents used. Growth promotion properties of the media are determined according to the State Pharmacopoeia of the Russian Federation, 14th ed., using a single test strain — Mycoplasma arginini G230 (M. arginini G230 industry reference material). The aim of the study was to analyse current Russian and foreign requirements for the quality control of culture media that are used for mycoplasma detection, in order to update and improve the quality control procedure in Russia. It was demonstrated that a compelling advantage of the State Pharmacopoeia of the Russian Federation is the possibility of using a semi-liquid culture medium which does not require special aerobic or anaerobic incubation conditions and allows for quantification of mycoplasma colonies and determination of mycoplasma titre in culture medium while testing its growth promotion properties using reference М. arginini G230 test strain. The analysis revealed some differences in Russian and foreign requirements for quality evaluation of culture media. These differences were taken into account when developing recommendations for improvement of the Russian test procedure, i.e. enlarging the range of test strains used and development of respective reference standards.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals Untargeted Metabolomics Approach for the Discovery of Environment-Related Pyran-2-ones Chemodiversity in a Marine-Sourced Penicillium restrictum

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 378
Author(s):  
Van-Tuyen Le ◽  
Samuel Bertrand ◽  
Thibaut Robiou du Pont ◽  
Fabrice Fleury ◽  
Nathalie Caroff ◽  
...  
Keyword(s):  
Culture Medium ◽  
Blue Mussel ◽  
Culture Media ◽  
Principal Component ◽  
Chemical Diversity ◽  
Untargeted Metabolomics ◽  
High Chemical ◽  
Medium Mass ◽  
Metabolomics Study ◽  
Penicillium Restrictum

Very little is known about chemical interactions between fungi and their mollusc host within marine environments. Here, we investigated the metabolome of a Penicillium restrictum MMS417 strain isolated from the blue mussel Mytilus edulis collected on the Loire estuary, France. Following the OSMAC approach with the use of 14 culture media, the effect of salinity and of a mussel-derived medium on the metabolic expression were analysed using HPLC-UV/DAD-HRMS/MS. An untargeted metabolomics study was performed using principal component analysis (PCA), orthogonal projection to latent structure discriminant analysis (O-PLSDA) and molecular networking (MN). It highlighted some compounds belonging to sterols, macrolides and pyran-2-ones, which were specifically induced in marine conditions. In particular, a high chemical diversity of pyran-2-ones was found to be related to the presence of mussel extract in the culture medium. Mass spectrometry (MS)- and UV-guided purification resulted in the isolation of five new natural fungal pyran-2-one derivatives—5,6-dihydro-6S-hydroxymethyl-4-methoxy-2H-pyran-2-one (1), (6S, 1’R, 2’S)-LL-P880β (3), 5,6-dihydro-4-methoxy-6S-(1’S, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (4), 4-methoxy-6-(1’R, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (6) and 4-methoxy-2H-pyran-2-one (7)—together with the known (6S, 1’S, 2’S)-LL-P880β (2), (1’R, 2’S)-LL-P880γ (5), 5,6-dihydro-4-methoxy-2H-pyran-2-one (8), (6S, 1’S, 2’R)-LL-P880β (9), (6S, 1’S)-pestalotin (10), 1’R-dehydropestalotin (11) and 6-pentyl-4-methoxy-2H-pyran-2-one (12) from the mussel-derived culture medium extract. The structures of 1-12 were determined by 1D- and 2D-MMR experiments as well as high-resolution tandem MS, ECD and DP4 calculations. Some of these compounds were evaluated for their cytotoxic, antibacterial, antileishmanial and in-silico PTP1B inhibitory activities. These results illustrate the utility in using host-derived media for the discovery of new natural products.

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