scholarly journals Establishment and Characterization of an Air-Liquid Canine Corneal Organ Culture Model To Study Acute Herpes Keratitis

2014 ◽  
Vol 88 (23) ◽  
pp. 13669-13677 ◽  
Author(s):  
R. M. Harman ◽  
L. Bussche ◽  
E. C. Ledbetter ◽  
G. R. Van de Walle
Keyword(s):  
Organ Culture ◽  
Culture Model ◽  
Herpes Keratitis ◽  
Corneal Organ Culture

scholarly journals An Assay System to Evaluate Riboflavin/UV-A Corneal Phototherapy Efficacy in a Porcine Corneal Organ Culture Model

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 730
Author(s):  
Anna Perazzi ◽  
Chiara Gomiero ◽  
Livio Corain ◽  
Ilaria Iacopetti ◽  
Enrico Grisan ◽  
...  
Keyword(s):  
Organ Culture ◽  
Diagnostic Methods ◽  
Assay System ◽  
Automated Image Analysis ◽  
Ocular Structure ◽  
Culture Model ◽  
Significant Difference ◽  
Corneal Organ Culture ◽  
Structure Investigations

The purpose of this study was to investigate the response of porcine corneal organ cultures to riboflavin/UV-A phototherapy in the injury healing of induced lesions. A porcine corneal organ culture model was established. Corneal alterations in the stroma were evaluated using an assay system, based on an automated image analysis method able to (i) localize the holes and gaps within the stroma and (ii) measure the brightness values in these patches. The analysis has been performed by dividing the corneal section in 24 regions of interest (ROIs) and integrating the data analysis with a “multi-aspect approach.” Three group of corneas were analyzed: healthy, injured, and injured-and-treated. Our study revealed a significant effect of the riboflavin/UV-A phototherapy in the injury healing of porcine corneas after induced lesions. The injured corneas had significant differences of brightness values in comparison to treated (p < 0.00) and healthy (p < 0.001) corneas, whereas the treated and healthy corneas showed no significant difference (p = 0.995). Riboflavin/UV-A phototherapy shows a significant effect in restoring the brightness values of damaged corneas to the values of healthy corneas, suggesting treatment restores the injury healing of corneas after lesions. Our assay system may be compared to clinical diagnostic methods, such as optical coherence tomography (OCT) imaging, for in vivo damaged ocular structure investigations.

Corneal Organ Culture Model for Assessing Epithelial Recovery After Surfactant Exposure

2004 ◽  
Vol 23 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Ke‐Ping Xu ◽  
Jing Zhang ◽  
Fu‐Shin X. Yu
Keyword(s):  
Organ Culture ◽  
Culture Model ◽  
Corneal Organ Culture

scholarly journals Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

10.3791/58562 ◽  
2019 ◽  
Author(s):  
Nileyma Castro ◽  
Stephanie R. Gillespie ◽  
Audrey M. Bernstein
Keyword(s):  
Wound Healing ◽  
Organ Culture ◽  
Ex Vivo ◽  
Culture Model ◽  
Corneal Organ Culture

scholarly journals Vascular Endothelial Growth Factor Blockade Induces Dermal Endothelial Cell Apoptosis in a Clinically Relevant Skin Organ Culture Model

2020 ◽  
Vol 33 (3) ◽  
pp. 110-118
Author(s):  
Andrea Luengas-Martinez ◽  
Jonathan Hardman-Smart ◽  
David Rutkowski ◽  
Talveen S. Purba ◽  
Ralf Paus ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Organ Culture ◽  
Cell Apoptosis ◽  
Vascular Endothelial ◽  
Endothelial Cell Apoptosis ◽  
Culture Model ◽  
Endothelial Growth Factor ◽  
Skin Organ Culture

Experimental Study of the Survival of Metastatic Cancer Cells in Corneal Organ Culture

2006 ◽  
Vol 47 (4) ◽  
pp. 1339 ◽  
Author(s):  
Nilanjana Deb-Joardar ◽  
Gilles Thuret ◽  
Jean-Marc Dumollard ◽  
Lena Absi ◽  
Lydia Campos-Guyotat ◽  
...  
Keyword(s):  
Experimental Study ◽  
Organ Culture ◽  
Cancer Cells ◽  
Metastatic Cancer ◽  
Corneal Organ Culture ◽  
Metastatic Cancer Cells

scholarly journals The influence of donor factors on corneal organ culture contamination

2017 ◽  
Vol 95 (7) ◽  
pp. 733-740 ◽  
Author(s):  
Anja K. Gruenert ◽  
Katja Rosenbaum ◽  
Gerd Geerling ◽  
Thomas A. Fuchsluger
Keyword(s):  
Organ Culture ◽  
Donor Factors ◽  
Corneal Organ Culture

scholarly journals Analysis of the RNA content of the exosomes derived from blood serum and urine and its potential as biomarkers

2014 ◽  
Vol 369 (1652) ◽  
pp. 20130502 ◽  
Author(s):  
Mu Li ◽  
Emily Zeringer ◽  
Timothy Barta ◽  
Jeoffrey Schageman ◽  
Angie Cheng ◽  
...  
Keyword(s):  
Blood Serum ◽  
Cell Communication ◽  
Cell Culture Model ◽  
Culture Model ◽  
Wide Range ◽  
Abnormal Cells ◽  
A Cell ◽  
Invasive Diagnostics ◽  
Serum And Urine

Exosomes are tiny vesicles (30–150 nm) constantly secreted by all healthy and abnormal cells, and found in abundance in all body fluids. These vesicles, loaded with unique RNA and protein cargo, have a wide range of biological functions, including cell-to-cell communication and signalling. As such, exosomes hold tremendous potential as biomarkers and could lead to the development of minimally invasive diagnostics and next generation therapies within the next few years. Here, we describe the strategies for isolation of exosomes from human blood serum and urine, characterization of their RNA cargo by sequencing, and present the initial data on exosome labelling and uptake tracing in a cell culture model. The value of exosomes for clinical applications is discussed with an emphasis on their potential for diagnosing and treating neurodegenerative diseases and brain cancer.

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