scholarly journals The Corepressor CTBP2 Is a Coactivator of Retinoic Acid Receptor/Retinoid X Receptor in Retinoic Acid Signaling

2013 ◽  
Vol 33 (16) ◽  
pp. 3343-3353 ◽  
Author(s):  
Prashanth Kumar Bajpe ◽  
Guus J. J. E. Heynen ◽  
Lorenza Mittempergher ◽  
Wipawadee Grernrum ◽  
Iris A. de Rink ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Rna Interference ◽  
Nuclear Receptor ◽  
Transcriptional Control ◽  
Target Genes ◽  
Retinoic Acid Receptor ◽  
Retinoid X Receptor ◽  
Gene Promoters ◽  
Acid Receptor ◽  
Receptor Complexes

Retinoids play key roles in development, differentiation, and homeostasis through regulation of specific target genes by the retinoic acid receptor/retinoid X receptor (RAR/RXR) nuclear receptor complex. Corepressors and coactivators contribute to its transcriptional control by creating the appropriate chromatin environment, but the precise composition of these nuclear receptor complexes remains to be elucidated. Using an RNA interference-based genetic screen in mouse F9 cells, we identified the transcriptional corepressor CTBP2 (C-terminal binding protein 2) as a coactivator critically required for retinoic acid (RA)-induced transcription.CTBP2suppression by RNA interference confers resistance to RA-induced differentiation in diverse murine and human cells. Mechanistically, we find that CTBP2 associates with RAR/RXR at RA target gene promoters and is essential for their transactivation in response to RA. We show that CTBP2 is indispensable to create a chromatin environment conducive for RAR/RXR-mediated transcription by recruiting the histone acetyltransferase p300. Our data reveal an unexpected function of the corepressor CTBP2 as a coactivator for RAR/RXR in RA signaling.

Ligand-inducible retinoid X receptor-mediated protein: DNA interactions in the retinoic acid receptor β2 gene promoter in vivo

1998 ◽  
Vol 136 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Masato Ikeda ◽  
Remco A Spanjaard ◽  
Elizabeth W Noordhoek ◽  
Akio Kawaguchi ◽  
Toshimasa Onaya ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Retinoic Acid Receptor ◽  
Gene Promoter ◽  
Retinoid X Receptor ◽  
Dna Interactions ◽  
Acid Receptor ◽  
Protein Dna Interactions ◽  
Retinoic Acid Receptor Β2

scholarly journals Cooperative Therapeutic Action of Retinoic Acid Receptor and Retinoid X Receptor Agonists in a Mouse Model of Alzheimer's Disease

2014 ◽  
Vol 42 (2) ◽  
pp. 587-605 ◽  
Author(s):  
Kohichi Kawahara ◽  
Michita Suenobu ◽  
Hideyuki Ohtsuka ◽  
Akihiko Kuniyasu ◽  
Yukihiko Sugimoto ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Retinoic Acid ◽  
Mouse Model ◽  
Retinoic Acid Receptor ◽  
Retinoid X Receptor ◽  
Therapeutic Action ◽  
Acid Receptor ◽  
Receptor Agonists ◽  
Model Of Alzheimer’S Disease

scholarly journals Retinoic acid receptor α and retinoid-X receptor-specific agonists synergistically target telomerase expression and induce tumor cell death

Oncogene ◽  
2003 ◽  
Vol 22 (57) ◽  
pp. 9142-9150 ◽  
Author(s):  
Frédéric Pendino ◽  
Charles Dudognon ◽  
Francois Delhommeau ◽  
Tewfik Sahraoui ◽  
Maria Flexor ◽  
...  
Keyword(s):  
Cell Death ◽  
Retinoic Acid ◽  
Tumor Cell ◽  
Retinoic Acid Receptor ◽  
Retinoid X Receptor ◽  
Tumor Cell Death ◽  
Acid Receptor ◽  
Retinoic Acid Receptor Α

scholarly journals Activation of the phosphoenolpyruvate carboxykinase gene retinoic acid response element is dependent on a retinoic acid receptor/coregulator complex.

1992 ◽  
Vol 12 (12) ◽  
pp. 5527-5535 ◽  
Author(s):  
R K Hall ◽  
D K Scott ◽  
E L Noisin ◽  
P C Lucas ◽  
D K Granner
Keyword(s):  
Retinoic Acid ◽  
Transcriptional Control ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Mutational Analysis ◽  
Retinoic Acid Receptor ◽  
Retinoid Receptor ◽  
Response Element ◽  
Acid Receptor ◽  
Receptor Alpha ◽  
Retinoic Acid Response Element

The accessory factor 1 (AF1) element is an upstream transcriptional control region that plays a role in the response of the phosphoenolpyruvate carboxykinase (PEPCK) gene to both glucocorticoids and retinoic acid. We demonstrate here that retinoic acid receptor alpha (RAR alpha) binds to a sequence within the AF1 element, TGACCT (site B), that is a consensus retinoic acid response element (RARE) half-site. A similar DNA sequence, TGGCCG (site C), located 1 bp downstream of site B, is not involved in the binding of RAR alpha monomers or dimers but is required for the constitution of a functional RARE. Site C is also required for the formation of a complex involving RAR alpha and a liver nuclear factor designated CR, for coregulator. Mutational analysis of the AF1 element shows that the RAR alpha/CR complex is the trans-acting unit that mediates the retinoic acid response of the PEPCK gene. Another member of the retinoid receptor family, retinoid X receptor alpha (RXR alpha), can also form a complex with RAR alpha and the AF1 element. Several observations, including the observation that RXR alpha antibody interacts with CR, indicate that RXR alpha and CR are identical or closely related proteins. Through RXR alpha forms a complex with RAR alpha and the AF1 element, we demonstrate that the AF1 element is functionally distinguishable from a retinoid X response element. Taken together, our results show that the AF1 element contains an RARE that mediates a retinoic acid response by binding an RAR alpha/coregulator complex; this coregulator is presumably RXR alpha.

Expression of nuclear retinoic acid receptor and retinoid X receptor mRNA in the cornea and conjunctiva

1998 ◽  
Vol 17 (5) ◽  
pp. 462-469 ◽  
Author(s):  
Nicole M. Bossenbroek ◽  
Timothy H. Sulahian ◽  
John L. Ubels
Keyword(s):  
Retinoic Acid ◽  
Retinoic Acid Receptor ◽  
Retinoid X Receptor ◽  
Acid Receptor ◽  
Receptor Mrna

scholarly journals Existence of Retinoic Acid-Receptor-Independent Retinoid X-Receptor-Dependent Pathway in Myeloid Cell Function

2001 ◽  
Vol 85 (1) ◽  
pp. 60-69 ◽  
Author(s):  
Takayuki Hida ◽  
Kenji Tai ◽  
Naoki Tokuhara ◽  
Akira Ishibashi ◽  
Kouichi Kikuchi ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Cell Function ◽  
Retinoic Acid Receptor ◽  
Myeloid Cell ◽  
Retinoid X Receptor ◽  
Acid Receptor ◽  
Dependent Pathway
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