scholarly journals Full Genome Sequence of pT3, a Multiresistant Plasmid Carrying the mcr-3.5 Colistin Resistance Gene, Recovered from an Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolate from Crickets Sold as Food

2019 ◽  
Vol 8 (29) ◽  
Author(s):  
Katrin Zurfluh ◽  
Marc J. A. Stevens ◽  
Melissa Bucher ◽  
Laurent Poirel ◽  
Patrice Nordmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Resistance Gene ◽  
Genome Sequence ◽  
Resistance Genes ◽  
Full Genome Sequence ◽  
Full Genome ◽  
Colistin Resistance ◽  
Coli Isolate ◽  
Single Plasmid

Here, we sequenced multidrug resistance plasmid pT3, which encodes mcr-3.5 and other resistance genes. The cooccurrence of mcr genes and other resistance genes on a single plasmid is of concern.

scholarly journals Genome Sequence of Avian Escherichia coli Strain IHIT25637, an Extraintestinal Pathogenic E. coli Strain of ST131 Encoding Colistin Resistance Determinant MCR-1

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Christa Ewers ◽  
Stephan Göttig ◽  
Maria Bülte ◽  
Sophie Fiedler ◽  
Manuela Tietgen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Resistance Gene ◽  
Genome Sequence ◽  
Coli Strain ◽  
Sequence Type ◽  
Resistance Determinant ◽  
Colistin Resistance ◽  
E Coli ◽  
Expec Strain

Sequence type 131 (ST131) is one of the predominant Escherichia coli lineages among extraintestinal pathogenic E. coli (ExPEC) that causes a variety of diseases in humans and animals and frequently shows multidrug resistance. Here, we report the first genome sequence of an ST131-ExPEC strain from poultry carrying the plasmid-encoded colistin resistance gene mcr-1 .

First report of the plasmid-mediated colistin resistance gene mcr-1 in a clinical Escherichia coli isolate in Algeria

2016 ◽  
Vol 48 (6) ◽  
pp. 760-761 ◽  
Author(s):  
Betitera Yanat ◽  
Jesús Machuca ◽  
Radia Dali Yahia ◽  
Abdelaziz Touati ◽  
Álvaro Pascual ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Colistin Resistance ◽  
Coli Isolate ◽  
First Report

scholarly journals Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Bao-Tao Liu ◽  
Feng-Jing Song ◽  
Ming Zou ◽  
Zhi-Hui Hao ◽  
Hu Shan
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Cronobacter Sakazakii ◽  
Colistin Resistance ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant

ABSTRACT We report the presence of mcr-1 in Escherichia coli and carbapenem-resistant Cronobacter sakazakii from the same diseased chicken. The mcr-1 gene linked with ISApl1 was located on two different IncI2 plasmids, including one multidrug plasmid in E. coli, whereas fosA3-bla NDM-9 was on an IncB/O plasmid in C. sakazakii. The development of the fosA3-bla NDM-9 resistance region was mediated by IS26. The colocation of mcr-1 or bla NDM-9 with other resistance genes will accelerate the dissemination of the two genes.

scholarly journals Autochthonous case of mobile colistin resistance gene mcr-1 from a uropathogenic Escherichia coli isolate in Sétif Hospital, Algeria

2019 ◽  
Vol 19 ◽  
pp. 356-357 ◽  
Author(s):  
Larbi Zakaria Nabti ◽  
Farida Sahli ◽  
Linda Hadjadj ◽  
Edgarthe Priscilla Ngaiganam ◽  
David Lupande-Mwenebitu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Uropathogenic Escherichia Coli ◽  
Colistin Resistance ◽  
Coli Isolate ◽  
Autochthonous Case

scholarly journals Complete Genome Sequence of a Colistin Resistance Gene ( mcr-1 )-Bearing Isolate of Escherichia coli from the United States

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Richard J. Meinersmann ◽  
Scott R. Ladely ◽  
James L. Bono ◽  
Jodie R. Plumblee ◽  
M. Carolina Hall ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Resistance Gene ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
The United States ◽  
Colistin Resistance ◽  
E Coli

Transmissible colistin resistance conferred by the mcr-1 gene-bearing IncI2 plasmid has been recently reported in Escherichia coli in the United States. We report here the completed genome sequence of a second E. coli strain isolated from swine in the United States that carried the mcr-1 gene on an IncI2-type plasmid.

scholarly journals ISEc69-Mediated Mobilization of the Colistin Resistance Gene mcr-2 in Escherichia coli

2021 ◽  
Vol 11 ◽  
Author(s):  
Yu-Zhang He ◽  
Teng-Fei Long ◽  
Bing He ◽  
Xing-Ping Li ◽  
Gong Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Sequencing ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Insertion Sequence ◽  
Molecular Mechanisms ◽  
Clinical Treatment ◽  
Host Genome ◽  
Conjugative Plasmid ◽  
Colistin Resistance

ObjectivesThe emergence of mobile colistin resistance genes has compromised the efficacy of the last resort antibiotic, colistin, in clinical treatment. The mcr-2 gene was first identified in Belgium in association with the insertion sequence ISEc69. However, the molecular mechanisms of mcr-2 mobilization are not well understood.MethodsTo further explore the mobilization of mcr-2 gene via ISEc69, we constructed a conjugative plasmid that carries an intact composite transposon Tn7052. Transposition assays were performed by conjugation, the transposition sites were characterized by arbitrary primed PCR and DNA sequencing.ResultsIn this study, we experimentally demonstrated that mcr-2 could be mobilized as a composite transposon Tn7052 and its transposition generated 8-bp AT-rich duplications in the host genome.ConclusionThese results indicate that mcr-2 gene could be mobilized by ISEc69, the current investigations provide mechanistic insights in the transposition of mcr-2.

scholarly journals IncX2 and IncX1-X2 Hybrid Plasmids Coexisting in a FosA6-Producing Escherichia coli Strain

2017 ◽  
Vol 61 (7) ◽  
Author(s):  
Qinglan Guo ◽  
Jiachun Su ◽  
Christi Lee McElheny ◽  
Nicole Stoesser ◽  
Yohei Doi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Resistance Genes ◽  
Coli Strain ◽  
Colistin Resistance ◽  
Content Type ◽  
Accessory Genes

ABSTRACT IncX plasmids are receiving much attention as vehicles of carbapenem and colistin resistance genes, such as bla NDM, bla KPC, and mcr-1. Among them, IncX2 subgroup plasmids remain rare. Here, we characterized IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain that were possibly generated as a consequence of recombination events between an R6K-like IncX2 plasmid and a pLN126_33-like IncX1 plasmid. Variable multidrug resistance mosaic regions were observed in these plasmids, indicating their potential to serve as flexible carriers of resistance genes. The diversity of IncX group plasmid backbones and accessory genes and the evolution of hybrid IncX plasmids pose a challenge in detecting and classifying them.

scholarly journals First IncHI2 Plasmid Carrying mcr-9.1 , bla VIM-1 , and Double Copies of bla KPC-3 in a Multidrug-Resistant Escherichia coli Human Isolate

mSphere ◽  
2021 ◽  
Author(s):  
Serena Simoni ◽  
Marina Mingoia ◽  
Andrea Brenciani ◽  
Maria Carelli ◽  
Maria M. Lleò ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Multidrug Resistant ◽  
Human Isolate ◽  
Colistin Resistance ◽  
Class 1 Integron ◽  
Content Type ◽  
Single Plasmid ◽  
Class 1

We report a novel IncHI2 plasmid coharboring bla VIM-1 , two copies of bla KPC-3 and mcr-9.1 resistance genes in a human Escherichia coli of the new serogroup O188. The bla VIM-1 gene was included in a class 1 integron, mcr-9.1 in a cassette bracketed by IS 903 and ΔIS1R, and bla KPC-3 in two copies within a new composite Tn 4401 -like transposon. The emergence of carbapenem and colistin resistance genes in a single plasmid is of great concern for upcoming clinical therapies.

scholarly journals Co-existence of plasmid-mediated tigecycline and colistin resistance genes tet(X4) and mcr-1 in a community-acquired Escherichia coli isolate in China

2020 ◽  
Vol 75 (11) ◽  
pp. 3400-3402
Author(s):  
Zhi Ruan ◽  
Huiqiong Jia ◽  
Hangfei Chen ◽  
Jianyong Wu ◽  
Fang He ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Colistin Resistance ◽  
Coli Isolate
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