ABSTRACT
The Study for Monitoring Antimicrobial Resistance Trends (SMART) global surveillance program collected 103,960 isolates of
Enterobacteriaceae
from 2008 to 2014. From this isolate collection, all ertapenem-nonsusceptible isolates (MIC, ≥1 μg/ml;
n
= 3,428) and 9,371 isolates of
Escherichia coli
,
Klebsiella pneumoniae
,
Klebsiella oxytoca
, and
Proteus mirabilis
with an ertapenem-susceptible extended-spectrum-β-lactamase (ESBL)-positive phenotype were assessed for the presence of common carbapenemase genes using a Check-MDR CT101 microarray (Check-Points, Wageningen, the Netherlands) and published multiplex PCR assays. Testing identified 1,493 isolates that harbored a carbapenemase gene (1,485 ertapenem-nonsusceptible isolates and 8 ertapenem-susceptible ESBL-positive isolates) and accounted for 1.4% (1,493/103,960) of all isolates of
Enterobacteriaceae
. The most frequently identified carbapenemase genes were the KPC (
n
= 794), OXA-48-like (
n
= 300), and NDM (
n
= 290) genes. Carbapenemase genes were most frequently identified in
Klebsiella pneumoniae
(
n
= 1,127),
Escherichia coli
(
n
= 149), and
Enterobacter cloacae
(
n
= 110). Among the carbapenemase-positive isolates, 66.7% (2/3), 37.0% (111/300), 20.0% (8/40), 3.3% (3/92), 2.3% (18/794), and 0% (0/290) of the isolates with genes for GES, OXA-48-like, IMP, VIM, KPC, and NDM, respectively, were susceptible to imipenem (MIC, ≤1 μg/ml). Isolates that tested as susceptible to imipenem were not uncommon among carbapenemase-positive isolates (9.4%, 141/1,493) and most frequently carried OXA-48-like enzymes (78.7%; 111/141); however, overall, these isolates remained rare (0.1%, 141/103,960). The practice of screening clinical isolates of
Enterobacteriaceae
that test as susceptible to carbapenems
in vitro
for the presence of carbapenemase genes remains controversial and requires further study.