scholarly journals Subgingival Microbiome Colonization and Cytokine Production during Early Dental Implant Healing

mSphere ◽  
2017 ◽  
Vol 2 (6) ◽  
Author(s):  
Jeffrey B. Payne ◽  
Paul G. Johnson ◽  
Car Reen Kok ◽  
João C. Gomes-Neto ◽  
Amanda E. Ramer-Tait ◽  
...  
Keyword(s):  
Dental Implants ◽  
Dental Implant ◽  
Cytokine Production ◽  
Gingival Crevicular Fluid ◽  
Oral Microbiome ◽  
Anatomical Location ◽  
Implant Placement ◽  
Natural Teeth ◽  
Crevicular Fluid ◽  
Early Healing

ABSTRACT Little is known about longitudinal development of the peri-implant subgingival microbiome and cytokine production as a new sulcus forms after dental implant placement. Therefore, the purpose of this observational study was to evaluate simultaneous longitudinal changes in the oral microbiome and cytokine production in the developing peri-implant sulcus compared to control natural teeth. Four and 12 weeks after implant placement and abutment connection, a dental implant and a natural tooth were sampled in 25 patients for subgingival plaque and gingival crevicular fluid (GCF [around teeth] and peri-implant crevicular fluid [PICF] around implants). DNA from plaque samples was extracted and sequenced using Illumina-based 16S rRNA sequencing. GCF and PICF samples were analyzed using a customized Milliplex human cytokine and chemokine magnetic bead panel. Beta diversity analysis revealed that natural teeth and implants had similar subgingival microbiomes, while teeth had greater alpha diversity than implants. At the genus level, however, few differences were noted between teeth and dental implants over 12 weeks. Specifically, Actinomyces and Selenomonas were significantly elevated around teeth versus dental implants at both 4 weeks and 12 weeks, while Corynebacterium and Campylobacter were significantly elevated only at 4 weeks around teeth. The only difference between PICF and GCF biomarkers was significantly elevated granulocyte-macrophage colony-stimulating factor levels around teeth versus dental implants at the 4-week visit. The subgingival microbiome and cytokine production were similar between teeth and implants during early healing, suggesting that these profiles are driven by the patient following dental implant placement and are not determined by anatomical niche. IMPORTANCE Dental implants are a common treatment option offered to patients for tooth replacement. However, little is known regarding initial colonization of the subgingival microbiome and simultaneous longitudinal cytokine production in humans during the early healing phase following implant placement. We report findings from an in vivo study that assessed initial colonization of the subgingival microbiome and concomitant early cytokine production in a newly formed anatomical space, namely, an implant sulcus. This approach may be useful in future interventional studies to influence dental implant success. Our data showed that the subgingival microbiome and cytokine profile were similar for control natural teeth and dental implants at both 4 and 12 weeks after implant placement. These data suggest that these profiles are driven by the patient and not by anatomical location (i.e., tooth versus dental implant).

scholarly journals Implant Imaging

2013 ◽  
Vol 3 (3) ◽  
pp. 127-132 ◽  
Author(s):  
Ajay R Bhoosreddy ◽  
Seema Bhoosreddy ◽  
Vinayak Umesh Shirsekar
Keyword(s):  
Quality Of Life ◽  
Treatment Planning ◽  
Dental Implants ◽  
Dental Implant ◽  
Diagnostic Information ◽  
Presurgical Evaluation ◽  
Postoperative Assessment ◽  
Implant Placement ◽  
Natural Teeth

ABSTRACT Implants improve the quality of life for patients who are unable to keep their natural teeth, fix acute problems, and give patients the benefit of restorative improvements for a modern lifestyle. The greatest key to successful implant placement is accurate preplacement assessment. Imaging plays a pivotal role in preplacement assessment. Success of dental implant restorations is in part, dependent on adequate diagnostic information about the bony structures of the oral region. This article reviews the applications of different imaging technologies and their diagnostic contribution to presurgical evaluation, treatment planning and postoperative assessment of dental implants. How to cite this article Bhoosreddy AR, Bhoosreddy S, Shirsekar VU. Implant Imaging. J Contemp Dent 2013;3(3): 127-132.

scholarly journals Inpatient Evaluation of Periodontal, Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth

2013 ◽  
Vol 14 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Güliz N Güncü ◽  
Gülüzar Büyüktepe ◽  
Sezen B AÕkin ◽  
Kamer Klllnç ◽  
Celal Tümer ◽  
...  
Keyword(s):  
Dental Implants ◽  
Gingival Crevicular Fluid ◽  
Gingival Index ◽  
Clinical Attachment Level ◽  
Inflammatory Parameters ◽  
Probing Depth ◽  
Natural Teeth ◽  
Fixed Prosthesis ◽  
Crevicular Fluid ◽  
Attachment Level

ABSTRACT Aim The use of endosseous dental implants (DI) has become a successful treatment alternative. However, providing periimplant tissue health and achieving a natural esthetic look are important topics in this treatment. The aim of the present study was to evaluate periodontal and esthetic parameters around DI and natural teeth (NT) and also to analyze myeloperoxidase (MPO) levels in gingival crevicular fluid (GCF) and peri-implant sulcus fluid (PISF). Materials and methods Twenty DI supported fixed prosthesis and contralateral 20 NT were enrolled to the present study. Clinical periodontal parameters (probing depth, clinical attachment level, gingival bleeding time index and gingival index) were recorded and GCF/PISF samples were obtained from mesial (mesiobuccal and mesiolingual) and distal (distobuccal and distolingual) sites of DI and NT. MPO levels were spectrophotometrically determined. Additionally clinical photographs were obtained and esthetical evaluations were performed by using Jemt papilla index. The parameters belong to DI and NT were compared and correlations were evaluated using statistical analysis. Results A total of 40 samples were evaluated. No statistically significant differences were detected between groups in all periodontal parameters and MPO levels from mesial and distal sites. Jemt papilla index scores were slightly higher in NT however, this difference was not statistically significant (p > 0.05). Total PES score were similiar in DI and NT groups. Significant correlations were detected between MPO and gingival index values as expected. Clinical significance Dental implants are satisfactory treatments, they provide patient esthetic natural looking, phonetic and masticatory functions. How to cite this article Güncü GN, Büyüktepe G, AÕkin SB, KlllnH K, Tümer C, Tözüm TF. Inpatient Evaluation of Periodontal Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth. J Contemp Dent Pract 2013;14(1):90-95.

scholarly journals Comparative radiographic and resonance frequency analyses of the peri-implant tissue after dental implants placement using flap and flapless techniques: An experimental study on domestic pigs

2013 ◽  
Vol 70 (6) ◽  
pp. 586-594 ◽  
Author(s):  
Zoran Vlahovic ◽  
Branko Mihailovic ◽  
Zoran Lazic ◽  
Mileta Golubovic
Keyword(s):  
Bone Resorption ◽  
Resonance Frequency ◽  
Dental Implants ◽  
Dental Implant ◽  
Surgical Techniques ◽  
Primary Stability ◽  
Implant Stability ◽  
Domestic Pigs ◽  
Flap Technique ◽  
Implant Placement

Background/Aim. Flapless implant surgery has become very important issue during recent years, mostly thanks to computerization of dentistry and software planning of dental implants placements. The aim of this study was to compare flap and flapless surgical techniques for implant placement through radiographic and radiofrequency analyses. Methods. The experiment was made in five domestic pigs. Nine weeks following domestic pigs teeth extraction, implants were placed, on the right side using surgical technique flap, and flapless on the left side. Digital dental Xrays were applied to determine primary dental implant stability quality (ISQ). At certain intervals, not later than three months, the experimental animals were sacrificed, and just before it, control X-rays were applied to measure dental implants stability. Results. Radiographic analysis showed that peri-implant bone resorption in the first 4 weeks following placement implants with flap and flapless surgical techniques was negligible. After the 3 months, mean value of peri-implant bone resorption of the implants placed using flap technique was 1.86 mm, and of those placed using flapless technique was 1.13 mm. In relation to the primary dental implant stability in the first and second week there was an expected decrease in ISQ values, but it was less expressed in the dental implants placed using the flapless technique. In the third week the ISQ values were increased in the dental implants placed by using both techniques, but the increase in flapless implant placement was higher (7.4 ISQ) than in flap implant placement (1.5 ISQ). The upward trend continued in a 4- week period, and after 3 months the dental implant stability values in the implants placed using flap technique were higher than the primary stability for 7.1 ISQ, and in the implants placed using flapless technique were higher comparing to the primary stability for 10.1 ISQ units. Conclusion. Based on the results of radiographic and resonance frequency analyses it can be concluded that the flapless technique in surgical implants placemat, leads to better results.

Evaluation of the accuracy of a novel surgical guide for flapless dental implant placement for immediate loading in edentulous jaws

2021 ◽  
Author(s):  
Giovanni de Almeida Prado Di Giacomo ◽  
Patrícia Cury ◽  
Airton Moreira da Silva ◽  
Jorge Vicente Lopes da Silva ◽  
Carlos Eduardo Pompeo Souto ◽  
...  
Keyword(s):  
Dental Implants ◽  
Dental Implant ◽  
Selective Laser Sintering ◽  
Apical Region ◽  
Virtual Planning ◽  
Surgical Guide ◽  
Implant Placement ◽  
Surgical Guides ◽  
Edentulous Jaws ◽  
Edentulous Jaw

This study was designed to evaluate the accuracy of a novel computer-designed and selectively laser sintered surgical guide for flapless dental implant placement in the edentulous jaw. Fifty dental implants were placed in 11 patients with at least one totally edentulous jaw. Initially, cone-beam computed tomography (CBCT) was performed in each patient to define the virtual position of the dental implants based on the assessment of bone availability and the proposed dental prosthesis. After virtual planning, 3D surgical guides were printed using selective laser sintering. CBCT was repeated after the surgery, and the pre-and postoperative images were overlapped in the CAD software to compare the planned and actual positions of the dental implants using a one-sample t-test. The mean ± angular standard deviation between the long axes of the planned and final dental implant positions was 4.58 ±2.85 degrees; The linear deviation in the coronal position was  0.87 ± 0.49 mm and  in the apical region of the dental implants was 1.37 ± 0.69 mm. These differences were statistically significant (p <0.001). The proposed modifications reduced the deviations, resulting in an improvement in the technique. We were able to place implants and temporary prostheses using the present protocol, taking into account the differences between the planned and final positions of the dental implants.

scholarly journals Resistance and resilience to experimental gingivitis: a systematic scoping review

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Charifa Zemouri ◽  
Nicholas S. Jakubovics ◽  
Wim Crielaard ◽  
Egija Zaura ◽  
Michael Dodds ◽  
...  
Keyword(s):  
Scoping Review ◽  
Gingival Crevicular Fluid ◽  
Oral Microbiome ◽  
Induction Phase ◽  
Experimental Gingivitis ◽  
Normal Composition ◽  
Baseline Values ◽  
Cystatin Sn ◽  
Crevicular Fluid ◽  
Reporting Data

Abstract Background This systematic scoping review aimed to identify changes in biomarkers of microbiological, immunological and biochemical origin during experimental gingivitis (EG) studies that might indicate resistance and resilience. Methods The term ‘experimental gingivitis’ was run in PubMed from inception to April 11th, 2018. From the 411 studies retrieved, 22 studies were included for this review. Results Studies reporting data on biomarker changes during and after full mouth EG trial were included. Two studies reported findings on changes in biomarkers of microbiological, 12 on immunological and eight on biochemical origin. Changes were reported in the induction phase, and occasionally in the resolution phase. The microbiological composition of both supragingival and subgingival dental plaque changed over the course of EG to a more pathogenic direction, but showed a shift back to a more normal composition. This indicates resilience of the oral microbiome. For immunological biomarkers, it was challenging to retrieve a robust pattern of changes across multiple studies. IL-1β and IL-6 in saliva and in gingival crevicular fluid increased during induction phase and returned in the resolution phase below baseline values. The biochemical parameters cystatin-SN, cystatin-S and lactoferrin in saliva were increased at the end of induction phase, however also here no clear pattern emerged based on all available studies. Conclusions More research is needed to investigate which microbiological, immunological, and biochemical biomarkers can be useful for future investigations into the resistance and resilience of the oral cavity to experimental gingivitis.

scholarly journals Bone Remineralization around Dental Implants following Conservative Treatment after Peri-Implantitis

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Algirdas Puisys ◽  
Viktorija Auzbikaviciute ◽  
Renata Simkunaite-Rizgeliene ◽  
Dainius Razukevicius ◽  
Rokas Linkevicius ◽  
...  
Keyword(s):  
Case Report ◽  
Surgical Treatment ◽  
Conservative Treatment ◽  
Dental Implants ◽  
Dental Implant ◽  
Posterior Part ◽  
Treatment Method ◽  
Color Changes ◽  
Implant Placement ◽  
History Of

The aim of this case report is to show that bone remineralization around dental implants with a history of peri-implantitis is possible after irritant factors are removed and only conservative treatment is performed. Patient came to the clinic after three years of dental implant placement complaining about swelling, sensitivity and gingiva color changes at the posterior part of the maxilla. During radiographic and intraoral examinations peri-implantitis of the #24 implant site was diagnosed. The surgical treatment method was rejected and performed conservative treatment instead. The outcome is promising; periapical radiographs three months later showed bone remineralization as well as stable bone after 10 years. A key clinical message: Bone remineralization around dental implants with a history of peri-implantitis is possible after irritant factors are removed and conservative treatment performed.

scholarly journals Graphene–Chitosan Hybrid Dental Implants with Enhanced Antibacterial and Cell-Proliferation Properties

2020 ◽  
Vol 10 (14) ◽  
pp. 4888 ◽  
Author(s):  
Sunho Park ◽  
Hyeran Kim ◽  
Kyoung Soon Choi ◽  
Min-Kyung Ji ◽  
Sujin Kim ◽  
...  
Keyword(s):  
Dental Implants ◽  
Dental Implant ◽  
Cell Activity ◽  
Functional Abilities ◽  
Tooth Replacement ◽  
Oral Rehabilitation ◽  
Natural Teeth ◽  
New Type ◽  
Implant Treatment ◽  
Effective Design

Dental implants are widely used tooth replacement tools owing to their good oral rehabilitation and reconstruction capacities. Since dental implants are designed as a replacement for natural teeth, multi-functional abilities are desired to achieve successful implant treatment with improved osseointegration through promotion of mammalian cell activity and prevention of bacterial cell activity. In this study, we developed a graphene–chitosan hybrid dental implant (GC hybrid implant) using various concentrations of graphene, which demonstrated the different surface properties including increased wettability and roughness. Importantly, the GC hybrid implant under the optimal condition (i.e., 1% GC hybrid implant) could significantly promote osteoblast proliferation while reducing biofilm formation and bacterial activity. Our study demonstrates the potential of using this GC hybrid implant as a new type of dental implant, which can offer an effective design for the fabrication of advanced dental implants.

Current Update on Potential Clinical Applications of Oral Sensors

2020 ◽  
Vol 18 (5) ◽  
pp. 335-350
Author(s):  
Pandurangan Harikrishnan
Keyword(s):  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Clinical Applications ◽  
Oral Microbiome ◽  
Testing Methods ◽  
Medical Field ◽  
Advanced Imaging ◽  
Non Invasive ◽  
Potential Alternative ◽  
Crevicular Fluid

Dentistry is a specialized medical field involving treatment of oro-facial diseases and also in the diagnosis of systemic diseases through oral manifestations. Advanced imaging and sensing tools are emerging for the diagnosis and monitoring of general health, in-particular the non-invasive testing methods are becoming popular. Saliva and gingival crevicular fluid are evolving as potential alternative diagnostic biofluids. Hence, oral biosensors are emerging for applications like detecting changes in the oral microbiome, monitoring caries, periodontal disease, levels of biomolecules, loading forces during orthodontic treatment and temporomandibular joint (TMJ) movement etc. This review discloses the current literature in the applications of oral sensors in oral and systemic diagnostics, monitoring and therapy.

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