A 3-Day Functional Unloading is Accompanied by an Increase in the TTN Gene Expression in the Rat Soleus Muscle without Changes in Alternative Splicing from Exon 50 to Exon 111

Gene expression is an intricately regulated process that is at the basis of cell differentiation, the maintenance of cell identity and the cellular responses to environmental changes. Alternative splicing, the process by which multiple functionally distinct transcripts are generated from a single gene, is one of the main mechanisms that contribute to expand the coding capacity of genomes and help explain the level of complexity achieved by higher organisms. Eukaryotic transcription is subject to multiple layers of regulation both intrinsic — such as promoter structure — and dynamic, allowing the cell to respond to internal and external signals. Similarly, alternative splicing choices are affected by all of these aspects, mainly through the regulation of transcription elongation, making it a regulatory knob on a par with the regulation of gene expression levels. This review aims to recapitulate some of the history and stepping-stones that led to the paradigms held today about transcription and splicing regulation, with major focus on transcription elongation and its effect on alternative splicing.

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In vitro autoregulation of glucose utilization in rat soleus muscle

Diabetes ◽

10.2337/diabetes.36.9.1041 ◽

1987 ◽

Vol 36 (9) ◽

pp. 1041-1046 ◽

Cited By ~ 9

Author(s):

S. Sasson ◽

D. Edelson ◽

E. Cerasi

Keyword(s):

Soleus Muscle ◽

Glucose Utilization ◽

Rat Soleus Muscle

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The effect of high intensity interval training on SIRT1 and PGC1-α gene expression in soleus muscle of type 2 diabetes in male rat

Medical Journal of Tabriz University of Medical Sciences and Health Services ◽

10.34172/mj.2020.067 ◽

2020 ◽

Vol 42 (4) ◽

pp. 447-455

Author(s):

mahsa mohsenzadeh ◽

fariba aghaei ◽

farah nameni

Keyword(s):

Gene Expression ◽

Type 2 Diabetes ◽

Soleus Muscle ◽

High Intensity ◽

Interval Training ◽

Male Rat ◽

High Intensity Interval Training ◽

High Intensity Interval

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Alternative splicing during mammalian organ development

Nature Genetics ◽

10.1038/s41588-021-00851-w ◽

2021 ◽

Author(s):

Pavel V. Mazin ◽

Philipp Khaitovich ◽

Margarida Cardoso-Moreira ◽

Henrik Kaessmann

Keyword(s):

Gene Expression ◽

Alternative Splicing ◽

Regulatory Networks ◽

Organ Development ◽

Functional Diversification ◽

Mammalian Genomes ◽

Species Comparisons ◽

Time Specific ◽

The Brain ◽

Gene Expression Levels

AbstractAlternative splicing (AS) is pervasive in mammalian genomes, yet cross-species comparisons have been largely restricted to adult tissues and the functionality of most AS events remains unclear. We assessed AS patterns across pre- and postnatal development of seven organs in six mammals and a bird. Our analyses revealed that developmentally dynamic AS events, which are especially prevalent in the brain, are substantially more conserved than nondynamic ones. Cassette exons with increasing inclusion frequencies during development show the strongest signals of conserved and regulated AS. Newly emerged cassette exons are typically incorporated late in testis development, but those retained during evolution are predominantly brain specific. Our work suggests that an intricate interplay of programs controlling gene expression levels and AS is fundamental to organ development, especially for the brain and heart. In these regulatory networks, AS affords substantial functional diversification of genes through the generation of tissue- and time-specific isoforms from broadly expressed genes.

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