Bioelectrical Impedance of the Rat Myocardium and Liver under Chronic Exposure to Doxorubicin

2021 ◽  
Vol 57 (6) ◽  
pp. 1351-1362
Author(s):  
N. L. Kolomeyets ◽  
O. V. Syslonova ◽  
S. L. Smirnova ◽  
E. A. Peshkin ◽  
I. M. Roshchevskaya
Keyword(s):  
Chronic Exposure ◽  
Bioelectrical Impedance ◽  
Rat Myocardium

Development of apical pits in chloride cells of the gills of Pimephales promelas after chronic exposure to acid water

1983 ◽  
Vol 41 ◽  
pp. 462-463
Author(s):  
Richard L. Leino ◽  
Jon G. Anderson ◽  
J. Howard McCormick
Keyword(s):  
Confidence Interval ◽  
Chronic Exposure ◽  
Lake Superior ◽  
Pimephales Promelas ◽  
Chloride Cells ◽  
Fathead Minnows ◽  
Secondary Lamellae ◽  
Untreated Water ◽  
Water Ph ◽  
Flow Through

Groups of 12 fathead minnows were exposed for 129 days to Lake Superior water acidified (pH 5.0, 5.5, 6.0 or 6.5) with reagent grade H2SO4 by means of a multichannel toxicant system for flow-through bioassays. Untreated water (pH 7.5) had the following properties: hardness 45.3 ± 0.3 (95% confidence interval) mg/1 as CaCO3; alkalinity 42.6 ± 0.2 mg/1; Cl- 0.03 meq/1; Na+ 0.05 meq/1; K+ 0.01 meq/1; Ca2+ 0.68 meq/1; Mg2+ 0.26 meq/1; dissolved O2 5.8 ± 0.3 mg/1; free CO2 3.2 ± 0.4 mg/1; T= 24.3 ± 0.1°C. The 1st, 2nd and 3rd gills were subsequently processed for LM (methacrylate), TEM and SEM respectively.Three changes involving chloride cells were correlated with increasing acidity: 1) the appearance of apical pits (figs. 2,5 as compared to figs. 1, 3,4) in chloride cells (about 22% of the chloride cells had pits at pH 5.0); 2) increases in their numbers and 3) increases in the % of these cells in the epithelium of the secondary lamellae.

The Effect of Vitamin A on the Intermittent Nitrogen Dioxide Gas Exposure in Hamsters

1976 ◽  
Vol 34 ◽  
pp. 176-177
Author(s):  
J.C.S. Kim ◽  
M.G. Jourden ◽  
E.S. Carlisle
Keyword(s):  
Electron Microscopy ◽  
Vitamin A ◽  
Nitrogen Dioxide ◽  
Chronic Exposure ◽  
Light And Electron Microscopy ◽  
Specific Effect ◽  
Deficient Diet ◽  
Intermittent Exposure ◽  
Hypovitaminosis A ◽  
Gas Exposure

Chronic exposure to nitrogen dioxide in rodents has shown that injury reaches a maximum after 24 hours, and a reparative adaptive phase follows (1). Damage occurring in the terminal bronchioles and proximal portions of the alveolar ducts in rats has been extensively studied by both light and electron microscopy (1).The present study was undertaken to compare the response of lung tissue to intermittent exposure to 10 ppm of nitrogen dioxide gas for 4 hours per week, while the hamsters were on a vitamin A deficient diet. Ultrastructural observations made from lung tissues obtained from non-gas exposed, hypovitaminosis A animals and gas exposed animals fed a regular commercially prepared diet have been compared to elucidate the specific effect of vitamin A on nitrogen dioxide gas exposure. The interaction occurring between vitamin A and nitrogen dioxide gas has not previously been investigated.

Osmolarity and Artificial Cell Damage in Ischemic Myocardium

1990 ◽  
Vol 48 (3) ◽  
pp. 262-263
Author(s):  
Richard Montione ◽  
Muhammad Ashraf
Keyword(s):  
Cell Damage ◽  
Uranyl Acetate ◽  
Tissue Preservation ◽  
Rat Myocardium ◽  
En Bloc ◽  
Cellular Changes ◽  
Artificial Cell ◽  
Cacodylate Buffer ◽  
Isotonic Buffer ◽  
Perfusion Fixation

Osmolarity of a fixative vehicle has long been known to have an effect on the tissue preservation. An increase in tissue osmolarity occurs in ischemia-damaged tissue and affects the morphology. In this study, we examined cellular changes in ischemic rat myocardium induced by varying fixative toxicity.Rats were sacrificed by decapitation and the hearts immediately removed and retrogradily perfused through the aorta with anoxic Kurbs-Henseleit medium. Hearts were then placed in a bag with a small amount of medium at 37°C for 90 minutes. Hearts were perfusion-fixed using 2% glutaraldehyde in 0.1 M cacodylate buffer pH -7.3 at three osmolarities. The isotonic buffer was adjusted to 311 mOsm/kg using D-manitol. Hypertonic buffers were adjusted to 375 and 400 mOsm/kg. One-half hour after perfusion fixation, the hearts were sliced and cut into small blocks and allowed to fix overnight at 4°C. Blocks were post fixed in osmium, en bloc stained in uranyl acetate, dehydrated in ethanol and embedded in Spurr medium.

Validation of a bioelectrical impedance scale for the estimation of body fat in adolescents

2020 ◽  
Vol 19 (5) ◽  
pp. 369
Author(s):  
Andreia Pelegrini ◽  
André De Araújo Pinto ◽  
Hector Cris Colares De Angelo ◽  
Gaia Salvador Claumann ◽  
Diego Augusto Santos Silva ◽  
...  
Keyword(s):  
Body Fat ◽  
Bioelectrical Impedance ◽  
Air Displacement Plethysmography ◽  
Overweight Adolescents ◽  
The Absolute ◽  
Absolute Measurements

Several different instruments available on the market have been used for the estimation of body fat. However, many of these instruments have not been compared with reference criteria to verify their true accuracy. This study aimed to verify the validity of a bioelectrical impedance scale (OMRON-514C) for the estimation of body fat. Forty-four overweight adolescents (25 females) participated in this study, with an average age of 12.3 ± 1.1 years. All were submitted to body fat evaluations by air displacement plethysmography and bioelectrical impedance. Higher values of relative and absolute body fat were estimated by bioelectrical impedance compared to plethysmography (p < 0.05). There was no correlation between the relative body fat measurements between the two methods (r = 0.185; p = 0.228). The absolute measurements of body fat were correlated (r = 0.497, p = 0.001). Both in the measurements of relative (p= 0.034) and absolute body fat (p = 0.021), the bioelectrical impedance overestimated the measured values. Thus, in adolescents with characteristics similar to the present study, the estimate of body fat by the bioelectrical impedance (OMRON-514C) should be used with caution.Keywords: plethysmography, bioelectrical impedance, adolescents, overweight.

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