Chromatographic Separation and Determination of Anthocyanins under Conditions of Reversed Phase Chromatography, When Used As Mobile Phases of Acetonitrile–Formic Acid/Phosphoric Acid–Water Systems

2021 ◽  
Vol 95 (8) ◽  
pp. 1729-1734
Author(s):  
V. I. Deineka ◽  
E. Yu. Oleinits ◽  
Y. Yu. Kulchenko ◽  
I. P. Blinova ◽  
L. A. Deineka
Keyword(s):  
Phosphoric Acid ◽  
Formic Acid ◽  
Chromatographic Separation ◽  
Water Systems ◽  
Reversed Phase ◽  
Acid Water ◽  
Reversed Phase Chromatography ◽  
Mobile Phases

Determination of isotope fractionation of Cr(iii) during oxidation by LC/low-resolution MC-ICPMS

2020 ◽  
Vol 35 (3) ◽  
pp. 560-566
Author(s):  
Jakub Karasiński ◽  
Cuc Thi Nguyen-Marcińczyk ◽  
Marcin Wojciechowski ◽  
Ewa Bulska ◽  
Ludwik Halicz
Keyword(s):  
Inductively Coupled Plasma ◽  
Isotope Fractionation ◽  
Isotopic Analysis ◽  
Reversed Phase ◽  
Ion Pair ◽  
Reversed Phase Chromatography ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Sensitive Method

A robust and sensitive method for the precise isotopic analysis of 53Cr/50Cr during Cr(iii) oxidation by coupling ion pair reversed-phase chromatography and Multicollector Inductively Coupled Plasma Mass Spectrometry (MC-ICPMS) is presented.

scholarly journals Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS

2020 ◽  
Vol 10 (5) ◽  
pp. 1713 ◽  
Author(s):  
Chang-Seob Seo ◽  
Hyeun-Kyoo Shin
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Coefficient Of Determination ◽  
Photodiode Array Detection ◽  
Mobile Phases ◽  
Photodiode Array ◽  
Array Detection ◽  
Aqueous Formic Acid

Yeonkyopaedok-san is a traditional Korean medicine used in the early treatment of boils. In the present study, its 12 marker components for quality control were determined using high-performance liquid chromatography (HPLC) with photodiode array detection and ultra-performance liquid chromatography–mass spectrometry with tandem mass spectrometry (UPLC–MS/MS). The investigated 12 marker components of Yeonkyopaedok-san were as follows: 3-caffeoylquinic acid, cimifugin 7-glucoside, liquiritin apioside, ferulic acid, narirutin, 5-O-methylvisammioside, naringin, neohesperidin, oxypeucedanin hydrate, arctigenin, glycyrrhizic acid, and 6-gingerol. The analytical column used for the separation of the 12 marker analytes in Yeonkyopaedok-san was a Waters SunFire C18 column (4.6 mm × 250 mm, 5 μm). The two mobile phases used were 0.1% (v/v) aqueous formic acid and 0.1% (v/v) formic acid in acetonitrile. In the UPLC–MS/MS analysis, all components were separated using a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm). The two mobile phases used were 0.1% (v/v) aqueous formic acid and acetonitrile. The coefficient of determination of the calibration curves in both analysis systems showed good linearity (>0.99). The amounts of the 12 marker components in Yeonkyopaedok-san determined using HPLC–photodiode array detection and UPLC–MS/MS analyses were found to be 0.14–9.00 mg/g and 2.35–853.11 μg/g, respectively.

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