Effect of Tryptophan and Kynurenine on Cell Proliferation in Tissue Culture of the Cerebral Cortex in Young and Old Rats

2019 ◽  
Vol 9 (2) ◽  
pp. 186-189 ◽  
Author(s):  
N. I. Chalisova ◽  
P. N. Ivanova ◽  
E. S. Zalomaeva ◽  
E. A. Nikitina ◽  
L. S. Kozina
2020 ◽  
Vol 10 (3) ◽  
pp. 282-286
Author(s):  
N. I. Chalisova ◽  
G. A. Rizhak ◽  
M. A. Zayitseva ◽  
M. L. Alexandrova ◽  
A. A. Krasnova ◽  
...  

2013 ◽  
Vol 3 (1) ◽  
pp. 77-79 ◽  
Author(s):  
N. I. Chalisova ◽  
E. A. Kontsevaya ◽  
A. N. Zhekalov ◽  
D. A. Sinyachkin

Author(s):  
V. B. Dolgo-Saburov ◽  
N. I. Chalisova ◽  
L. V. Lyanginen ◽  
E. S. Zalomaeva

In an organotypic culture, an investigation was conducted into combined effects of cyclophosphamide DNA as synthesis inhibitor used to model a resorptive action of mustard gas, and cortexin polypeptide or each of 20 encoded amino acids on the development of cell proliferation in cerebral cortex explants of the rat. The combined administration of cyclophosphamide together with cortexin or with each of the 20 encoded amino acids, except glycine, showed suppression of the cytostatic agent inhibitory effect. Thus, cortexin and amino acids have a protective effect on cell proliferation in the tissue culture of the central nervous system under the action of mustardlike substances.


1998 ◽  
Vol 544 ◽  
Author(s):  
D. B. Haddow ◽  
R. M. France ◽  
R. D. Short ◽  
S. Macneil ◽  
R. A. Dawson

AbstractHuman keratinocytes have been cultured on plasma co-polymers (PCPs), self assembled monolayers (SAMs), tissue culture poly(styrene) (TCPS) and collagen I. The degree of keratinocyte attachment was measured over 24 hours and cell proliferation and growth monitored over 7 days using optical microscopy and DNA concentrations. Cell attachment and proliferation and growth on the PCP surfaces were compared with 2 self assembled monolayer (SAM) systems. PCP surfaces containing carboxylic acid functionalities promoted keratinocyte attachment, with optimum attachment levels seen on surfaces containing less than 5% acid groups. The level of attachment on these surfaces was comparable to that seen on collagen I, a preferred substratum for the culturing of keratinocytes. After several days in culture the cells were well attached and proliferative. Keratinocytes attached well to acidterminated SAMs but attached poorly to methyl-terminated SAMs.


The paper describes the role played by mass spectrometry in structural studies of molluscicidal saponins from a plant; the adducts formed between 7,12-dimethylbenz[a]anthracene and nucleic acids upon tissue culture with rat liver; and the G2 factor, the first natural mitotic hormone that regulates cell proliferation.


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