Effect of Dilantin Sodium Analogues on Cell Proliferation in Tissue Culture.

1961 ◽  
Vol 106 (1) ◽  
pp. 205-207 ◽  
Author(s):  
W. G. Shafer
Keyword(s):  
Cell Proliferation ◽  
Tissue Culture

PROTECTIVE EFFECT OF POLYPEPTIDE AND AMINO ACIDS ON THE DEVELOPMENT OF THE NERVOUS TISSUE CULTURE IN THE PRESENCE OF CYCLOPHOSPHAMIDE

2017 ◽  
pp. 22-26
Author(s):  
V. B. Dolgo-Saburov ◽  
N. I. Chalisova ◽  
L. V. Lyanginen ◽  
E. S. Zalomaeva
Keyword(s):  
Amino Acids ◽  
Cell Proliferation ◽  
Tissue Culture ◽  
Protective Effect ◽  
Organotypic Culture ◽  
Inhibitory Effect ◽  
Mustard Gas ◽  
Synthesis Inhibitor ◽  
Combined Administration ◽  
The Central Nervous System

In an organotypic culture, an investigation was conducted into combined effects of cyclophosphamide DNA as synthesis inhibitor used to model a resorptive action of mustard gas, and cortexin polypeptide or each of 20 encoded amino acids on the development of cell proliferation in cerebral cortex explants of the rat. The combined administration of cyclophosphamide together with cortexin or with each of the 20 encoded amino acids, except glycine, showed suppression of the cytostatic agent inhibitory effect. Thus, cortexin and amino acids have a protective effect on cell proliferation in the tissue culture of the central nervous system under the action of mustardlike substances.

Comparison of Proliferation and Growth of Human Keratinocytes on Plasma CO-Polymers of Acrylic acid/1,7- Octadiene and Self Assembled Monolayers

1998 ◽  
Vol 544 ◽  
Author(s):  
D. B. Haddow ◽  
R. M. France ◽  
R. D. Short ◽  
S. Macneil ◽  
R. A. Dawson
Keyword(s):  
Cell Proliferation ◽  
Tissue Culture ◽  
Carboxylic Acid ◽  
Cell Attachment ◽  
Human Keratinocytes ◽  
Collagen I ◽  
Self Assembled Monolayers ◽  
Self Assembled Monolayer ◽  
Assembled Monolayers ◽  
Self Assembled

AbstractHuman keratinocytes have been cultured on plasma co-polymers (PCPs), self assembled monolayers (SAMs), tissue culture poly(styrene) (TCPS) and collagen I. The degree of keratinocyte attachment was measured over 24 hours and cell proliferation and growth monitored over 7 days using optical microscopy and DNA concentrations. Cell attachment and proliferation and growth on the PCP surfaces were compared with 2 self assembled monolayer (SAM) systems. PCP surfaces containing carboxylic acid functionalities promoted keratinocyte attachment, with optimum attachment levels seen on surfaces containing less than 5% acid groups. The level of attachment on these surfaces was comparable to that seen on collagen I, a preferred substratum for the culturing of keratinocytes. After several days in culture the cells were well attached and proliferative. Keratinocytes attached well to acidterminated SAMs but attached poorly to methyl-terminated SAMs.

Applications of mass spectrometry in structural studies of bioactive compounds

1979 ◽  
Vol 293 (1400) ◽  
pp. 3-11
Keyword(s):  
Mass Spectrometry ◽  
Cell Proliferation ◽  
Tissue Culture ◽  
Nucleic Acids ◽  
Bioactive Compounds ◽  
Rat Liver ◽  
Structural Studies

The paper describes the role played by mass spectrometry in structural studies of molluscicidal saponins from a plant; the adducts formed between 7,12-dimethylbenz[a]anthracene and nucleic acids upon tissue culture with rat liver; and the G2 factor, the first natural mitotic hormone that regulates cell proliferation.

Effect of Tryptophan and Kynurenine on Cell Proliferation in Tissue Culture of the Cerebral Cortex in Young and Old Rats

2019 ◽  
Vol 9 (2) ◽  
pp. 186-189 ◽  
Author(s):  
N. I. Chalisova ◽  
P. N. Ivanova ◽  
E. S. Zalomaeva ◽  
E. A. Nikitina ◽  
L. S. Kozina
Keyword(s):  
Cell Proliferation ◽  
Tissue Culture ◽  
Cerebral Cortex ◽  
Old Rats

Improvement of in situ Follicular Activation and Early Development in Cryopreserved Human Ovarian Cortical Tissue by Co-Culturing with Mesenchymal Stem Cells

2019 ◽  
Vol 208 (1-2) ◽  
pp. 48-58
Author(s):  
Marzieh Hosseini ◽  
Saghar Salehpour ◽  
Marefat Ghaffari Novin ◽  
Zahra Shams Mofarahe ◽  
Mohammad-Amin Abdollahifar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Tissue Culture ◽  
Mesenchymal Stem Cells ◽  
Ovarian Tissue ◽  
Follicular Development ◽  
Cortical Tissue ◽  
Culture Systems ◽  
The Impact

Follicular loss and tissue degeneration are great challenges in ovarian tissue culture systems. Mesenchymal stem cells (MSC) secrete a cocktail of growth factors and cytokines which supports adjacent cells and tissues. The aim of the current study was to investigate the impact of human bone marrow (hBM)-MSC, as co-culture cells, on human follicular development in ovarian cortical tissue (OCT) culture. For this purpose, warmed OCT fragments were co-cultured with hBM-MSC for 8 days and compared to monocultured OCT. During the culture period, ovarian follicle survival and development in the OCT were evaluated using histological observation, follicular developmental-related genes expression, and estradiol production. Furthermore, cell proliferation and apoptosis were assessed. The results showed that there were no significant differences in conserved ovarian follicles with a normal morphology between the two groups. However, the percentage of developing follicles, as well as follicular developmental gene expression, significantly increased in the co-culture group compared to the monoculture group. On the other hand, compared with the monoculture group, the co-culture group demonstrated a significant increase in cell proliferation, indicated by Ki67 gene expression, as well as a dramatic decrease in apoptotic cell percentage, revealed by TUNEL assay. These findings indicated that co-culturing of hBM-MSC with OCT could improve follicular activation and early follicular development in human ovarian tissue culture systems.

Cell proliferation and protein synthesis (keratinization) in bovine hoof during the onset and recovery from clinical lameness

1998 ◽  
Vol 1998 ◽  
pp. 12-12
Author(s):  
A.J. MacCallum ◽  
K.A.K. Hendry ◽  
S. Robertson ◽  
C.J. Wilde ◽  
C.H. Knight
Keyword(s):  
Cell Proliferation ◽  
Tissue Culture ◽  
Protein Synthesis ◽  
Dairy Cows ◽  
Economic Loss ◽  
Culture Technique ◽  
Measuring Cell ◽  
Tissue Culture Technique ◽  
Production Economic ◽  
Bovine Hoof

Lameness is a major cause of lost production, economic loss and compromised welfare in dairy cows. The majority of bovine lameness results from damage to the hoof, culminating in solear lesions and inflammation of the laminae (laminitis). Management factors predisposing to laminitis have been identified, but little is known about the biology of hoof development. Impaired keratinization has been linked histologically with subsequent laminitis, but factors regulating keratinization are unknown. We have developed a tissue culture technique for measuring cell proliferation and keratinization in hoof explants obtained by biopsy.

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