A9.10 Synovial activation drives anti-inflammatory effects of adipose-derived stem cells after local administration in experimental oa which is reflected by s100a8/a9 levels in the serum

2014 ◽  
Vol 73 (Suppl 1) ◽  
pp. A95.3-A96
Author(s):  
P L E M van Lent ◽  
R F Schelbergen ◽  
S van Daalen ◽  
M C ter Huurne ◽  
A B Blom ◽  
...  
Keyword(s):  
Stem Cells ◽  
Local Administration ◽  
Adipose Derived Stem Cells ◽  
Anti Inflammatory

scholarly journals Oxidant therapy improves adipogenic differentiation of adipose-derived stem cells in human wound healing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Christian Ploner ◽  
Tina Rauchenwald ◽  
Catherine E. Connolly ◽  
Karin Joehrer ◽  
Johannes Rainer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Granulation Tissue ◽  
Adipogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Activated Macrophages ◽  
Fat Tissue ◽  
Differentiation Potential ◽  
Anti Inflammatory ◽  
Wound Tissue

Abstract Background Adipose-derived stem cells (ASC) and adipocytes are involved in numerous physiological and pathophysiological conditions, which have been extensively described in subcutaneous and visceral fat depots over the past two decades. However, much less is known about ASC and adipocytes outside classical fat tissue depots and their necessity in tissue remodeling after injury. Therefore, we investigated the etiology of adipocytes in human granulation tissue and define their possible role wound healing. Methods Identification of human wound tissue adipocytes was determined by immunohistochemical staining of granulation tissue sections from patients undergoing surgical debridement. Stromal cell fractions from granulation tissue and subcutaneous fat tissue were generated by collagenase type II-based protocols. Pro- and anti-inflammatory wound bed conditions were mimicked by THP1- and CD14+ monocyte-derived macrophage models in vitro. Effects of macrophage secretome on ASC differentiation and metabolism were determined by immunoblotting, flow cytometry, and microscopy assessing early and late adipocyte differentiation states. Functional rescuing experiments were conducted by lentiviral transduction of wildtype PPARG, IL1RA, and N-chlorotaurine (NCT) treatment. Results Single and clustered adipocyte populations were detected in 11 out of 13 granulation tissue specimens and single-cell suspensions from granulation tissue showed adipogenic differentiation potential. Pro-inflammatory signaling by IFNG/LPS-stimulated macrophages (M (IFNG/LPS)) inhibited the maturation of lipid droplets in differentiated ASC. In contrast, anti-inflammatory IL4/IL13-activated macrophages (M (IL4/IL13)) revealed minor effects on adipocyte development. The M (IFNG/LPS)-induced phenotype was associated with a switch from endogenous fatty acid synthesis to glycolysis-dominated cell metabolism and increased pro-inflammatory cytokine production. Impaired adipogenesis was associated with increased, but seemingly non-functional, CEBPB levels, which failed to induce downstream PPARG and CEBPA. Neither transgenic PPARG overexpression, nor inhibition of IL1B was sufficient to rescue the anti-adipogenic effects induced by IFNG/LPS-activated macrophages. Instead, macrophage co-treatment during stimulation with NCT, a mild oxidant produced by activated granulocytes present in human wounds in vivo, significantly attenuated the anti-adipogenic effects. Conclusions In conclusion, the appearance of adipocytes in wound tissue indicates a prevailing anti-inflammatory environment that could be promoted by NCT treatment and may be associated with improved healing outcomes. Graphical abstract

scholarly journals Adipose Tissue-Derived Stem Cells: Immunomodulatory Effects and Therapeutic Potential

Physiology ◽  
2020 ◽  
Vol 35 (2) ◽  
pp. 125-133 ◽  
Author(s):  
Sara Al-Ghadban ◽  
Bruce A. Bunnell
Keyword(s):  
Stem Cells ◽  
Therapeutic Potential ◽  
Mechanisms Of Action ◽  
Adipose Derived Stem Cells ◽  
Immunomodulatory Effects ◽  
Graft Versus Host ◽  
Cell Lineages ◽  
Pathological Conditions ◽  
Multiple Cell ◽  
Anti Inflammatory

Adipose-derived stem cells (ASCs) can self-renew and differentiate along multiple cell lineages. ASCs are also potently anti-inflammatory due to their inherent ability to regulate the immune system by secreting anti-inflammatory cytokines and growth factors that play a crucial role in the pathology of many diseases, including multiple sclerosis, diabetes mellitus, Crohn’s, SLE, and graft-versus-host disease. The immunomodulatory effects and mechanisms of action of ASCs on pathological conditions are reviewed here.

scholarly journals Immunomodulatory Properties of Adipose-Derived Stem Cells Treated with 5-Azacytydine and Resveratrol on Peripheral Blood Mononuclear Cells and Macrophages in Metabolic Syndrome Animals

2018 ◽  
Vol 7 (11) ◽  
pp. 383 ◽  
Author(s):  
Katarzyna Kornicka ◽  
Agnieszka Śmieszek ◽  
Agnieszka Węgrzyn ◽  
Michael Röcken ◽  
Krzysztof Marycz
Keyword(s):  
Metabolic Syndrome ◽  
Stem Cells ◽  
Mononuclear Cells ◽  
Adipose Derived Stem Cells ◽  
Peripheral Blood Mononuclear ◽  
Raw264.7 Macrophages ◽  
Tnf Α ◽  
Blood Mononuclear Cells ◽  
Immunomodulatory Properties ◽  
Anti Inflammatory

Endocrine disorders, including equine metabolic syndrome (EMS), are a serious issue in veterinary medicine and horse breeding. Furthermore, EMS was shown to affect the cytophysiological properties of adipose-derived stem cells, reducing their therapeutic potential. However, it was shown that those cells can be rejuvenated while using a combination of two chemicals: 5-azacytydine (AZA) and resveratrol (RES). In the present study, we decided to evaluate the immunomodulatory properties of AZA/RES-treated adipose-derived stem cells (ASC) isolated from EMS horses (ASCEMS). Thus, we co-cultured ASC with peripheral blood mononuclear cells (PBMC) and RAW264.7 macrophages. Most attention was placed on regulatory T lymphocytes (TREG), as well as the messenger RNA (mRNA) and protein levels of several cytokines (tumor necrosis factor α (TNF-α), interleukin (IL)-6, IL-10, and IL-1β). Moreover, we also investigated the expression of genes related to auto- and mitophagy in both PBMCs and ASCs. PBMCs were obtained from healthy and EMS-suffering individuals and were co-cultured with ASCs that were isolated from healthy and EMS horses cultured in control conditions and with AZA/RES. We discovered that cells treated with AZA/RES increase the TREG number while co-cultured with PBMCs. Moreover, the co-culture of PBMCs with AZA/RES-treated ASCEMS induced mitophagy in PBMCs. Furthermore, ASCEMS pre-treated with AZA/RES displayed anti-inflammatory properties, as decreased levels of TNF-α, nitric oxide (NO), and IL-6 were observed in those cells in comparison with their untreated counterparts in the co-culture with RAW264.7 macrophages. In summary, we demonstrated that ASCEMS treated with AZA/RES displayed increased anti-inflammatory properties, and was able to regulate and activate the TREG-related anti-inflammatory response.

Osteoplasty in Disseminated Calvarial Defects Treated with Autologous Adipose-Derived Stem Cells and Autologous Fibrin Glue: Case Report

Skull Base ◽  
2005 ◽  
Vol 15 (S 2) ◽  
Author(s):  
Stefan Lendeckel ◽  
A. Jödicke ◽  
P. Christophis ◽  
K. Heidinger ◽  
H.-P. Howaldt
Keyword(s):  
Stem Cells ◽  
Case Report ◽  
Fibrin Glue ◽  
Adipose Derived Stem Cells ◽  
Autologous Fibrin Glue ◽  
Calvarial Defects
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