scholarly journals THU0513 WHOLE BLOOD CELLS FROM PATIENTS WITH SYSTEMIC JUVENILE IDIOPATHIC ARTHRITIS (SJIA) IN CLINICAL INACTIVE DISEASE DISPLAY A DYSREGULATED RESPONSE TO TLR-4 STIMULATION

2019 ◽  
Author(s):  
Antonia Pascarella ◽  
Manuela Pardeo ◽  
Ivan Caiello ◽  
Claudia Bracaglia ◽  
Marianna Rossi ◽  
...  
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Whole Blood ◽  
Blood Cells ◽  
Systemic Juvenile Idiopathic Arthritis ◽  
Inactive Disease ◽  
Whole Blood Cells

scholarly journals Identification of Cytokines in Whole Blood for Differential Diagnosis of Tuberculosis versus Pneumonia

2010 ◽  
Vol 17 (5) ◽  
pp. 771-777 ◽  
Author(s):  
Wen-Lin Su ◽  
Wann-Cherng Perng ◽  
Ching-Hui Huang ◽  
Cheng-Yu Yang ◽  
Chin-Pyng Wu ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Cutoff Point ◽  
Interleukin 12 ◽  
Operating Characteristics ◽  
Percent Change ◽  
Ifn Γ ◽  
Sensitivity Specificity ◽  
Whole Blood Cells ◽  
Stimulation Of

ABSTRACT Differentiating tuberculosis (TB) from pneumonia remains a challenge. We evaluated the cytokine profiles of whole blood cells from patients with TB (n = 38) or pneumonia (n = 30) and from healthy individuals (n = 30) before and after stimulating cells with ESAT-6 or lipopolysaccharide (LPS). When the percent change in the levels of gamma interferon (IFN-γ) after stimulation with ESAT-6 was used in receiver operating characteristics (ROC) analysis (a graphic method to determine the diagnostic accuracy of a test) to identify a patient with TB, the area under the curve (AUC) was 90.4%, and a cutoff point of a 3.59% change produced a corresponding sensitivity, specificity, and accuracy of over 80%. When the change in IFN-γ after stimulation of blood cells with LPS was used to identify a patient with pneumonia, the AUC reached 89.1%, and a cutoff point of 3.59% produced a sensitivity, specificity, and accuracy of approximately 80% each. When the change in interleukin-12 (IL-12) after stimulation of blood cells with LPS was selected to define a patient with pneumonia, the AUC was 85.2%, and a cutoff point of 2.08% gave a sensitivity, specificity, and accuracy of 80.0%, 78.9%, and 79.4%, respectively. We conclude that the percent change in IFN-γ after stimulation of whole blood cells with ESAT-6 may differentiate patients with TB from patients with pneumonia. The percent change in IFN-γ and IL-12 after LPS stimulation of whole blood cells could differentiate patients with pneumonia from patients with TB.

scholarly journals Synthesis of the 3,5-diphenyl-1H-pyrazole and cytogenetic and oxidative alterations after exposure of cultured human whole blood cells

2017 ◽  
Vol 3 (1) ◽  
pp. 1344115 ◽  
Author(s):  
Esvet Akbas ◽  
Fatih Caglar Celikezen ◽  
Hasan Turkez ◽  
Ozlem Ozdemir ◽  
Adem Ruzgar ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Human Whole Blood ◽  
Whole Blood Cells

scholarly journals Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Lada Živković ◽  
Vladan Bajić ◽  
Dijana Topalović ◽  
Marija Bruić ◽  
Biljana Spremo-Potparević
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Natural Products ◽  
Whole Blood ◽  
Blood Cells ◽  
Statistical Significance ◽  
Individual Treatment ◽  
Alternative Source ◽  
Whole Blood Cells ◽  
Antigenotoxic Effects

The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.

Evaluating the genotoxic damage in bovine whole blood cells in vitro after exposure to thiacloprid

2019 ◽  
Vol 61 ◽  
pp. 104616 ◽  
Author(s):  
Martina Galdíková ◽  
Beáta Holečková ◽  
Katarína Šiviková ◽  
Viera Schwarzbacherová ◽  
Simona Koleničová
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Genotoxic Damage ◽  
Whole Blood Cells

Effect of lipopolysaccharide structure on functional response of whole blood cells

Immunobiology ◽  
2021 ◽  
Vol 226 (1) ◽  
pp. 152030
Author(s):  
Svetlana V. Zubova ◽  
Sergey V. Grachev ◽  
Isabella R. Prokhorenko
Keyword(s):  
Functional Response ◽  
Whole Blood ◽  
Blood Cells ◽  
Whole Blood Cells

Beta-galactosylceramide increases and sulfatide decreases cytokine and chemokine production in whole blood cells

2004 ◽  
Vol 91 (2-3) ◽  
pp. 205-211 ◽  
Author(s):  
A. Roeske-Nielsen ◽  
P. Fredman ◽  
J.E. Mansson ◽  
K. Bendtzen ◽  
K. Buschard
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Chemokine Production ◽  
Whole Blood Cells

Cytokine production by whole blood cells of the reconvalescents of community-acquired pneumonia under the influence of low-intensity microwave irradiation

10.12737/5025 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Никифоров ◽  
V. Nikiforov ◽  
Терехов ◽  
I. Terekhov ◽  
Хадарцев ◽  
...  
Keyword(s):  
Microwave Radiation ◽  
Whole Blood ◽  
Blood Cells ◽  
Cytokine Production ◽  
Cell Activity ◽  
Community Acquired Pneumonia ◽  
Tnf Alpha ◽  
Toll Like Receptor ◽  
Low Intensity ◽  
Whole Blood Cells

The study discusses the effect on the functional state of the whole blood cells of low-intensity microwave radiation. On the model of intercellular interactions in the conditions of spontaneous cell activity and by stimulating cells complex mitogen, the influence of microwave radiation 1000 MHz on production by whole blood cells interleukins: IL-1β, 2, 6, 8, 10, 12, 13, 17A, TNF-alpha, IFN-g, RAIL-1, and soluble forms of toll-like receptor 1, 2, 4, 6 types was studied. The research results show that a single 45 minutes of exposure of microwave radiation of 0.05 PPM microwatt/cm2 has the ability to enhance mitogen-stimulated production of RAIL-1 by 38,7% (p=0.03), to reduce the induced production of IL-1β by 26,3% (p=0,037), IL-8 by 56,2% (p=0.022) and to increase oppressed segment complex mitogen production of IL-10 by 27,8% (p=0,041). The microwave radiation increased spontaneous and mitogen-stimulated expression of TLR, especially TLR1 during the initial low level.

Inflammatory gene expression in whole blood cells after EPA vs. DHA supplementation: Results from the ComparED study

2017 ◽  
Vol 257 ◽  
pp. 116-122 ◽  
Author(s):  
Cécile Vors ◽  
Janie Allaire ◽  
Johanne Marin ◽  
Marie-Claude Lépine ◽  
Amélie Charest ◽  
...  
Keyword(s):  
Gene Expression ◽  
Whole Blood ◽  
Blood Cells ◽  
Inflammatory Gene Expression ◽  
Inflammatory Gene ◽  
Whole Blood Cells
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