Influence of habitual dietary fibre intake on the responsiveness of the gut microbiota to a prebiotic: protocol for a randomised, double-blind, placebo-controlled, cross-over, single-centre study

AbstractDysbiotic gut microbiota have been implicated in human disease. Diet-based therapeutic strategies have been used to manipulate the gut microbiota towards a more favourable profile. However, it has been demonstrated that large inter-individual variability exists in gut microbiota response to a dietary intervention. The primary objective of this study was to investigate whether habitually low dietary fibre (LDF)v. high dietary fibre (HDF) intakes influence gut microbiota response to an inulin-type fructan prebiotic. In this randomised, double-blind, placebo-controlled, cross-over study, thirty-four healthy participants were classified as LDF or HDF consumers. Gut microbiota composition (16S rRNA bacterial gene sequencing) and SCFA concentrations were assessed following 3 weeks of daily prebiotic supplementation (Orafti®Synergy 1; 16 g/d) or placebo (Glucidex®29 Premium; 16 g/d), as well as after 3 weeks of the alternative intervention, following a 3-week washout period. In the LDF group, the prebiotic intervention led to an increase inBifidobacterium(P=0·001). In the HDF group, the prebiotic intervention led to an increase inBifidobacterium(P<0·001) andFaecalibacterium(P=0·010) and decreases inCoprococcus(P=0·010), Dorea(P=0·043) andRuminococcus(Lachnospiraceae family) (P=0·032). This study demonstrates that those with HDF intakes have a greater gut microbiota response and are therefore more likely to benefit from an inulin-type fructan prebiotic than those with LDF intakes. Future studies aiming to modulate the gut microbiota and improve host health, using an inulin-type fructan prebiotic, should take habitual dietary fibre intake into account.

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Duration of hemodynamic action of intravenous 24 h infusion of levosimendan in congestive heart failure - a randomised, double-blind, placebo-controlled single centre study

Journal of Cardiac Failure ◽

10.1016/j.cardfail.2004.06.217 ◽

2004 ◽

Vol 10 (4) ◽

pp. S77

Author(s):

J. Lilleberg ◽

M. Laine ◽

T. Palkama ◽

P. Pohjanjousi ◽

M. Kivikko ◽

...

Keyword(s):

Heart Failure ◽

Congestive Heart Failure ◽

Single Centre ◽

Double Blind ◽

Double Blind Placebo ◽

Centre Study ◽

Single Centre Study

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TCT-162: Ticagrelor Enhances Adenosine-induced Coronary Vasodilatory Responses in Humans—a Randomised, Double-Blind, Placebo-Controlled, Crossover, Single Centre Study Using Transthoracic Color Doppler Echocardiography

Journal of the American College of Cardiology ◽

10.1016/j.jacc.2011.10.168 ◽

2011 ◽

Vol 58 (20) ◽

pp. B43

Keyword(s):

Doppler Echocardiography ◽

Color Doppler ◽

Single Centre ◽

Color Doppler Echocardiography ◽

Double Blind ◽

Double Blind Placebo ◽

Centre Study ◽

Single Centre Study

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Association of dietary fibre intake and gut microbiota in adults

British Journal Of Nutrition ◽

10.1017/s0007114518002465 ◽

2018 ◽

Vol 120 (9) ◽

pp. 1014-1022 ◽

Cited By ~ 20

Author(s):

Daniel Lin ◽

Brandilyn A. Peters ◽

Charles Friedlander ◽

Hal J. Freiman ◽

James J. Goedert ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Risk ◽

Gut Microbiota ◽

Dietary Fibre ◽

Meta Analysis ◽

Microbiota Composition ◽

Fibre Intake ◽

Dietary Fibre Intake ◽

The Relationship ◽

Gut Microbiota Composition

AbstractIncreasing evidence indicates that gut microbiota may influence colorectal cancer risk. Diet, particularly fibre intake, may modify gut microbiota composition, which may affect cancer risk. We investigated the relationship between dietary fibre intake and gut microbiota in adults. Using 16S rRNA gene sequencing, we assessed gut microbiota in faecal samples from 151 adults in two independent study populations: National Cancer Institute (NCI), n 75, and New York University (NYU), n 76. We calculated energy-adjusted fibre intake based on FFQ. For each study population with adjustment for age, sex, race, BMI and smoking, we evaluated the relationship between fibre intake and gut microbiota community composition and taxon abundance. Total fibre intake was significantly associated with overall microbial community composition in NYU (P=0·008) but not in NCI (P=0·81). In a meta-analysis of both study populations, higher fibre intake tended to be associated with genera of class Clostridia, including higher abundance of SMB53 (fold change (FC)=1·04, P=0·04), Lachnospira (FC=1·03, P=0·05) and Faecalibacterium (FC=1·03, P=0·06), and lower abundance of Actinomyces (FC=0·95, P=0·002), Odoribacter (FC=0·95, P=0·03) and Oscillospira (FC=0·96, P=0·06). A species-level meta-analysis showed that higher fibre intake was marginally associated with greater abundance of Faecalibacterium prausnitzii (FC=1·03, P=0·07) and lower abundance of Eubacterium dolichum (FC=0·96, P=0·04) and Bacteroides uniformis (FC=0·97, P=0·05). Thus, dietary fibre intake may impact gut microbiota composition, particularly class Clostridia, and may favour putatively beneficial bacteria such as F. prausnitzii. These findings warrant further understanding of diet–microbiota relationships for future development of colorectal cancer prevention strategies.

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