Relation between Helicobacter pylori infection and gastrointestinal symptoms and syndromes

Background—Helicobacter pylori is a human pathogen that colonises the gastric mucosa and causes permanent gastric inflammation.Aims—To assess the symptoms of H pylori infection in an adult unselected population.Subjects—A random sample of 3589 adult Danes who were examined in 1982 and 1987 (n=2987).Methods—Abdominal symptoms within the preceding year were recorded at both attendances. Circulating IgG antibodies against H pylori in serum samples drawn in 1982 were measured by using in-house indirect enzyme linked immunosorbent assays (ELISA).Results—People with increased levels of IgG antibodies to H pylori were more likely than uninfected individuals to report heartburn (odds ratio (OR) = 1.26, 95% confidence interval (CI) 1.03–1.54) and abdominal pain characterised by daily length (OR = 1.33, 95% CI 0.92–1.91), nocturnal occurrence (OR = 1.62, 95% CI 1.19–2.19), spring aggravation (OR = 1.68, 95% CI 0.70–4.05), and no relation to meals (OR = 0.62, 95% CI 0.43–0.91) or stress (OR = 0.69, 95% CI 0.50–0.95). The inclusion of people with increased levels of IgG antibodies to H pylori, but without upper dyspepsia, at study entry significantly increased the likelihood of reporting upper dyspepsia at follow up (OR = 1.71, 95% CI 1.24–2.36). People with epigastric pain and increased levels of IgM antibodies to H pylori only indicative of acute H pylori infection were more likely to report nocturnal pain, heartburn, nausea, and vomiting.Conclusions—H pylori infection may precede the development of dyspepsia and is associated with a variety of gastrointestinal symptoms in people with no history of peptic ulcer disease.

Download Full-text

Helicobacter pylori infection in Iran: demographic, endoscopic and pathological factors

BMC Gastroenterology ◽

10.1186/s12876-021-01931-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Seyedeh Amineh Hojati ◽

Sara Kokabpeyk ◽

Salma Yaghoubi ◽

Farahnaz Joukar ◽

Mehrnaz Asgharnezhad ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Epigastric Pain ◽

Tap Water ◽

Clinical Manifestations ◽

Gastrointestinal Symptoms ◽

Common Symptom ◽

Pathological Findings ◽

Significant Difference ◽

H Pylori

Abstract Background Helicobacter pylori (H. pylori) infection is the most important risk factor for gastritis and peptic ulcer. However, factors other than H. pylori are involved in its pathogenesis. In the current study, we aimed to compare the clinical manifestations and endoscopic and histopathological findings of patients with and without H. pylori infection. Methods In this cross-sectional study, 233 patients with dyspepsia, referred for endoscopy, were examined regarding the presence of H. pylori infection. During an endoscopic exam, 5 biopsy specimens were taken from the stomach. The criteria for the presence of H. pylori infection was the presence and identification of bacteria in pathology. Two groups of H. pylori-positive and H. pylori-negative patients were compared regarding their demographic, endoscopic, and pathological findings. Results Of 233 patients, 154 (66.1%) were non-smokers, 201 (86.3%) were not alcohol users, and 153 (65.7%) used tap water. The most common symptom, reported in 157 (67.4%) patients, was epigastric pain. There was a significant difference between patients with and without H. pylori infection in terms of the educational status, occupational status, family history of gastrointestinal cancer, and some gastrointestinal symptoms. Also, there was a significant relationship between the endoscopic and pathological findings of patients with H. pylori. Conclusions The results of the present study revealed that H. pylori infection was not associated with sex, alcohol consumption, or non-steroidal anti-inflammatory drug use. The role of H. pylori in the pathophysiology of peptic ulcer was clarified. Also, there was a significant difference in the endoscopic and pathological findings of patients with H. pylori.

Download Full-text

Atypical presentation at acute gastritis: significant gastric wall thickening as a presentation of a primary Helicobacter pylori infection in children

BMJ Case Reports ◽

10.1136/bcr-2021-243912 ◽

2021 ◽

Vol 14 (7) ◽

pp. e243912

Author(s):

Kiyokuni Nakamura ◽

Ryo Tamura ◽

Yoshitomi Yasui ◽

Hideaki Okajima

Keyword(s):

Helicobacter Pylori ◽

Epigastric Pain ◽

Gastric Wall ◽

Malignant Tumour ◽

Wall Thickening ◽

Atypical Presentation ◽

Negative Consequences ◽

Ulcer Disease ◽

Acute Gastritis ◽

H Pylori

Helicobacter pylori infection could cause chronic inflammation in the stomach and induce peptic ulcer disease or even malignant tumour. The initial infection of the organism happens in childhood but most of cases are latent. We had a case of 10-year-old girl who presented with acute epigastric pain and significant thickening of the stomach wall on CT. The finding seemed atypical for acute gastritis so oesophagogastroduodenoscopy and serology examination were added and the primary infection of H. pylori was confirmed with the exclusion of other possible diagnoses like eosinophilic gastritis and IgA vasculitis. Acute gastritis is one of the most common sickness in children, however, it would be worthwhile considering further investigation including H. pylori infection in a case of atypical presentation to prevent negative consequences derived from chronic H. pylori infection.

Download Full-text

Prevalence and Associated Symptoms of Helicobacter pylori Infection among Schoolchildren in Kassala State, East of Sudan

Interdisciplinary Perspectives on Infectious Diseases ◽

10.1155/2018/4325752 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 3

Author(s):

Mohammed Abbas ◽

Faiza A. Sharif ◽

Shamselfalah M. Osman ◽

Abdallah M. Osman ◽

Sulieman M. El Sanousi ◽

...

Keyword(s):

Primary Schools ◽

Gastrointestinal Symptoms ◽

Cross Sectional Study ◽

Igg Antibodies ◽

Normal Range ◽

Cross Sectional ◽

Abdominal Symptoms ◽

Associated Symptoms ◽

H Pylori

This study aimed to determine the prevalence of H. pylori infections among schoolchildren and investigate the associations between H. pylori seropositivity and existence of gastrointestinal symptoms. Methods. A prospective cross-sectional study was conducted during a period from January to December 2012 at Kassala state, east of Sudan. Schoolchildren from different primary schools were enrolled in the study. Sociodemographic characteristics and gastrointestinal symptoms were recorded from each child. A rapid immunochromatographic test was performed for the detection of H. pylori IgG antibodies. Data on patient demographic characteristics, clinical diagnosis, and findings of H. pylori infection were analyzed by simple descriptive statistics. Results. Among 431 schoolchildren, H. pylori seropositivity was found to be 21.8%. The majority of children (79; 84%) had BMI below the normal range. The most frequent symptoms associated with H. pylori infections were nausea (25.5%), followed by gastric pain (24.5%) and heart pain (20.2%). There were statistically significant differences in H. pylori seropositivity between boys and girls (p=0.003). Conclusions. The prevalence of H. pylori infection among schoolchildren in Kassala city has been documented. Although the majority of the disease was associated with several gastrointestinal symptoms, the role of infection in the etiology of abdominal symptoms needs further investigations.

Download Full-text

Eradication of Helicobacter pylori may improve dyspepsia in the elderly for the long term

BMC Gastroenterology ◽

10.1186/s12876-021-02027-6 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Ikko Tanaka ◽

Shoko Ono ◽

Yoshihiko Shimoda ◽

Masaki Inoue ◽

Sayoko Kinowaki ◽

...

Keyword(s):

Helicobacter Pylori ◽

Elderly Patients ◽

Epigastric Pain ◽

Eradication Therapy ◽

The Elderly ◽

Long Term Effects ◽

Postprandial Fullness ◽

Abdominal Symptoms ◽

H Pylori

Abstract Background Therapy for eradication of Helicobacter pylori (H. pylori) improves symptoms of H. pylori-associated dyspepsia (HPD), but the effects of eradication in elderly patients are unclear. The aim of our study was to investigate dyspepsia symptoms and long-term effects of eradication in elderly patients. Methods This retrospective study included 496 patients who received H. pylori eradication therapy. The patients were divided into a group of elderly patients (group E: ≧ 65 years old) and a group of non-elderly patients (group N: < 65 years old). Abdominal symptoms were evaluated using a questionnaire about abdominal symptoms before eradication and after eradication (1–2 months and more than one year). Dyspepsia was defined as a score of 4 points or more for at least one of 4 items (postprandial fullness, early satiety, epigastric pain, and hunger pain). Improvement of symptoms was defined on the basis of changes in Global Overall Systems scores. Results There were no differences in abdominal symptoms before eradication between the two groups. Successful eradication improved symptoms in patients with dyspepsia within 2 months (in 75.6% (56/74) of the patients in group N and in 64.5% (20/31) of the patients in group E). The questionnaire showed that 80% (32/40) of the patients in group N and 60% (12/20) of the patients in group E had long-term relief of dyspepsia. The scores for abdominal symptoms in group E continued to improve for a mean period of 54.8 months after eradication. Conclusions Eradication of H. pylori age-independently improved dyspepsia symptoms for the long term.

Download Full-text

Detection of Anti-CagA Antibodies in Sera of Helicobacter pylori-Infected Patients Using an Immunochromatographic Test Strip

Journal of Chromatographic Science ◽

10.1093/chromsci/bmz093 ◽

2019 ◽

Vol 58 (3) ◽

pp. 217-222

Author(s):

Cebrail Karakus ◽

Zeynep Ulupinar ◽

Fahri Akbas ◽

Duygu Yazici

Keyword(s):

Helicobacter Pylori ◽

Test Strip ◽

Mucosal Damage ◽

Enzyme Linked Immunosorbent Assay ◽

Control Line ◽

Immunochromatographic Test ◽

Caga Gene ◽

Serum Samples ◽

Ulcer Disease ◽

H Pylori

Abstract The cagA gene of Helicobacter pylori that encodes an immunodominant CagA protein provokes severe mucosal damage and acts as a risk factor for the development of peptic ulcer disease and gastric cancer. Our aim is to develop an immunochromatographic test strip (ICTS) using our previously developed recombinant CagA (rCagA) protein and anti-rCagA monoclonal antibody (Mab) for the detection of anti-CagA antibodies in sera of infected patients. The rCagA was firstly conjugated to gold nanoparticle and placed into the conjugate pad. A nonconjugated rCagA and anti-rCagA Mab (CK-02) were immobilized on the test line and control line, respectively. Biopsy and serum samples from 30 H. pylori-infected patients were used. The presence of cagA gene in biopsy samples was first detected by PCR (Polymerase Chain Reaction), and 22 patients were found positive while 8 were negative. When serum samples were tested by our developed ICTS, 21 were positive for anti-CagA antibodies while 9 were negative. The serum samples were also tested by a commercial ELISA (Enzyme Linked Immunosorbent Assay), and when compared to the ICTS a sensitivity of 95% and a specificity of 100% were obtained. The ICTS can be used for rapid detection of CagA-positive H. pylori infection instead of expensive, time consuming and laborious invasive approaches.

Download Full-text

Eradication of Helicobacter Pylori Improves Dyspepsia Symptoms in Elderly People

10.21203/rs.3.rs-118169/v1 ◽

2020 ◽

Author(s):

Ikko Tanaka ◽

Shoko Ono ◽

Yoshihiko Shimoda ◽

Masaki Inoue ◽

Sayoko Kinowaki ◽

...

Keyword(s):

Helicobacter Pylori ◽

Elderly Patients ◽

Eradication Rate ◽

Epigastric Pain ◽

Eradication Therapy ◽

Serious Adverse Events ◽

Postprandial Fullness ◽

Abdominal Symptoms ◽

H Pylori ◽

One Year

Abstract BackgroundTherapy for eradication of Helicobacter pylori (H. pylori) improves symptoms of H. pylori-associated dyspepsia (HPD), but the effects of eradication in elderly patients are unclear. AimsTo evaluate the outcomes of eradication therapy and effects of eradication on dyspepsia symptoms in elderly patients.MethodsThis retrospective study included 496 patients who received H. pylori eradication therapy. The patients were divided into a group of elderly patients (group E: ≧ 65 years old) and a group of non-elderly patients (group N: < 65 years old). Abdominal symptoms were evaluated using a questionnaire about 12 abdominal symptoms before eradication and after eradication (1-2 months and more than one year). Dyspepsia was defined as a score of 4 points or more score for at least one of 4 items (postprandial fullness, early satiety, epigastric pain, and hunger pain).ResultsSuccesful H. pylori eradication rate in group E was significantly lower than that in group N (74.7 % vs. 84.4 %, P < 0.05) and it was remarkable in the 3rd-line therapy (59.7 % vs. 76.5 %, P < 0.05). Serious adverse events did not occur in either groups. Successful eradication improved symptoms in patients with dyspepsia in both groups within 2 months (70.3% of the patients in group N, 76.2% of the patients in group E) and decrease of GOS score lasted for more than 1 year.ConclusionsH. pylori eradication would be recommend for elderly patients with dyspepsia symptoms.

Download Full-text

Prevalence of Helicobacter pylori in United States Navy submarine crews

Epidemiology and Infection ◽

10.1017/s0950268805005169 ◽

2005 ◽

Vol 134 (3) ◽

pp. 460-464 ◽

Cited By ~ 3

Author(s):

R. P. JACKMAN ◽

C. SCHLICHTING ◽

W. CARR ◽

A. DUBOIS

Keyword(s):

United States ◽

Helicobacter Pylori ◽

United States Navy ◽

Close Contact ◽

Igg Antibodies ◽

Serum Samples ◽

Us Army ◽

The Us ◽

H Pylori ◽

Army Recruits

Helicobacter pylori prevalence is elevated in German submarine crews and in United States Navy (USN) surface fleet personnel, but H. pylori prevalence in USN submariners was unknown. The goal of the study was to determine the prevalence of H. pylori in the crews of USN nuclear submarines compared to other military personnel and to the general US population. The presence of H. pylori IgG antibodies was determined in serum samples using a commercial ELISA. Only 47 out of 451 submariners (9·4%) were H. pylori positive, which is similar to that of the US general population with a similar level of education. In contrast, H. pylori prevalence is significantly higher in US Army recruits (26%), USN surface fleet personnel (25%), and German diesel submariners (38%). These data demonstrate that submarine service (and by inference activity requiring isolation and close contact, per se) is not a risk factor for H. pylori infection.

Download Full-text

Detection of Anti-VacA Antibody Responses in Serum and Gastric Juice Samples Using Type s1/m1 and s2/m2 Helicobacter pylori VacA Antigens

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.6.4.489-493.1999 ◽

1999 ◽

Vol 6 (4) ◽

pp. 489-493 ◽

Cited By ~ 21

Author(s):

Guillermo I. Perez-Perez ◽

Richard M. Peek ◽

John C. Atherton ◽

Martin J. Blaser ◽

Timothy L. Cover

Keyword(s):

Helicobacter Pylori ◽

Gastric Juice ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Ulcer Disease ◽

Serum Igg ◽

Increased Risk ◽

Antigenic Properties ◽

H Pylori

ABSTRACT Several different families of vacuolating toxin (vacA) alleles are present in Helicobacter pylori, and they encode products with differing functional activities. H. pyloristrains containing certain types of vacA alleles have been associated with an increased risk for peptic ulcer disease. In this study, we tested serum samples and gastric juice from 19 H. pylori-negative and 39 H. pylori-positive patients for enzyme-linked immunosorbent assay reactivity with two different types of VacA antigens (types s1/m1 and s2/m2), which were purified from H. pylori 60190 and 86-338, respectively. Both antigens were recognized better by serum immunoglobulin G (IgG) fromH. pylori-positive persons than by serum IgG from H. pylori-negative persons (P < 0.01). The s1/m1 VacA antigen was better recognized by sera from patients carryingvacA type s1/m1 strains than by sera from patients carryingvacA type s2/m2 or s1/m2 strains (P < 0.01). Conversely, the s2/m2 VacA antigen was better recognized by sera from patients carrying type s2/m2 or s1/m2 strains (P= 0.03). Serum IgG anti-VacA antibodies were present more frequently in patients carrying type s1/m1 strains than in other H. pylori-positive patients (P = 0.0002). In addition, the highest levels of IgA anti-VacA antibodies were detected in the gastric juice of patients carrying type s1/m1 strains. These data indicate that different VacA isoforms have distinct antigenic properties and that multiple forms of VacA elicit antibody responses inH. pylori-positive humans.

Download Full-text

Development of FlexSure® HP—an immunochromatographic method to detect antibodies against Helicobacter pylori

Clinical Chemistry ◽

10.1093/clinchem/44.2.293 ◽

1998 ◽

Vol 44 (2) ◽

pp. 293-298 ◽

Cited By ~ 9

Author(s):

Wayne H Schrier ◽

Ronald J Schoengold ◽

Josefina T Baker ◽

Joyce L Norell ◽

Corey L Jaseph ◽

...

Keyword(s):

Helicobacter Pylori ◽

Breath Test ◽

Solid Phase ◽

Reverse Flow ◽

Test Strip ◽

Urea Breath Test ◽

Igg Antibodies ◽

Ulcer Disease ◽

13C Urea Breath Test ◽

H Pylori

Abstract We describe a solid-phase immunochromatographic serologic test, FlexSure® HP, to detect IgG antibodies against Helicobacter pylori. H. pylori colonize the stomach and proximal duodenum, cause ulcer disease and mucosa-associated lymphoid tissue lymphoma, and have a role in the development of other disorders, including gastric adenocarcinoma. FlexSure HP consists of a test strip, conjugate pad, and absorbent pad, in a novel reverse-flow chromatography format. In these studies, FlexSure HP was demonstrated to be specific for IgG antibodies against H. pylori. The reactive cutoff of the test was consistent with [13C]urea breath test and commercially available ELISAs. FlexSure HP had 94% sensitivity, 88% specificity, and 91% accuracy relative to [13C]urea breath test; and 95% sensitivity, 94% specificity, and 95% overall agreement relative to high-molecular-mass cell-associated protein enzyme immunoassay (HM-CAP EIA). FlexSure HP is a simple-to-perform, visually read test requiring no specialized training, equipment, or instrumentation, and yields rapid, accurate, qualitative results.

Download Full-text

Frequency of rdx A Deletion Mutation Conferring Metronidazole Resistance in Helicobacter pylori

10.51429/ejmm29308 ◽

2020 ◽

Vol 29 (3) ◽

pp. 59-64

Author(s):

Hanaa M. El Maghraby ◽

Samar Mohaseb

Keyword(s):

Helicobacter Pylori ◽

Epigastric Pain ◽

Deletion Mutation ◽

Rapid Urease Test ◽

Rrna Gene ◽

University Hospitals ◽

Metronidazole Resistance ◽

Urease Test ◽

Gastric Biopsies ◽

H Pylori

Background: Metronidazole is one of the antimicrobial drugs that can be used in combination with other drugs for eradication of Helicobacter pylori (H. pylori).Unfortunately, metronidazole resistance in H. plori is an increasing health problem which may be attributed to inactivation of many genes as rdx A gene. Objective: To determine the frequency of rdx A deletion mutation in H. pylori detected in infected patients attending at the Gastroenterology Unit, Zagazig University Hospitals. Methodology: Two gastric biopsies were taken from each enrolled patient by endoscopy. H.pylori detection was done by rapid urease test and polymerase chain reaction (PCR) amplification of 16S rRNA gene. Deletion mutation in rdx A gene was detected by conventional PCR. Results: Out of 134 doubled gastric biopsies obtained from 134 patients, 52.2% were positive for H. pylori. Epigastric pain, vomiting and gastritis were significantly associated with detection of H. pylori infection (p˂ 0.05). Deletion mutation of rdx A gene was detected in 28.6% of H. pylori positive specimens obtained from infected patients. Conclusion: Deletion mutation of rdx A gene is a frequent determinant of rdx A inactivation conferring metronidazole resistance among H. pylori.

Download Full-text