scholarly journals Leucocyte endothelial cell adhesion in indomethacin induced intestinal inflammation is correlated with faecal pH

Gut ◽  
1998 ◽  
Vol 42 (3) ◽  
pp. 380-386 ◽  
Author(s):  
H Arndt ◽  
K-D Palitzsch ◽  
J Schölmerich
Keyword(s):  
Inflammatory Bowel Disease ◽  
Cell Adhesion ◽  
Endothelial Cell ◽  
Shear Rate ◽  
Bowel Disease ◽  
Fold Increase ◽  
Inflammatory Bowel ◽  
Faecal Ph ◽  
Endothelial Cell Adhesion ◽  
Leucocyte Adherence

Background—Recent studies indicate that faecal pH is acidified in patients with inflammatory bowel disease compared with healthy controls. In healthy volunteers, stool pH, faecal flora, and bile acid concentration could be affected by means of elemental diets.Aims—To assess the role of variations of faecal pH on leucocyte endothelial cell adhesion in indomethacin induced long lasting ileitis in rats.Methods—Indomethacin (7.5 mg/kg subcutaneously) was injected twice, 24 hours apart. Rats were either fed with the identical diet before and 10 days after the induction of inflammation until the experiment, or the diet was changed at the time of induction. Ten postcapillary mesenteric venules (30 μm diameter) per animal were observed using intravital microscopy. Macroscopic visible intestinal ulceration was scored and faecal pH of different sections of the small bowel was determined.Results—Small intestinal faecal pH was 8.5 in controls and 8.0 in indomethacin treated animals. Indomethacin significantly changed microcirculatory parameters: there was a 2.3-fold increase in leucocyte adherence, a 3.2-fold increase in leucocyte emigration, and a 20% reduction in shear rate. Application of various diets or diet combinations resulted in variations in faecal pH ranging from 7.8 to 8.8 which were inversely correlated with macroscopic ulcerations (r=−0.67). Leucocyte adherence was attenuated with increased pH and augmented with decreased pH (r=−0.55). Venular wall shear rate was positively correlated with faecal pH (r=0.48) while leucocyte emigration showed no correlation. Leucocyte rolling velocity was not significantly altered. Normalisation of faecal pH by different alkalising drugs induced a significant decrease in leucocyte adherence in standard fed, indomethacin treated rats.Conclusions—Faecal pH is lowered in the indomethacin model of long lasting ileitis in rats, which is similar to human inflammatory bowel disease. Alkalisation of faecal pH due to different diets or alkalising drugs reduces indomethacin induced leucocyte endothelial cell adhesion and macroscopic intestinal damage. These results may provide a rationale for the therapeutic effect of enteral diets in Crohn’s disease.

scholarly journals Altered expression of cell adhesion molecules in uninvolved gut in inflammatory bowel disease

2008 ◽  
Vol 94 (2) ◽  
pp. 341-347 ◽  
Author(s):  
G. M. SCHUERMANN ◽  
A. E. ABER-BISHOP ◽  
P. FACER ◽  
J. C. LEE ◽  
D. S. RAMPTON ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Bowel Disease ◽  
Cell Adhesion Molecules ◽  
Altered Expression ◽  
Inflammatory Bowel

scholarly journals Synthesis and evaluation of 6-heteroarylamino-2,4,5-trimethylpyridin-3-ols as inhibitors of TNF-α-induced cell adhesion and inflammatory bowel disease

MedChemComm ◽  
2018 ◽  
Vol 9 (8) ◽  
pp. 1305-1310 ◽  
Author(s):  
Sang Won Park ◽  
Suhrid Banskota ◽  
Pallavi Gurung ◽  
You Jin Jin ◽  
Han-eol Kang ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Cell Adhesion ◽  
Bowel Disease ◽  
Tnf Α ◽  
Inflammatory Bowel ◽  
Anti Inflammatory

Novel series of anti-inflammatory bowel disease (IBD) agent was identified through TNF-α-induced cell adhesion.

Anti-endothelial cell antibodies in inflammatory bowel disease

1993 ◽  
Vol 38 (3) ◽  
pp. 426-432 ◽  
Author(s):  
T. R. J. Stevens ◽  
S. L. Harley ◽  
J. S. Groom ◽  
G. Cambridge ◽  
B. Leaker ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Endothelial Cell ◽  
Bowel Disease ◽  
Inflammatory Bowel

Expression of platelet-derived endothelial cell growth factor in inflammatory bowel disease

2003 ◽  
Vol 38 (3) ◽  
pp. 229-237 ◽  
Author(s):  
Shinsuke Saito ◽  
Nelson H. Tsuno ◽  
Eiji Sunami ◽  
Nobukazu Hori ◽  
Joji Kitayama ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
Bowel Disease ◽  
Endothelial Cell Growth Factor ◽  
Inflammatory Bowel ◽  
Endothelial Cell Growth

scholarly journals Risk of Colorectal Cancer in Inflammatory Bowel Disease: Prevention and Monitoring Strategies According With Risk Factors

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Enrica Giuffrida ◽  
Michela Mangia ◽  
Alessandro Lavagna ◽  
Enrico Morello ◽  
Maurizio Cosimato ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Virtual Colonoscopy ◽  
Fold Increase ◽  
High Definition ◽  
Endoscopic Techniques ◽  
Colonic Involvement ◽  
Monitoring Strategies ◽  
Inflammatory Bowel

Colorectal cancer (CRC) is slightly increased in inflammatory bowel disease (IBD) patients, with roughly a 2.5-fold increase compared to the general population. Clinical features associated to CRC risks are extent and severity of colonic involvement, disease duration, concomitant primary sclerosing cholangitis (PSC) and/or familial history of CRC in first-degree relatives. Colonic Crohn’s disease (CD) and ulcerative colitis (UC) share similar risks when similar colonic extent is affected. Risk stratification affects outcomes and surveillance programs.Newer endoscopic techniques substantially ameliorated diagnostic performance of endoscopy, and nowadays the standard for CRC surveillance in IBD patients is high-definition endoscopy, with dye-spray or virtual colonoscopy, oriented at targeted (+ random) colonic biopsies.Visible dysplastic lesions should be considered for endoscopic resection, while invisible dysplasia is still a mandatory proctocolectomy indication.Newer endoscopic interventional techniques (endoscopic mucosa resection, EMR, and endoscopic submucosal dissection, ESD) are appropriate therapeutic techniques to be delivered, but long-term risks of cancer should be balanced towards proctocolectomy.

scholarly journals Impact of the myosin heavy chain 9 gene single nucleotide polymorphism on inflammatory bowel disease

2021 ◽  
Author(s):  
eman badr ◽  
Ahmed Ezz El-Arab Abd Al-Aliem ◽  
Elsayed Ibrahem El-Shayeb ◽  
Ahmed Megahed Ahmed Taman ◽  
Abd El-naser Abd El-atty Gadallah
Keyword(s):  
Inflammatory Bowel Disease ◽  
Single Nucleotide Polymorphism ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Bowel Disease ◽  
Fold Increase ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Inflammatory Bowel ◽  
Gene Single Nucleotide Polymorphism

Abstract Background To date, the cause of inflammatory bowel disease (IBD) remains a mystery. A balance between cell proliferation and apoptosis maintains intestinal tissue homeostasis. Dissociation-induced myosin-actin contraction results in stem cell apoptosis. This study aiming to evaluate the influence of the myosin heavy chain 9 (MYH9) gene single nucleotide polymorphism (SNP) on inflammatory bowel disease. Subjects and methods: The study carried on eighty patients with IBD and seventy controls. All participants subjected to history taking, thorough physical examination examination, colonoscopy and laboratory investigations. Genotyping performed for rs3752462 and rs4821480 by SNP assay real-time PCR methods. Results On analyzing rs4821480, The TG and GG genotypes have significant increased distribution among the IBD patients as compared to the controls with 5.3 fold increase in the risk of IBD and higher prevalence of GG genotype in patients with low hemoglobin level and higher BMI. While on analyzing rs3753462 CT and TT genotypes were significantly more frequent in the in the IBD patients as compared to the controls with 4.6 fold increase in the risk of IBD. Conclusion The allele G of rs4821480 and T of rs3753462 of MYH9 gene associated with more susceptibility to IBD.

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