EP224 Low long-chain polyunsaturated fatty acids levels in breast adipose tissue are associated with multifocality in women with positive hormone receptors tumors

TPS1589 Background: The most aggressive breast cancer subtypes tend to be estrogen and progesterone receptor negative (ERPR(-)) and human epidermal growth factor receptor type 2 positive (HER2(+)). Women with these breast cancer subtypes (triple negative, ERPR(-)HER2(+)) tend to experience worse clinical outcomes and have a relatively higher risk of recurrence. Our previous research demonstrated that dietary omega-3 (n-3) fatty acids can significantly inhibit ERPR(-) HER2(+) tumorigenesis in MMTV-HER2/neu transgenic mice fed fish oil vs corn oil-based diets. The fish oil diet group developed 30% fewer breast tumors, had lower Ki67 expression, and experienced less mammary atypia relative to the corn oil diet group. Other studies involving diet, nutrition and breast cancer point to the potentially protective effect of an anti-inflammatory diet on the risk of developing ER(-) and HER2(+) breast cancer, further supporting the evidence that the ERPR(-), HER2(±) subtypes may be highly responsive to this bioactive nutrient. Methods: This is a double-blinded, randomized clinical trial of high dose (~5.4 g EPA+DHA) vs low dose (~0.9 g EPA+DHA in fatty acid mix of the typical American diet) of n-3 fatty acids in breast cancer survivors of ERPR(-), HER2(±) breast cancer. Eligible participants will take 5 capsules/day for 12 months, with cellular samples of breast epithelial and/or adipose tissue obtained by fine needle aspirations of the contralateral breast. The study aims to determine whether n-3 fatty acid supplementation will modify fatty acid metabolite content in breast adipose tissue, modulate cytomorphology and/or cell proliferation in breast epithelial cells, affect DNA methylation patterns, and modulate pro- vs anti-inflammatory gene expression patterns in breast adipose tissue. Correlative aims will evaluate possible associations between factors such as breast adipose tissue, red cell membrane fatty acid profiles, BMI, and reported dietary intake. Sample size of 40 participants per arm was calculated to provide at least 80% power to detect a statistically significant difference for each primary endpoint. This study focuses on women survivors of high risk breast cancer subtypes, specifically triple negative or ERPR(-)HER2(+) disease, who are currently without long term adjuvant options. Eligibility criteria include prior diagnosis of ERPR(-) stage 0 to III breast cancer, ≤5 years from completion of standard therapy.The study was closed to accrual in November 2018; less than 9 months of follow-up remain for active study participants. Clinical trial information: NCT02295059.

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Fish Oil-Derived Long-Chain n-3 Polyunsaturated Fatty Acids Reduce Expression of M1-Associated Macrophage Markers in an ex vivo Adipose Tissue Culture Model, in Part through Adiponectin

Frontiers in Nutrition ◽

10.3389/fnut.2015.00031 ◽

2015 ◽

Vol 2 ◽

Cited By ~ 7

Author(s):

Anna A. De Boer ◽

Jennifer M. Monk ◽

Danyelle M. Liddle ◽

Krista A. Power ◽

David W. L. Ma ◽

...

Keyword(s):

Fatty Acids ◽

Tissue Culture ◽

Adipose Tissue ◽

Polyunsaturated Fatty Acids ◽

Fish Oil ◽

Ex Vivo ◽

Culture Model ◽

Tissue Culture Model ◽

Long Chain

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Long-Chain n-3-to-n-6 Polyunsaturated Fatty Acid Ratios in Breast Adipose Tissue From Women With and Without Breast Cancer

Nutrition and Cancer ◽

10.1207/s15327914nc422_5 ◽

2002 ◽

Vol 42 (2) ◽

pp. 180-185 ◽

Cited By ~ 72

Author(s):

Dilprit Bagga ◽

Karl H. Anders ◽

He-Jing Wang ◽

John A. Glaspy

Keyword(s):

Breast Cancer ◽

Adipose Tissue ◽

Fatty Acid ◽

Polyunsaturated Fatty Acid ◽

Breast Adipose Tissue ◽

Breast Adipose ◽

Long Chain

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Difference in fatty acids composition of breast adipose tissue in women with breast cancer and benign breast disease

Nutrición Hospitalaria ◽

10.20960/nh.165 ◽

2017 ◽

Vol 33 (6) ◽

Cited By ~ 1

Author(s):

Lisiane Lopes Conceição ◽

Mariana De Moura Dias ◽

Milene Cristine Pessoa ◽

Geórgia Das Graças Pena ◽

Maria Carolina Santos Mendes ◽

...

Keyword(s):

Breast Cancer ◽

Fatty Acids ◽

Adipose Tissue ◽

Stearic Acid ◽

Dietary Intake ◽

Benign Breast Disease ◽

Breast Disease ◽

Fatty Acids Composition ◽

Breast Adipose Tissue ◽

Breast Adipose

Introduction: Breast cancer is the second most common cancer in the world, and the most frequent cancer among women. Moreover, there are factors that influence the risk for breast cancer including the age, genetic and endocrine factors, and lifestyle.Objectives: Evaluate the consumption of fatty acids; compare the fatty acids composition in the breast adipose tissue of women with breast cancer and benign breast disease as well as potential risk factors; and describe the genotypic frequency of the Pro12Ala PPARγpolymorphism.Material and Methods: A hospital-based case-control study was conducted including incident cases (n = 38 breast cancer; n = 75 benign breast disease; n = 166 control). Lifestyle features, socioeconomic issues, dietary intake, anthropometry, and blood and tissue data were assessed.Results: No differences were observed for fatty acids intake. Interestingly, lauric acid (P = 0.001), myristic acid (P = 0.036), stearic acid (P = 0.031), and total saturated fatty acids (SFAs) (P=0.048) had lower concentrations in BC than in BBD women, while palmitoleic acid (P = 0.022), erucic acid (P = 0.002), total monounsaturated fatty acids (MUFAs) (P = 0.039) and oleic acid/stearic acid ratio (P = 0.015) increased. There was no significant association between PPARγ polymorphism and studied groups (P = 0.977). The age at first full pregnancy (P = 0.004) was significantly associated with the development BC, whereas BMI (P = 0.005); percentage of body fat (P = 0.024); physical activity (P = 0.036); and age at menarche (P = 0.008), at first full pregnancy (P < 0.001), and of first mammogram (P = 0.018) were significantly associated with the development of BBD.Conclusion: The results suggest a different fatty acids composition of breast adipose tissue, a biomarker of long-term dietary intake, particularly for SFAs, MUFA and 18: 1 n-9/18: 00 ratio. Our findings also show that are differences in the factors related to the development of BC and BBC.

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